Article
Biotechnology & Applied Microbiology
Xinyang Li, Yixin Li, Yiliang Zhou, Jiamin Wu, Zhifeng Zhao, Jiaqi Fan, Fei Deng, Zhaofa Wu, Guihua Xiao, Jing He, Yuanlong Zhang, Guoxun Zhang, Xiaowan Hu, Xingye Chen, Yi Zhang, Hui Qiao, Hao Xie, Yulong Li, Haoqian Wang, Lu Fang, Qionghai Dai
Summary: DeepCAD-RT is a real-time denoising method for fluorescence time-lapse images, which uses self-supervised deep learning for noise suppression. Compared to traditional methods, DeepCAD-RT significantly reduces the number of network parameters, memory consumption, and processing time, and achieves high imaging signal-to-noise ratio with one-tenth of the photons. In various photon-limited experiments, DeepCAD-RT demonstrates its utility in morphological and functional interrogation of biological dynamics.
NATURE BIOTECHNOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Haitao Tang, Sibo Liu, Zhaohui Fang, Ze Yang, Zhenzhen Cui, Hongyu Lv, Peng Zhang, Dawei Wang, Feng Zhao, Jianbei Qiu, Xue Yu, Xuhui Xu
Summary: This study utilizes Ba2LaF7:Tb3+ nanocrystals to achieve high-resolution X-ray imaging and demonstrates excellent optical storage capability. This method can simplify X-ray imaging systems.
ADVANCED OPTICAL MATERIALS
(2022)
Article
Multidisciplinary Sciences
Yasuyuki Kikuchi, Daiyu Ito, Sayaka Wakayama, Masatoshi Ooga, Teruhiko Wakayama
Summary: A new time-lapse observation system for preimplantation embryos was developed in this study, using a simple culture method and a glass capillary. The system allows the observation of mammalian preimplantation embryos without the need for expensive equipment and advanced techniques. The results confirmed that the developmental speed and quality of the embryos were not affected.
SCIENTIFIC REPORTS
(2023)
Article
Geochemistry & Geophysics
Xingye Liu, Xiaohong Chen, Min Bai, Yangkang Chen
Summary: Seismic image registration is crucial for the joint interpretation of multivintage seismic images in time-lapse reservoir monitoring. The method of high-resolution time-shift analysis spectrum can help with manual and automatic picking, improving registration accuracy.
Article
Geochemistry & Geophysics
Shan Qu, Dirk Jacob Verschuur
Summary: High-resolution simultaneous joint migration inversion is an effective time-lapse tool that combines data processing strategy and joint migration inversion method to dynamically suppress non-repeatable uncertainties during time-lapse processing.
GEOPHYSICAL PROSPECTING
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Han Gyu Kang, Hideaki Tashima, Hidekatsu Wakizaka, Fumihiko Nishikido, Makoto Higuchi, Miwako Takahashi, Taiga Yamaya
Summary: The ultrahigh-resolution small-animal PET scanner we developed can provide a resolution approaching 0.6 mm, allowing for unprecedented visualization of mouse brain function. It consists of 4 rings with 16 depth-of-interaction detectors each, and has shown promising results for neuroscience research using rodent models.
JOURNAL OF NUCLEAR MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Joanna Kochan, Agnieszka Nowak, Barbara Kij, Sylwia Prochowska, Wojciech Nizanski
Summary: The study identified that the timing of the first and second cleavage divisions, the timing of blastocyst cavity formation, and morphological anomalies are early and non-invasive indicators of cat embryo development in vitro.
Article
Obstetrics & Gynecology
Amanda Setti, Daniela Braga, Patricia Guilherme, Assumpto Iaconelli, Edson Borges
Summary: This study found that increasing oocyte immaturity rate is associated with delayed cell cleavage and blastulation. These findings highlight the importance of TLI for identifying and deselecting slow-growing embryos for transfer in cycles with high oocyte immaturity rate.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Review
Medicine, General & Internal
JinLuan Wang, Ying Guo, Ning Zhang, TingTing Li
Summary: Cultivation and selection of high-quality human embryos are critical for the success of in vitro fertilization-embryo transfer. Time-lapse imaging technology (TLI) provides a stable culture environment for embryos and allows accurate recording of embryo development time parameters. Studies have shown the effectiveness of TLI in assessing embryo development potential, but further research is needed to confirm and explore the predictive significance of various embryo kinetic parameters.
