Article
Biophysics
Menglin Song, Man-Chung Wong, Lihua Li, Feng Guo, Yuan Liu, Yingjing Ma, Xinyue Lao, Pui Wang, Honglin Chen, Mo Yang, Jianhua Hao
Summary: We have developed a point-of-care upconversion luminescence diagnostics (PULD) method combined with a streamlined smartphone-based portable platform for rapid testing of the SARS-CoV-2 nucleocapsid (N) gene. This method demonstrates high sensitivity and signal-to-noise ratio, and can accurately identify positive COVID-19 samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Multidisciplinary
Wenpeng Liu, Luke P. P. Lee
Summary: The global outbreaks of infectious diseases have led to a significant demand for rapid and accurate molecular diagnostics. However, the labor-intensive sample preparation and nucleic acid amplification steps make it challenging to carry out these diagnostics at home. This review discusses the advances and challenges in automatic solutions for sample preparation and on-chip nucleic acid amplification, as well as the potential for game-changing user-friendly approaches for next-generation home diagnostics.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
David S. Kinnamon, Jacob T. Heggestad, Jason Liu, Thu Nguyen, Vo Ly, Angus M. Hucknall, Cassio M. Fontes, Rhett J. Britton, Jian-Piao Cai, Jasper Fuk-Woo Chan, Kwok-Yung Yuen, Thuy Le, Ashutosh Chilkoti
Summary: We developed a self-contained immunoassay platform that can be used in field settings with varied temperature, humidity, and blood samples. The platform has high sensitivity and accuracy in detecting analyte concentration, and a portable detection device. We also improved the microfluidic cassette for better usability and faster results. By using this platform, we successfully conducted a rapid diagnostic test for talaromycosis infection in patients, showing comparable sensitivity and specificity to laboratory tests.
Article
Virology
Eline Meyers, Jeongho Park, Anja Coen, Leen Raman, Stefan Heytens, Joowon Rhee, Elizaveta Padalko, Piet Cools
Summary: During the SARS-CoV-2 pandemic, rapid and sensitive detection of the virus is crucial. A new point-of-care test system based on LAMP technology was evaluated, and it showed high clinical sensitivity and specificity for SARS-CoV-2 detection. The system's easy operation and ready-to-use reagents make it suitable for various settings, contributing to more efficient outbreak control and quarantine policies.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Immunology
Angelica F. Tan, Sitti Saimah Binti Sakam, Giri S. Rajahram, Timothy William, Mohammad Faruq Abd Rachman Isnadi, Sylvia Daim, Bridget E. Barber, Steven Kho, Colin J. Sutherland, Nicholas M. Anstey, Seda Yerlikaya, Donelly A. van Schalkwyk, Matthew J. Grigg
Summary: The study evaluated ten rapid diagnostic tests for detection of P. knowlesi, with some tests showing sufficient performance for detecting major human malaria species (including P. knowlesi) in co-endemic areas, particularly for higher parasite counts. However, these tests cannot reliably differentiate non-falciparum malaria.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Meng Xiao, Feng Tian, Xin Liu, Qiaoqiao Zhou, Jiangfei Pan, Zhaofan Luo, Mo Yang, Changqing Yi
Summary: This article reviews the latest developments in virus detection, comparing laboratory-based and smartphone-based diagnostic technologies, discussing various smartphone-based POCT diagnostic techniques, and showcasing diagnostic methods for various viruses such as COVID-19. Additionally, it explores the prospects and challenges of smartphone-based POCT diagnostics.
Article
Biochemical Research Methods
Dana L. Cortade, Shan X. Wang
Summary: This article presents a method for quantitative, sensitive, and rapid detection of low-cost opioids using giant magnetoresistive (GMR) nanosensors in a clinical setting. They validated the method in saliva, blood, and plasma, and demonstrated a fully automated platform with efficient result transmission via smartphone and Bluetooth.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Medicine, General & Internal
Darius Riziki Martin, Nicole Remaliah Sibuyi, Phumuzile Dube, Adewale Oluwaseun Fadaka, Ruben Cloete, Martin Onani, Abram Madimabe Madiehe, Mervin Meyer
Summary: The transmission of Tuberculosis is rapid and impacts healthcare systems globally. Current diagnostic methods are time-consuming and costly. By utilizing aptamers instead of antibodies, new rapid tests can be developed that are more cost-effective, stable, and reliable.
