Article
Chemistry, Multidisciplinary
Long Yu, Vincent Bulone
Summary: In this study, the extraction and de-glycosylation of quercetin derivatives from apple pomace using deep eutectic solvents (DES) was successfully achieved. It was found that DES extraction resulted in higher de-glycosylation of quercetin derivatives and increased antioxidant activity compared to conventional methanol extraction.
Article
Food Science & Technology
Luna Pollini, Ana Juan-Garcia, Francesca Blasi, Jordi Manes, Lina Cossignani, Cristina Juan
Summary: Apple pomace, a by-product of the fruit processing industry, was studied for its potential phenolic compounds using in vitro bioaccessibility tests. Results showed that chlorogenic acid had the highest bioaccessibility among the tested compounds. This study suggests that apple pomace could be a promising ingredient for developing new functional foods.
Article
Biochemistry & Molecular Biology
Luna Pollini, Lina Cossignani, Cristina Juan, Jordi Manes
Summary: Red Delicious apple pomace was produced using a domestic blender and various non-conventional extraction techniques were tested to isolate phenolic compounds. The most efficient method to recover polyphenols was identified, highlighting the potential value of apple pomace as a by-product rich in phenolic compounds.
Article
Food Science & Technology
Shaziya Manzoor, F. A. Masoodi, Rubiya Rashid, Mohd Masrat Dar
Summary: This study evaluated the stability of high oleic mustard oil during deep frying of French fries using apple pomace-based antioxidants. The results showed that mustard oil incorporated with these antioxidants exhibited higher stability and quality compared to synthetic antioxidants.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Shaziya Manzoor, F. A. Masoodi, Rubiya Rashid, Mohd Masrat Dar
Summary: The study found that apple pomace extract positively affects the oxidative stability of soybean oil during frying, outperforming other antioxidants. Oils enriched with natural antioxidants show better thermal stability and quality, potentially replacing synthetic antioxidants in deep-fried oils effectively.
Article
Biochemistry & Molecular Biology
Keren Bindon, Song Qi, Stella Kassara, Luca Nicolotti, Alicia Jouin, Maggie Beer
Summary: Recent research has focused on improving the use of apple pomace waste in the food and nutraceutical industries. This study quantified the polymeric procyanidins in apple pomace extracts for the first time and found that they were the most abundant polyphenol type. The presence of polymeric procyanidins in apple pomace extracts may overestimate their antioxidant activity in vivo.
Article
Biotechnology & Applied Microbiology
Paula Sol Pok, Victor Alonso Garcia Londono, Sebastian Vicente, Ana Pacin, Stella Maris Alzamora, Silvia Liliana Resnik
Summary: The use of flavonoids from citrus industry residues can effectively reduce the accumulation of fumonisins in maize and cause destructive effects on the cell structure of Fusarium, offering a potential natural and environmentally friendly antifungal treatment for stored maize.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jung-A Ryu, Shucheng Duan, Ho-Young Jeong, Chanhui Lee, In-Kyu Kang, Seok Hyun Eom
Summary: This study analyzed the diversity of flavonoids and the patterns of flavonoid metabolic gene expression in immature and color-turning 'Fuji' apples under light irradiation with or without methyl jasmonate treatment. The results showed that UV-B exposure significantly stimulated anthocyanin accumulation in apple skin, and the immature stage of apples was more sensitive to UV-B and MeJA treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Arti Nile, Shivraj Hariram Nile, Juhyun Shin, Gyunseok Park, Jae-Wook Oh
Summary: Quercetin-3-glucoside extracted from apple pomace exhibits potent antioxidant and anti-inflammatory effects, as well as significant cytotoxic effects on cervical cancer cells. Results show that Q3G induces cell cycle arrest and apoptosis in HeLa cells by altering key proteins, suggesting its potential as an anti-inflammatory and anticancer agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Y. N. Duan, W. T. Jiang, R. Zhang, R. Chen, X. S. Chen, C. M. Yin, Z. Q. Mao
Summary: This study revealed that F. proliferatum is the main pathogenic fungus causing ARD in China, which is of great significance for controlling ARD.
Article
Biotechnology & Applied Microbiology
Ran Shi, Shao-qing Yang, Nan-nan Wang, Qiao-juan Yan, Xie-min Yan, Zheng-qiang Jiang
Summary: In this study, a recombinant xyloglucanase and an a-L-fucosidase gene were used to convert XyG-oligos from apple pomace into 2'-FL, demonstrating an efficient enzymatic strategy for synthesizing 2'-FL.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yanan Duan, Shurui Ma, Xuesen Chen, Xiang Shen, Chengmiao Yin, Zhiquan Mao
Summary: RNA-seq profiling was conducted to analyze the response of apple roots to Fpmd MR5 infection, revealing that the activation of resistance genes in apple roots plays a crucial role in defending against the pathogen.
