Article
Biotechnology & Applied Microbiology
Tongyang Deng, Anquan Shang, Ying Zheng, Lianzhen Zhang, Hong Sun, Wei Wang
Summary: The aim of this study was to explore a new marker for the quantitative detection of bacterial vaginosis (BV) with high sensitivity and specificity. By comparing the differences in bacterial abundance between BV, healthy, and BV-intermediate groups using 16S rRNA-sequencing, candidate markers were identified. Real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was then used to quantitatively detect these candidate markers and evaluate their sensitivity and specificity. Four new indicators for BV detection were discovered, with log L. crispatus/G. vaginalis (L/G) < 0 being the best indicator. A molecular diagnostic system independent of the Nugent Score could have an important impact on the clinical management of BV.
Article
Microbiology
Xiaolu Ma, Xiaoxi Wang, Shengna Ye, Jinnan Liu, Hong Yuan, Nan Wang
Summary: This study analyzed the pathogenic factors and drug sensitivity associated with clinical treatment of bacterial vaginosis (BV) in Northeast China. The results showed that Gardnerella vaginalis is more resistant to metronidazole than clindamycin, and neither antibiotic is able to effectively eradicate vaginal biofilms.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Immunology
Xiaodi Chen, Yune Lu, Tao Chen, Rongguo Li
Summary: The vaginal microbiome is crucial for women's health, with bacterial vaginosis (BV) associated with various adverse gynecologic and obstetric outcomes. Research on diagnosis and treatment strategies for BV is making progress.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Carolina Sanita Tafner Ferreira, Camila Marconi, Cristina M. G. L. Parada, Jacques Ravel, Marcia Guimaraes da Silva
Summary: Sialidase activity in cervicovaginal fluid is associated with changes in bacterial composition, potentially leading to diminished local defenses against pathogens.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Infectious Diseases
Rebecca Jane Joseph, Hooi-Leng Ser, Yi-He Kuai, Loh Teng-Hern Tan, Valliammai Jayanthi Thirunavuk Arasoo, Vengadesh Letchumanan, Lijing Wang, Priyia Pusparajah, Bey-Hing Goh, Nurul-Syakima Ab Mutalib, Kok-Gan Chan, Learn-Han Lee
Summary: Bacterial vaginosis, caused by an imbalance in the vaginal microbiota, affects one-third of women worldwide and is associated with high treatment failure and recurrence rates. Current research is focused on alternative treatments like probiotics and vaginal microbiota transplants to improve treatment outcomes and reduce colonization by pathogenic microbes.
Article
Infectious Diseases
Agnieszka Latka, Leen Van Simaey, Marijke Reynders, Piet Cools, Tess Rogier, Barbara Lebbe, Lorenzo Corsini, Christine Landlinger, Mario Vaneechoutte
Summary: The quantification of living cells in biofilm or after eradication treatments is problematic. This study assessed the performance of pre-treatment with propidium monoazide (PMAxx) to prevent qPCR-based amplification of DNA from killed cells. The optimized PMAxx treatment condition showed a significant reduction of Gardnerella cells, validating its use in vaginal samples from women with bacterial vaginosis.
Article
Veterinary Sciences
Jinok Kwak, Sriniwas Pandey, Jinho Cho, Minho Song, Eun Sol Kim, Hyunok Doo, Gi Beom Keum, Sumin Ryu, Yejin Choi, Juyoun Kang, Sheena Kim, Jeongyun Kim, Hyeun Bum Kim
Summary: This study developed a mouse model for human BV induced by G. vaginalis, and demonstrated comparable clinical attributes to patients with BV.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Immunology
Bingbing Xiao, A. Disi, Hanyu Qin, Lan Mi, Dai Zhang
Summary: Mixed vaginitis is a condition where different types of vaginal pathogens coexist. Bacterial vaginosis (BV) plus vulvovaginal candidiasis (VVC) is the most common form. There is limited research on the clinical manifestations, pathogenesis, diagnostic criteria, and therapy of mixed vaginitis. This study investigated the effect of combined drug treatment on the vaginal microbiome in BV+VVC mixed vaginitis patients and found that it can alter the composition of the microbiome, with increased abundance of Lactobacillus improving the prognosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Hanyu Qin, Yun Liu, Zhengyuan Zhai, Bingbing Xiao
Summary: This study found that different subgroups of Gardnerella exhibit differences in biofilm formation ability and antibiotic resistance. Strains with strong biofilm ability contain more genes involved in carbohydrate synthesis and metabolism. These findings contribute to the development of novel treatments for bacterial vaginosis.
Article
Infectious Diseases
Abebaw Bitew, Abeba Mengist, Habtamu Belew, Alemayehu Reta, Felege Hiwot
Summary: The study conducted at Felege Hiwot Referral Hospital found a prevalence rate of 39.5% for bacterial vaginosis among women of reproductive age, with S. aureus, G. vaginalis, S. agalactiae, and E. coli being the predominant bacteria. The research also highlighted antibiotic resistance in certain bacteria causing bacterial vaginosis.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Soo-Im Choi, GaYeong Won, YongGyeong Kim, Chang-Ho Kang, Gun-Hee Kim
Summary: This study investigated the effects of a mixture of five lactobacilli strains on bacterial vaginitis (BV) in HeLa cells and GV-infected BV mice. The findings suggest that this mixture can inhibit the growth of GV, reduce inflammation, and suppress the exfoliation of vaginal epithelial cells.
Article
Immunology
Rui Zhou, Jingjing Lu, Jun Wang, Bingbing Xiao
Summary: Seven Gardnerella genomospecies were detected in Chinese BV patients, but no association between their distribution and BV occurrence or prognosis was found. The relative abundance of L. iners was higher in cured patients, and its antimicrobial activity against G. vaginalis and F. vaginae was validated.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ling Fang, Ruonan Ma, Xuejiao J. Gao, Lei Chen, Yuan Liu, Yanwu Huo, Taotao Wei, Xiaonan Wang, Qian Wang, Haojue Wang, Chengjun Cui, Qifeng Shi, Jing Jiang, Lizeng Gao
Summary: Bacterial vaginosis (BV) is a common vaginal infection worldwide, and drug-resistance has led to recurrence after antibiotic treatment. Metastable iron sulfides have been found to kill drug-resistant G. vaginalis and have the potential for BV treatment.
Article
Microbiology
L. Ruffier d'Epenoux, E. Tessier, A. Guillouzouic, E. Fayoux, C. Bourigault, P. Bemer, S. Corvec
Summary: There is a possibility of introducing a new molecular test in the routine diagnosis of bacterial vaginosis. This study proposes a combination of Nugent scoring and molecular testing for diagnosing bacterial vaginosis, especially for cases with intermediate Nugent scores.
MICROBIOLOGY SPECTRUM
(2022)
Review
Immunology
Hanyu Qin, Bingbing Xiao
Summary: Bacterial vaginosis (BV) is a common infectious disease in women of reproductive age, characterized by an imbalance in the vaginal microbiome. Gardnerella spp. are considered a major cause of BV, but they can also be detected in the vaginal microbiome of healthy women. Recent studies have identified three new species of Gardnerella. This review discusses the types, genetic diversity, and correlation between different Gardnerella spp. and BV.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)