Article
Biochemistry & Molecular Biology
Mohammed S. Mansour, Karina Malmros, Ulrich Mager, Kajsa Ericson Lindquist, Kim Hejny, Benjamin Holmgren, Tomas Seidal, Annika Dejmek, Katalin Dobra, Maria Planck, Hans Brunnstrom
Summary: This study aimed to explore the potential impacts of various clinicopathological and molecular factors on PD-L1 expression. The results showed that tumor histology and mutational status were correlated with PD-L1 expression. These findings are important for further understanding the treatment response in this disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Respiratory System
Florian J. Fintelmann, Nikki A. Martin, Ismail Tahir, Elissa M. Quinn, Timothy C. Allen, Lija Joseph, Boris Nikolic, Christopher Lee
Summary: This study aimed to identify challenges and potential solutions for successful molecular testing of lung needle biopsy specimens in patients with advanced NSCLC. The primary barriers identified included biopsy request, biopsy procedure, specimen analysis, and communication. Potential solutions included adding a checkbox for molecular testing in the biopsy request, using pre-procedural imaging to guide biopsies, conserving tissue through appropriate allocation strategies and next generation sequencing panels, instituting reflex-molecular testing upon NSCLC diagnosis, tracking and communicating biopsy outcomes at tumor boards, and improving integration of radiologists and pathologists into oncology care teams.
RESPIRATORY RESEARCH
(2023)
Review
Oncology
Felice Crocetto, Biagio Barone, Matteo Ferro, Gian Maria Busetto, Evelina La Civita, Carlo Buonerba, Giuseppe Di Lorenzo, Daniela Terracciano, Jack A. Schalken
Summary: Bladder cancer is the most common malignancy of the urinary tract. Traditional diagnostic methods are invasive and uncomfortable, while alternative methods have poor sensitivity. Therefore, an improved non-invasive approach is needed for the management of bladder cancer.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2022)
Review
Clinical Neurology
Wei-Liang Chen, Heather C. Mefford
Summary: The role of genetics in epilepsy has been well recognized and genome-wide technologies have identified numerous genes and variants associated with epilepsy. There are various genetic testing options available in clinical setting and genetic testing is a dynamic process.
Review
Biochemistry & Molecular Biology
Alessandro Caputo, Angela D'Ardia, Francesco Sabbatino, Caterina Picariello, Chiara Ciaparrone, Pio Zeppa, Antonio D'Antonio
Summary: The standard of care for advanced non-small-cell lung cancer involves detecting actionable mutations, with the Idylla EGFR test being able to detect 51 mutations in 2.5 hours. Cytological material can be used for this test, but caution should be taken as the accuracy may be reduced when using scraped stained smears as input.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Go Eun Bae, Seok-Hwan Kim, Min Kyung Choi, Jin-Man Kim, Min-Kyung Yeo
Summary: Cytology from ascites and peritoneal washing fluids of gastrointestinal cancers can aid in tumor staging and prognosis. The sensitivity of detecting somatic mutations in fluid samples can be increased by assessing samples containing >2% tumor volume. Targeted NGS of ascites and peritoneal washing fluid shows clinical implications and limitations in patients with GI cancers.
FRONTIERS IN ONCOLOGY
(2021)
Article
Veterinary Sciences
Yu Furusawa, Mariko Shima-Sawa, Tatsuro Hifumi, Noriaki Miyoshi, Osamu Yamato, Akira Yabuki
Summary: In this study, the usefulness of cryopreserved air-dried smears for immunocytochemical staining was evaluated. The results showed that immunoreactivity for cytokeratin and vimentin could be detected for at least 33 months in the cryopreserved smears, demonstrating the usefulness and effectiveness of this method in veterinary immunocytochemistry.
