Journal
BIORESOURCE TECHNOLOGY
Volume 285, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.121346
Keywords
Biorefinery; Fractionation; Lignocellulose; Oligosaccharides; Enzymatic hydrolysis
Funding
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil)
- Fundacao para a Ciencia e a Tecnologia (FCT, Portugal) [IF/00471/2015]
- CAPES (Brazil) [CAPES 371/14, 0005/2015, FCT/1909/27/2/2014/S, 23038.002463/2014-98]
- FCT/MEC (Portugal) [CAPES 371/14, 0005/2015, FCT/1909/27/2/2014/S, 23038.002463/2014-98]
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The combination of hydrothermal pretreatment followed by delignification with imidazole was evaluated for the first time as a potential selective two-stage fractionation for elephant grass, aiming at obtaining pure fractions susceptible to conversion to high value-added products. In addition, the recovery of cellulose and hemicelluloses and enzymatic hydrolysis yield of pretreated elephant grass were evaluated. Hydrothermal pretreatment at 180 degrees C under non-isothermal conditions allowed obtaining a liquor rich mainly in xylo- and glucooligo-saccharides, as well as pentoses. Subsequent treatment of the recovered solid fraction with imidazole at 140 degrees C for 182.5 min resulted in 83.8 wt% delignification and cellulose enrichment of 97.7 wt%. The solids obtained from the two-stage pretreatment process also permitted high glucan to glucose conversion through enzymatic hydrolysis using Cellic CTec2 (99.0 mol%) or an enzymatic complex of Penicillium echinulatum (96.3 mol%).
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