Journal
BMC BIOLOGY
Volume 17, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12915-019-0682-0
Keywords
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Categories
Funding
- NSF [1644405]
- NEI [DP1EY024503, R01EY011787]
- NIMH [R01MH115900]
- NINDS [R01NS110422]
- US Army Research Laboratory
- US Army Research Office [W911NF-12-1-0594]
- Pew Charitable Trust
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1644405] Funding Source: National Science Foundation
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As a holy grail of neuroscience, optical imaging of membrane potential could enable high resolution measurements of spiking and synaptic activity in neuronal populations. This has been partly achieved using organic voltage-sensitive dyes in vitro, or in invertebrate preparations yet unspecific staining has prevented single-cell resolution measurements from mammalian preparations in vivo. The development of genetically encoded voltage indicators (GEVIs) and chemogenetic sensors has enabled targeting voltage indicators to plasma membranes and selective neuronal populations. Here, we review recent advances in the design and use of genetic voltage indicators and discuss advantages and disadvantages of three classes of them. Although genetic voltage indicators could revolutionize neuroscience, there are still significant challenges, particularly two-photon performance. To overcome them may require cross-disciplinary collaborations, team effort, and sustained support by large-scale research initiatives.
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