Article
Immunology
Guillermo Quindos, Katherine Miranda-Cadena, Rosario San-Millan, Katyna Borroto-Esoda, Emilia Canton, Maria Jose Linares-Sicilia, Axel Hamprecht, Isabel Montesinos, Anna Maria Tortorano, Anna Prigitano, Matxalen Vidal-Garcia, Cristina Marcos-Arias, Andrea Guridi, Ferran Sanchez-Reus, Jesus Machuca-Barcena, Manuel Antonio Rodriguez-Iglesias, Estrella Martin-Mazuelos, Carmen Castro-Mendez, Leyre Lopez-Soria, Alba Ruiz-Gaitan, Marcelo Fernandez-Rivero, Damaris Lorenzo, Javier Capilla, Antonio Rezusta, Javier Peman, Josep Guarro, Joana Pereira, Celia Pais, Orazio Romeo, Guillermo Ezpeleta, Nerea Jauregizar, David Angulo, Elena Eraso
Summary: This study assessed the in vitro activity of Ibrexafungerp and other drugs against 434 blood isolates of Candida in Europe. The results showed that Ibrexafungerp exhibited potent activity against these isolates and also showed activity against fluconazole- and echinocandin-resistant isolates.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Oncology
Maria Szymankiewicz, Krzysztof Kamecki, Sylwia Jarzynka, Anna Koryszewska-Baginska, Gabriela Oledzka, Tomasz Nowikiewicz
Summary: This case study describes the development of echinocandin resistance in a patient with muscle-invasive bladder cancer who underwent radical cystoprostatectomy and was exposed to echinocandins. While resistance to echinocandins is rare, it should be considered in oncology patients with complex treatment regimens.
FRONTIERS IN ONCOLOGY
(2021)
Article
Microbiology
Hazim O. Khalifa, Hidetaka Majima, Akira Watanabe, Katsuhiko Kamei
Summary: This study analyzed the interaction of 21 antifungal combinations with C. glabrata isolates, finding that most caspofungin combinations with azoles or amphotericin B showed synergistic interactions, while azole and 5-flucytosine combinations with azoles or amphotericin B did not. None of the combinations showed antagonistic interactions, with most of the detected synergistic actions occurring among echinocandin-resistant isolates.
Article
Dermatology
Saba Memon, Najia Karim Ghanchi, Urooj Zafar, Joveria Farooqi, Sadaf Zaka, Kauser Jabeen
Summary: A study in Pakistan found low frequencies of both non-synonymous and synonymous polymorphisms in invasive Candida glabrata strains. A novel mutation at codon 661 in the fks2 gene was observed and needs further correlation with treatment outcome data and periodic genomic surveillance.
Review
Immunology
Ahmed Gamal, Sherman Chu, Thomas S. McCormick, Katyna Borroto-Esoda, David Angulo, Mahmoud A. Ghannoum
Summary: Systemic infections caused by Candida glabrata pose a significant threat to both immunocompromised and non-immunocompromised patients, with increasing resistance to traditional antifungal therapies. Ibrexafungerp, a first-in-class glucan synthase inhibitor, shows promising broad-spectrum activity against C. glabrata, including drug-resistant strains, and has potential for treating vulvovaginal candidiasis. Further preclinical and clinical studies are needed to explore its mechanisms and evaluate its efficacy as a new agent for C. glabrata infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Jeremy A. W. Gold, Emma E. Seagle, Joelle Nadle, Devra M. Barter, Christopher A. Czaja, Helen Johnston, Monica M. Farley, Stepy Thomas, Lee H. Harrison, Jill Fischer, Brittany Pattee, Rajal K. Mody, Erin C. Phipps, Sarah Shrum Davis, Brenda L. Tesini, Alexia Y. Zhang, Tiffanie M. Markus, William Schaffner, Shawn R. Lockhart, Snigdha Vallabhaneni, Brendan R. Jackson, Meghan Lyman
Summary: The study found that a considerable proportion of patients with candidemia were initially treated with fluconazole, which may not be appropriate for cases involving non-albicans or fluconazole-resistant species. Therefore, it is important to evaluate the reasons for nonadherence to treatment guidelines and provide clinician education.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Qiqi Wang, Yun Li, Xuan Cai, Ruoyu Li, Bo Zheng, Ence Yang, Tianyu Liang, Xinyu Yang, Zhe Wan, Wei Liu
Summary: In this study, multidrug-resistant isolates of Candida glabrata exhibited resistance through different mechanisms. Overexpression of CDR1 and ERG11 in one isolate, and only overexpression of CDR1 in the other, may mediate resistance to POS. The S663P mutation in FKS2 and up-regulation of FKS2 in both isolates may contribute to echinocandin resistance.
