Article
Biotechnology & Applied Microbiology
G. M. Frolova, E. R. Kotlova, S. V. Sokornova, S. V. Senik, A. L. Shavarda, A. D. Misharev, A. O. Berestetskiy
Summary: The study analyzed changes in pathogenicity and lipid profiles of Stagonospora cirsii VIZR 1.41 during submerged cultivation to identify potential qualitative markers for a mycoherbicide against Canada thistle. Results showed that specific structural lipids, such as phosphatidylcholine, phosphatidylethanolamine, and ergosterol, were associated with high pathogenicity at the log phase of growth, while triacylglycerides were most abundant during the beginning of stationary growth phase. The double-bond index of fatty acids in glycerolipids decreased with culture age, indicating a change in lipid composition and pathogenicity.
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
(2021)
Article
Marine & Freshwater Biology
Nathan Hubot, Sarah L. C. Giering, Cathy H. Lucas
Summary: This study analyzed the biochemical composition of the body and mucus of five scyphozoan jellyfish species and found that protein was the major component in all samples, followed by lipids and carbohydrates. The study also provided valuable ratios to support the integration of jellyfish into trophic and biogeochemical models.
JOURNAL OF PLANKTON RESEARCH
(2022)
Review
Microbiology
Tushar Mehta, Mukesh Meena, Adhishree Nagda
Summary: Curvularia species are filamentous fungi that produce bioactive compounds with various biological activities, including anti-cancer, anti-inflammatory, anti-microbial, anti-oxidant, and phytotoxicity. Some species of Curvularia also have negative impacts on humans and animals. However, they also have beneficial implications such as enzyme production and bioherbicides. This review provides significant information about the diversity of secondary metabolites, their biological activities, and the biotechnological implications of Curvularia species.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Vsevolod Dubovik, Anna Dalinova, Alexander Berestetskiy
Summary: Ten-membered lactones (TMLs) are a diverse group of natural polyketides that are abundant in fungi, bacteria, marine organisms, and insects. These compounds exhibit a wide range of biological activities, including phytotoxic, cytotoxic, antifungal, antibacterial, and others. However, there is still a lack of common understanding regarding the natural sources, structural classification, and most characteristic biological activities of TMLs. The problems include controversial nomenclature, incorrect structure elucidation, poor identification of producing organisms, and scattered information on the biological activity of compounds.
NATURAL PRODUCT REPORTS
(2023)
Review
Biochemistry & Molecular Biology
Antonio Evidente
Summary: This review focuses on macrolides isolated from fungal sources, examining their biological activities, stereochemistry, and structure-activity relationships. The review provides a critical evaluation of recent research in this area, as well as the chemical and biological characterization of fungal macrolides isolated over the past four decades. The content of this review is of interest to chemists, plant pathologists, mycologists, biologists, and pharmacologists, as well as farmers, agri-food specialists, and professionals in the medicinal and cosmetic industries. The practical application of macrolides could have a positive impact on environmental pollution, consumer satisfaction, and human and animal health.
NATURAL PRODUCT REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
K. V. Ajayan, K. Saranya, C. C. Harilal
Summary: This study investigated the effects of Indole-3-butyric acid (IBA) supplementation combined with nitrogen limitation on the biomass, biochemical and fatty acid composition of three selenastracean microalgae. The results showed that IBA supplementation increased the biomass production and influenced the synthesis of chlorophyll and carotenoids. The combination of nitrogen limitation and IBA significantly increased the lipid content and provided valuable feedstock for various industries.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Lana Flanjak, Ivna Vrana, Ana Cvitesic Kusan, Jelena Godrijan, Tihana Novak, Abra Penezic, Blazenka Gasparovic
Summary: Environmental stresses, such as temperature increase and nitrogen deficiency, have significant impacts on the physiological and biochemical processes of marine diatom Chaetoceros pseudocurvisetus. The response to these stresses varies, with nitrogen limitation affecting cell growth and reproduction, and high temperature influencing cell death and lipid remodelling.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Agronomy
Juozas Lanauskas, Nobertas Uselis, Loreta Buskiene, Romas Mazeika, Gediminas Staugaitis, Darius Kviklys
Summary: This study investigated the effects of cattle horn shavings on apple tree nitrogen nutrition, observing that spring application of horn shavings was less effective compared to other fertilisers. The lowest yield was found in unfertilised orchard soil, while the highest yield was recorded from fruit trees fertilised with ammonium nitrate in spring or horn shavings in autumn. Horn shavings showed similar effects to ammonium nitrate on other parameters studied, indicating its potential use for nitrogen nutrition management in organic orchards.