VETERINARY SCIENCES
(2023)
Review
Oncology
Farzaneh Karimi, Omid Azadbakht, Ali Veisi, Mohammad Sabaghan, Maryam Owjfard, Ebrahim Kharazinejad, Negar Dinarvand
Summary: Ovarian cancer is a highly fatal gynecologic malignancy often diagnosed at an advanced stage which presents challenges for disease management. Conventional tissue biopsy and serological biomarkers have limitations in diagnosing and predicting the prognosis of ovarian cancer patients. Liquid biopsy, a novel sampling method involving analysis of tumor elements in peripheral blood, shows promise in improving early prognosis and diagnosis of ovarian cancer. This study reviews the utility of liquid biopsy components in ovarian cancer prognosis and diagnosis, and evaluates their advantages, limitations, and obstacles for future studies.
Review
Biochemistry & Molecular Biology
Paulina Sledzinska, Marek G. Bebyn, Jacek Furtak, Janusz Kowalewski, Marzena A. Lewandowska
Summary: The latest research on prognostic and predictive biomarkers in gliomas has been compiled, highlighting the importance of prognostic markers in adult and pediatric patients and the clinical significance in predicting treatment responses. Further work is needed to implement novel technologies such as liquid biopsies in clinical practice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Donatella Verbanac, Andrea Ceri, Iva Hlapcic, Mehdi Shakibaei, Aranka Brockmueller, Bozo Kruslin, Neven Ljubicic, Neven Barsic, Dijana Detel, Lara Baticic, Lada Rumora, Anita Somborac-Bacura, Mario Stefanovic, Ivana Celap, Alma Demirovic, Roberta Petlevski, Jozsef Petrik, Marija Grdic Rajkovic, Andrea Hulina-Tomaskovic, Ivana Rako, Luciano Saso, Karmela Barisic
Summary: Drug-specific therapeutic approaches for colorectal cancer have significantly improved patient health, but there is a need for personalized treatments based on genetic and epigenetic tumor profiles. Circulating tumor DNA analysis has proven inaccurate, but detecting circulating tumor cells and protein analysis of exosomes show promise. Blood liquid biopsy has potential for the future, if results align with tissue analyses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Christian Minnert, Karsten Durst
Summary: Different loading protocols have been developed to investigate the creep properties of materials using instrumented indentation testing technique, with a recent focus on a new indentation creep method maintaining constant contact pressure. This study compares results from constant contact pressure creep tests with uniaxial and constant load hold indentation creep experiments, revealing similar stress exponents between constant contact pressure and uniaxial tests. Additionally, a significant change in the power law exponent at large stress reductions was observed in both uniaxial and constant contact pressure tests, indicating a shift in deformation mechanism of ultrafine grained metals.
JOURNAL OF MATERIALS RESEARCH
(2022)
Article
Health Care Sciences & Services
Michael J. Duffy, John Crown
Summary: Biomarkers that can predict patients' response to specific therapies are crucial for personalized cancer treatment. Tumor tissue is the gold standard for determining predictive biomarkers, but it is not always available or adequate for analysis. In contrast, circulating tumor DNA (ctDNA) in the blood can be obtained from almost all patients in a minimally invasive and safe manner. Blood-based biomarker testing has advantages such as shorter turnaround time and the ability to perform serial measurements. Although tissue remains the gold standard, blood-based predictive biomarkers can be used when tissue is unavailable.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Lukas Lueftinger, Ines Ferreira, Bernhard J. H. Frank, Stephan Beisken, Johannes Weinberger, Arndt von Haeseler, Thomas Rattei, Jochen G. Hofstaetter, Andreas E. Posch, Arne Materna
Summary: This review summarizes the current developments in next-generation sequencing based predictive antibiotic susceptibility testing and discusses the potential and limitations for common PJI pathogens.
Article
Oncology
Kei Morikawa, Hirotaka Kida, Hiroshi Handa, Takeo Inoue, Hisashi Saji, Junki Koike, Seiji Nakamura, Yoshiharu Sato, Yumi Ueda, Fumihiko Suzuki, Ryo Matoba, Masamichi Mineshita
Summary: This study prospectively evaluated the feasibility of using cytological specimens for lung cancer gene panel testing. The results showed that cytological specimens were suitable for gene analysis and had high correlations with tissue specimens for detecting gene mutations.