Review
Medical Laboratory Technology
Anupam Jyoti, Sanni Kumar, Vijay Kumar Srivastava, Sanket Kaushik, Shiv Govind Singh
Summary: Sepsis in neonates, caused by infection and inflammation, is a leading cause of death worldwide. Early diagnosis and comprehensive evaluation are crucial for effective clinical management. The advancement in biosensing technology offers potential for improved diagnostic methods in septic neonates.
CLINICA CHIMICA ACTA
(2021)
Review
Microbiology
Madeleine J. Rogers, Donald P. McManus, Stephen Muhi, Catherine A. Gordon
Summary: Parasitic neglected tropical diseases affect over one billion people worldwide, especially those in low-socioeconomic areas. The lack of infrastructure and sensitive, affordable diagnostic methods hinder disease management programs. Membrane technology offers a solution for simplifying sample storage and diagnostic procedures, although cost remains a barrier for accessibility in endemic regions. New, simple, rapid, accurate, and cost-effective immunological procedures and nucleic acid diagnostics protocols are necessary for progressing control efforts towards elimination of these diseases.
CLINICAL MICROBIOLOGY REVIEWS
(2021)
Article
Biophysics
Qiangqiang Fu, Tao Qi, Ze Wu, Yongjian He, Shujuan Guan, Shihua Luo, Qi Zhang, Wenfeng Luo, Wei Xiao, Bo Situ, Lei Zheng
Summary: This study presents a portable and affordable smartphone-based Hb analyzer for quick anemia testing, especially suitable for high-risk patients in areas with limited medical resources.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Engineering, Biomedical
Ashna Gopal, Li Yan, Saima Kashif, Tasnim Munshi, Vellaisamy A. L. Roy, Nicolas H. Voelcker, Xianfeng Chen
Summary: The rise in antimicrobial resistance has led to a crucial need to optimize infection therapy approaches, with rapid and effective identification of pathogenic bacteria being a key stage. Traditional bacterial detection techniques have downsides, but innovative technologies with high detection sensitivity and specificity are being developed to overcome these limitations.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biophysics
Qurat ul Ain Zahra, Syed Agha Hassnain Mohsan, Faisal Shahzad, Mohammad Qamar, Bensheng Qiu, Zhaofeng Luo, Shabi Abbas Zaidi
Summary: Conventional analytical biosensor devices have limitations in handling, time, size, and cost. Smartphones coupled with aptamers have enabled the development of portable and cost-effective biosensors for biomolecule detection. This review discusses achievements in smartphone-enabled aptasensors between 2017 and 2022, covering fabrication strategies and software. The report aims to assist scientists and engineers in understanding smartphone-based aptasensor fabrication and encourage future developments in affordable and portable sensing devices.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Medical Laboratory Technology
Vinoth Kumar Rajendran, Padmavathy Bakthavathsalam, Peter L. Bergquist, Anwar Sunna
Summary: Reliable detection of nucleic acids at low concentrations in clinical samples and food can be achieved by nucleic acid amplification methods. The use of smartphones for nucleic acid testing has shown promising progress in endpoint and real-time analysis of various disease conditions.
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
(2021)
Article
Biochemical Research Methods
Iida Martiskainen, Sheikh M. Talha, Karoliina Vuorenpaa, Teppo Salminen, Etvi Juntunen, Souvick Chattopadhyay, Dinesh Kumar, Tytti Vuorinen, Kim Pettersson, Navin Khanna, Gaurav Batra
Summary: A highly sensitive rapid diagnostic test for hepatitis B Virus surface antigen (HBsAg) was developed in this study, which can meet the needs for patients with low levels of HBsAg and blood screening. UCNP-LFIA showed higher sensitivity and accuracy in sample detection, making it a reliable detection method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Pediatrics
Lilly-Ann Mohlkert, Gunnar Sjoberg, Annika Rydberg, Cecilia Pegelow Halvorsen, Ellen Tufvesson, Jenny Hallberg, Magnus Domellof, Mikael Norman
Article
Pediatrics
Nhung T. H. Pham, Nhan D. T. Le, Ngai K. Le, Khoa D. Nguyen, Mattias Larsson, Linus Olson, Dien M. Tran
Article
Pediatrics
Hang T. T. Tran, Ha T. T. Le, Hanh T. P. Tran, Dung T. K. Khu, Hugo Lagercrantz, Dien M. Tran, Birger Winbladh, Lena Hellstrom-Westas, Tobias Alfven, Linus Olson
Summary: The study demonstrates that phase-changing material can be an effective method for cooling asphyxiated newborns, maintaining target temperature range and rewarming infants in a slow and controlled manner, with easy operation and minimal risks.