Article
Materials Science, Multidisciplinary
O. Olaye, O. A. Ojo
Summary: This study examines the validity of a long-held implicit assumption that the composition-dependent interdiffusion coefficient remains isothermally constant regardless of solute-source composition. The results of numerical simulations and experimental analyses show that solute concentration can significantly affect the composition dependency of interdiffusion coefficient, contrary to previous assumptions. Therefore, using concentration-dependent interdiffusion functions obtained from pure-metal/pure-metal diffusion couples to predict or analyze diffusion effects in alloy solute sources can be highly inaccurate.
RESULTS IN PHYSICS
(2022)
Article
Environmental Sciences
Meredith G. Lassiter, Jiajia Lin, Jana E. Compton, Jennifer Phelan, Robert D. Sabo, John L. Stoddard, Stephen R. McDow, Tara L. Greaver
Summary: The composition of atmospheric nitrogen deposition in the United States is changing spatially and temporally, with reduced nitrogen now equivalent to oxidized nitrogen in national average deposition. To assess the effects of this change on stream chemistry, data on nitrogen deposition form and stream measurements were analyzed. It was found that there is a shift from oxidized nitrogen dominance to reduced nitrogen dominance in atmospherically-influenced watersheds. The correlation between reduced nitrogen deposition and stream nitrate concentrations was weaker compared to oxidized nitrogen deposition.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Geochemistry & Geophysics
Yuhan Lin, Shaoqing Wang, Harold H. Schobert, Qingjun Guo, Xueqi Li
Summary: The geochemical characteristics of sulfur and nitrogen contents and isotopic compositions in a Late Permian bark coal in southern China were studied. The results showed the influence of precursor plants on sulfur occurrence and the potential microbial degradation during the peat accumulation stage. The differences in delta S-34 and delta N-15 values suggested different coal-forming systems for different coal seams.
ORGANIC GEOCHEMISTRY
(2022)
Article
Plant Sciences
Victor Murison, Josiane Herault, Martine Come, Sabrina Guinio, Alexis Lebon, Christophe Chamot, Magalie Benard, Ludovic Galas, Benoit Schoefs, Justine Marchand, Muriel Bardor, Lionel Ulmann
Summary: This study investigated the accumulation and degradation of lipid droplets in two ecotypes of P. tricornutum under nitrogen deprivation and resupply conditions. The results showed that lipid accumulation may be limited by the size of the plastid, and a possible migration of lipid droplets to vacuoles was observed, suggesting autophagic degradation.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Environmental Sciences
Yasheng Shi, Ping Tian, Zanfang Jin, Yuming Hu, Yongqi Zhang, Feili Li
Summary: The study found that the stable nitrogen isotopes values of nitrogen oxides emitted from biomass burning in China are influenced by the biomass fuel sources, leading to increased uncertainty in the national scale model due to limited data on δ N-15-NOx values.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Energy & Fuels
Jayati Trivedi, Deepti Agrawal, Neeraj Atray, Anjan Ray
Summary: The present study optimized nitrate concentration in a flat panel photobioreactor to achieve high lipid accumulation of Scenedesmus obliquus CCAP 276/3A and a FAME profile suitable for biodiesel production. The results showed that Scenedesmus obliquus CCAP 276/3A is a suitable feedstock for biodiesel production, and provided important insights into enhancing lipid production in algae.
