Article
Biochemistry & Molecular Biology
Priyadarshini Kachroo, Isobel D. Stewart, Rachel S. Kelly, Meryl Stav, Kevin Mendez, Amber Dahlin, Djora I. Soeteman, Su H. Chu, Mengna Huang, Margaret Cote, Hanna M. Knilhtila, Kathleen Lee-Sarwar, Michael McGeachie, Alberta Wang, Ann Chen Wu, Yamini Virkud, Pei Zhang, Nicholas J. Wareham, Elizabeth W. Karlson, Craig E. Wheelock, Clary Clish, Scott T. Weiss, Claudia Langenberg, Jessica A. Lasky-Su
Summary: The application of large-scale metabolomic profiling provides new opportunities for omics-based precision medicine in treating asthma. This study identifies and replicates 17 steroid metabolites that are significantly reduced in individuals with asthma, especially those receiving inhaled corticosteroid (ICS) treatment. The dose-dependent effect of ICS treatment on steroid levels suggests the need for regular cortisol monitoring to minimize the adverse effects of adrenal suppression. The findings highlight the importance of considering the public health implications of adrenal suppression in asthma patients receiving ICS treatment.
Article
Medicine, Research & Experimental
Chao Chen, Guixue Hou, Chunwei Zeng, Yan Ren, Xiang Chen, Cong Peng
Summary: The study revealed significant alterations in the metabolism of amino acids and carnitines in patients with psoriasis. Particularly, the metabolism of essential amino acids, branched-chain amino acids, and L-carnitine may play key roles in the pathogenesis of psoriasis.
Article
Biochemical Research Methods
Yingying Shi, Daling Ding, Liwei Liu, Zhuolun Li, Lihua Zuo, Lin Zhou, Quzheng Du, Ziwei Jing, Xiaojian Zhang, Zhi Sun
Summary: This study identified metabolic signatures in blood and urine associated with glioma, linking them to gene expression and pathways related to the disease. Specific changes in gene expression and correlation with metabolites were revealed, indicating a potential target for developing effective therapies. Integration of metabolomics with transcriptomics provided valuable insights into the molecular perturbations underlying glioma.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Engineering, Environmental
Yanping Zhao, Ruxia Qiao, Siyuan Zhang, Guoxiang Wang
Summary: The study found that microplastic fibers can cause intestinal damage and toxicity in zebrafish, with longer fibers producing more serious effects. Metabolomics analysis revealed that microplastic fibers can up-regulate glycerophospholipids metabolism, exacerbating oxidative damage and inflammation, while down-regulating fatty acyls metabolism related to nutritional deficiency.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Food Science & Technology
Min Jeong Kang, Keup-Rae Kim, Keono Kim, Aria G. Morrill, Chuleui Jung, Sukjun Sun, Dong-Hee Lee, Joon Hyuk Suh, Jeehye Sung
Summary: This study used metabolomics and sensory analysis to investigate the relationship between chemical profile and sensory quality of honey. The results showed that sweetness had a positive impact on consumer acceptance, while sourness, astringency, and bitterness had a negative impact. Consumers preferred honey with floral notes, but disliked those with off-flavors. The familiarity of flavor was strongly correlated with consumer acceptance, indicating the importance of balancing volatiles and non-volatiles for honey flavor quality.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Shufeng Wang, Xin Yang, Feng Liu, Xinzheng Wang, Xuemin Zhang, Kun He, Hongxia Wang
Summary: A comprehensive non-targeted metabolomic study on Hep3B cells exposed to AFB1 revealed dynamic metabolic reprogramming, including pathways such as purine and pyrimidine metabolism, hexosamine pathway and sialylation. The metabolomic strategy employed in this study showed high sensitivity and resolution, providing new insights into potential drug targets for therapeutic intervention in carcinogen-induced cancer.
