Article
Chemistry, Multidisciplinary
Zhiyuan Lu, Lixia Huang, Yanxia Li, Yan Xu, Ruihao Zhang, Qian Zhou, Qi Sun, Yi Lu, Junjie Chen, Yuemao Shen, Jian Li, Baobing Zhao
Summary: This study reveals the crucial role of cholesterol in terminal erythropoiesis, showing that reduced cholesterol levels inhibit erythroid proliferation and regulate the differentiation process. Fine-tuning of cholesterol homeostasis emerges as a key mechanism for regulating erythropoiesis.
Article
Immunology
Minmin Wu, Ran An, Nan Zhou, Ying Chen, Haijian Cai, Qi Yan, Ru Wang, Qingli Luo, Li Yu, Lijian Chen, Jian Du
Summary: IFN-gamma-activated macrophages can inhibit the replication of Toxoplasma gondii less virulent strain and promote the localization of immunity-related GTPases and guanylate-binding proteins through interaction with host autophagy proteins, thus controlling the proliferation of parasites.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rafael M. Oliveira, Thaise L. Teixeira, Cassiano C. Rodrigues, Aline A. da Silva, Bruna C. Borges, Rebecca T. S. Brigido, Samuel C. Teixeira, Marlus A. Dos Santos, Joao Paulo S. Servato, Debora de O. Santos, Marcelo J. B. Silva, Luiz R. Goulart, Claudio da Silva
Summary: The study revealed the critical role of gal-3 in the host response to infection by L. (L.) amazonensis, controlling parasite invasion, replication, and formation of endocytic vesicles. Mice with gal-3 deficiency showed an exacerbated inflammatory response.
Article
Immunology
Jing Hong, Chizu Sanjoba, Wataru Fujii, Junya Yamagishi, Yasuyuki Goto
Summary: Leishmaniasis is a disease caused by infection with Leishmania parasites, which can lead to multinucleation of macrophages. Infection with Leishmania donovani induces multinucleation of bone marrow-derived macrophages (BMDMs), accompanied by increased expression of ATP6V0D2 protein. These infection-induced multinucleated macrophages exhibit enhanced phagocytic activity on red blood cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Editorial Material
Parasitology
Markus Ganter, Friedrich Frischknecht
Summary: Plasmodium merozoites invade erythrocytes through ligand binding, calcium signaling, and membrane deformation in a stepwise manner. Geoghegan et al. utilized a newly developed light sheet microscope to investigate invasion with unprecedented temporal resolution, revealing the roles of host cell cholesterol and pore formation at the parasite-host cell interface.
TRENDS IN PARASITOLOGY
(2021)
Article
Immunology
Olivier Seguin, Linh Thuy Mai, Hamlet Acevedo Ospina, Marie-Michele Guay-Vincent, Sidney W. Whiteheart, Simona Stager, Albert Descoteaux
Summary: This study investigates the contribution of host cell soluble N-ethylmaleimide-sensitive-factor attachment protein receptor proteins to the expansion and functionality of communal vacuoles as well as the replication of Leishmania parasites. The results show that VAMP8 contributes to the optimal expansion of communal vacuoles, while VAMP3 negatively regulates L. amazonensis replication, vacuole size, and antigen cross-presentation.
INFECTION AND IMMUNITY
(2022)
Article
Multidisciplinary Sciences
Sameh Obeid, Eloisa Berbel-Manaia, Valerie Nicolas, Indira Dennemont, Julien Barbier, Jean-Christophe Cintrat, Daniel Gillet, Philippe M. Loiseau, Sebastien Pomel
Summary: This study aims to investigate the mechanism of action of an antileishmanial drug candidate VP343, and it was found that VP343 inhibits the survival of Leishmania infantum by interfering with host cell vesicular trafficking and cholesterol metabolism pathways.