Article
Medicine, General & Internal
Lilly-Ann Mohlkert, Jenny Hallberg, Olof Broberg, Gunnar Sjoberg, Annika Rydberg, Petru Liuba, Vineta Fellman, Magnus Domellof, Mikael Norman, Cecilia Pegelow Halvorsen
Summary: This study found that children born extremely preterm at 6.5 years old exhibited important changes in the right heart structure and function, including smaller right atria, right ventricles, and higher estimated pulmonary vascular resistance. Preterm children with PDA had higher PVR and right ventricular myocardial performance index compared to those without PDA, suggesting an association between PDA and altered cardiac outcomes in extremely preterm children.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Pediatrics
Mattias Larsson, Hoa Quynh Nguyen, Linus Olson, Toan Khanh Tran, Trung Vu Nguyen, Chuc Thi Kim Nguyen
Summary: This study revealed a high prevalence (40%) of Streptococcus pneumoniae colonization among preschool children in rural Vietnam, with decreased susceptibility to antibiotics compared to previous years. Multi-drug resistance increased from 31% in 1999 to 80% in 2014.
Article
Infectious Diseases
Aislinn Cook, Mike Sharland, Yasmine Yau, Julia Bielicki
Summary: A WISCA is a practical tool for evaluating empiric antibiotic regimens for pediatric sepsis based on local microbiology data. The coverage estimates for third-generation cephalosporins and meropenem ranged widely across pediatric hospitals globally, demonstrating the need for individualized prescribing guidelines to address antibiotic resistance.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2022)
Article
Infectious Diseases
K. K. Sriram, Elina Ekedahl, Ngoc Thi Bich Hoang, Tsegaye Sewunet, Bjorn Berglund, Ludwig Lundberg, Shoeib Nematzadeh, Maud Nilsson, Lennart E. Nilsson, Ngai Kien Le, Dien Minh Tran, Hakan Hanberger, Linus Olson, Mattias Larsson, Christian G. Giske, Fredrik Westerlund
Summary: The diversity of bla(NDM-1)-encoding plasmids from neonates at a large Vietnamese hospital was characterized using optical DNA mapping (ODM) and next-generation sequencing (NGS). The results showed that there were at least 10 different plasmids encoding bla(NDM-1) among the 18 isolates, indicating wide plasmid diversity. This study highlights the importance of ODM for plasmid analysis.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Chemistry, Physical
Linus Olson, Carina Lothian, Ulrika aden, Hugo Lagercrantz, Nicola J. Robertson, Fredrik Setterwall
Summary: Research on utilizing Glauber salt solution as a suitable phase change material for cooling infants with asphyxia was conducted through empirical experiments studying different mixtures of NaCl, Na2SO4, and water. This study aimed to better understand how these mixtures act at temperatures ranging from 28 to 40 degrees Celsius and to compose salt solutions that meet clinical cooling specifications.
Article
Pediatrics
Tina Dempsey, Huong Lien Nguyen, Huong Thu Nguyen, Xuan Anh Bui, Phuong Thi Thu Pham, Toan K. Nguyen, Francesco Cavallin, Daniele Trevisanuto, Susanna Myrnerts Hook, Nicolas Pejovic, Mats Blennow, Linus Olson, Hien Vu, Anh Duy Nguyen, Tobias Alfven
Summary: Every year, a large number of neonates die or suffer injuries during their first month of life, mainly due to intrapartum-related events, prematurity, and infection. This study focused on neonates born at the largest obstetric hospital in Hanoi, Vietnam, and found that while other indicators were consistent with previous data, the number of intrapartum-related events was surprisingly low, even compared to high-income countries. Further research on the current clinical practices at Phu San Hanoi Hospital may help identify the factors contributing to this success.
Article
Multidisciplinary Sciences
Tsegaye Sewunet, K. K. Sriram, Ha Hoang Nguyen, Noikaseumsy Sithivong, Ngoc Thi Bich Hoang, Vanphanom Sychareun, Kokasia Nengmongvang, Mattias Larsson, Linus Olson, Fredrik Westerlund, Christian G. Giske
Summary: In this study, the colonization rate of carbapenemase-producing Enterobacterales (CPE) on admission to intensive care units in Vientiane, Lao PDR was determined. The prevalence of extended-spectrum beta-lactamases (ESBLs) was high, while carbapenemases were less common. However, clonal dissemination of bla(NDM-1)-producing Klebsiella pneumoniae isolates in one of the intensive care units was detected, highlighting the need for stringent infection prevention and antimicrobial stewardship strategies.