Article
Agriculture, Multidisciplinary
Jiao Wang, Lingyu Ouyang, Li Wei
Summary: The study reveals that the protein expression and lactylation of Nannochloropsis oceanica undergo changes under nitrogen stress. Using the 4D label-free proteome method, it was found that there are 868 lactylation sites in the proteins, which are linked to biological activities such as lipid metabolism and photosynthesis. This study provides a foundation for future epigenetic enhancement of microalgal cells by providing the first dataset of lactylome in Nannochloropsis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
G. M. Frolova, E. R. Kotlova, S. V. Sokornova, S. V. Senik, A. L. Shavarda, A. D. Misharev, A. O. Berestetskiy
Summary: The study analyzed changes in pathogenicity and lipid profiles of Stagonospora cirsii VIZR 1.41 during submerged cultivation to identify potential qualitative markers for a mycoherbicide against Canada thistle. Results showed that specific structural lipids, such as phosphatidylcholine, phosphatidylethanolamine, and ergosterol, were associated with high pathogenicity at the log phase of growth, while triacylglycerides were most abundant during the beginning of stationary growth phase. The double-bond index of fatty acids in glycerolipids decreased with culture age, indicating a change in lipid composition and pathogenicity.
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
(2021)
Article
Plant Sciences
Sophie Sokornova, Tatiana Matveeva
Summary: The Antirrhineae tribe of Plantaginaceae family is well known for its various garden ornamentals and medicinal plants. The Linaria genus is famous for containing naturally-transgenic plants, and iridoid glycosides such as antirrhinoside play a major role in the tribe. Despite differences in research level among the tribe representatives, antirrhinoside presence serves as a marker for this group of plants.
PHYTOCHEMISTRY REVIEWS
(2022)
Review
Microbiology
Alexander Berestetskiy, Qiongbo Hu
Summary: This review discusses the increasing use of biorational insecticides of natural origin in agriculture, and highlights the importance of using a chemical ecology approach to search for new compounds with insecticidal properties produced by fungi. Different ecological groups of fungi were found to produce substances with insecticidal and antifeedant properties, with soil fungi, specifically Aspergillus and Penicillium genera, being the main producers. Additionally, mushrooms were identified as promising sources of antifeedant compounds and insecticidal proteins.
Article
Microbiology
Fei Yin, Miaomiao Xiao, Alexander Berestetskiy, Qiongbo Hu
Summary: This study identified transmembrane protein 214 (BmTEME214) and protein transport protein SEC23A isoform X2 (BmSEC23) as potential Destruxin A (DA)-binding proteins and validated their interaction through bio-layer interferometry (BLI), insect two-hybrid (I2H), and RNAi experiments. The results demonstrated strong affinity between DA and BmTEME214 and BmSEC23 proteins, shedding light on the action mechanism of DA.
Article
Microbiology
Jingjing Wang, Alexander Berestetskiy, Qiongbo Hu
Summary: Destruxin A (DA) is a mycotoxin produced by the fungus Metarhizium anisopliae with insecticidal activities, showing affinity to the six aminoacyl tRNA synthetases of Bombyx mori. This interaction inhibits protein synthesis and decreases free amino acid content in cells. The study provides insights into the molecular target of DA against target insects.
Article
Microbiology
Dilara Salimova, Anna Dalinova, Vsevolod Dubovik, Igor Senderskiy, Elena Stepanycheva, Oksana Tomilova, Qiongbo Hu, Alexander Berestetskiy
Summary: Research on fungal antibiotics from Alternaria tenuissima MFP253011 extracts revealed potential unidentified compounds with entomotoxic activity, particularly Tenuazonic acid (TeA) which showed strong larvicidal effects on Galleria mellonella. Besides, TeA also exhibited toxicity against other insects and cell lines.
Article
Microbiology
Anna Dalinova, Anatoly Fedorov, Vsevolod Dubovik, Olga Voitsekhovskaja, Elena Tyutereva, Sergey Smirnov, Dmitry Kochura, Leonid Chisty, Igor Senderskiy, Alexander Berestetskiy
Summary: Ten-membered lactones demonstrate phytotoxic, antimicrobial, and fungicidal activity, with Stagonospora cirsii fungus producing phytotoxic stagonolides with high yield. The study aimed to create structurally related nonenolides and identify the structural features that affect their biological activity. The oxidation of C-7 hydroxyl group was found to be a key structural feature affecting the phytotoxicity of nonenolides, with 7S compounds showing higher phytotoxicity compared to their 7R analogues.
