Journal
ANALYTICA CHIMICA ACTA
Volume 1089, Issue -, Pages 152-164Publisher
ELSEVIER
DOI: 10.1016/j.aca.2019.09.004
Keywords
Chemiluminescence; Insulin; Graphene oxide aerogel; Aptamer; Gold nanoparticles
Categories
Funding
- National Science Foundation for Young Scientists of China [21605094]
- Youth Science fund of Shandong Academy of Sciences [2017QN006]
- Shandong Province Natural Science Institute Joint Fund [ZR2015YL006]
- Shandong Provincial Natural Science Foundation of China [ZR2016BM01]
- Horizontal Scientific Research Project of China [W15077]
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A novel, highly selective and sensitive chemiluminescence (CL) biosensor for insulin (INS) detection was proposed based on aptamer and oligonucleotide-gold nanoparticles functionalized nanosilica @ graphene oxide aerogel. Initially, nanosilica functionalized graphene oxide aerogel (SiO2@GOAG) was successfully prepared and the composite showed rich pore distribution, large specific surface area and good biocompatibility. Insulin aptamer (IGA3) was used as a biorecognition element and oligonucleotide functionalized gold nanoparticles (ssDNA-AuNPs) was used as CL signal amplification materials, which were functionalized on the surface of SiO2@GOAG. The multi-functionalized composite - ssDNA-AuNPs/IGA3/SiO2@ GOAG was obtained and used to construct the CL biosensor for insulin detection. When insulin is present in a sample, the insulin will bind to the IGA3, which will result in the release of ssDNA-AuNPs. The released ssDNA-AuNPs would catalyze the luminescence of luminol and H2O2. The linear range of the CL biosensor for insulin detection was 7.5 x 10(-12) to 5.0 x 10(-9) moL/L and the detection limit was 1.6 x 10(-12) moL/L (S/N = 3). The selectivity and stability of the CL biosensor were also studied and the results showed that the biosensor exhibited high selectivity and good stability due to the introduction of ssDNA-AuNPs/IGA3/SiO2@GOAG. The CL biosensor was finally used for recombinant human insulin detection in recombinant human insulin injection and the results were satisfactory. (C) 2019 Elsevier B.V. All rights reserved.
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