Article
Cell Biology
Ebony N. Gary, Nicholas J. Tursi, Bryce Warner, Elizabeth M. Parzych, Ali R. Ali, Drew Frase, Estella Moffat, Carissa Embury-Hyatt, Trevor R. F. Smith, Kate E. Broderick, Laurent Humeau, Darwyn Kobasa, Ami Patel, Daniel W. Kulp, David B. Weiner
Summary: By using mucosal chemokines as adjuvants, the efficacy of vaccines can be enhanced on mucosal surfaces, providing significant protection against challenges from Delta variants.
CELL REPORTS MEDICINE
(2022)
Article
Immunology
Richard I. Walker, A. Louis Bourgeois
Summary: Oral immunization is an effective strategy against mucosal enteric pathogens. Inactivated whole bacterial cells can also induce protective immunity. Developing a safe and simple delivery format with controlled immunogenic amounts of complex antigens is essential. Enhancing the efficacy of whole cell vaccines can be achieved through genetic engineering and the use of mucosal adjuvants. The development of ETVAX, a vaccine candidate against enterotoxigenic Escherichia coli (ETEC), has shown promising results.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Baldeep Singh, Isha Pahuja, Priyanka Yadav, Aishwarya Shaji, Shivam Chaturvedi, Anand Ranganathan, Ved Prakash Dwivedi, Gobardhan Das
Summary: All-trans-retinoic acid (ATRA) improves Mycobacterium tuberculosis clearance by modulating MEK/ERK and p38 MAPK pathways, inducing immune responses, and protecting the host from infection. The combination therapy of ATRA and isoniazid (INH) is of great significance for tuberculosis treatment.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Medicine, Research & Experimental
Olivia Amanda Oest Mullertz, Peter Andersen, Dennis Christensen, Camilla Foged, Aneesh Thakur
Summary: The mucosal surfaces of the lungs are a major site for entry of airborne pathogens and pulmonary vaccination can induce protective mucosal immunity. Researchers found that altering the surface charge of liposomes can enhance specific T-cell responses, but the impact on mucosal immunity remains unclear.
MOLECULAR PHARMACEUTICS
(2022)
Article
Microbiology
Gregory H. Babunovic, Michael A. DeJesus, Barbara Bosch, Michael R. Chase, Thibault Barbier, Amy K. Dickey, Bryan D. Bryson, Jeremy M. Rock, Sarah M. Fortune
Summary: The study found that the application of all-trans-retinoic acid (ATRA) can enhance the control of Mycobacterium tuberculosis (Mtb) by human macrophages, which is achieved by altering macrophage cholesterol trafficking and lipid metabolism. In addition, CRISPR interference screening was used to identify specific genes required for Mtb survival in ATRA-activated macrophages.
Article
Microbiology
Gregory H. Babunovic, Michael A. DeJesus, Barbara Bosch, Michael R. Chase, Thibault Barbier, Amy K. Dickey, Bryan D. Bryson, Jeremy M. Rock, Sarah M. Fortune
Summary: This study identifies that the treatment with ATRA can enhance the control ability of macrophages against Mycobacterium tuberculosis (Mtb) by affecting bacterial clearance through changes in macrophage cholesterol metabolism, and conducts the first Mtb CRISPR interference screen in the infection model, revealing certain Mtb genes specifically required to survive in ATRA-activated macrophages.
Review
Nanoscience & Nanotechnology
Behnaz Eshaghi, Alex Schudel, Ilin Sadeghi, Ziqi Chen, Amy H. Lee, Maria Kanelli, Flora Tierney, Jooli Han, Bailey Ingalls, David M. Francis, Gary Li, Ulrich von Andrian, Robert Langer, Ana Jaklenec
Summary: Mucosal vaccines play a critical role in combating mucosal pathogens by directly neutralizing the invading pathogens. This review examines the role of material design in addressing the challenges associated with mucosal vaccine delivery and discusses different classes of materials currently being used.
NATURE REVIEWS MATERIALS
(2023)
Review
Immunology
Deepshikha Verma, Edward D. Chan, Diane J. Ordway
Summary: Immunity against different Mycobacteria species targeting the lung requires distinct pulmonary immune responses for bacterial clearance. Parameters of acquired and regulatory immune responses differ quantitatively and qualitatively from immunity during infection with Mycobacteria species. This discussion focuses on the potential implications of acquired and regulatory immune responses in both animal and human studies, as well as future directions for treating Mycobacteria diseases.
