Article
Biochemistry & Molecular Biology
Diana S. M. Ottoz, Lauren C. Tang, Annie E. Dyatel, Marko Jovanovic, Luke E. Berchowitz
Summary: This research focuses on the RNA-binding protein Rim4 in yeast meiosis. The study shows that Rim4 forms translation-repressing assemblies and the assembled state allows more efficient RNA binding. The location of Rim4-binding site within the transcript determines the ability of the assemblies to repress translation. Rim4 assembly depends on its intrinsically disordered region and is regulated by the Ras/protein kinase A signaling pathway. These findings reveal a mechanism by which cells couple functional protein assemblies with stimuli to regulate cellular decisions.
Article
Oncology
Yuanyuan Ban, Yixin Tan, Xiaoling Li, Xiayu Li, Zhaoyang Zeng, Wei Xiong, Guiyuan Li, Bo Xiang, Mei Yi
Summary: This study demonstrates that overexpression of YBX1 protein in nasopharyngeal carcinoma promotes cell proliferation and invasiveness by enhancing protein synthesis of AURKA.
Review
Physiology
Alexandra Balvey, Mercedes Fernandez
Summary: Chronic liver disease is a major threat to public health globally, with obesity and aging population being the main drivers of its increasing incidence. Liver transplantation remains the only curative treatment, but is hindered by high costs and organ scarcity. Therefore, new strategies are urgently needed to prevent and reverse chronic liver disease.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Katherine E. Berman, Russell Steans, Laura M. Hertz, Julius B. Lucks
Summary: This study investigates the involvement of intermediate structures in translation controlling riboswitches, specifically focusing on the thiB riboswitch. The results demonstrate the presence of an intermediate structure called the antisequestering stem, which competes with the AD and EP folds to implement the riboswitch mechanism.
Article
Biochemistry & Molecular Biology
Xin Li, Mengyi Zhu, Min Zang, Dandan Cao, Zhengyao Xie, Haibo Liang, Zexin Bian, Tingting Zhao, Zhibin Hu, Eugene Yujun Xu
Summary: The study highlights the essential role of PUM1 in regulating cell cycle progression in ovarian somatic cells, which is crucial for follicular development and female fertility. PUM1 targets key cell cycle regulators and its loss leads to reduced granulosa cell proliferation, affecting follicular growth and fertility. This translational control mechanism mediated by PUM1 is implicated in mammalian female reproduction and may have implications in human cancer biology.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biology
Hideyuki Nakanishi
Summary: Synthetic mRNAs produced by in vitro transcription can express any transgenes without the risk of insertional mutagenesis. Preclinical studies have developed translational regulation systems to ensure high efficacy and low adverse effects by producing the appropriate amount of therapeutic proteins.
Article
Oncology
Morgane Le Bras, Noah Gorelick, Sylvain Pautet, Betty Tyler, Stephane Manenti, Nicolas Skuli, Stefania Millevoi, Anne Cammas
Summary: This study investigates the molecular mechanisms underlying protein synthesis deregulation in glioblastoma (GBM) and its impact on tumor progression and resistance to therapy. The results reveal the crucial role of RNA-binding proteins hnRNP H/F in controlling protein synthesis in GBM through overlapping mechanisms. Furthermore, hnRNP H/F are shown to potentiate cellular processes that contribute to the aggressive and resistant phenotype of GBMs, indicating their potential as therapeutic targets.
Article
Biochemistry & Molecular Biology
Jessica Nowacki, Mateo Malenica, Stefan Schmeing, Damian Schiller, Benjamin Buchmuller, Gulshan Amrahova, Peter 't Hart
Summary: RNA-binding proteins play essential roles in regulating RNA processing and function. Translational repression assays provide a convenient and cost-effective method to study their interactions with specific RNA sequences. We optimized the assay by using linear RNA sequences instead of the previously reported hairpin type sequences, allowing for the analysis of a wider range of RNA-binding proteins. Multiplication of a consensus sequence greatly enhances the signal, enabling analysis through fluorescence intensity measurements and flow cytometry.
Review
Biochemistry & Molecular Biology
Eric P. Kusnadi, Clelia Timpone, Ivan Topisirovic, Ola Larsson, Luc Furic
Summary: Translation of mRNA is a critical step in protein-coding gene expression, and translational buffering plays an important role in regulating gene expression post-transcriptionally. There are three types of translational buffering: compensation, equilibration, and offsetting, each serving to maintain protein levels despite changes in mRNA levels. This mechanism, particularly translational offsetting, is less well understood but may have significant implications in maintaining protein homeostasis and in disease states.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Janina Ehses, Melina Schlegel, Luise Schroeger, Rico Schieweck, Sophia Derdak, Martin Bilban, Karl Bauer, Max Harner, Michael A. Kiebler
Summary: This study characterizes the role of the RNA-binding protein Staufen2 in RNP assembly, revealing that Staufen2 depletion affects the expression of Ago proteins, leading to decreased global translation and increased dendritic branching. These findings suggest a mutual dependence between Staufen2 and Ago proteins in contributing to neuronal homeostasis.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Wujing Dai, Han Qu, Jack Zhang, Angkana Thongkum, Thai Nho Dinh, Kyle Kappeler, Qin M. Chen
Summary: Oxidative stress triggers de novo translation of Nrf2 protein in cells, with FUBP1 playing a regulatory role. FUBP1 promotes the attachment of 40S ribosomal subunit to Nrf2 mRNA and formation of 43S pre-initiation complex for translation initiation of Nrf2 protein under oxidative stress.
