Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 11, Issue 38, Pages 34621-34633Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b10074
Keywords
DNA; nanosilicate; dual-cross-linked; sustained release; stem cell recruitment; regenerative medicine
Funding
- University of Kansas
- Institutional Development Award (IDeA) from the National Institute of General Medical Sciences (NIGMS) of the NIH [P20GM103638]
- Umbilical Cord Matrix Project fund from the State of Kansas
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In situ tissue repair holds great potential as a cell-free regenerative strategy. A critical aspect of this approach is the selection of cell instructive materials that can efficiently regulate the defect microenvironment via the release of chemoattractant factors to mobilize and recruit endogenous stem cells toward the site of implantation. Here we report the design of a DNA-based hydrogel as a drug delivery platform for the sustained release of a promising chemoattractant, SDF-1 alpha. The hydrogel is composed of chemically cross-linked DNA strands, which are bridged via silicate nanodisks (nSi). Silicate nanodisks electrostatically interact with the negatively charged DNA backbone resulting in the formation of a dual cross-linked nanocomposite hydrogel with a combination of chemical and physical cross-link points. The formulated nanocomposites display enhanced elasticity and mechanical toughness as compared to their nonsilicate containing counterparts. Moreover, the electrostatic interaction between nSi and SDF-1 alpha leads to sustained release of the chemokine from the hydrogels. The in vitro bioactivity assays confirm the retention of chemotactic properties of the protein after its release. Overall, the dual cross-linked DNA-based hydrogel platform could be potentially used as a cell-instructive material for the recruitment of host stem cells to guide the process of in situ tissue repair.
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