Article
Oncology
Janna Siemanowski, Birgid Schoemig-Markiefka, Theresa Buhl, Anja Haak, Udo Siebolts, Wolfgang Dietmaier, Norbert Arens, Nina Pauly, Beyhan Ataseven, Reinhard Buettner, Sabine Merkelbach-Bruse
Summary: This study focused on challenges in microsatellite instability testing in endometrial cancer and compared four different PCR-based approaches to improve MSI testing in future screenings. By directly comparing the systems, the study was able to highlight the advantages and limitations of each.
Editorial Material
Pathology
Fang Zhou, Alan Marcus
INTERNATIONAL JOURNAL OF SURGICAL PATHOLOGY
(2021)
Article
Oncology
Edward T. Carey, Virginia Ferreira, Elaine Shum, Fang Zhou, Joshua K. Sabari
Summary: Patients with lung cancer are at increased risk for multiple primary lung cancers, which require thorough diagnostics and individualized treatment options.
CLINICAL LUNG CANCER
(2022)
Review
Cell Biology
Fang Zhou, Elaine Shum, Andre L. Moreira
Summary: Molecular testing is feasible on various cytological preparations, but rigorous validation is necessary for different fixatives and cytology preparations before implementation. This is crucial for successful molecular testing in a clinical setting.
Article
Pathology
Fang Zhou, Julian A. Villalba, Treah May S. Sayo, Navneet Narula, Harvey Pass, Mari Mino-Kenudson, Andre L. Moreira
Summary: Intraoperative detection of STAS on frozen sections has low sensitivity and positive predictive value, while the determination of tumor grade on frozen sections offers better sensitivity and specificity, and is associated with recurrence-free survival. Further study is needed to explore the utility of assessing tumor grade on frozen sections.
Article
Pathology
Abdallah Flaifel, Benjamin Kwok, Jane Ko, Stephanie Chang, Deane Smith, Fang Zhou, Luis A. Chiriboga, Briana Zeck, Neil Theise, Darya Rudym, Melissa Lesko, Luis Angel, Andre Moreira, Navneet Narula
Summary: This study reports on the clinical course, pulmonary pathology, and outcomes after lung transplantation in three patients with chronic respiratory failure due to postacute sequelae of SARS-CoV-2 infection. The major pathological findings in the lung explants suggest ongoing fibrosis, prominent macrophage infiltration, neoangiogenesis, and microvascular thrombosis.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2022)
Review
Oncology
Jonathan Willner, Fang Zhou, Andre L. Moreira
Summary: Thymic epithelial neoplasms, including thymoma, thymic carcinoma, and thymic neuroendocrine neoplasms, are rare tumors that make up the majority of anterior mediastinal masses. Fine needle aspirations (FNA) of these masses are challenging to interpret due to the histologic diversity of thymic neoplasms and overlapping features with tumors from other sites. However, with the use of ancillary studies and radiology, FNAs can provide clinically actionable results. This review discusses the utility and limitations of thymic FNAs and highlights the diagnostic features and pitfalls of these specimens.
Article
Pathology
Issa Hindi, Aylin Simsir, Oliver Szeto, Osvaldo Hernandez, Wei Sun, Fang Zhou, Tamar C. Brandler
Summary: This study assessed the benefits of the Milan System for Reporting Salivary Gland Cytopathology (MSRSGC) over the original cytology classification and measured interobserver agreement. The results showed that MSRSGC performs similarly to the original cytology classification and has relatively high interobserver agreement.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2022)
Article
Medical Laboratory Technology
Lawrence Hsu Lin, Fang Zhou, Esther Elishaev, Samer Khader, Andrea Hernandez, Alan Marcus, Esther Adler
Summary: This study aimed to evaluate cervicovaginal cytology specimens and high-risk HPV testing in transmasculine individuals receiving testosterone. The study found that transmasculine individuals showed higher rates of abnormal Pap tests, and testosterone seemed to induce changes in squamous cells and shifts in vaginal flora. HR-HPV testing can be a useful adjunct in the workup of abnormal Pap tests from transmasculine individuals.
