Journal
SCIENCE ADVANCES
Volume 5, Issue 7, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.aaw6441
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Funding
- National Institute of Allergy and Infectious Diseases of the National Institutes of Health (NIH) [R21 AI126037, U54 AI065359]
- Bay Area Lyme Foundation
- institutional funds of UC Irvine
- University of South Carolina
- Office of the Assistant Secretary of Defense for Health Affairs through Tick Borne Disease Research Program [W81XWH-17-1-0481]
- Alfred P. Sloan Foundation
- Genomics High Throughput Facility Shared Resource of the Cancer Center Support Grant [P30CA-062203]
- NIH shared instrumentation grant [1S10RR025496-01, 1S10OD010794-01, 1S10OD021718-01]
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The rodent Peromyscus leucopus is the natural reservoir of several tick-borne infections, including Lyme disease. To expand the knowledge base for this key species in life cycles of several pathogens, we assembled and scaffolded the P. leucopus genome. The resulting assembly was 2.45 Gb in total length, with 24 chromosome-length scaffolds harboring 97% of predicted genes. RNA sequencing following infection of P. leucopus with Borreliella burgdorferi, a Lyme disease agent, shows that, unlike blood, the skin is actively responding to the infection after several weeks. P. leucopus has a high level of segregating nucleotide variation, suggesting that natural resistance alleles to Crispr gene targeting constructs are likely segregating in wild populations. The reference genome will allow for experiments aimed at elucidating the mechanisms by which this widely distributed rodent serves as natural reservoir for several infectious diseases of public health importance, potentially enabling intervention strategies.
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