Review
Medicine, Research & Experimental
Trey W. Pittman, Daniel Balazs Decsi, Chamindie Punyadeera, Charles S. Henry
Summary: There has been a growing interest in using microfluidic devices for saliva analysis in recent years. This approach has the potential to provide more sensitive and selective analysis by collecting large quantities of saliva samples. The aim of this review is to provide an update on the use of microfluidic devices for salivary biomarker analysis.
Article
Biophysics
Bruno Gil Rosa, Oluwatomi E. Akingbade, Xiaotong Guo, Laura Gonzalez-Macia, Michael A. Crone, Loren P. Cameron, Paul Freemont, Kwang-Leong Choy, Firat Guder, Eric Yeatman, David J. Sharp, Bing Li
Summary: Accurate and cost-effective multiplexed immunosensors are important for early diagnosis and monitoring of progressive diseases. This review compares different techniques for the development of multiplexed immunosensors and discusses the barriers and potential solutions for translating this strategy into clinical practice.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Chemistry, Analytical
Leyla Karadurmus, S. Irem Kaya, Ahmet Cetinkaya, Sibel A. Ozkan
Summary: Lack of timely detection or proper monitoring of diseases is still a leading cause of death. Developing a portable device similar to a portable laboratory for rapid disease diagnosis is crucial. Electroanalytical methods, particularly using nanomaterials like Mxenes, are cost-effective and highly sensitive for point-of-care diagnostics. This review provides an overview of recent studies on electrochemical POC diagnostics using Mxenes and offers insights into improving selectivity and sensitivity in sensor studies.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Public, Environmental & Occupational Health
Adrian M. Shields, Hannah Brown, Neil Phillips, Mark T. Drayson, Anton A. Richter, Alex G. Richter
Summary: Health care professionals around the world recognize the importance of COVID-19 testing, but many are unsure about the timing of investigations relative to disease symptoms. Confidence in the accuracy of assays varies inversely with COVID-19-related mortality in different countries, but not with the total number of tests performed. There is global consensus that positive antigen and antibody testing gives confidence in returning to work, with saliva being the preferred sampling fluid for COVID-19 diagnostic tests.
FRONTIERS IN PUBLIC HEALTH
(2021)
Review
Chemistry, Analytical
Md Alamgir Kabir, Hussein Zilouchian, Muhammad Awais Younas, Waseem Asghar
Summary: The dengue virus infects millions of people annually, and having access to testing is crucial in preventing future infections. There are various traditional and emerging methods for DENV testing, which can be effective in rural and low-income areas. Future guidelines are needed to enhance the efficiency of diagnostic tools during DENV recurrence or future outbreaks.
Article
Biophysics
Menglin Song, Man-Chung Wong, Lihua Li, Feng Guo, Yuan Liu, Yingjing Ma, Xinyue Lao, Pui Wang, Honglin Chen, Mo Yang, Jianhua Hao
Summary: We have developed a point-of-care upconversion luminescence diagnostics (PULD) method combined with a streamlined smartphone-based portable platform for rapid testing of the SARS-CoV-2 nucleocapsid (N) gene. This method demonstrates high sensitivity and signal-to-noise ratio, and can accurately identify positive COVID-19 samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Immunology
Ilesh Jani, Trevor F. Peter
Summary: Testing programs for SARS-CoV-2 have relied on PCR laboratory tests and rapid antigen assays, but their limitations in cost and accessibility have contributed to inequity in COVID-19 testing. Low-cost point-of-care nucleic acid tests have the potential to address these issues and improve pandemic preparedness, as well as strengthen primary healthcare services.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Medical Laboratory Technology
Gerald J. Kost
Summary: The study used original mathematics to interpret COVID-19 test metrics and compare predictive values for different types of tests. High sensitivity/specificity tests were found to improve predictive values, and multiplex testing can effectively differentiate COVID-19 from influenza/RSV.
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
(2021)
Article
Medicine, General & Internal
Sasa Rajsic, Robert Breitkopf, Mirjam Bachler, Benedikt Treml
Summary: The concept of intensive care units (ICU) has evolved over 70 years, with multidisciplinary care and early diagnostics playing a crucial role in improving patient outcomes and reducing mortality rates. In the current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, there is a particular focus on point-of-care diagnostic techniques to address concerns related to infection transmission and patient safety.
