Review
Immunology
Kelin Zhao, Chenfei Kong, Naixu Shi, Jinlan Jiang, Ping Li
Summary: This review summarizes the therapeutic effects of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) on systemic sclerosis (SSc) and the mechanisms, providing a theoretical basis for future research on the role of MSC-EVs in treating SSc.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Physical
Chunhui Sheng, Xiaodong Guo, Zhuqing Wan, Xiaoqiang Bai, Hao Liu, Xiao Zhang, Ping Zhang, Yunsong Liu, Wenyue Li, Yongsheng Zhou, Longwei Lv
Summary: This study investigated the potential of exosomes derived from human adipose-derived stem cells (hASCs) in promoting osteogenic differentiation for osteoporosis treatment. The study identified the key differentially expressed microRNA between two exosomes and their potential target gene. Results showed that exosomes from osteogenic induction medium had a better effect on promoting osteogenic differentiation than those from proliferation medium. Moreover, miR-335-3p inhibitor-optimized exosomes enhanced osteogenic differentiation in vitro and bone density in vivo, suggesting the potential of engineered exosomes in optimizing exosome-based therapy for osteoporosis.
Article
Dermatology
Hritu Baral, Akihiko Uchiyama, Yoko Yokoyama, Akiko Sekiguchi, Sahori Yamazaki, Syahla Nisaa Amalia, Yuta Inoue, Sachiko Ogino, Ryoko Torii, Mari Hosoi, Toshiyuki Matsuzaki, Sei-ichiro Motegi
Summary: The study demonstrated that MSCs-derived exosomes inhibited dermal fibrosis in a bleomycin-induced SSc mouse model, potentially through the suppression of collagen type I expression by miR-196b-5p present in the exosomes.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2021)
Article
Engineering, Biomedical
Min Nie, Bin Kong, Guopu Chen, Ying Xie, Yuanjin Zhao, Lingyun Sun
Summary: The novel MSCs-laden injectable self-healing hydrogel developed for SSc treatment shows promising results in promoting MSCs proliferation, increasing cellular half-life, promoting angiopoiesis, and inhibiting fibrosis in disease model mice.
BIOACTIVE MATERIALS
(2022)
Review
Cell Biology
Mehrdad Hajinejad, Sajad Sahab-Negah
Summary: Neuroinflammation plays a crucial role in neurological disorders, and mesenchymal stem cells can modulate inflammation and microglia/macrophage polarization through exosome secretion. Studies have shown that miRNAs contained in exosomes have the potential to impact recipient cell behavior, providing a novel therapeutic approach for addressing inflammatory pathways in neurological diseases.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lihu Xie, Xiaoping Long, Meili Mo, Jinmei Jiang, Qingxiu Zhang, Mei Long, Mei Li
Summary: This study investigates the role of bone marrow mesenchymal stem cell-derived exosome (BMSC-Exos) delivering miR-214 in systemic sclerosis (SSc) and its relationship with the IL-33/ST2 axis.
MOLECULAR IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Lite Ge, Chengfeng Xun, Wenshui Li, Shengyu Jin, Zuo Liu, Yi Zhuo, Da Duan, Zhiping Hu, Ping Chen, Ming Lu
Summary: The study revealed that EVs from hypoxic OM-MSCs can enhance angiogenesis through the miR-612-TP53-HIF-1α-VEGF axis, suggesting a promising therapeutic strategy for ischemic diseases.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Review
Biology
Eloisa Romano, Irene Rosa, Bianca Saveria Fioretto, Marco Matucci-Cerinic, Mirko Manetti
Summary: In systemic sclerosis (SSc), abnormalities in microvessel morphology evolve into a distinctive vasculopathy that progresses alongside tissue fibrosis orchestrated by myofibroblasts. The endothelial-to-mesenchymal transition (EndoMT) process may play a central role in the pathogenesis of SSc, contributing to both fibrotic vascular lesions and tissue fibrosis. A deeper understanding of the mechanisms underlying myofibroblast differentiation inside vessel walls provides the basis for targeted therapeutic strategies for SSc.