Article
Endocrinology & Metabolism
Algerta Marku, Lorenzo Da Dalt, Alessandra Galli, Nevia Dule, Paola Corsetto, Angela Maria Rizzo, Annalisa Moregola, Patrizia Uboldi, Fabrizia Bonacina, Paola Marciani, Michela Castagna, Alberico Luigi Catapano, Giuseppe Danilo Norata, Carla Perego
Summary: This study reveals that pancreatic PCSK9 is a critical regulator of beta-cell function, affecting insulin secretion and cellular function through modulation of cholesterol homeostasis.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2022)
Article
Medicine, Research & Experimental
Huan Tao, Patricia G. Yancey, John L. Blakemore, Youmin Zhang, Lei Ding, W. Gray Jerome, Jonathan D. Brown, Kasey C. Vickers, MacRae F. Linton
Summary: SR-BI protein plays a crucial role in atherosclerosis by regulating autophagy, with its deletion leading to increased foam cell formation and apoptosis. Additionally, SR-BI affects autophagy gene expression by modulating TFEB levels and recruiting the VPS34-Beclin-1 complex.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Biochemistry & Molecular Biology
Kai Chen, Ozlem Gunay-Esiyok, Melissa Klingeberg, Stephan Marquardt, Thomas Guenther Pomorski, Nishith Gupta
Summary: By studying Toxoplasma gondii, it was found that the parasite can salvage phosphatidylserine and phosphatidylethanolamine from its environment but not phosphatidylcholine, implying the significance of exogenous lipids in membrane biogenesis of the parasite.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Mathematical & Computational Biology
Hang Hu, Zhenxiao Lu, Haisong Feng, Guojun Chen, Yongmei Wang, Congshan Yang, Zhenyu Yue
Summary: In this paper, the researchers developed the Dense Granule Protein Database (DGPD), a knowledge database dedicated to the integration and analysis of typical GRAs properties. The database includes annotated GRAs metadata from multiple sources and was used to explore the characteristics and prediction algorithms of GRAs. The researchers hope that DGPD will be a useful resource for studying GRAs.
DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION
(2022)
Article
Biochemistry & Molecular Biology
Most Naoshia Tasnin, Tsuneyuki Takuma, Tasnuva Sharmin, Shamsul Morshed, Takashi Ushimaru
Summary: The study demonstrates that in budding yeast, nutrient starvation and TORC1 inactivation trigger micronucleophagy at the nucleus-vacuole junction (NVJ), leading to condensation of rDNA and movement of nucleolar proteins towards the NVJ for degradation. These findings suggest that the NVJ plays a critical role in the directional movements of rDNA and nucleolar proteins.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Plant Sciences
Hao-ran Liu, Chao Shen, Danial Hassani, Wan-qi Fang, Zhi-yi Wang, Yi Lu, Rui-liang Zhu, Qiong Zhao
Summary: This article summarizes the known knowledge about the morphological, metabolic Constitution properties, and biogenesis mechanism of vacuoles in bryophytes. By comparing the differences in molecular mechanisms among different species and comparing bryophytes with seed plants, a possible mechanism for the biogenesis of vacuoles in bryophytes is proposed.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Parasitology
Congshan Yang, Chenrong Wang, Jing Liu, Qun Liu
Summary: This study utilized BioID technology to identify novel components of Neospora caninum dense granules, shedding light on the biological functions of GRA proteins. 11 new GRA proteins were confirmed to be localized in the Nc1 strain.
PARASITES & VECTORS
(2021)
Article
Plant Sciences
Ting Ma, Jun-Ru Tan, Yan Zhang, Sha Li
Summary: Arabidopsis atypical R-SNARE protein YKT61 plays a key role in brassinosteroid (BR)-mediated root development by modulating the dynamic targeting of the BR receptor BRASSINOSTEROID-INSENSITIVE1.
Article
Immunology
Eduardo Milton Ramos-Sanchez, Luiza Campos Reis, Marina de Assis Souza, Sandra Marcia Muxel, Kamila Reis Santos, Dimitris Lagos, Valeria Rego Alves Pereira, Maria Edileuza Felinto de Brito, Paul Martin Kaye, Lucile Maria Floeter-Winter, Hiro Goto
Summary: This study investigated the changes in miRNA associated with leishmaniasis and identified different target pathways in immune and inflammatory pathways. miR-548d-3p may play a role in suppressing parasite growth, but further research is needed to understand its in vivo effects and its potential as a biomarker for progressive leishmaniasis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Thaise Lara Teixeira, Miguel Angel Chiurillo, Noelia Lander, Cassiano Costa Rodrigues, Thiago Souza Onofre, Eden Ramalho Ferreira, Camila Miyagui Yonamine, Julia de Gouveia Santos, Renato Arruda Mortara, Claudio Vieira da Silva, Jose Franco da Silveira
Summary: P21 is an immunomodulatory protein in Trypanosoma cruzi that plays crucial roles in host cell invasion, internalization, and amastigote proliferation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Bruno Souza Bonifacio, Alexis Bonfim-Melo, Renato Arruda Mortara, Eden Ramalho Ferreira
Summary: This study investigates the invasion mechanism of Trypanosoma cruzi and reveals the crucial role of the host cell actin cytoskeleton in the process.
