Article
Immunology
Alma Reyna Escalona-Montano, Mariana Zuniga-Fabian, Nallely Cabrera, Ricardo Mondragon-Flores, Jenny Nancy Gomez-Sandoval, Araceli Rojas-Bernabe, Augusto Gonzalez-Canto, Laila Gutierrez-Kobeh, Ruy Perez-Montfort, Ingeborg Becker, Maria Magdalena Aguirre-Garcia
Summary: In this study, the researchers cloned and characterized the structure and function of PP2C in Leishmania, finding that LmxPP2C exhibited protein dephosphorylation activity under specific conditions. Immunodetection showed its localization in the flagellar structures of the protozoan, suggesting a potentially important role in specific cellular processes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Jianfang Qiu, Lei Ni, Xue Xia, Shihao Chen, Yan Zhang, Min Lang, Mengyu Li, Binman Liu, Yu Pan, Jinhua Li, Xingguo Zhang
Summary: This study conducted a comprehensive investigation of the SlPP2C gene family in tomato and found that these genes play important roles in tomato development, stress response, and phytohormone signaling. The study also revealed that gene duplication was a major factor in the expansion of SlPP2Cs, and confirmed their diverse expression patterns in different tomato tissues.
Article
Biochemistry & Molecular Biology
Yun Chen, Jing-Bo Zhang, Ning Wei, Zhi-Hao Liu, Yang Li, Yong Zheng, Xue-Bao Li
Summary: GhDRP1 acts as a negative regulator in response to drought stress by modulating ABA signaling pathway and flavonoid biosynthesis pathway, affecting stomata movement, water loss, ROS scavenging enzymes, and proline accumulation in cotton. GhDRP1 overexpression transgenic cotton and Arabidopsis exhibited reduced drought tolerance, while GhDRP1-silenced cotton showed enhanced drought tolerance.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Plant Sciences
Xing Huang, Yongsheng Liang, Ronghua Zhang, Baoqing Zhang, Xiupeng Song, Junxian Liu, Manman Lu, Zhenqiang Qin, Dewei Li, Song Li, Yangrui Li
Summary: This study provides a comprehensive analysis of the PP2C gene family in wild sugarcane, revealing 145 PP2C proteins categorized into 13 clades. The phylogenetic analysis showed that SsPP2Cs are evolutionarily closer to sorghum and are the most abundant in number. Furthermore, half of the SsPP2C proteins were found to be located in the chloroplast, indicating their potential role in photosynthesis and stress response.
Article
Plant Sciences
Zhanwei Wu, Lu Luo, Yongshan Wan, Fengzhen Liu
Summary: Plant protein phosphatase 2C (PP2C) plays important roles in response to salt stress by influencing metabolic processes, hormone levels, and growth factors. In this study, 178 PP2C genes were identified in peanut, with segmental duplication in 78 gene pairs. Phylogenetic analysis divided AhPP2Cs into 10 clades (A-J). miRNA analysis identified 22 miRNAs targeting 57 AhPP2C genes. Gene structure and motif analysis revealed similarities in subclades AI and AII. Promoter analysis found MYB binding factors and ABA response elements in six genes. GO and KEGG enrichment analysis confirmed the importance of AhPP2C-A genes in protein binding, signal transduction, and response to abiotic stimulus. Tissue-specific expression of clade A AhPP2Cs was observed, with AhPP2C45 and AhPP2C134 up-regulated in response to salt stress. These findings suggest AhPP2C45 and AhPP2C134 as candidate PP2Cs conferring salt tolerance in peanut breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Hui Ma, Juncheng Lin, Fangming Mei, Hude Mao, Qingshun Q. Li
Summary: The expression of homoeologous genes in allohexaploid wheat genome remains largely unknown at the co-transcriptional level. This study focused on alternative polyadenylation (APA) and found that drought stress led to extensive APA in wheat, involving a large number of stress-responsive genes. The study also revealed different poly(A) sites, APA patterns, and 3' UTR length changes among homoeologous genes. These findings shed light on the role of APA in drought stress response and provide new insights into the adaptability of homoeologous genes through transcriptome diversity.