Article
Biochemistry & Molecular Biology
Stephan Pienkoss, Soheila Javadi, Paweena Chaoprasid, Marcel Holler, Johanna Rossmanith, Petra Dersch, Franz Narberhaus
Summary: The type III secretion system (T3SS) is crucial for successful host cell infection by Gram-negative pathogens, and it is coordinated by RNA-based temperature sensors at multiple levels. Components such as YscJ and YscT play important roles in T3SS assembly, and their translation is temperature-dependent.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Biology
Mickaele Hemono, Alexandre Haller, Johana Chicher, Anne-Marie Duchene, Richard Patryk Ngondo
Summary: Through the analysis of CLUH interactome, our study sheds a new light on CLUH molecular function by revealing new partners and by highlighting its link to the translation and subcellular localization of some mRNAs coding for mitochondrial proteins.
Article
Oncology
Jiahao Shi, Xiaoliang Jin, Yihao Wang, Tianyu Zhu, Dongmei Zhang, Qian Li, Xiaomin Zhong, Yaqi Deng, Jianfeng Shen, Xianqun Fan
Summary: p53 is the most highly mutated tumor suppressor in various human cancers, and its function is modulated through protein-protein interaction with MDM2. Restoring p53 function by inhibiting p53-MDM2 interaction is a promising therapeutic strategy for cancers with wild-type or functional p53. However, the biomarkers that can predict the response to these inhibitors remain elusive. In this study, LIN28B was found to directly regulate p53 by binding to its mRNA and inhibiting translation through competition with RPL26. Inhibition of LIN28B releases p53 translation suppression and enhances cancer cell sensitivities to p53-MDM2 inhibitors. These findings have important implications in developing biomarkers for therapies targeting p53 function.
Article
Chemistry, Multidisciplinary
Anna Grochmal, Ben Woods, Lilia Milanesi, Manuel Perez-Soto, Salvador Tomas
Summary: Research on mimicking biological systems reveals that minimal environmental changes can trigger high avidity membrane receptor assembly, which can be modulated by weakly binding divalent ligands. Mathematical models and equations can predict the modulation intensity of the ligand-messenger on the ligand-receptor ON signal, providing valuable tools for studying membrane receptors in biological and biomimetic systems.
Article
Biochemistry & Molecular Biology
Sandra M. Fernandez Moya, Michael A. Kiebler
Review
Neurosciences
Helena F. Pernice, Rico Schieweck, Michael A. Kiebler, Bastian Popper
Editorial Material
Biochemistry & Molecular Biology
Janina Ehses, Michael A. Kiebler, Sandra M. Fernandez-Moya
Review
Genetics & Heredity
Rico Schieweck, Bastian Popper, Michael A. Kiebler
TRENDS IN GENETICS
(2016)
Review
Biochemistry & Molecular Biology
Sandra M. Fernandez-Moya, Janina Ehses, Michael A. Kiebler
Article
Biochemical Research Methods
Karl E. Bauer, Michael A. Kiebler, Inmaculada Segura
Article
Behavioral Sciences
Bastian Popper, Antonia Demleitner, Valerie J. Bolivar, Gretchen Kusek, Abigail Snyder-Keller, Rico Schieweck, Sally Temple, Michael A. Kiebler
NEUROBIOLOGY OF LEARNING AND MEMORY
(2018)
Article
Biochemistry & Molecular Biology
Helena F. Pernice, Rico Schieweck, Mehrnoosh Jafari, Tobias Straub, Martin Bilban, Michael A. Kiebler, Bastian Popper
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Tjasa Lepko, Melanie Pusch, Tamara Mueller, Dorothea Schulte, Janina Ehses, Michael Kiebler, Julia Hasler, Hagen B. Huttner, Roosmarijn E. Vandenbroucke, Charysse Vandendriessche, Miha Modic, Ana Martin-Villalba, Sheng Zhao, Enric LLorens-Bobadilla, Anja Schneider, Andre Fischer, Christopher T. Breunig, Stefan H. Stricker, Magdalena Goetz, Jovica Ninkovic
Article
Multidisciplinary Sciences
Karl E. Bauer, Inmaculada Segura, Imre Gaspar, Volker Scheuss, Christin IIlig, Georg Ammer, Saskia Hutten, Eugenia Basyuk, Sandra M. Fernandez-Moya, Janina Ehses, Edouard Bertrand, Michael A. Kiebler
NATURE COMMUNICATIONS
(2019)
Article
Cell Biology
Bouchra Khalaf, Alessandro Roncador, Francesca Pischedda, Antonio Casini, Sabine Thomas, Giovanni Piccoli, Michael Kiebler, Paolo Macchi
JOURNAL OF CELL SCIENCE
(2019)
Article
Biotechnology & Applied Microbiology
Stefan M. Berger, Ivan Fernandez-Lamo, Kai Schoenig, Sandra M. Fernandez Moya, Janina Ehses, Rico Schieweck, Stefano Clementi, Thomas Enkel, Sascha Grothe, Oliver von Bohlen Und Halbach, Inmaculada Segura, Jose Maria Delgado-Garcia, Agnes Gruart, Michael A. Kiebler, Dusan Bartsch
Article
Cell Biology
Philipp Follwaczny, Rico Schieweck, Therese Riedemann, Antonia Demleitner, Tobias Straub, Anna H. Klemm, Martin Bilban, Bernd Sutor, Bastian Popper, Michael A. Kiebler
DISEASE MODELS & MECHANISMS
(2017)
Article
Biochemistry & Molecular Biology
Tejaswini Sharangdhar, Yoichiro Sugimoto, Jacqueline Heraud-Farlow, Sandra M. Fernandez-Moya, Janina Ehses, Igor Ruiz de los Mozos, Jernej Ule, Michael A. Kiebler