Article
Plant Sciences
Kangning Zhang, He Li, Tian Zhang, Shixing Wang, Liang Liu, Xuyan Dong, Lili Cong, Hui Song, Aihua Wang, Guofeng Yang, Hongli Xie, Zeng-Yu Wang, Maofeng Chai
Summary: In this study, metabolomic and transcriptomic analyses were used to compare the nutrient composition of yellow and green alfalfa sprouts during germination. The results showed that green alfalfa sprouts contained a wide variety of lipids, flavonoids, phenolic acids, and terpenoids, while yellow alfalfa sprouts contained relatively more isoquercitrin.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Hematology
Fengjiao Wang, Jiahuan He, Siqi Liu, Ai Gao, Liu Yang, Guohuan Sun, Wanqiu Ding, Chuan-Yun Li, Fanglin Gou, Manman He, Fang Wang, Xiaoshuang Wang, Xiangnan Zhao, Ping Zhu, Sha Hao, Yanni Ma, Hui Cheng, Jia Yu, Tao Cheng
Summary: By analyzing 12 murine adult hematopoietic cell populations, this study identified 30796 editing sites and revealed the dynamic changes of the RNA editome during hematopoietic development. The highly edited Azin1 in HSPCs plays a crucial role in promoting cell differentiation.
Article
Plant Sciences
Zheng-He Lin, Chang-Song Chen, Qiu-Sheng Zhong, Qi-Chun Ruan, Zhi-Hui Chen, Xiao-Mei You, Rui-Yang Shan, Xin-Lei Li
Summary: This study highlights the different effects of N deficiency on the metabolism of tea leaves and roots. N-deficient tea leaves showed chlorosis, reduced biomass accumulation, and altered metabolite concentrations, while N-deficient tea roots exhibited increased accumulation of certain substances. N deficiency may lead to lower tea quality in leaves, but improved stress response in roots.
Article
Biology
Guilin Liu, Hongxiang Wang, Zhihua Lv, Xiaoqian Tang, Mingming Yu
Summary: This study investigated the changes in endogenous compounds of flounder at different temperatures using metabolomic and lipidomic analysis, and identified potential serum biomarkers and metabolic pathways.
JOURNAL OF THERMAL BIOLOGY
(2022)
Article
Plant Sciences
Pushpa Doddaraju, Prathibha M. Dharmappa, Aarthy Thiagarayaselvam, Preethi Vijayaraghavareddy, Raju Bheemanahalli, Priyanka A. Basavaraddi, Mohan Kumar V. Malagondanahalli, Sumanth Kambalimath, Hirekodathakallu V. Thulasiram, Sheshshayee M. Sreeman
Summary: A study identified the genetic variability in acquired tolerance traits (ATTs) among rice germplasm accessions and revealed their importance in protecting growth and productivity under water-limited conditions. Through various approaches including physiological screens, association mapping, and metabolomics, the study found significant marker-trait associations and discovered tolerance-related metabolites that are crucial for cellular functions and photosynthetic capacity. The research also identified genes controlling metabolic pathways related to osmolites and antioxidants, providing potential for translational crop improvement activities.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Cell Biology
Yoshito Kadota, Asuka Yano, Takashige Kawakami, Masao Sato, Shinya Suzuki
Summary: Metallothionein (MT) deficiency in mice led to higher levels of metabolic intermediate TML and lower mRNA levels of TML dioxygenase in the liver and kidney, suggesting reduced capacity for carnitine biosynthesis and potential metabolic disorders in mitochondria, which may contribute to shortened lifespans in MT knockout mice compared to wild-type mice.