Article
Biochemistry & Molecular Biology
Anna Wajda, Marcela Walczyk, Ewa Dudek, Barbara Stypinska, Aleksandra Lewandowska, Katarzyna Romanowska-Prochnicka, Marek Chojnowski, Marzena Olesinska, Agnieszka Paradowska-Gorycka
Summary: Systemic sclerosis (SSc) is a rare multisystem chronic disease with autoimmune features. In this study, the expression profile of selected microRNAs, including miR-126, -132, -143, -145, -155, -181a, -29a and -3148, was investigated in SSc patients compared to healthy controls. The results showed that the expression of these microRNAs was significantly altered in SSc patients, with miR-181a downregulated and miR-132, miR-143, miR-145 and miR-155 overexpressed. These findings suggest that these microRNAs may serve as potential biomarkers for SSc diagnosis and disease progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Mark Jakob, Mario Hambrecht, Jennifer L. Spiegel, Julia Kitz, Martin Canis, Ralf Dressel, Katrin Streckfuss-Bomeke
Summary: A multimodal therapeutic approach involving radiotherapy is essential for treating head and neck squamous cell carcinoma, but its use is limited due to the risk of damaging surrounding healthy tissue. Mesenchymal stem cells show promising therapeutic potential in cell-based tissue engineering and regenerative medicine due to their immunomodulatory properties. Generating induced pluripotent stem cell-derived mesenchymal stem cells in an autologous manner could be a novel personalized treatment option in regenerative medicine.
Article
Engineering, Biomedical
Zhaowei Yin, Chaoren Qin, Shaowei Pan, Chen Shi, Guanfu Wu, Yan Feng, Jing Zhang, Ziyi Yu, Bin Liang, Jianchao Gui
Summary: This study compared the therapeutic effects of exosomes derived from different sources of stem cells in osteoarthritis (OA) treatment. The results showed that exosomes derived from infrapatellar fat pad (IPFP) stem cells had a significantly stronger inhibitory effect on the degradation of cartilage extracellular matrix (ECM) compared to those derived from subcutaneous adipose tissue (ScAT) stem cells. Furthermore, microRNA sequencing revealed differences in microRNA expression between the two types of exosomes, and overexpression of a specific microRNA in ScAT-derived exosomes demonstrated efficacy in inhibiting the expression of ADAMTS4, promoting ECM repair in OA. Additionally, a hyaluronan-based hydrogel microparticles (HMPs) encapsulating exosomes were fabricated using microfluidic technology, and their injectability, sustained release, and long-term therapeutic effects on OA were validated.
MATERIALS TODAY BIO
(2023)
Article
Orthopedics
Tengjing Xu, Yunting Lin, Xinning Yu, Guangyao Jiang, Jiajie Wang, Kaiwang Xu, Jinghua Fang, Siheng Wang, Xuesong Dai
Summary: This study compared the effects of adipose stem cell-derived exosomes and ectosomes on Achilles tendinopathy. The results showed that ASC-Exos had more beneficial effects on tendon repair than ASC-Ectos.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2022)
Review
Engineering, Biomedical
Calvin Chang, Jerry Yan, Zhicheng Yao, Chi Zhang, Xiaowei Li, Hai-Quan Mao
Summary: This review focuses on the bioactivity and secretome of mesenchymal stem cells, as well as the engineering approaches to tune the MSC secretome. It also discusses the recent advances in biomaterials-based therapeutic strategies for delivering MSCs and MSC-derived secretome in extracellular vesicles, along with their advantages and challenges.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Cell & Tissue Engineering
Jiehan Cui, Lu Jin, Meng Ding, Jingjing He, Lin Yang, Shaoxin Cui, Xiaoping Wang, Jun Ma, Aijing Liu
Summary: This systematic review and meta-analysis found that mesenchymal stem cell (MSC) therapy can improve the degree of skin fibrosis, lung function, and mouth opening, and relieve finger ulcers and pain in patients with systemic sclerosis (SSc) without severe adverse events.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Medicine, Research & Experimental
Da-Won Choi, Kyung-Ah Cho, Jungwoo Kim, Hyun-Ji Lee, Yu-Hee Kim, Jang-Won Park, So-Youn Woo
Summary: Mesenchymal stem cells (MSCs) are adult stem cells originating from the mesoderm with multidirectional differentiation potential and immune modulation properties. They release extracellular vesicles (EVs) that play a role in tumor microenvironment communication. Tonsil-derived MSCs (T-MSCs) have been found to inhibit tumor growth through miR-199a-3p targeting CD151, integrin alpha 3 and 6 in liver cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Letter
Rheumatology
Piero Ruscitti, Giorgio Sesti, Paola Cipriani, Roberto Gerli, Roberto Giacomelli
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Rheumatology
Ilenia Di Cola, Federico Bruno, Onorina Berardicurti, Riccardo Monti, Alessandro Conforti, Alessandra Di Sibio, Viktoriya Pavlych, Carlo Masciocchi, Antonio Barile, Paola Cipriani, Piero Ruscitti
Summary: This study aimed to describe multivisceral involvement in adult-onset Still's disease (AOSD) and identify imaging-based differences in patients with and without macrophage activation syndrome (MAS). Patients with MAS had higher ferritin and systemic score values, as well as a higher prevalence of lung disease, hepatomegaly, splenomegaly, abdominal effusions, and lymph node enlargement. These manifestations correlated significantly with the systemic score, highlighting their prognostic value. Due to study limitations, further research is needed to confirm these findings and fully understand their implications.