IMMUNOLOGICAL REVIEWS
(2021)
Article
Chemistry, Applied
Qingze Fan, Chunyu Miao, Yilan Huang, Hua Yue, Anguo Wu, Jianming Wu, Jie Wu, Guanghui Ma
Summary: The study developed HTCC-based hydrogel as a mucosal adjuvant for H5N1 vaccine, and found that with increasing DQ, the positive charge and gelation time of HTCC hydrogel increased while viscosity decreased. Results showed that HTCC hydrogel with a DQ of 41% prolonged antigen residence time in nasal cavity, resulting in the most potent systemic immune responses.
CARBOHYDRATE POLYMERS
(2021)
Review
Immunology
Victor Araujo Correa, Amanda Izeli Portilho, Elizabeth De Gaspari
Summary: Vaccines are crucial in controlling infectious diseases, but most vaccines are not effective in providing mucosal protection. More research is needed to develop vaccines that can elicit mucosal immunity and provide protection at the first site of contact with pathogens.
Article
Microbiology
Jingen Zhu, Swati Jain, Jian Sha, Himanshu Batra, Neeti Ananthaswamy, Paul B. Kilgore, Emily K. Hendrix, Yashoda M. Hosakote, Xiaorong Wu, Juan P. Olano, Adeyemi Kayode, Cristi L. Galindo, Simran Banga, Aleksandra Drelich, Vivian Tat, Chien-Te K. Tseng, Ashok K. Chopra, Venigalla B. Rao
Summary: This study reports the development of a needle-free, bacteriophage T4-based mucosal vaccine that induces strong mucosal immunity, robust humoral and cellular immune responses, and provides complete protection against various SARS-CoV-2 variants.
Article
Oncology
Rainjade Chung, James McKiernan, Nicholas Arpaia, Aurelien Marabelle, Mathieu Rouanne
Summary: BCG is a live attenuated strain of Mycobacterium bovis, initially developed as a vaccine for tuberculosis. It is the only bacterial therapy approved by the US FDA for clinical use in cancer treatment. BCG is used in patients with high-risk non-muscle invasive bladder cancer, delivered directly into the bladder after tumor resection. Modulating mucosal immunity by exposing the urothelium to BCG has been the main therapeutic approach for this type of cancer.
EUROPEAN JOURNAL OF CANCER
(2023)
Article
Immunology
Junfei Wei, Venkatesh L. Hegde, Ananta V. Yanamandra, Madison P. O'Hara, Brian Keegan, Kathryn M. Jones, Ulrich Strych, Maria Elena Bottazzi, Bin Zhan, K. Jagannadha Sastry, Peter J. Hotez
Summary: Trichuriasis is a neglected tropical disease affecting the poorest populations. Researchers are developing a vaccine composed of Tm-WAP49 protein to be delivered through intranasal immunization. Preliminary results in a mouse model showed high levels of antibody response and significant reduction in worm burden. The addition of adjuvants further improved the vaccine efficacy, inducing robust cellular responses and long-term T cell memory.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Mangalakumari Jeyanathan, Sam Afkhami, Alisha Kang, Zhou Xing
Summary: The optimal vaccination strategies for pandemic respiratory viruses must consider vaccine platform, delivery route, and regimens. Developing vaccines for respiratory infections has been challenging, as systemically administered vaccines fail to induce respiratory mucosal immunity. However, studies have shown that respiratory mucosal vaccination is superior to parenteral vaccination in inducing respiratory mucosal immunity. Recent advancements in developing respiratory mucosal vaccines for pathogens such as M. tuberculosis and SARS-CoV-2, including inhaled aerosol delivery of adenoviral-vectored vaccines, have shown significant promise.
