Journal
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
Volume 317, Issue 6, Pages C1183-C1193Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpcell.00012.2019
Keywords
apoptosis; autophagy; cumulus cells; DUSP1; oxidative stress
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Funding
- National Natural Science Foundation of China [31601940, 31572400]
- Modern Agricultural Industry Technology System [CARS-37]
- Science and Technology Project of Jilin Province [20170623035TC, 20170203010NY, SXGJSF2017-6]
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Dual-specificity phosphatase 1 (DUSP1) is differentially expressed in cumulus cells of different physiological states, but its specific function and mechanism of action remain unclear. In this study, we explored the effects of DUSP1 expression inhibition on cell cycle progression, proliferation, apoptosis, and lactate and cholesterol levels in cumulus cells and examined reactive oxygen species levels, mitochondrial function, autophagy, and the expression of key cytokine genes. The results showed that inhibition of DUSP1 in cumulus cells caused abnormal cell cycle progression, increased cell proliferation, decreased apoptosis rates, increased cholesterol synthesis and lactic acid content, and increased cell expansion. The main reason for these effects was that inhibition of DUSP1 reduced ROS accumulation, increased glutathione level and mitochondrial membrane potential, and reduced autophagy levels in cells. These results indicate that DUSP1 limits the biological function of bovine cumulus cells under normal physiological conditions and will greatly contribute to further explorations of the physiological functions of cumulus cells and the interactions of the cumulusoocyte complex.
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