Article
Biochemistry & Molecular Biology
Zhe Feng, Shuyu Ji, Di Cui
Summary: This study investigated the effect of Fe deficiency on metabolite synthesis and gene expression in leaves of G. bicolor. Fe deficiency led to chlorosis and decreased chlorophyll content in young leaves, while it had little effect on mature leaves.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Sehoon Park, Jueun Lee, Seung Hee Yang, Hajeong Lee, Joo Young Kim, Minkyoung Park, Kyu Hong Kim, Jong Joo Moon, Semin Cho, Soojin Lee, Yaerim Kim, Jung Pyo Lee, Chang Wook Jeong, Cheol Kwak, Kwon Wook Joo, Chun Soo Lim, Yon Su Kim, Geum-Sook Hwang, Dong Ki Kim
Summary: The study identified urinary glycine as a potential biomarker for the diagnosis and prognosis of IgAN. Higher levels of urinary glycine were associated with a lower risk of kidney function decline in IgAN patients. Experimental validation in human kidney tubular epithelial cells showed that glycine could ameliorate inflammatory signals induced by tumor necrosis factor-alpha.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Biochemical Research Methods
Na Guo, Yangan Chen, Xiaofang Yang, Han Yan, Bin Fan, Jianye Quan, Mei Wang, Hongjun Yang
Summary: This study used untargeted and targeted metabolomics approaches to explore the significantly different metabolites related to the Qi stagnation with blood stasis or Qi deficiency with blood stasis subtypes of coronary heart disease (CHD) with angina pectoris. The results showed distinct metabolic profiles not only between CHD patients and healthy controls, but also between the two subtypes of CHD. Pathway analysis revealed subtype-related differences in the activity of pathways, demonstrating the potential of urinary metabolomics in providing evidence for personalized treatment of CHD.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ryota Watano, Tsukasa Ohmori, Shuji Hishikawa, Asuka Sakata, Hiroaki Mizukami
Article
Neurosciences
Akira Arafune-Mishima, Hiroshi Abe, Toshiki Tani, Hiromi Mashiko, Satoshi Watanabe, Kazuhisa Sakai, Wataru Suzuki, Hiroaki Mizukami, Akiya Watakabe, Tetsuo Yamamori, Noritaka Ichinohe
Article
Multidisciplinary Sciences
Machi Horiai, Ayano Otsuka, Shizu Hidema, Yuichi Hiraoka, Ryotaro Hayashi, Shinji Miyazaki, Tamio Furuse, Hiroaki Mizukami, Ryoichi Teruyama, Masaru Tamura, Haruhiko Bito, Yuko Maejima, Kenju Shimomura, Katsuhiko Nishimori
SCIENTIFIC REPORTS
(2020)
Article
Oncology
Takahiro Koyanagi, Yasushi Saga, Yoshifumi Takahashi, Kohei Tamura, Takahiro Yoshiba, Suzuyo Takahashi, Akiyo Taneichi, Yuji Takei, Masashi Urabe, Hiroaki Mizukami, Hiroyuki Fujiwara
Summary: VASH2 knockout in ovarian cancer cells enhances chemosensitivity to paclitaxel by reducing tubulin detyrosination and upregulating cyclin B1 expression. This study suggests that targeting VASH2 may be a promising strategy for ovarian cancer treatment by inhibiting angiogenesis and modulating microtubule activity.
Article
Biochemistry & Molecular Biology
Mari Isomi, Taketaro Sadahiro, Ryo Fujita, Yuto Abe, Yu Yamada, Tatsuya Akiyama, Hiroaki Mizukami, Tsugumine Shu, Keiichi Fukuda, Masaki Ieda
Summary: Adult hearts have limited regenerative capacity, but direct reprogramming of cardiac fibroblasts into induced cardiomyocytes with GMT may have significant potential for cardiac repair. Studies have shown that SeV-GMT reprogramming in mouse hearts after acute MI can improve cardiac function.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Letter
Cardiac & Cardiovascular Systems
Mari Isomi, Taketaro Sadahiro, Hiroyuki Yamakawa, Ryo Fujita, Yu Yamada, Yuto Abe, Yoshiko Murakata, Tatsuya Akiyama, Tsugumine Shu, Hiroaki Mizukami, Keiichi Fukuda, Masaki Ieda
Article
Biotechnology & Applied Microbiology
Yoshihide Sehara, Yuka Hayashi, Kenji Ohba, Ryosuke Uchibori, Masashi Urabe, Ayumu Inutsuka, Kuniko Shimazaki, Kensuke Kawai, Hiroaki Mizukami
Summary: This study compared the transduction efficiency of AAV2, 5, and rh10 in the hippocampal DG, finding that AAV5 exhibited a higher efficiency in neural stem cells and precursor cells, while AAVrh10 showed a greater efficiency in mature neurons.
HUMAN GENE THERAPY
(2022)
Article
Endocrinology & Metabolism
Naoki Usui, Masahide Yoshida, Yuki Takayanagi, Naranbat Nasanbuyan, Ayumu Inutsuka, Hiroshi Kurosu, Hiroaki Mizukami, Yoshiyuki Mori, Makoto Kuro-o, Tatsushi Onaka
Summary: FGF21 plays a role in modulating energy metabolism, neuroendocrine stress responses, and controlling behavioral changes in male rodents under social defeat stress conditions. The study suggests that endogenous FGF21 may have an inhibitory effect on stress-induced depression-like behavior in male rodents.