Review
Plant Sciences
Shaza S. Issa, Sofia Sokornova, Roman R. Zhidkin, Tatiana Matveeva
Summary: This review discusses various methods to search for potential inhibitors of the main protease of coronavirus (Mpro), including virtual screening, high-throughput screening, and cell-based antiviral activity assays. It focuses on several classes of compounds reported to be potential inhibitors of Mpro, such as phenols and polyphenols, alkaloids, and terpenoids.
Article
Agronomy
Sonya Sokornova, Daniil Malygin, Anton Terentev, Viktor Dolzhenko
Summary: Arbuscular mycorrhizal fungi play an important role in the successful distribution of invasive Asteraceae species, although the diversity of their AMF communities is relatively low. Invasive Asteraceae species reduce the richness of native AMF species and also have significant effects on the formation of soil and rhizosphere microbiota.
Article
Food Science & Technology
Elena V. V. Tyutereva, Anna A. A. Dalinova, Kirill N. N. Demchenko, Valeriya A. A. Dmitrieva, Vsevolod R. R. Dubovik, Yuriy V. V. Lukinskiy, Galina V. V. Mitina, Olga V. V. Voitsekhovskaja, Alexander Berestetskiy
Summary: This study investigated the effects of two ten-membered lactones on different plants and found that these toxins caused leaf damage, decreased pigment content, and electrolyte leakage. Additionally, these compounds also affected mitochondrial membrane potential and cell division. The results of this study revealed the mechanisms of action of these phytotoxic macrolides.
Article
Mycology
Vadim Kryukov, Elena Kosman, Oksana Tomilova, Olga Polenogova, Ulyana Rotskaya, Olga Yaroslavtseva, Dilara Salimova, Natalia Kryukova, Alexander Berestetskiy
Summary: Tenuazonic acid (TeA), synthesized by phytopathogenic fungi, is found in a wide range of foods and its toxicity to animals is known, but its mechanisms of action on insects are unclear. In this study, TeA was orally administered to Galleria mellonella larvae, resulting in delayed growth, apoptosis-like changes in midgut cells, and increased midgut bacterial load. Decreased activities of detoxification enzymes and downregulation of certain genes were observed, while other genes and phenoloxidase activity were upregulated. TeA increased susceptibility to Beauveria bassiana but decreased susceptibility to Bacillus thuringiensis. These findings suggest that TeA affects gut physiology, immunity, and systemic responses in wax moth larvae.
MYCOTOXIN RESEARCH
(2023)
Review
Plant Sciences
Alexander Berestetskiy
Summary: Weeds are a permanent component of anthropogenic ecosystems, requiring strict control to avoid seed accumulation in the soil. Excessive use of chemical herbicides has led to contamination and the emergence of herbicide-resistant weeds. Therefore, the development of environmentally friendly herbicides with new mechanisms of action is important.
Review
Biochemistry & Molecular Biology
Vsevolod Dubovik, Anna Dalinova, Alexander Berestetskiy
Summary: Ten-membered lactones (TMLs) are a diverse group of natural polyketides that are abundant in fungi, bacteria, marine organisms, and insects. These compounds exhibit a wide range of biological activities, including phytotoxic, cytotoxic, antifungal, antibacterial, and others. However, there is still a lack of common understanding regarding the natural sources, structural classification, and most characteristic biological activities of TMLs. The problems include controversial nomenclature, incorrect structure elucidation, poor identification of producing organisms, and scattered information on the biological activity of compounds.
NATURAL PRODUCT REPORTS
(2023)
Proceedings Paper
Agricultural Engineering
S. Didovich, A. O. Berestetskiy, E. L. Gasich, A. N. Pas, O. P. Alekseenko
Summary: This study evaluated the use of various microorganisms for controlling the quarantine weed common ragweed, and found that multi-component formulations had higher herbicidal efficacy compared to single microorganism treatments in laboratory conditions. The emulsion formulation containing refined vegetable oil, lecithin, Tween 80, and mycelium of S. heliopsidis exhibited promising results in field conditions, causing significant damage to the weed two weeks post treatment.
INTERNATIONAL CONFERENCE ON PRODUCTION AND PROCESSING OF AGRICULTURAL RAW MATERIALS
(2021)