CLINICAL RHEUMATOLOGY
(2021)
Letter
Rheumatology
Francesco Ursini, Piero Ruscitti, Vincenzo Raimondo, Rossella De Angelis, Fabio Cacciapaglia, Erika Pigatto, Domenico Olivo, Ilenia Di Cola, Felice Galluccio, Francesca Francioso, Rosario Foti, Antonio Tavoni, Salvatore D'Angelo, Corrado Campochiaro, Francesca Motta, Maria De Santis, Silvia Bilia, Caterina Bruno, Giacomo De Luca, Marcella Visentini, Jacopo Ciaffi, Luana Mancarella, Veronica Brusi, Martina D'Onghia, Giovanna Cuomo, Enrico Fusaro, Lorenzo Dagna, Serena Guiducci, Riccardo Meliconi, Florenzo Iannone, Annamaria Iagnocco, Roberto Giacomelli, Clodoveo Ferri
ANNALS OF THE RHEUMATIC DISEASES
(2022)
Article
Rheumatology
Piero Ruscitti, Ilenia Di Cola, Onorina Berardicurti, Alessandro Conforti, Daniela Iacono, Ilenia Pantano, Gelsomina Rozza, Silvia Rossi, Stefano De Ludovico, Silvia Balduzzi, Antonio Vitale, Francesco Caso, Luisa Costa, Marcella Prete, Luca Navarini, Fabiola Atzeni, Giuliana Guggino, Federico Perosa, Luca Cantarini, Bruno Frediani, Carlomaurizio Montecucco, Francesco Ciccia, Roberto Giacomelli, Paola Cipriani
Summary: This study found that smoking may affect the prognosis of adult-onset Still's disease (AOSD) patients, with smokers more likely to experience pericarditis, pleuritis, abdominal pain, and complications like macrophage activation syndrome (MAS) and parenchymal lung disease, leading to a poorer prognosis.
CLINICAL RHEUMATOLOGY
(2022)
Article
Rheumatology
Onorina Berardicurti, Viktoriya Pavlych, Ilenia Di Cola, Piero Ruscitti, Paola Di Benedetto, Luca Navarini, Annalisa Marino, Paola Cipriani, Roberto Giacomelli
Summary: This study aimed to assess the long-term safety and efficacy of rituximab (RTX) in primary Sjogren syndrome (pSS) patients with active systemic disease. The results showed that long-term RTX treatment was safe, well tolerated, and effective in reducing disease activity in pSS patients with active systemic disease.
JOURNAL OF RHEUMATOLOGY
(2022)
Article
Rheumatology
Piero Ruscitti, Valentina Natoli, Alessandro Consolaro, Roberta Caorsi, Silvia Rosina, Gabriella Giancane, Roberta Naddei, Ilenia Di Cola, Claudia Di Muzio, Onorina Berardicurti, Daniela Iacono, Ilenia Pantano, Gelsomina Rozza, Silvia Rossi, Ludovico De Stefano, Silvia Balduzzi, Antonio Vitale, Francesco Caso, Luisa Costa, Marcella Prete, Luca Navarini, Annamaria Iagnocco, Fabiola Atzeni, Giuliana Guggino, Federico Perosa, Luca Cantarini, Bruno Frediani, Carlomaurizio Montecucco, Francesco Ciccia, Paola Cipriani, Marco Gattorno, Roberto Giacomelli, Angelo Ravelli
Summary: This study compares the clinical features and treatments of systemic JIA and adult-onset Still's disease. The results reveal remarkable disparities in the prevalence of clinical manifestations between the two illnesses, which may partly depend on their classification by different criteria.
Article
Urology & Nephrology
Angelica Canossi, Samuele Iesari, Quirino Lai, Simone Ciavatta, Tiziana Del Beato, Alessandra Panarese, Barbara Binda, Alessandra Tessitore, Franco Papola, Francesco Pisani
Summary: This study examines the expression patterns of Treg-related genes in peripheral blood before and after kidney transplantation and their association with acute rejection, DSA development, and renal dysfunction. The results suggest that reduced FOXP3 levels and increased solCTLA-4 levels during acute rejection may contribute to graft rejection and renal dysfunction. Additionally, higher pre-transplant solCTLA-4 levels are associated with a lower risk of DSA development.