CURRENT OPINION IN IMMUNOLOGY
(2023)
Article
Immunology
Ekramy E. Sayedahmed, Nelly O. Elshafie, Guangjun Zhang, Sulma I. Mohammed, Suryaprakash Sambhara, Suresh K. Mittal
Summary: The bovine adenovirus vector platform induces enhanced innate and adaptive immunity compared to the human adenovirus vector platform in mice.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemical Research Methods
Sakda Khoomrung, Intawat Nookaew, Partho Sen, Thorunn A. Olafsdottir, Josefine Persson, Thomas Moritz, Peter Andersen, Ali M. Harandi, Jens Nielsen
JOURNAL OF PROTEOME RESEARCH
(2020)
Article
Immunology
Craig P. McEntee, Hannah B. T. Moran, Natalia Munoz-Wolf, Alex M. Liddicoat, Elizabeth C. Carroll, Jeanette Erbo-Wern, Ivan S. Coulter, Peter Andersen, Frank Follmann, Ed C. Lavelle
Article
Immunology
Kathrin Kennerknecht, Reiner Noschka, Florian Loeffler, Stephanie Wehrstedt, Gabriel Kristian Pedersen, Daniel Mayer, Mark Grieshober, Dennis Christensen, Steffen Stenger
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2020)
Article
Instruments & Instrumentation
Carla B. Roces, Dennis Christensen, Yvonne Perrie
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2020)
Article
Immunology
Nina Dieu Nhien Tran Nguyen, Anja W. Olsen, Emma Lorenzen, Peter Andersen, Malene Hvid, Frank Follmann, Jes Dietrich
Article
Immunology
Carla B. Roces, Maryam T. Hussain, Signe T. Schmidt, Dennis Christensen, Yvonne Perrie
Article
Immunology
Kasper Romer Villumsen, Per Walter Kania, Dennis Christensen, Erling Olaf Koppang, Anders Miki Bojesen
Article
Immunology
Aneesh Thakur, Fernanda Endringer Pinto, Harald Severin Hansen, Peter Andersen, Dennis Christensen, Christian Janfelt, Camilla Foged
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Cell Biology
Carla Palma, Claudia La Rocca, Vincenzo Gigantino, Gabriella Aquino, Giovanni Piccaro, Dario Di Silvestre, Francesca Brambilla, Rossana Rossi, Fabrizia Bonacina, Maria Teresa Lepore, Matteo Audano, Nico Mitro, Gerardo Botti, Sara Bruzzaniti, Clorinda Fusco, Claudio Procaccini, Veronica De Rosa, Mario Galgani, Carlo Alviggi, Annibale Puca, Fabio Grassi, Tanja Rezzonico-Jost, Giuseppe Danilo Norata, Pierluigi Mauri, Mihai G. Netea, Paola de Candia, Giuseppe Matarese
Summary: The metabolic state is closely related to susceptibility to Mycobacterium tuberculosis infection, and controlled caloric restriction can reduce susceptibility to pulmonary MTB infection by inducing a metabolic shift and autophagy. This suggests that caloric restriction may be a feasible immunometabolic manipulation to control MTB infection and boost immunity against MTB.
Article
Medicine, General & Internal
Katharina Worzner, Daniel J. Sheward, Signe Tandrup Schmidt, Leo Hanke, Julie Zimmermann, Gerald McInerney, Gunilla B. Karlsson Hedestam, Ben Murrell, Dennis Christensen, Gabriel Kristian Pedersen
Summary: The study evaluated three different adjuvant systems combined with the SARS-CoV2 spike protein and found that they significantly enhanced antibody responses and neutralizing antibody titers. The oil-in-water emulsion system provided the most rapid response, with all adjuvanted groups developing neutralizing antibodies after a single dose. The specific CD4 T cell responses also varied depending on the adjuvant used.
Article
Pharmacology & Pharmacy
Signe Tandrup Schmidt, Dennis Christensen, Yvonne Perrie
Article
Engineering, Biomedical
Lorena Garcia-del Rio, Patricia Diaz-Rodriguez, Gabriel Kristian Pedersen, Dennis Christensen, Mariana Landin
Summary: This study proposes a novel strategy to enhance local immunity against Chlamydia trachomatis through subcutaneous and sublingual vaccination. A thermosensitive hydrogel with desirable properties is developed using artificial intelligence techniques to facilitate vaccine absorption. In vivo studies show that the combined vaccination approach enhances cellular immune responses and elicits local immune response in the genital tract.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biochemical Research Methods
Stefan Jungbluth, Hilde Depraetere, Monika Slezak, Dennis Christensen, Norbert Stockhofe, Laurent Beloeil
Summary: The COVID-19 pandemic has highlighted the importance of strategies supporting vaccine development. TRANSVAC, the European vaccine-research-infrastructure initiative, conducted a comprehensive consultation of stakeholders to determine the best way to position and sustain a European vaccine R&D infrastructure. The recommendations included expanding and improving services, enhancing access to expertise and technologies, and centralizing access to research infrastructures through an online portal.
Article
Veterinary Sciences
Marta Sistere-Oro, Gabriel K. Pedersen, Lorena Cordoba, Sergi Lopez-Serrano, Dennis Christensen, Ayub Darji
VETERINARY RESEARCH
(2020)
Article
Public, Environmental & Occupational Health
Lanfranco Fattorini, Roberta Creti, Carla Palma, Annalisa Pantosti
ANNALI DELL ISTITUTO SUPERIORE DI SANITA
(2020)