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2022)
Correction
Immunology
Eduardo Milton Ramos-Sanchez, Luiza Campos Reis, Marina de Assis Souza, Sandra Marcia Muxel, Kamila Reis Santos, Dimitris Lagos, Valeria Rego Alves Pereira, Maria Edileuza Felinto de Brito, Paul Martin Kaye, Lucile Maria Floeter-Winter, Hiro Goto
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Immunology
Artur Leonel de Castro Neto, Jose Franco da Silveira, Renato Arruda Mortara
Summary: This article discusses the roles of virulence molecules in insect vectors, showing the similarities and differences in their mode of action compared to humans. It also explores the genetic events that may have led to protein diversity in these vectors.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Carolina Menezes Suassuna de Souza, Ewerton de Souza Lima, Raphael Ferreira Ordonho, Bianca Rafaella Rodrigues dos Santos Oliveira, Rebeca Cordeiro Rodrigues, Marquiliano Farias de Moura, Daniel Magalhaes Lima, Maiara Garcia Blagitz, Eduardo Milton Ramos Sanchez, Isac Almeida de Medeiros, Fernando Nogueira Souza, Artur Cezar de Carvalho Fernandes
Summary: This study evaluated the expression of immune checkpoints PD-1 and CTLA-4 in blood T lymphocytes during the periparturient period in dairy cows. The expression of these immune checkpoints was found to be associated with uterine health and immune tolerance. Additionally, the serum acute phase-protein haptoglobin concentration was related to the expression of these immune checkpoints. However, there was no association between the indicators of negative energy balance and the expression of the immune checkpoint markers.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biology
Julia Ribeiro da Silva Vallim, Gabriela Sant'Ana Lima, Heloisa Mayumi Suyama Tsuji, Beatriz Duarte Palma Xylaras, Sergio Tufik, Marcelo Demarzo, Vania D'Almeida
Summary: This study aimed to identify the sociodemographic factors associated with subjective well-being and describe the mediating behavioral variables. The findings showed that morningness, male gender, not working while studying, and practicing Pilates/yoga were associated with greater subjective well-being. Except for employment status, no direct effects were observed, highlighting the need for a multidimensional approach. The relationship between subjective well-being and sociodemographic factors exists only in the presence of behavioral mediators, specifically perceived stress, daytime sleepiness, symptoms of depression, sleep quality, and positive and negative affects. Future research should further investigate the impact of sleep, stress, and circadian preferences on this relationship.
CHRONOBIOLOGY INTERNATIONAL
(2023)
Article
Agriculture, Dairy & Animal Science
Fernando N. Souza, Kamila R. Santos, Jose A. Ferronatto, Eduardo M. Ramos Sanchez, Bruno Toledo-Silva, Marcos B. Heinemann, Sarne De Vliegher, Alice M. M. P. Della Libera
Summary: This study investigated whether different staphylococcal and mammaliicoccal species and strains can induce B and T lymphocyte proliferation and production of interleukin-17A (IL-17A) and interferon-gamma (IFN-gamma) in peripheral blood mononuclear cells (PBMCs) of nulliparous, primiparous, and multiparous dairy cows. Flow cytometry was used to measure lymphocyte proliferation, specific antibodies were used to identify different lymphocyte populations, and the PBMC culture supernatant was used to measure IL-17A and IFN-gamma production. The results showed that certain strains of Staphylococcus aureus and Staphylococcus chromogenes triggered T lymphocyte proliferation and increased IL-17A and IFN-gamma production. Conversely, Mammaliicoccus fleurettii and other strains had no effect on lymphocyte proliferation. Multiparous cows had higher B lymphocyte proliferation and cytokine production compared to primiparous and nulliparous cows. Phytohemagglutinin M-form specifically stimulated T cell proliferation.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Multidisciplinary Sciences
Mahyumi Fujimori, Ruth Tamara Valencia-Portillo, Jose Angelo Lauletta Lindoso, Beatriz Julieta Celeste, Roque Pacheco de Almeida, Carlos Henrique Nery Costa, Alda Maria da Cruz, Angelita Fernandes Druzian, Malcolm Scott Duthie, Carlos Magno Castelo Branco Fortaleza, Ana Lucia Lyrio de Oliveira, Anamaria Mello C. Miranda Paniago, Igor Thiago Queiroz, Steve Reed, Aarthy Vallur, Hiro Goto, Maria Carmen Arroyo Sanchez
Summary: The performance of recombinant antigens K18 and KR95 in serological diagnosis was evaluated and compared with rK28 and rK39. The results showed that rKR95 had higher sensitivity and specificity in the diagnosis of visceral leishmaniasis, suggesting its use in serological assays.