Article
Public, Environmental & Occupational Health
Portia Chipendo, Yusra R. Shawar, Jeremy Shiffman, Junaid Abdul Razzak
Summary: The study identified the challenges faced by global emergency care advocates, including problem definition, coalition building, lack of data, and positioning. Advocates should take advantage of the emerging governance structure, build consensus on definitions, generate data-driven solutions, find strategic framings, and engage with non-traditional allies in order to improve global priority for emergency care.
Article
Agriculture, Dairy & Animal Science
Anais Chancy, Debora E. Santschi, Eric R. Paquet, David L. Renaud, Marie-Lou Gauthier, Edith Charbonneau, Nicolas Barbeau-Gregoire, Laura Van Driessche, Sebastien Buczinski
Summary: The objective of this study was to standardize a reliable and repeatable swabbing technique using ATP luminometry to assess the cleanliness of feeding equipment for preweaning calves. The results showed excellent reliability of the direct swabbing technique and a positive correlation between luminometer values and visual scores and bacterial culture results.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Medicine, General & Internal
Ismo T. Raisanen, Hanna Lahteenmaki, Shipra Gupta, Andreas Grigoriadis, Vaibhav Sahni, Juho Suojanen, Hanna Seppanen, Taina Tervahartiala, Dimitra Sakellari, Timo Sorsa
Summary: This study proposed an efficient strategy based on biomarkers and questionnaires to help medical professionals identify undiagnosed periodontitis patients. The use of oral fluid biomarkers in conjunction with a questionnaire was found to have better diagnostic precision compared to a plain questionnaire.
Article
Chemistry, Analytical
Shihua Li, Nangen Yang, Qiuping Ma, Shijie Li, Shufen Tong, Jiewei Luo, Xiaorong Song, Huanghao Yang
Summary: This study investigates the oxidation responsiveness of near-infrared II (NIR-II) luminescent AuNCs and finds that AuNCs capped with different ligands have different oxidation properties in the presence of peroxynitrite anions (ONOO-). N-acetylcysteine-capped AuNCs are more easily oxidized and aggregate into large-sized assemblies, while macromolecular mercapto-beta-cyclodextrin-capped AuNCs do not show this behavior. Furthermore, N-acetylcysteine-capped AuNCs are demonstrated to be effective for sensitive NIR-II PL imaging of mice acute kidney injury (AKI), thanks to the synergistic effect of in situ AuNC aggregation and decreased glomerular filtration rate (GFR) in the injured kidney, surpassing methods solely based on decreased GFR.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Kimia Zarean Mousaabadi, Zahra Talebi Vandishi, Mansoure Kermani, Nastaran Arab, Ali A. Ensafi
Summary: This review article provides an overview of the application of microfluidics in point-of-care diagnostics, particularly focusing on rapid diagnosis of viral infections. The article introduces the principles of microfluidics, the components of microfluidic devices, and discusses the advantages and disadvantages of microfluidics in diagnostics. It also highlights examples of microfluidic diagnostic devices for viral infections and explores sensing technologies and recent advancements in this field. Additionally, the article describes the materials and fabrication techniques used in microfluidic devices and addresses the challenges in their development as diagnostic sensors.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Public, Environmental & Occupational Health
Eric A. Friedman, Robert Bickford, Charles Bjork, James Campbell, Giorgio Cometto, Alexandra Finch, Catherine Kane, Sarah Wetter, Lawrence Gostin
Summary: During the COVID-19 pandemic, the World Health Assembly requested WHO to develop a global health and care worker compact to protect their rights. A review found that there is a robust legal and policy framework in place, but these commitments are not being fully met.