Article
Rheumatology
Giuseppe Lopalco, Vincenzo Venerito, Antonio Brucato, Giacomo Emmi, Roberto Giacomelli, Alberto Cauli, Matteo Piga, Paola Parronchi, Mariangela Nivuori, Danilo Malandrino, Piero Ruscitti, Gianfranco Vitiello, Claudia Fabiani, Luca Cantarini, Florenzo Iannone
Summary: Anakinra appears to be a safe and effective therapeutic option for patients refractory to traditional anti-inflammatory treatments in polyserositis, showing significant efficacy in reducing recurrences and corticosteroid use.
Article
Rheumatology
Piero Ruscitti, Francesco Ursini, Onorina Berardicurti, Francesco Masedu, Emanuele Bozzalla Cassione, Susanna Naldi, Ilenia Di Cola, Claudia Di Muzio, Ludovico De Stefano, Elena Di Nino, Luca Navarini, Marta Vomero, Serena Bugatti, Marco Valenti, Erminia Mariani, Annamaria Iagnocco, Carlomaurizio Montecucco, Roberto Giacomelli, Paola Cipriani
Summary: The study found that in patients with adult-onset Still's disease complicated by macrophage activation syndrome, levels of cytokines (IL-1, IL-6, IFN-gamma, etc.) and inflammatory markers (ferritin) were elevated. Analysis of circulating immune cells in MAS patients showed an increase in 'classical monocytes' and a reduction in total NK cells. A total of 3477 IFN-related genes were identified in AOSD synovial tissues.
Article
Chemistry, Medicinal
Piero Ruscitti, Vasiliki Liakouli, Noemi Panzera, Adriano Angelucci, Onorina Berardicurti, Elena Di Nino, Luca Navarini, Marta Vomero, Francesco Ursini, Daniele Mauro, Vincenza Dolo, Francesco Ciccia, Roberto Giacomelli, Paola Cipriani
Summary: This study found that the expression of markers of myofibroblast differentiation in the synovial tissue of rheumatoid arthritis patients was significantly higher than in healthy controls, and that TGF-beta and IL-6 stimulation promoted the expression of these markers; tofacitinib may have a role in reducing the expression of these markers.
Article
Biochemistry & Molecular Biology
Luca Navarini, Marta Vomero, Stefano Di Donato, Damiano Currado, Onorina Berardicurti, Annalisa Marino, Pietro Bearzi, Alice Biaggi, Matteo Ferrito, Piero Ruscitti, Marina Fava, Alessandro Leuti, Paola Cipriani, Mauro Maccarrone, Roberto Giacomelli
Summary: This study found that 2-AG modulates the production of type 2 interferon in CD4+ T lymphocytes from SLE patients through CB2 activation.
Article
Immunology
Elvira Favoino, Ettore Grapsi, Giovanna Barbuti, Vasiliki Liakouli, Piero Ruscitti, Caterina Foti, Roberto Giacomelli, Federico Perosa
Summary: This study evaluated whether anti-centromeric protein A (CENP-A) antibodies cross-react with mitochondrial antigens. The results showed that antibodies with a specific mitochondrial antigen (PDC-E2) cross-reacted with anti-centromere antibodies (Ap1-17). This finding is important for investigating the pathogenic significance of these antibodies.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2023)
Review
Medicine, General & Internal
Onorina Berardicurti, Annalisa Marino, Irene Genovali, Luca Navarini, Settimio D'Andrea, Damiano Currado, Amelia Rigon, Luisa Arcarese, Marta Vadacca, Roberto Giacomelli
Summary: Pulmonary involvement is common in patients with pSS, with a comorbid ILD prevalence of at least 20%. NSIP is the most common presentation of pSS-ILD.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Rheumatology
E. Favoino, G. Catacchio, A. Mininni, P. Ruscitti, V. Riccieri, V. Liakouli, A. Corrado, L. Navarini, F. Ciccia, P. Cipriani, F. P. Cantatore, G. Valesini, R. Giacomelli, F. Perosa
Summary: Anti-p4.2 antibodies can identify a subset of systemic sclerosis (SSc) patients at a high risk of developing pulmonary vascular disease (PVD), even in the absence of pulmonary fibrosis (PF).
CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chiara Compagnoni, Veronica Zelli, Andrea Bianchi, Antinisca Di Marco, Roberta Capelli, Davide Vecchiotti, Laura Brandolini, Anna Maria Cimini, Francesca Zazzeroni, Marcello Allegretti, Edoardo Alesse, Alessandra Tessitore
Summary: This study analyzed the regulation of microRNA levels in corneal cells in response to recombinant human NGF (rhNGF) treatment, and identified key genes and pathways involved in neurotrophin signaling and cell proliferation and apoptosis control.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