Article
Biochemistry & Molecular Biology
Yu Chen, Haohan Zhao, Yue Wang, Xiaojun Qiu, Gang Gao, Aiguo Zhu, Ping Chen, Xiaofei Wang, Kunmei Chen, Jia Chen, Peng Chen, Jikang Chen
Summary: In this study, 63 BnPP2C genes were identified and classified in the ramie genome. The study revealed that BnPP2C1 and BnPP2C26 could be candidate genes for drought and salt tolerance in ramie. These findings are important for further understanding the function of PP2C genes and the stress response mechanism in ramie.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Xiaohong Li, Xiaotong Wang, Xuxia Ma, Wenqi Cai, Yaling Liu, Wenxue Song, Bingzhe Fu, Shuxia Li
Summary: In this study, the OSCA gene family in alfalfa was systematically analyzed, and a total of 14 genes were identified and classified into three groups. The results showed that these genes play important roles in response to abiotic and biotic stresses, as well as hormone treatments, providing candidate genes for stress resistance breeding in alfalfa.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Yong-Lu Wei, Jian-Peng Jin, Di Liang, Jie Gao, Jie Li, Qi Xie, Chu-Qiao Lu, Feng-Xi Yang, Gen-Fa Zhu
Summary: We conducted a genome-wide investigation and functional analysis of WRKY transcription factors in Cymbidium sinense. We found that some of the genes in this family were strongly responsive to plant hormones and enhanced plant tolerance to abiotic stress within the ABA-dependent pathway.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Xinjie Shen, Hong Nan, Yuzhuang Jiang, Yujia Zhou, Xuejun Pan
Summary: As a crucial oil crop species, soybean plays a key role in supplying edible oil, plant protein, and animal fodder worldwide. Studies have found that the GmSnRK2s and GmPP2C-As genes play important roles in response to drought stress, including their expression patterns, functions, and interactions with the abscisic acid (ABA) signal.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xue Feng, Qian Yu, Jianbin Zeng, Xiaoyan He, Wenxing Liu
Summary: In this study, 79 GATA genes were identified in wheat and classified into four subfamilies. The analysis of amino acid sequences and expression patterns revealed significant differences among these genes. Furthermore, comparisons with other plant genomes provided insights into the evolutionary characteristics. These findings are important for future gene function analysis, molecular breeding, and stress response research in wheat.
Article
Biochemistry & Molecular Biology
Shuqing Liu, Chao Lei, Zhanhua Zhu, Mingzhen Li, Zhaopeng Chen, Wei He, Bin Liu, Liuping Chen, Xuejun Li, Yanzhou Xie
Summary: This study identified 12 ACS gene family members in wheat. These genes play an important role in regulating wheat growth and responding to adversity stresses. The expression analysis showed that most of the ACS genes had highest expression in roots, suggesting their important role in root development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chuanshun Li, Hengyang Zhang, Yuting Qi, Yaoyao Zhao, Chonghao Duan, Yujiao Wang, Zhe Meng, Quan Zhang
Summary: This study identified and classified 14 PYR/PYL genes, 9 PP2C (A) genes, and 10 SnRK2 genes from the halophytic plant Eutrema. The phylogenetic analysis and gene structure analysis supported the existence of 4 EsPYR/PYL, 4 EsPP2C (A), and 3 EsSnRK2 subfamilies. Furthermore, it was demonstrated that a large-scale segmental duplication event played a major role in the expansion of these gene families. Comparative synteny analysis revealed more orthologous gene pairs between Eutrema and Brassica than between Eutrema and Arabidopsis. RNA-seq and qRT-PCR experiments confirmed the up-regulated expression of several genes in response to ABA, NaCl, or cold stress. Additionally, WGCNA analysis uncovered several co-expression modules containing the PYL-PP2C (A)-SnRK2 genes, providing new insights into their potential molecular functions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Plant Sciences
Bin Linghu, Zhuoling Xu, Yingqi Chu, Ying Yan, Xiaojun Nie, Song Weining
Summary: Calcium (Ca2+)-dependent protein kinase (CDPK) is a biomacromolecule that acts as a unique Ca2+ sensor and effector for calcium signaling, playing a vital role in plant growth, development, and stress responses. This study identified and characterized the CDPK family in wheat, discovering their gene structure, protein domains, conserved motifs, and cis-acting elements. The study also identified tissue-specific and stress-responsive CDPK candidates and demonstrated their roles in drought tolerance. Additionally, the study showed the positive regulation of a specific CDPK isoform by TaDREB3 in improving drought tolerance. This study provides insights into the evolution and function of the CDPK family in wheat.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Zhibin Zhang, Ruili Lv, Bin Wang, Hongwei Xun, Bao Liu, Chunming Xu
Summary: Allopolyploidy is a powerful genetic and evolutionary force that combines divergent genomes into a common nucleus. One of its striking features is the ability to generate rapid and saltational biological consequences through 'genome shock'. In this study, we analyzed the changes in genome-wide gene expression under diverse environmental conditions in a synthetic allotetraploid wheat, revealing that allopolyploidy caused large-scale changes in gene expression that were variable across conditions and exacerbated by stresses and chromosomal exchanges. Our results provide new insights into the direct impacts of allopolyploidy on condition-dependent changes in subgenome expression.