Article
Chemistry, Analytical
Ayemeh Bagheri Hashkavayi, Byung Seok Cha, Sung Hyun Hwang, Jimin Kim, Ki Soo Park
Summary: An electrochemical aptasensor based on rolling circle amplification (RCA) and the catalytic ability of the hemin/G-quadruplex complex was developed for highly sensitive and selective detection of circulating tumor cells (CTCs). This strategy allows for the sensitive determination of target cancer cells down to a single cell with high selectivity even in human serum. The proposed platform with dual signal amplification shows promise for simple and ultrasensitive detection of CTCs, as well as other cancer cells and biomarkers in different clinical and biological samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Editorial Material
Chemistry, Multidisciplinary
Jinwoo Cheon, Jianhua Qjn, Luke P. Lee, Hakho Lee
ACCOUNTS OF CHEMICAL RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Tugba Kilic, Isabel Gessner, Young Kwan Cho, Naebong Jeong, Jeremy Quintana, Ralph Weissleder, Hakho Lee
Summary: A new polymeric material called zwitterionic polypyrrole (ZiPPy) is reported to have optimal surface condition for biosensing electrodes. ZiPPy combines the advantages of efficient hydration of the electrode surface and rapid deposition through electropolymerization. The ZiPPy-coated electrodes show lower electrochemical impedance and less nonspecific protein adsorption, outperforming other types of electrodes. Moreover, affinity ligands can be immobilized together with ZiPPy in a single-step electropolymerization. The ZiPPy-coated electrodes specifically detect SARS-CoV-2 antibodies in human saliva without the need for sample purification or secondary labeling.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Tugba Kilic, Young Kwan Cho, Naebong Jeong, Ik-Soo Shin, Bob S. Carter, Leonora Balaj, Ralph Weissleder, Hakho Lee
Summary: The article introduces a new method that detects protein markers in extracellular vesicles through impedance spectroscopy, without the need for additional labeling, streamlining the entire detection process. The method is characterized by fast immobilization of antibodies, high sensitivity, and parallel detection, and has shown potential clinical utility.
ACS CENTRAL SCIENCE
(2022)
Article
Chemistry, Physical
Ranjith Thangavel, Daseul Han, Brindha Moorthy, Bala Krishnan Ganesan, Megala Moorthy, Yongll Park, Kyung-Wan Nam, Yun-Sung Lee
Summary: This study reveals some key structural changes of symmetrical sodium-ion batteries during electrochemical reactions, and demonstrates through experiments that symmetrical NVP cells can stably provide high energy capacity.
Article
Chemistry, Physical
Eunyoung Lee, Panju Kim, Byung Joon Moon, Hyunjung Lee, Sang-Wan Ryu, Yong Il Park, Jun-Seok Ha, Sang Hyun Lee
Summary: The combination of inorganic perovskite and graphene exhibits superior optical and electric properties. By directly growing CsPbBr3 nanoplates with graphene oxide (GO) as an additive, blue photoluminescence is achieved. The morphology change of CsPbBr3 from nanocube to nanoplate leads to blue-shifted optical absorption and radiative emission due to quantum size effect. The nanocomposites composed of CsPbBr3 and GO exhibit a shorter lifetime of radiative recombination.
APPLIED SURFACE SCIENCE
(2022)
Article
Engineering, Environmental
Yoongu Lim, Song Yeul Lee, Dohun Kim, Mi-Kyung Han, Hyun Soo Han, Soon Hyung Kang, Jung Kyu Kim, Uk Sim, Yong Il Park
Summary: The key to increasing photocatalytic activity is to maximize solar spectrum utilization and minimize the recombination of photosynthetic carriers. In this study, a novel heterojunction photoelectrode utilizing upconversion nanoparticles (UCNPs) and ZnFe2O4 was designed to improve solar absorption efficiency. The combination of UCNPs and ZFO demonstrated a significant enhancement in photoelectrochemical performance.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Physical
Song Yeul Lee, Joo-Yeon Park, Hyun-Jae Kim, Yun-Sung Lee, Yong Il Park
Summary: In this study, a Prussian blue-graphene oxide composite (PBGO) was developed as a battery-type cathode for sodium-ion capacitors (SIC). PBGO exhibited high capacity, high energy density, and excellent cyclic stability, along with a superior capacity retention.
JOURNAL OF ALLOYS AND COMPOUNDS
(2022)
Article
Chemistry, Physical
Song Yeul Lee, Dasom Park, Byung Sun Yoon, Yun-Sung Lee, Yong Il Park, Chang Hyun Ko
Summary: This study demonstrates that atomic layer deposition (ALD) can be used to deposit thin films on powdered substrates, providing potential applications for powder-type nanoparticles.
JOURNAL OF ALLOYS AND COMPOUNDS
(2022)
Article
Chemistry, Multidisciplinary
Jin-Ho Park, Eung-Kyu Park, Young Kwan Cho, Ik-Soo Shin, Hakho Lee
Summary: This study describes a method that transforms ubiquitous smartphones into robust lateral flow assay readers by utilizing optical calibration. The method corrects shape distortion, illumination brightness, and color imbalances through image processing, enabling consistent optical signals for quantitative molecular analysis under different illumination conditions.