Article
Plant Sciences
Y. N. Duan, S. R. Ma, X. S. Chen, X. Shen, C. M. Yin, Z. Q. Mao
Summary: Apple replant disease (ARD) caused by Fusarium proliferatum f. sp. malus domestica (Fpmd) MR5 is a fungal pathogen that leads to annual losses in apple production in China. In this study, the whole-genome sequence of the highly virulent Fpmd MR5 was obtained, revealing potential pathogenicity-related genes and specific virulence factor genes. These findings will contribute to further research on host-pathogen interactions and the development of disease management strategies.
Article
Plant Sciences
Alin Din, Denisa S. Vilcoci, Georgiana Cirstea, Denis Negrea, Sorin Moga, Cristina Mihaescu, Rodi Mitrea
Summary: This study utilized waste from the vinification process to extract bioactive compounds with significant antifungal properties, particularly against Fusarium sp. and Alternaria sp. The highest amount of polyphenols was found in the microwave-assisted extract, while the macerated extract showed the highest antioxidant activity.
NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA
(2022)
Article
Agriculture, Multidisciplinary
Francisco Maldonado, Jose A. Yuri, Amalia Neira, Ivan Razmilic
Summary: This study aimed to evaluate the effects of organic and conventional management practices, cultivar, and fruit growth stage on the total phenolic content, antioxidant activity, and quercetin glycosides in apples. The results showed that cultivar and fruit growth stage were important factors affecting the chemical composition of apples, while the management practices had a significant impact on the concentration of quercetin glycosides.
SPANISH JOURNAL OF AGRICULTURAL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Wiktoria Maj, Giorgia Pertile, Magdalena Frac
Summary: Soil-borne Neosartorya spp. are highly resilient fungi that can excrete mycotoxins and have great resistance to high temperatures. They easily transfer between soil and crops, posing a threat to horticulture and food production. Despite being a cause of spoilage in fruit produce, juices, and pulps, these fungi can also participate in carbon transformation, sequestration, and plant protection in drought conditions. The taxonomical controversy surrounding Neosartorya spp. and its resemblance to its anamorph leads to uncertain data in research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Barbara Abramczyk, Lukasz Pecio, Solomiia Kozachok, Mariusz Kowalczyk, Anna Marzec-Grzadziel, Ewa Krol, Anna Galazka, Wieslaw Oleszek
Summary: Fungi from the genus Diaporthe can be plant pathogens, endophytes, and saprophytes on various host plants worldwide. Identifying Diaporthe species is challenging due to their ability to colonize multiple host plants. Moreover, Diaporthe has been found to produce bioactive secondary metabolites, particularly the drimane sesquiterpenoid-phthalide hybrid class. The study analyzed 40 Diaporthe isolates and identified 153 compounds, including 43 fungal metabolites that could serve as chemotaxonomic markers. This research provides valuable insights for future studies on the isolation and bioactive potential of these metabolites for agricultural applications.
Article
Biochemistry & Molecular Biology
Jaroslaw Widelski, Katarzyna Gawel-Beben, Karolina Czech, Emil Paluch, Olga Bortkiewicz, Solomiia Kozachok, Tomasz Mroczek, Piotr Okinczyc
Summary: Tyrosinase is a key enzyme in melanogenesis, and its hyperactivity can cause hyperpigmentation disorders. This study assessed the tyrosinase inhibitory potential of six propolis extracts and found that they showed potent inhibitory activity. The extracts also exhibited antioxidant activity and various phytochemical composition. These findings suggest that the tested propolis extracts could be used in cosmeceutical and medical applications.
Review
Biochemistry & Molecular Biology
Marta Oleszek, Iwona Kowalska, Terenzio Bertuzzi, Wieslaw Oleszek
Summary: Billions of tons of agro-industrial residues are produced worldwide, posing pollution and economic challenges. However, the non-edible parts of crops have valuable bioactive compounds. Therefore, it is crucial to develop methods for utilizing these residues to produce high-value by-products.
