Review
Oncology
Barbora Kvokackova, Jan Remsik, Mohit Kumar Jolly, Karel Soucek
Summary: Epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) play critical roles in the development of TNBC, shaping aggressive and heterogeneous biological features, but may also contribute to the progression of metastatic disease.
Article
Chemistry, Physical
Xiang Xiao, Xinxing Miao, Shanzhou Duan, Sidi Liu, Qinghua Cao, Renfei Wu, Chengcheng Tao, Jian Zhao, Qing Qu, Aleksandra Markiewicz, Rui Peng, Yongbing Chen, Anna Zaczek, Jian Liu
Summary: An integrated platform of single-cell fluorescent enzymatic assays with superwetting droplet-array microchips (SDAM) is reported, enabling ultrasensitive functional screening of epithelial-mesenchymal sub-phenotypes of circulating tumor cells (CTCs). The SDAM generates high-density, volume well-defined droplet arrays that can isolate single tumor cells via a discontinuous dewetting effect. It allows for sensitive detection of MMP9 enzyme activities secreted by single tumor cells, which correlates with their epithelial-mesenchymal sub-phenotypes.
Article
Biochemical Research Methods
Ayalur Raghu Subbalakshmi, Sarthak Sahoo, Prakruthi Manjunatha, Shaurya Goyal, Vignesh A. Kasiviswanathan, Yeshwanth Mahesh, Soundharya Ramu, Isabelle McMullen, Jason A. A. Somarelli, Mohit Kumar Jolly
Summary: ELF3 is a factor strongly associated with an epithelial phenotype and is inhibited during EMT. It inhibits the progression of EMT and can counteract EMT induction even in the presence of EMT-inducing factors. The prognostic capacity of ELF3 is specific to cell-of-origin or lineage.
JOURNAL OF BIOLOGICAL ENGINEERING
(2023)
Article
Oncology
Ayalur Raghu Subbalakshmi, Sarthak Sahoo, Isabelle McMullen, Aaditya Narayan Saxena, Sudhanva Kalasapura Venugopal, Jason A. Somarelli, Mohit Kumar Jolly
Summary: Research shows that the transcription factor KLF4 can delay the onset of EMT by suppressing multiple EMT-TFs and promote a phenotypic shift towards a more epithelial state. KLF4 can also strengthen its influence on modulating EMT dynamics by inhibiting cell-state transitions at the epigenetic level.
Article
Medicine, General & Internal
Marcin Oplawski, Robert Nowakowski, Agata Srednicka, Dominika Ochnik, Beniamin Oskar Grabarek, Dariusz Boron
Summary: Modern diagnostics focus on molecular analysis and the search for new molecular markers. This study evaluated the differences in mRNA and miRNA expression related to endometrial cancer grades, aiming to select promising molecular markers. The analysis identified potential diagnostic markers associated with epithelial-mesenchymal transition.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Oncology
Sarah M. Groves, Nicholas Panchy, Darren R. Tyson, Leonard A. Harris, Vito Quaranta, Tian Hong
Summary: This study analyzed the relationship between SCLC subtypes and epithelial to mesenchymal transition (EMT), and found a correspondence between SCLC subtypes and the EMT program. This study provides a basis for further understanding the gene regulatory mechanisms of SCLC tumor plasticity and its applicability to other cancer types.
Article
Agriculture, Dairy & Animal Science
Alessandro Sammarco, Chiara Gomiero, Giorgia Beffagna, Laura Cavicchioli, Silvia Ferro, Silvia Michieletto, Enrico Orvieto, Marco Patruno, Valentina Zappulli
Summary: This study investigated the expression of molecules related to epithelial-to-mesenchymal transition (EMT) in human breast cancer, canine mammary tumors, and feline mammary tumors at the gene and protein level. It was found that some typical EMT markers were not highly expressed at the mRNA level in tumors, suggesting the need for investigation of other markers. However, at the protein level, molecules such as vimentin and E-cadherin were associated with higher aggressiveness and potential usefulness as markers. The study also confirmed similarities between feline mammary tumors and triple negative breast cancer, as well as between canine mammary tumors and the less aggressive subtype of human breast cancer that expresses hormonal receptors.
Article
Oncology
Fatima-Zohra Khadri, Marianne Samir Makboul Issac, Louis Arthur Gaboury
Summary: The inflammatory infiltrate in breast cancer is often associated with poorly differentiated and triple-negative carcinomas. Changes in EMT-related markers impact the intensity of inflammatory infiltrate in the TME, showing a potential beneficial association with the immune inflammatory response. Understanding the impact of EMT signature on immune response could lead to new therapeutic approaches in subsets of breast cancer patients.