Article
Infectious Diseases
Amy Riddell, Aislinn Cook, Nathalie Khavessian, Sally Ellis, Davide Bilardi, Erika Correia, Tomislav Kostyanev, Alessandra Nardone, Neal Russell, Tuba Vilken, Wolfgang Stohr, Bethou Adhisivam, Iana Rosa Alves de Moraes, Nawshad Uddin Ahmed, Adrie Bekker, Eitan Naaman Berezin, Suppawat Boonkasidecha, Cristina G. Carvalheiro, Prachi Chauhan, Sara Chiurchiu, Elisavet Chorafa, Angela Dramowski, D. S. Madhusudhan, Jinxing Feng, Shengnan Jia, Yuan Kong, Mary Kyohere, Angeliki Kontou, Sorasak Lochindarat, Maia De Luca, Aripfani Mphaphuli, Marisa M. Mussi-Pinhata, Sheila Murunga, Firdose Lambey Nakwa, Sushma Nangia, Erinah Nassolo, Ngoc Thi Bin Hoang, Christina W. Obiero, Linus Olson, Wang Ping, Nishad Plakkal, Priyanka Prasad, Kanchana Preedisripipat, Sheikh Wasik Rahman, Tiffany Seef, Pra-ornsuda Sukrakanchana, Reenu Thomas, Zhang Yu, Qiaoru Zhang, A. Sarah Walker, Julia Bielicki, Paul T. Heath, Michael Sharland, Tatiana Munera-Huertas
Summary: Neonatal sepsis is a major problem in low- and middle-income countries, and understanding the challenges of managing global multi-center research studies is crucial for obtaining high-quality data. This paper discusses the complexities faced by diverse research teams in different countries, and presents actions taken to overcome these challenges in a large observational study of neonatal sepsis. Flexibility in recruitment strategies, ongoing training, and meticulous design and monitoring of the database were key to success. The importance of a strong central management team and interdisciplinary collaborators was also emphasized.
Article
Critical Care Medicine
Tina Dempsey, Huong Thu Nguyen, Huong Lien Nguyen, Xuan Anh Bui, Phuong Thi Thu Pham, Toan K. Nguyen, Daniel Hellden, Francesco Cavallin, Daniele Trevisanuto, Susanna Myrnerts Hook, Mats Blennow, Linus Olson, Hien Vu, Anh Duy Nguyen, Tobias Alfven, Nicolas Pejovic
Summary: This study conducted at Phu San Hanoi Hospital in Vietnam found that incidences of positive pressure ventilation and endotracheal intubation were low in comparison to global estimates. Three out of four intubations were successful at the first attempt, with a median first attempt duration longer than recommended. Future studies should focus on achieving and maintaining intubation skills, and consider alternative devices for airway management at birth.
RESUSCITATION PLUS
(2022)
Article
Infectious Diseases
Nguyen Thi Phuong Yen, Nguyen Thi Nhung, Doan Hoang Phu, Nguyen Thi Thuy Dung, Nguyen Thi Bich Van, Bach Tuan Kiet, Vo Be Hien, Mattias Larsson, Linus Olson, James Campbell, Nguyen Pham Nhu Quynh, Pham Thanh Duy, Juan Carrique-Mas
Summary: Vietnam and Southeast Asia are hotspots for antimicrobial resistance and the prevalence of carbapenem-resistant Enterobacteriaceae (CRE), particularly Escherichia coli (CREC) and Klebsiella pneumoniae (CRKP) is emerging. The study found that in the Mekong Delta of Vietnam, CRE isolates were present in human rectal swabs (0.6%), pigs (0.7%), chickens (2.1%), and ducks (0.7%). The presence of CRE was higher among individuals who had used antimicrobials recently. All CRE/CRAB strains were multidrug resistant, but susceptible to some antibiotics. The identification of carbapenemase genes raises concerns about potential transmission of resistant organisms between healthcare and community settings.
JAC-ANTIMICROBIAL RESISTANCE
(2022)
Article
Microbiology
Thanh C. Tran, Binh T. Pham, Van H. Pham, The A. Ngo, Hakan Hanberger, Mattias Larsson, Linus Olson
Article
Infectious Diseases
Linh Vu Phuong Dang, Hung Viet Pham, Thanh Thi Dinh, Phuong Thi Vu, Lam Van Nguyen, Hai Thanh Le, Mattias Larsson, Linus Olson
THERAPEUTIC ADVANCES IN INFECTIOUS DISEASE
(2020)