Article
Chemistry, Applied
Lukasz Pecio, Solomiia Kozachok, Fatema R. Saber, Maria Garcia-Marti, Yasser El-Amier, Engy A. Mahrous, Lukasz Swiatek, Anastazja Boguszewska, Adrianna Skiba, Ahmed H. Elosaily, Krystyna Skalicka-Wozniak, Jesus Simal-Gandara
Summary: In this study, the cytotoxic activity of Ochradenus baccatus Delile, a desert plant native to the Middle East, was evaluated using cell lines and a zebrafish embryo model. The extract exhibited the highest cytotoxicity against human cervical adenocarcinoma cells, with a CC50 of 39.1 μg/mL and a selectivity index of 7.23. Metabolic analysis identified various compounds in the extract, including fatty acids, flavonoids, glucosinolates, nitrile glycosides, and organic acids. These phytochemicals may contribute to the observed cytotoxic activity. O. baccatus has the potential to be a safe food source to help meet the increasing food demand in Saharan countries.
Article
Chemistry, Applied
Fatema R. Saber, Ahmed H. Elosaily, Engy A. Mahrous, Lukasz Pecio, Solomiia Pecio, Yasser A. El-Amier, Maciej Korczak, Jakub P. Piwowarski, Lukasz Swistek, Krystyna Skalicka-Wozniak
Summary: Wild edible plant Urospermum picroides is a potential source for expanding and diversifying the global food supply, with this study revealing its chemical profile and the identification of 77 metabolites, including 12 novel sesquiterpene-amino acid conjugates. Molecular networking analysis provided insight into the fragmentation pathway of these conjugates. Additionally, the sesquiterpene-enriched U. picroides extract demonstrated a moderate anti-inflammatory effect, highlighting its potential as a functional food and nutraceutical agent.
Article
Biochemistry & Molecular Biology
Lukasz Pecio, Mostafa Alilou, Solomiia Kozachok, Ilkay Erdogan Orhan, Gokcen Eren, Fatma Sezer Senol Deniz, Hermann Stuppner, Wieslaw Oleszek
Summary: The absolute configurations of spiro-flavostilbenoids found in the bark of Yucca schidigera were determined using density functional theory simulation. The compounds were found to be biosynthetic derivatives of (2R)-naringenin and trans-resveratrol or trans-3,3',5,5'-tetrahydroxy-4'-methoxystilbene. Gloriosaols were direct derivatives of yuccaols. Yuccaol B and gloriosaol A showed moderate inhibitory effects against acetyl-/butyrylcholinesterases.
Review
Biochemistry & Molecular Biology
Lukasz Pecio, Solomiia Pecio, Tomasz Mroczek, Wieslaw Oleszek
Summary: Based on literature data from 1973 to 2022, this review summarizes the research on spiro-flavonoids with a spiro-carbon at the center of their structure, including their isolation methods, stereochemistry, and biological activity. The review includes 65 unique structures of spiro-flavonoids from different families and discusses their biosynthetic pathways. The most important biological activities of spiro-flavonoids are anti-inflammatory and anticancer effects, observed both in vitro and in vivo. The review also identifies the challenges in stereochemistry assignment and provides future research perspectives.
Article
Plant Sciences
Ana Flavia Burlec, Lukasz Pecio, Cornelia Mircea, Cristina Tuchilus, Andreia Corciova, Corina Danciu, Oana Cioanca, Ioana Cezara Caba, Solomiia Pecio, Wieslaw Oleszek, Monica Hancianu
Summary: Black-eyed Susan, a flowering plant with traditional medicinal uses, has been studied for its therapeutic properties. The chemical composition and bioactivity of black-eyed Susan cultivated in Romania were comprehensively investigated. The extract showed inhibitory activity against bacteria and fungi, demonstrated antioxidant properties, and exhibited potential anticancer effects. This research provides valuable information and paves the way for future investigation of the plant's potential applications in the pharmaceutical and nutraceutical sectors.
Article
Pharmacology & Pharmacy
Pawel Gumulka, Lukasz Pecio, Pawel Zmudzki, Krzesimir Ciura, Krystyna Skalicka-Wozniak, Monika Dabrowska, Malgorzata Starek
Summary: Understanding the influence of environmental factors on drug stability is crucial for determining excipients, storage conditions, and packaging materials. In this study, a thin-layer chromatographic-densitometric procedure and UPLC-MS/MS were used to analyze five coxibs. The results showed that the drugs were more stable in alkaline environments compared to acidic environments. Robenacoxib decomposed the fastest, while cimicoxib was the most stable. Additionally, chemical analysis and hepatotoxicity tests using a human liver cancer cell line were conducted to identify potential degradation pathways and toxic effects of the degradation products.