Article
Biochemical Research Methods
Yingying Ma, Songbiao Zhu, Meiqi Yi, Wenhao Zhang, Yuanyuan Xue, Xiaohui Liu, Haiteng Deng
Summary: Protein S-glutathionylation is an important post-translational modification that regulates cellular processes. This study investigated the changes in glutathionylome during epithelial-mesenchymal transition (EMT) and found that the glutathionylation level of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) increased, inhibiting its enzymatic activity and promoting EMT.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Ophthalmology
Srinivasa R. Sripathi, Ming-Wen Hu, Melissa M. Liu, Jun Wan, Jie Cheng, Yukan Duan, Joseph L. Mertz, Karl J. Wahlin, Julien Maruotti, Cynthia A. Berlinicke, Jiang Qian, Donald J. Zack
Summary: The study induced EMT in human stem cell-derived RPE monolayers using two model systems and found that dysregulated proteocollagenolytic enzymatic dissociation and co-treatment with TGF-beta and TNF-alpha were key factors in promoting EMT. It also identified widespread dysregulation of axon guidance molecules during RPE-EMT progression, suggesting potential therapeutic targets for retinal diseases.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Paras Jain, Sophia Corbo, Kulsoom Mohammad, Sarthak Sahoo, Santhalakshmi Ranganathan, Jason T. George, Herbert Levine, Joseph Taube, Michael Toneff, Mohit Kumar Jolly
Summary: Epithelial-mesenchymal transition (EMT) and its reverse mesenchymal-epithelial transition (MET) play crucial roles in embryonic development, wound healing, and cancer metastasis. While short-term EMT induction can lead to reversible phenotypic changes, long-term EMT induction is often associated with irreversibility. In this study, we demonstrate that the phenotypic changes observed in MCF10A cells during long-term EMT induction by TGF-beta can in fact have longer timescales of reversibility. We also propose a mathematical model that explains how the epigenetic memory gained during long-term EMT induction can slow down the recovery to the epithelial state after TGF-beta withdrawal.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2023)
Article
Multidisciplinary Sciences
Michela Serresi, Sonia Kertalli, Lifei Li, Matthias Jurgen Schmitt, Yuliia Dramaretska, Jikke Wierikx, Danielle Hulsman, Gaetano Gargiulo
Summary: Research has shown that chromatin regulators have a broader impact on EMT interconversion in lung cancer cells than kinases, and the loss of ARID1A, DOT1L, BRD2, and ZMYND8 may have nondeterministic and sometimes opposite consequences. These findings reveal general principles underlying transcriptional control of cancer cell plasticity.
Article
Oncology
Ana Pavlic, Kristian Urh, Katarina Stajer, Emanuela Bostjancic, Nina Zidar
Summary: The study analyzed the expression of the miR-200 family as EMT markers and their target genes in colorectal carcinoma. It found partial EMT at the invasive tumor front and partial MET in metastases based on miR-200 family and its target genes expression.
FRONTIERS IN ONCOLOGY
(2021)
Article
Immunology
Sarthak Sahoo, Sonali Priyadarshini Nayak, Kishore Hari, Prithu Purkait, Susmita Mandal, Akash Kishore, Herbert Levine, Mohit Kumar Jolly
Summary: Recent research shows that cells with hybrid epithelial/mesenchymal characteristics can have high levels of PD-L1, important for immune evasion, without needing to fully undergo epithelial-mesenchymal transition. This cellular state in breast cancer is associated with resistance to anti-estrogen therapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Wen Jia, Mohit Kumar Jolly, Herbert Levine
Summary: The epithelial-mesenchymal transition (EMT) is a crucial cellular process for wound healing, cancer metastasis, and embryonic development. Recent studies have shown that hybrid epithelial/mesenchymal states, possessing both epithelial and mesenchymal traits, play a significant role in cancer metastasis and resistance to therapies. NRF2 has been identified as a stabilizing factor for these hybrid states. This research incorporates a phenomenological epigenetic feedback effect into a computational model for EMT signaling and demonstrates its stabilizing effect on the hybrid state if NRF2 influences SNAIL at an epigenetic level.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Mahesh Agarwal, Amaresh Kumar Sahoo, Biplab Bose
MOLECULAR PHARMACEUTICS
(2017)
Article
Biochemistry & Molecular Biology
Rupamoni Thakur, Ashok Kumar, Biplab Bose, Dulal Panda, Debashree Saikia, Pronobesh Chattopadhyay, Ashis K. Mukherjee
Article
Oncology
Asim Bikas Das, Pojul Loying, Biplab Bose
Article
Medicine, Research & Experimental
Ashok Kumar, Gopal Das, Biplab Bose
MOLECULAR PHARMACEUTICS
(2014)
Article
Multidisciplinary Sciences
Pojul Loying, Janvie Manhas, Sudip Sen, Biplab Bose
Review
Biochemistry & Molecular Biology
Biplab Bose
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
(2013)
Article
Pharmacology & Pharmacy
Mahesh Agarwal, Tanmay Mondal, Biplab Bose
Review
Multidisciplinary Sciences
Vimalathithan Devaraj, Biplab Bose
JOURNAL OF THE INDIAN INSTITUTE OF SCIENCE
(2020)
Article
Multidisciplinary Sciences
Vimalathithan Devaraj, Biplab Bose
Article
Physics, Fluids & Plasmas
Kamleswar Chandrasekaran, Biplab Bose
Article
Biochemistry & Molecular Biology
B Bose, N Khanna, SK Acharya, S Sinha
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2006)