DIAGNOSTIC CYTOPATHOLOGY
(2022)
Review
Cardiac & Cardiovascular Systems
Jonika M. Weerasekare, Fang Zhou, Adam H. Skolnick, Hasan Jilaihawi, Mathew R. Williams, Surendra Dasari, Ellen D. McPhail, Jason D. Theis, Linda N. Dao, John P. Bois, Joseph J. Maleszewski, Melanie C. Bois
Summary: This article presents a case study of an 80-year-old man who underwent aortic valve replacement 12 years ago and subsequently required re-replacement due to calcification. The surgically explanted valve tissue showed amyloid deposits with specific protein composition. The amyloid was classified as Mu heavy chain, which is uncommon in bioprosthetic valves. The article also provides a literature review on isolated amyloid deposition in aortic valves.
CARDIOVASCULAR PATHOLOGY
(2022)
Article
Otorhinolaryngology
Abhinav Talwar, Evan Patel, Moses Tam, Fang Zhou, Kenneth Hu, Michael Persky, Alec Vaezi, Adam Jacobson, Babak Givi
Summary: Carcinosarcoma of the salivary gland is a rare malignant tumor that frequently presents at a locally advanced stage and has poor prognosis. Age and tumor stage are important factors in predicting survival.
OTOLARYNGOLOGY-HEAD AND NECK SURGERY
(2023)
Article
Multidisciplinary Sciences
Yin -Hu Wang, Lucile Noyer, Sascha Kahlfuss, Dimitrius Raphael, Anthony Y. Tao, Ulrike Kaufmann, Jingjie Zhu, Marisa Mitchell-Flack, Ikjot Sidhu, Fang Zhou, Martin Vaeth, Paul G. Thomas, Sean P. Saunders, Kenneth Stauderman, Maria A. Curotto de Lafaille, Stefan Feske
Summary: The study reveals that SOCE through CRAC channels plays a crucial role in regulating T cell function, particularly in TH2 cells, by controlling the expression of transcription factors and cytokines. Inhibition of SOCE shows potential as a treatment for allergic airway disease.
Review
Radiology, Nuclear Medicine & Medical Imaging
Francis Girvin, Fang Zhou, Joanna Escalon, Sharon Steinberger, Deepti Gupta, Lauren Groner
Summary: This article reviews and illustrates the anatomy, CT appearances, and potential etiologies of granulomatous lymphadenitis caused by the inferior pulmonary ligament and related connective tissue septa, which can mimic lung parenchymal lesions on chest CT.
Editorial Material
Cardiac & Cardiovascular Systems
Arushi Singh, Bernard S. Kadosh, Kelsey Grossman, Robert Donnino, Navneet Narula, Fang Zhou, Michael Divita, Deane E. Smith, Nader Moazami, Stephanie H. Chang, Luis F. Angel, Alex Reyentovich
CIRCULATION-HEART FAILURE
(2023)
Article
Pathology
Reid Wilkins, Elcin Zan, Olga Leonardi, Kepal N. Patel, Adam S. Jacobson, George Jour, Cheng Z. Liu, Fang Zhou
Summary: This article reports a rare case of lipomatous thyroid tumor, which was initially non-diagnostic through fine needle aspiration and eventually diagnosed as a benign pathology after surgical removal and pathological examination. Genetic testing revealed multiple genetic alterations in this case, supporting the concept of angiolipomas being true neoplasms. The case suggests that conservative surgical management can be considered for lipomatous thyroid tumors before a final pathological diagnosis is determined.
HEAD & NECK PATHOLOGY
(2023)
Article
Otorhinolaryngology
Alex J. Gordon, Aneek Patel, Fang Zhou, Cheng Liu, Amit Saxena, Paula Rackoff, Babak Givi
Summary: This study aims to assess the adequacy and risk of complications of minor salivary gland biopsy in the diagnosis of Sjogren's syndrome. The results show that this biopsy method is safe and effective, providing clinically meaningful information and can be recommended for patients suspected to have Sjogren's syndrome.