Article
Cell & Tissue Engineering
Wen Kang, Yiwen Wang, Jiaying Li, Weige Xie, Dan Zhao, Li Wu, Hongwei Wang, Sijing Xie
Summary: This study found that TAS2R was significantly upregulated in inflammatory dental pulp tissues, and the expression of TAS2R subtypes increased in hDPSCs under stimulation. TAS2R promoted odontoblastic differentiation of hDPSCs by mediating the increase in intracellular Ca2+.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Wushuang Huang, Qi Huang, Hongwen He, Fang Huang
Summary: This study aimed to investigate the effect and potential mechanism of PER2 on the differentiation of human dental pulp stem cells (hDPSCs). The results showed that PER2 promoted the odontoblastic/osteogenic differentiation of hDPSCs, and this process involved mitochondrial metabolic changes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Jinjin Lu, Rui Li, Shilei Ni, Yuandong Xie, Xinchen Liu, Kai Zhang, Yi Li
Summary: The synthesized MCDs in this study achieved efficient odontoblastic differentiation of hDPSCs in vitro, improving the cell differentiation capacity by promoting the expression of odontoblast genes and proteins, and enhancing the process through autophagy mechanism.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ke Xu, Qin Liu, Wushuang Huang, Yanhao Chu, Wenguo Fan, Jiawei Liu, Yifan He, Fang Huang
Summary: This study investigated the role of FBXO32 in the odontoblastic differentiation of hDPSCs and found that FBXO32 promotes the differentiation process. The findings provide new insights into the regulatory mechanisms of odontoblastic differentiation in hDPSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Kajohnkiart Janebodin, Rakchanok Chavanachat, Aislinn Hays, Morayma Reyes Gil
Summary: The study aimed to investigate the role of VEGFR-2 in odontoblast differentiation of dental pulp stem cells (DPSCs) by knocking down the expression of VEGFR-2. The results showed that VEGFR-2 is crucial for odontoblast differentiation in DPSCs, and deficiency of VEGFR-2 may impair the differentiation of odontoblast cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Lifen Li, Yang Wen, Long Jiang, Ya-Qin Zhu
Summary: The study demonstrated the involvement of the PERK-eIF2 alpha-ATF4 pathway in odontoblastic differentiation of human dental pulp cells (DPCs). Knockdown of PERK gene decreased ALP activity and matrix mineralization in DPCs, indicating the importance of PERK in this process.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Deao Gu, Hanxiao Liu, Xinyi Qiu, Yijun Yu, Xuna Tang, Chao Liu, Leiying Miao
Summary: This study found that erythropoietin can enhance the odontoblastic differentiation of human-derived pulp stem cells and promote the formation of tertiary dentin. This effect is mediated by the EphB4-mediated MAPK signaling pathway, and can be attenuated by EphB4 inhibitors.
Article
Dentistry, Oral Surgery & Medicine
Yoon-Jung Kim, Won-Jae Kim, Sun-Woong Bae, Sun-Mi Yang, Sam-Young Park, Seon-Mi Kim, Ji-Yeon Jung
Summary: Mineral trioxide aggregate (MTA) induces odontoblastic differentiation and mineralization in human dental pulp cells (HDPCs) by modulating autophagy and activating AMPK. Autophagy regulation provides new insights into regenerative endodontic therapy using MTA treatment.
INTERNATIONAL ENDODONTIC JOURNAL
(2021)
Article
Cell Biology
Xiaohui Lu, Jiawen Zhang, Yuanzhou Lu, Jing Xing, Min Lian, Guijuan Feng, Dan Huang, Chenfei Wang, Nimei Shen, Xingmei Feng
Summary: The study revealed that LncKCNQ1OT1 promotes the odontoblastic differentiation of DPSCs by regulating the hsa-miR-153-3p/RUNX2 axis, which may offer a therapeutic target for odontogenesis.
Article
Dentistry, Oral Surgery & Medicine
Wei Zhang, Xiao Yuan
Summary: This study investigated the role of miR-20a in DPSCs, revealing that up-regulated miR-20a enhanced cell viability, migration, osteogenic/odontoblastic differentiation potential, and regulated the NF-kappa B/p65 signaling pathway by targeting IL-8. The findings suggest that miR-20a may serve as a potential treatment target for dental diseases.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
C. Liu, J. Yu, B. Liu, M. Liu, G. Song, L. Zhu, B. Peng
Summary: The study investigated the effects of BACH1 on the proliferation and odontoblastic differentiation of hDPSCs in vitro. Manipulation of BACH1 expression may provide an opportunity to promote the regenerative capacity of hDPSCs.