Article
Immunology
Mariane V. Monfardini, Renata T. Souza, Thais C. G. Rojas, Caroline G. Guerrieri, Cristina Orikaza, Isabel C. A. Scaletsky
Summary: Twenty-two atypical enteroaggregative Escherichia coli isolates from a previous epidemiological study were examined for their adhesion properties. Different patterns of aggregative adherence (AA) were observed among the isolates, with some strains showing typical AA and others showing variant AA. Further analysis of one strain, Q015B, revealed a hemagglutinin-associated protein (orfHA) that was genetically related to a putative filamentous hemagglutinin. Mutating orfHA resulted in loss of adhesion and reduced virulence in a G. mellonella model.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Clinical Neurology
Guilherme Luiz Fernandes, Julia Ribeiro da Silva Vallim, Vania D'Almeida, Sergio Tufik, Monica Levy Andersen
Summary: Sleepiness is influenced by multiple factors including social jetlag and sleep variability. This study found that the effects of social jetlag on sleepiness may be mediated by sleep debt, highlighting the importance of circadian misalignment and sleep debt for sleep health on a population level.
JOURNAL OF SLEEP RESEARCH
(2023)
Article
Immunology
Alice Maria Melo do Nascimento, Carolina Menezes Suassuna de Souza, Ana Claudia Dumont Oliveira, Maiara Garcia Blagitz, Eduardo Milton Ramos Sanchez, Alice Maria Melville Paiva Della Libera, Ricardo de Miranda Henriques Leite, Artur Cezar de Carvalho Fernandes, Fernando Nogueira Souza
Summary: This study explored the impact of BLV infection in the periparturient period on the expression of PD-1 and CTLA-4 in blood T cells, as well as the rate of new intramammary infections during early lactation. The results showed that BLV-infected dairy cows had higher expression of CTLA-4 and PD-1 in blood T cells, and a higher rate of new intramammary infections during early lactation.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2023)
Article
Microbiology
Guilherme S. de Moura, Eneas de Carvalho, Eduardo M. Ramos Sanchez, Fabio P. Sellera, Michele F. S. Marques, Marcos B. Heinemann, Sarne De Vliegher, Fernando N. Souza, Rinaldo A. Mota
Summary: The discovery of mecA and mecC-positive mammaliicocci from American continent represents a new reservoir of antimicrobial resistance genes, indicating a possible emergence of M. sciuri as a globally important pathogen. These M. sciuri strains carried a wide range of antimicrobial resistances, virulence-associated genes, and belonged to a globally disseminated branch associated with farm animals, companion animals, and food. The findings highlight the need to monitor M. sciuri under the One Health approach due to its potential spread at the human-animal-environment interface.
VETERINARY MICROBIOLOGY
(2023)
Article
Microbiology
Bruna Rocha Almeida, Bianca Carla Silva Campitelli Barros, Debora Tereza Lucas Barros, Cristina Mary Orikaza, Erika Suzuki
Summary: Studying the interaction between pathogens and hosts is crucial for understanding the establishment, maintenance, and spread of infections. This research shows that P. brasiliensis infection can increase the expression of alpha 3 integrin in late endosomes of lung epithelial cells, while also promoting its degradation through the host cell's endolysosomal pathway.
Article
Agriculture, Multidisciplinary
Carolina Menezes Suassuna de Souza, Maiara Garcia Blagitz, Fernando Nogueira Souza, Camila Freitas Batista, Daniel Magalhaes Lima, Alexandre Jose Alves, Marcos Bryan Heinemann, Eduardo Milton Ramos Sanchez, Hugo Frias Torres, Artur Cezar de Carvalho Fernandes, Alice Maria Melville Paiva Della Libera
Summary: This study investigated the predictive values of milk leukocyte differentials (MLD) for improving the diagnosis of intramammary infections (IMIs) and subclinical mastitis. The results showed that the combination of T CD8+ lymphocytes with PMNLs and macrophages can better diagnose bovine IMIs.