Article
Chemistry, Multidisciplinary
V Naresh, V. N. K. B. Adusumalli, Y. Park, N. Lee
Summary: This study demonstrates the tunable up-conversion luminescence properties of CsPb(Br1-x/I-x)(3) perovskite nanocrystals (PeNCs) sensitized by NaYF4:Yb,Tm@NaYF4 up-conversion nanoparticles (UCNPs) at 980 nm excitation. By adjusting the UCNPs/PeNCs concentration ratio, UC white light emission is achieved, and a prototype of a UC white light-emitting diode is developed. Furthermore, a dual-model strategy based on UC and down-conversion luminescence is utilized for anti-counterfeiting, improving the security and solving the issue of photostability.
MATERIALS TODAY CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yoon Ho Roh, Chang Yeol Lee, Sujin Lee, Hyunho Kim, Amy Ly, Cesar M. Castro, Jinwoo Cheon, Jae-Hyun Lee, Hakho Lee
Summary: CRISPR/Cas systems can translate DNA sequence information into amplified signals, but multiplexed assays are challenging. This article describes a hydrogel-based CRISPR/Cas12 system called CLAMP, which compartmentalizes the reaction in spatially-encoded hydrogel microparticles. Each microparticle has a unique face code and becomes fluorescent in the presence of target DNA. The assay is simplified by capturing the microparticles in a microfluidic device and automatically recognizing them using a machine learning algorithm. CLAMP assay is fast, highly sensitive (attomolar detection limit with preamplification), and capable of multiplexing in a single experiment. As a proof-of-concept clinical application, CLAMP is used to detect nucleic acid targets of human papillomavirus in cervical brushing samples.
Article
Biochemistry & Molecular Biology
Young Jun Jang, Byung Seok Cha, Doyeon Kim, Eun Sung Lee, Seokjoon Kim, Jinjoo Han, Jiye Shin, Seokhwan Kim, Ki Soo Park
Summary: This study proposes a new strategy to enhance the biological activities of astaxanthin (AST) using fetal bovine serum-derived extracellular vesicles (EVs). AST-incorporated EVs (EV-ASTs) showed high stability and maintained their original EV morphology, resulting in excellent antioxidant and anti-inflammatory activities in various cells. The mechanism behind the enhanced biological activities of EV-ASTs was also investigated. These findings provide insights into improving the performance of hydrophobic drugs using nature-derived EVs and contribute to the development of novel drug-delivery systems.
Article
Chemistry, Analytical
Daehan Nam, Seokjoon Kim, Jung Ho Kim, Seungjin Lee, Daneub Kim, Jinseo Son, Doyeon Kim, Byung Seok Cha, Eun Sung Lee, Ki Soo Park
Summary: Loop-mediated isothermal amplification (LAMP) is a widely used molecular diagnostics technology. This study introduces a new version of LAMP, called low-temperature LAMP, which operates at 37 degrees C for on-site diagnosis. The low-temperature LAMP differs from conventional LAMP in terms of MgSO4 and dNTPs concentrations, as well as the lengths of DNA probes. Under optimal conditions, low-temperature LAMP shows comparable amplification efficiency to conventional LAMP. Additionally, the method enables the detection of miR-21 with high sensitivity and selectivity at 37 degrees C, without requiring expensive DNA modifications or special additives.
Article
Engineering, Chemical
Yong Il Park
Summary: Fluorescence imaging is widely used in cell and small animal imaging due to its high temporal and spatial resolution. However, conventional fluorescence imaging using visible light has limitations such as low penetration depth, phototoxicity, and interference from background autofluorescence. To overcome these limitations, fluorescence imaging using near-infrared-I (NIR-I) and near-infrared-II (NIR-II) light has been developed. NIR-II imaging offers advantages of reduced light scattering, improved penetration depth, and reduced autofluorescence. Inorganic nanoparticle-based probes, including single-walled carbon nanotubes, quantum dots, and lanthanide nanoparticles, have been explored for NIR-II fluorescence imaging due to their excellent photostability and tunable emission wavelength.
APPLIED CHEMISTRY FOR ENGINEERING
(2022)