Article
Dentistry, Oral Surgery & Medicine
Jiaxin Yin, Jue Xu, Ran Cheng, Meiying Shao, Yuandong Qin, Hui Yang, Tao Hu
Summary: This study found that connexin 43 (Cx43) may be involved in maintaining odontoblast arrangement patterns and influencing pulp repair outcomes by regulating odontoblastic differentiation.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2021)
Article
Cell & Tissue Engineering
Xinghong Luo, Weiqing Feng, Shijiang Huang, Shenghong Miao, Tao Jiang, Qian Lei, Jingyao Yin, Sheng Zhang, Xiaochun Bai, Chunbo Hao, Weizhong Li, Dandan Ma
Summary: mTORC1 activation inhibits odontoblastic differentiation of dental pulp stem cells by suppressing exosome release from odontoblasts. These findings provide new insights into dental pulp complex regeneration.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell & Tissue Engineering
Changzhou Li, Xinghuan Xie, Zhongjun Liu, Jianhua Yang, Daming Zuo, Shuaimei Xu
Summary: The study demonstrates that Neu5Ac can promote the osteo-/odontoblastic differentiation of DPSCs by modulating the activity of the ERK signaling pathway, enhancing their mineralization ability and alkaline phosphatase activity.
STEM CELLS INTERNATIONAL
(2021)
Article
Dentistry, Oral Surgery & Medicine
Francine Lorencetti da Silva, Giuliana de Campos Chaves Lamarque, Fernanda Maria Machado Pereira Cabral de Oliveira, Paulo Nelson-Filho, Lea Assed Bezerra da Silva, Raquel Assed Bezerra Segato, Lucia Helena Faccioli, Francisco Wanderley Garcia Paula-Silva
Summary: The study found that LTB4 can promote the differentiation of dental pulp stem cells and mineralization of teeth by regulating the expression of Ibsp and Runx2 genes. Microspheres loaded with LTB4 can continuously release LTB4 in long-term culture and achieve the differentiation and mineralization of dental pulp stem cells.
Article
Pharmacology & Pharmacy
Xinyu Peng, Qi Han, Xuedong Zhou, Yanyan Chen, Xiaoyu Huang, Xiao Guo, Ruiting Peng, Haohao Wang, Xian Peng, Lei Cheng
Summary: Dental caries is a preventable infectious disease caused by interactions between oral bacteria and dietary sugars. pH-sensitive drug delivery systems have become innovative materials for caries prevention. In this study, a pH-sensitive carrier (poly(DMAEMA-co-HEMA)) was used to synthesize a nanomaterial loaded with chlorhexidine (p(DH)@CHX), which exhibited good pH sensitivity and sustained release of CHX in an acidic environment. It showed lower cytotoxicity than free CHX and had the same antibacterial effects on biofilms. In addition, it had the potential to alter oral microbiota composition and reduce caries risk.
Article
Biochemistry & Molecular Biology
Yanping Zuo, Qiuchan Xiong, Qiwen Li, Bin Zhao, Fei Xue, Luxuan Shen, Hanwen Li, Quan Yuan, Shuqin Cao
Summary: Supramolecular hydrogels based on amphiphilic peptide have good biocompatibility and manipulable physicochemical properties, which can effectively promote bone defect regeneration and reconstruction.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Yuting Liu, Yaqian Chen, Yuan Wang, Shuang Jiang, Weimin Lin, Yunshu Wu, Qiwen Li, Yuchen Guo, Weiqing Liu, Quan Yuan
Summary: ALKBH1 is crucial for adipogenic differentiation by regulating the HIF-1 signaling pathway.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Review
Cell Biology
Haoran Chen, Yujia Cui, Demao Zhang, Jing Xie, Xuedong Zhou
Summary: This review systematically summarizes studies published in the past 5 years that assess the relationship between FGF-8 and chondrocytes, revealing the regulatory role of FGF-8 in chondrocyte proliferation, differentiation, survival, and migration. Based on the analysis of existing research results, a therapeutic regimen targeting FGF-8 is proposed to explore the possibility of treating chondrocyte-related diseases.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Dentistry, Oral Surgery & Medicine
R. T. Peng, Y. Sun, X. D. Zhou, S. Y. Liu, Q. Han, L. Cheng, X. Peng
Summary: This study found that Treponema denticola can invade oral squamous cell carcinoma (OSCC) cells and directly promote cell proliferation, regulate the cell cycle, and inhibit apoptosis. It also promotes OSCC tumor growth in mice and upregulates Ki67 expression. The mechanism involves activation of the TGF-beta pathway. Treponema denticola may play an important role in the development of OSCC.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Dentistry, Oral Surgery & Medicine
Yujia Cui, Jing Xie, Linyi Cai, Demao Zhang, Jianxun Sun, Xuedong Zhou
Summary: This study suggests that berberine repairs bone destruction caused by apical periodontics by reducing osteoclast activity, promoting osteoblast differentiation, and inhibiting inflammatory response.
Article
Clinical Neurology
Yachuan Zhou, Xiaoqian Chen, Qinglu Tian, Jun Zhang, Mian Wan, Xin Zhou, Xin Xu, Xu Cao, Xuedong Zhou, Liwei Zheng
Summary: Deletion of apoE gene accelerates lumbar spine degeneration, leading to cartilage endplate degradation and sclerosis. The deletion of apoE stimulates abnormal activation of adipokines signals.
