Journal
MICROMACHINES
Volume 10, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/mi10070462
Keywords
circulating tumor cell; white blood cell; sheathless; high-throughput; viscoelastic fluid; separation
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Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2017R1D1A1A09000962]
- National Research Foundation of Korea [2017R1D1A1A09000962] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Circulating tumor cells (CTCs) have attracted increasing attention as important biomarkers for clinical and biological applications. Several microfluidic approaches have been demonstrated to separate CTCs using immunoaffinity or size difference from other blood cells. This study demonstrates a sheathless, high-throughput separation of CTCs from white blood cells (WBCs) using a viscoelastic fluid. To determine the fluid viscoelasticity and the flow rate for CTC separation, and to validate the device performance, flow characteristics of 6, 13, and 27 mu m particles in viscoelastic fluids with various concentrations were estimated at different flow rates. Using 0.2% hyaluronic acid (HA) solution, MCF-7 (Michigan Cancer Foundation-7) cells mimicking CTCs in this study were successfully separated from WBCs at 500 mu L/min with a separation efficiency of 94.8%. Small amounts of MCF-7 cells (similar to 5.2%) were found at the center outlet due to the size overlap with WBCs.
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