JOURNAL OF NEUROENDOCRINOLOGY
(2021)
Article
Multidisciplinary Sciences
Rintaro Kuroda, Kaoru Tominaga, Katsumi Kasashima, Kenji Kuroiwa, Eiji Sakashita, Hiroko Hayakawa, Tom Kouki, Nobuhiko Ohno, Kensuke Kawai, Hitoshi Endo
Summary: Mitochondrial dysfunction caused by Tfam inactivation in neural stem cells impairs respiratory chain activities, leading to deficits in neural differentiation and maturation in both in vivo and in vitro settings. This dysfunction triggers an integrated stress response that may protect against neurodegenerative diseases.
Article
Immunology
Mohammad Shahnaij, Mitsuhiro Iyori, Hiroaki Mizukami, Mayu Kajino, Iroha Yamagoshi, Intan Syafira, Yenni Yusuf, Ken Fujiwara, Daisuke S. Yamamoto, Hirotomo Kato, Nobuhiko Ohno, Shigeto Yoshida
Summary: By using AAV8 as a booster, the immune responses in mice were improved, providing better protection against malaria and demonstrating great promise as a next-generation platform for effective malaria vaccine development.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Nemekhbayar Baatartsogt, Yuji Kashiwakura, Morisada Hayakawa, Nobuhiko Kamoshita, Takafumi Hiramoto, Hiroaki Mizukami, Tsukasa Ohmori
Summary: A reliable and sensitive cell-based assay to measure neutralizing antibodies against AAV was developed with a detection threshold of 100. High-dose AAV8 vector treatment enabled evasion of the inhibitory effect of neutralizing antibodies, while low-dose treatment may have slight influence even when antibodies are negative in the assay.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Yasuyuki Osanai, Batpurev Battulga, Reiji Yamazaki, Tom Kouki, Megumi Yatabe, Hiroaki Mizukami, Kenta Kobayashi, Yoshiaki Shinohara, Yumiko Yoshimura, Nobuhiko Ohno
Summary: An appropriate sensory experience during the early developmental period is crucial for brain maturation. Dark rearing during the visual critical period affects the structure and morphology of myelin sheaths in the visual pathway, resulting in thicker and shorter sheaths.
NEUROCHEMICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Kaoru Tominaga, Eiji Sakashita, Katsumi Kasashima, Kenji Kuroiwa, Yasumitsu Nagao, Naoki Iwamori, Hitoshi Endo
Summary: Epigenetic factors play a crucial role in establishing functional organ systems during development, including brain development. However, the precise mechanism of epigenetic regulation in brain development and neurogenesis remains largely unknown. In this study, the researchers discovered the critical role of the Tip60 protein in brain development and the maintenance and function of neural stem/progenitor cells (NSCs). Tip60 deficiency led to multiple abnormalities in brain development, including microcephaly, reduced proliferation of developing brain cells, and impaired neural differentiation and migration. The study also revealed that Tip60 is involved in the transition from neurogenesis to gliogenesis during brain development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Toshimitsu Suzuki, Satoko Hattori, Hiroaki Mizukami, Ryuichi Nakajima, Yurina Hibi, Saho Kato, Mahoro Matsuzaki, Ryu Ikebe, Tsuyoshi Miyakawa, Kazuhiro Yamakawa
Summary: This study found that deletion of the Scn2a gene in schizophrenia-related brain regions (medial prefrontal cortex and ventral tegmental area) in mice resulted in schizophrenia-relevant behavioral abnormalities. These findings are important for understanding the pathological mechanisms of schizophrenia.
MOLECULAR NEUROBIOLOGY
(2023)
Meeting Abstract
Biotechnology & Applied Microbiology
Sachie Nakamura, Hitoshi Osaka, Shin-ichi Muramatsu, Naomi Takino, Mika Ito, Eriko F. Jimbo, Hiroaki Mizukami, Shuji Hishikawa, Takeshi Nakajima, Takanori Yamagata