Article
Pharmacology & Pharmacy
Yingke Liu, Xingying Qi, Yashi Wang, Man Li, Quan Yuan, Zhihe Zhao
Summary: Oral mucositis (OM) is a common complication of cancer chemotherapy, and its treatment is challenging. In this study, we developed nanomicelles that target inflammation and loaded them with the anti-inflammatory drug cannabidiol (CBD). These nanomicelles showed improved accumulation and retention of CBD, and demonstrated better anti-inflammatory effects compared to free CBD in both local and systemic administration. They also accelerated healing and inhibited inflammatory cell infiltration and transcription. Overall, CBD-loaded nanomicelles show promise for OM treatment.
Article
Dentistry, Oral Surgery & Medicine
Q. Wang, W. Lin, X. Zhou, K. Lei, R. Xu, X. Zhang, Q. Xiong, R. Sheng, W. Song, W. Liu, Q. Wang, Q. Yuan
Summary: This study used single-cell RNA sequencing to investigate gingival heterogeneity in type 2 diabetes patients and found that immune-like fibroblasts play a significant role in immune homeostasis at the diabetic gingiva.
JOURNAL OF DENTAL RESEARCH
(2022)
Review
Dentistry, Oral Surgery & Medicine
Xian Peng, Lei Cheng, Yong You, Chengwei Tang, Biao Ren, Yuqing Li, Xin Xu, Xuedong Zhou
Summary: Oral bacteria directly influence dental caries and periodontal diseases, and the dynamic oral microbiota reflects immune and metabolic information and status through communication between the oral cavity and systemic organs. The oral cavity acts as an important interaction window between the human body and the environment, and the microbial composition in different sites is regulated by complex signaling, hosts, and external environmental factors.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2022)
Review
Dentistry, Oral Surgery & Medicine
Lei Cheng, Lu Zhang, Lin Yue, Junqi Ling, Mingwen Fan, Deqin Yang, Zhengwei Huang, Yumei Niu, Jianguo Liu, Jin Zhao, Yanhong Li, Bin Guo, Zhi Chen, Xuedong Zhou
Summary: Dental caries is a chronic oral disease that poses a significant threat to human health. Despite efforts by dentists and researchers, the prevalence of dental caries remains high. Establishing a clinical difficulty assessment system for caries prevention and management based on diagnosis and classification is crucial. Dentists should conduct oral examinations and create dental records during each visit. Personalized treatment plans, based on caries risk assessment and lesion activity, are necessary to restore oral microecological balance, control caries progression, and restore the structure and function of the affected teeth. Follow-up visits should be conducted based on personalized caries management. This expert consensus focuses on caries risk assessment, treatment difficulty assessment, and dental caries treatment plans, which are essential components of caries management throughout the life cycle.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2022)
Review
Immunology
Hao Dong, Jiaxin Liu, Jianhui Zhu, Zhiyan Zhou, Marco Tizzano, Xian Peng, Xuedong Zhou, Xin Xu, Xin Zheng
Summary: Taste receptors play a role in the interaction between oral microbiota and the host, affecting the occurrence and development of oral diseases.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Cell Biology
Bin Zhao, Yuning Zhang, Jie Xu, Yuyu Li, Quan Yuan, Chenchen Zhou
Summary: This study demonstrates that Periplaneta americana extract (PAE) inhibits osteoclastogenesis and reduces the expression and secretion of inflammatory factors, suggesting its potential therapeutic alternative for bone diseases.
CELL PROLIFERATION
(2023)
Article
Microbiology
Yangyang Shi, Jingou Liang, Xuedong Zhou, Biao Ren, Haohao Wang, Qi Han, Hao Li, Lei Cheng
Summary: The study found that DMAEM@RA had a pH-responsive antimicrobial effect on Streptococcus mutans and Candida albicans dual-species biofilms, preventing secondary caries and attenuating cariogenic virulence.
Article
Multidisciplinary Sciences
Kexin Lei, Ruoshi Xu, Qian Wang, Qiuchan Xiong, Xinyi Zhou, Qiwen Li, Dutmanee Seriwatanachai, Shuibin Lin, Chenchen Zhou, Quan Yuan
Summary: METTL5 is a methyltransferase involved in the modification of eukaryotic 18S ribosomal RNA m6A and its mutations are associated with intellectual disability, microcephaly, and facial dysmorphism in patients. This study shows that Mettl5 knockout mice exhibit poor ossification, widened cranial sutures, and a cleidocranial dysplasia-like phenotype. Deletion of Mettl5 leads to increased proliferation and decreased osteogenic differentiation of suture mesenchymal stem cells. Mechanistically, Wnt signaling is significantly downregulated after Mettl5 knockout. Overall, this research highlights the critical role of METTL5 in craniofacial development and osteogenic differentiation, suggesting its potential as a diagnostic and therapeutic target for craniofacial developmental diseases.
FUNDAMENTAL RESEARCH
(2023)