Article
Microbiology
Dipti Sharma, Raees A. Paul, Shivaprakash M. Rudramurthy, Nisha Kashyap, Sanjay Bhattacharya, Rajeev Soman, Shamanth A. Shankarnarayan, Dipali Chavan, Shreya Singh, Parijit Das, Harsimran Kaur, Anup K. Ghosh, Rajendra Prasad, Kaustuv Sanyal, Arunaloke Chakrabarti
Summary: Echinocandins are frontline antifungal agents for the management of invasive infections due to multidrug resistant Candida auris. This study evaluated echinocandin resistance in 199 C. auris isolates from 30 centers across India, identifying FKS1 mutations as key factors. The study found that FKS1 genotype is a more accurate predictor of in vivo response than MIC, and other factors such as chitin content can also influence the response to echinocandins.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Bao Gia Vu, Lucia Simonicova, W. Scott Moye-Rowley
Summary: Fluconazole use leads to the selection of resistant organisms, particularly in Candida glabrata. Elevated minimal inhibitory concentrations for fluconazole in C. glabrata are associated with mutations in the PDR1 gene. Calcineurin, a protein phosphatase, is required for the induction of PDR1 transcriptional regulation by fluconazole, and it links azole and echinocandin susceptibility together through the control of transcription factor activity.
Article
Microbiology
Milena Kordalewska, Geselle Cancino-Prado, Joao Nobrega de Almeida Jr, Igor Brasil Brandao, Renata Tigulini de Souza Peral, Arnaldo. L. L. Colombo, David. S. S. Perlin
Summary: We investigated the echinocandin susceptibility and FKS1 genotypes in clinical isolates of Candida auris from patients in Salvador, Brazil. Three isolates with a novel FKS1 mutation were found to be resistant to echinocandins, causing elevated minimum inhibitory concentrations. Introduction of the Fks1 W691L mutation into susceptible strains also resulted in increased resistance to echinocandins.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Miriam Alisa Knoll, Eldina Samardzic, Wilfried Posch, Cornelia Lass-Floerl
Summary: This study evaluated the influence of inoculum preparation on the detection of resistant and susceptible subpopulations of Candida in EUCAST broth dilution and Etest methods. The results showed that modifying the inoculum preparation method can improve the identification of resistant subpopulations.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Shuying Yu, Padmaja Paderu, Annie Lee, Sami Eirekat, Kelley Healey, Liang Chen, David S. Perlin, Yanan Zhao
Summary: Gcn5 plays a critical role in modulating the virulence and antifungal response of Candida glabrata. Deletion of GCN5 increases sensitivity to stressors and reduces drug resistance, while also impacting intracellular survival in host macrophages.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Zuzana Javorova Rihova, Lubica Slobodova, Anna Hrabovska
Summary: Candiduria is a common nosocomial infection in hospitalized patients that may progress into life-threatening candidemia. Effective treatment of urosepsis requires early and effective antifungal therapy, but the challenge lies in the increasing drug-resistance among Candida spp.
Article
Microbiology
Judith Diaz-Garcia, Aina Mesquida, Ana Gomez, Marina Machado, Pablo Martin-Rabadan, Luis Alcala, Carlos Sanchez-Carrillo, Elena Reigadas, Teresa Vicente, Patricia Munoz, Pilar Escribano, Jesus Guinea
Summary: The study identified compartmentalization of antifungal resistance in Candida isolates, with higher resistance rates observed in abdominal cavity samples, especially in Candida glabrata. Resistance was mainly due to antifungal treatment rather than the spread of resistant clones.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Tatsuro Hirayama, Taiga Miyazaki, Makoto Sumiyoshi, Yuya Ito, Nobuyuki Ashizawa, Kazuaki Takeda, Naoki Iwanaga, Takahiro Takazono, Kazuko Yamamoto, Koichi Izumikawa, Katsunori Yanagihara, Koichi Makimura, Kazuhiro Tsukamoto, Shigeru Kohno, Hiroshi Mukae
Summary: Candida auris is resistant to multiple antifungal agents. This study investigated the antifungal susceptibility of C. auris and explored FKS1 mutations in isolates from mice colonized with wild-type C. auris and treated with echinocandin. Resistant isolates with FKS1 mutations were found to be micafungin- and caspofungin-resistant in vivo, but their minimum inhibitory concentrations (MICs) remained below the micafungin breakpoint in vitro.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