Article
Genetics & Heredity
Peipei Su, Cai Jiang, Hao Qin, Rui Hu, Jialu Feng, Junli Chang, Guangxiao Yang, Guangyuan He
Article
Biotechnology & Applied Microbiology
Jie Xiao, Rui Hu, Ting Gu, Jiapeng Han, Ding Qiu, Peipei Su, Jialu Feng, Junli Chang, Guangxiao Yang, Guangyuan He
Article
Plant Sciences
Qiong Wang, Yin Li, Fusheng Sun, Xiaoyan Li, Pandi Wang, Junli Chang, Yuesheng Wang, Guangxiao Yang, Guangyuan He
Article
Biotechnology & Applied Microbiology
Ruibin Wang, Jingfei Ma, Qian Zhang, Chunlai Wu, Hongyan Zhao, Yanan Wu, Guangxiao Yang, Guangyuan He
Article
Biochemistry & Molecular Biology
Xiaoyan Li, Yin Li, Xiaofen Yu, Fusheng Sun, Guangxiao Yang, Guangyuan He
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Plant Sciences
Xiaofen Yu, Jiapeng Han, Li Li, Qian Zhang, Guangxiao Yang, Guangyuan He
PLANT CELL REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Li Li, Fu Shi, Yaqiong Wang, Xiaofen Yu, Jingjing Zhi, Yanbin Guan, Hongyan Zhao, Junli Chang, Mingjie Chen, Guangxiao Yang, Yuesheng Wang, Guangyuan He
Article
Biochemistry & Molecular Biology
Rui Hu, Jie Xiao, Qian Zhang, Ting Gu, Junli Chang, Guangxiao Yang, Guangyuan He
Article
Biotechnology & Applied Microbiology
Ding Qiu, Wei Hu, Yu Zhou, Jie Xiao, Rui Hu, Qiuhui Wei, Yang Zhang, Jialu Feng, Fusheng Sun, Jiutong Sun, Guangxiao Yang, Guangyuan He
Summary: The study found that TaASR1-D can increase wheat tolerance to multiple abiotic stresses and improve yield under salt stress. This gene enhances abiotic stress tolerance by reinforcing antioxidant capacity and ABA signaling.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Li Li, Fu Shi, Guoli Wang, Yanbin Guan, Yufan Zhang, Mingjie Chen, Junli Chang, Guangxiao Yang, Guangyuan He, Yuesheng Wang, Yin Li
Summary: The present study utilized the known functions of rice SPL genes to study the functional genomics of TaSPL genes in wheat. Through various methods, the orthologous relationships between TaSPL and OsSPL genes were established, highlighting the conservation and divergence in SPL functions. TaSPL genes responsive to different abiotic stresses were identified, and ectopic expression of TaSPL3 in rice had significant effects on heading dates, leaf and stem development, and yield.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Qingchen Luo, Jialu Feng, Guangxiao Yang, Guangyuan He
Summary: This study sheds light on the importance of potassium (K+) in plant growth and identifies a potential regulator, BdCIPK31, which enhances plant tolerance to low potassium stress and promotes potassium uptake and root growth. Additionally, BdCIPK31 mitigates oxidative damage caused by low potassium stress.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Yaqiong Wang, Jian Zeng, Peipei Su, Hongyan Zhao, Li Li, Xiaoxue Xie, Qian Zhang, Ya'nan Wu, Ruibin Wang, Yufan Zhang, Boju Yu, Mingjie Chen, Yuesheng Wang, Guangxiao Yang, Guangyuan He, Junli Chang, Yin Li
Summary: This article provides a detailed protocol and experience for producing transgenic wheat using particle bombardment-mediated method, highlighting its advantages in genotype dependency and vector construction. The method has been successfully applied to multiple wheat genotypes and shows great potential and wide applications.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Jiapeng Han, Xiaoxue Xie, Yang Zhang, Xiaofen Yu, Guangyuan He, Yin Li, Guangxiao Yang
Summary: This study constructs the evolutionary history of the DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN (DREB) subfamily in green plants, revealing its origin, expansion, and adaptive evolution in the process of land plant evolution. The study also identifies a Poaceae-specific gene, ERF014, which shows co-evolution with a small heat shock protein cluster and neofunctionalization driven by heat acclimation.