Article
Biology
Guanping Feng, Aby Joseph, Kosha Dholakia, Fei Shang, Charles W. Pfeifer, Derek Power, Krishnan Padmanabhan, Jesse Schallek
Summary: In recent years, it has been possible to image single neurons, glia, and vascular cells in vivo to study local networks of activity in the living brain. However, a similar method has not yet been developed for the constantly moving eye. In this study, a novel head-fixed preparation was characterized to enable high-resolution retinal imaging at the single-cell level in awake mice. The study revealed new functional attributes of the eye that are overlooked by anesthesia and highlighted the benefits of studying retinal physiology without anesthesia.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Shabana Sayed, Marte Myhre Reigstad, Bjorn Molt Petersen, Arne Schwennicke, Jon Wegner Hausken, Ritsa Storeng
Summary: The primary purpose of this study was to investigate the association between nucleation status of preimplantation embryos on Day 2 and live births following IVF. The analysis was based on 2769 transferred embryos and revealed that the frequency of nucleation errors was 23.8%. Embryos with nucleation errors at the two-cell stage had significantly lower live birth rates. Furthermore, increased age, delayed cell divisions, and insemination with surgically retrieved spermatozoa were identified as factors associated with higher incidences of nucleation errors.
Article
Biochemical Research Methods
Romain Fernandez, Cedric Moisy
Summary: The increasing interest in biomedical 3D imaging devices from animal and plant research communities has led to new research topics. Combining outputs from imaging devices such as X-ray CT and MRI scans allows non-destructive observation of tissue anatomy, structure, and function in time-lapse multimodal imaging experiments.
Living samples cannot remain in these devices for long periods, leading to variations in shape, position, and orientation in the acquired images. A preprocessing step of 3D registration is required to address these variations before analysis. Fijiyama, a Fiji plugin built upon biomedical registration algorithms, aims to facilitate automatic alignment of 3D images for non-specialists.
Article
Obstetrics & Gynecology
Jiahong Zhu, Lihong Wu, Junxia Liu, Yanfeng Liang, Jiayi Zou, Xiangwei Hao, Guoning Huang, Wei Han
Summary: iDAScore Version 2.0 performed as well as, or better than, conventional morphological assessment in fresh day 3 embryo transfer cycles. iDAScore Version 2.0 may therefore constitute a promising tool for selecting embryos with the highest likelihood of implantation.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Biochemistry & Molecular Biology
Tongchao Li, Tian-Ming Fu, Kenneth Kin Lam Wong, Hongjie Li, Qijing Xie, David J. Luginbuhl, Mark J. Wagner, Eric Betzig, Liqun Luo
Summary: The study investigates the dynamic cellular processes by which olfactory receptor neurons target axons to specific glomeruli in the olfactory circuit of Drosophila. Through time-lapse imaging and fast imaging techniques, the researchers identified the mechanisms involved in ipsilateral targeting and revealed the dynamics of axon branching upon approaching the target. These observations provide insights into the cellular basis of wiring specificity establishment.
Article
Reproductive Biology
E. S. van Marion, J. P. Speksnijder, J. Hoek, W. P. A. Boellaard, M. Dinkelman-Smit, E. A. Chavli, R. P. M. Steegers-Theunissen, J. S. E. Laven, E. B. Baart
Summary: Human embryos derived from fertilization with testicular sperm exhibit a prolonged pronuclear stage and more frequent direct unequal cleavage compared to embryos fertilized with ejaculated sperm, while subsequent cleavage divisions are not affected.
BIOLOGY OF REPRODUCTION
(2021)
Article
Chemistry, Analytical
Jung-Cheng Su, Yi-Ju Liu, Da-Jeng Yao
Summary: This article introduces the application of an electrowetting-on-dielectric (EWOD) chip in the field of biomedicine, which can accurately control volume and reduce the amount of samples and reagents used. By using the ImageJ image processing tool, optimal operating parameters were determined, and successful biochemical identification of five bacterial species was achieved. The experimental results show that the DBEDF chip has high operational efficiency and can be applied in microbial identification and other fields.
Article
Engineering, Chemical
Parkarsh Kumar, Da-Jeng Yao
Summary: Experiments were conducted to study the impact of temperature and humidity on the process of vacuum freeze-drying. The findings led to the development of an efficient and optimized drying process. The start of primary and secondary drying was found to be influenced by relative humidity.
JOURNAL OF FOOD PROCESS ENGINEERING
(2022)
Review
Biochemistry & Molecular Biology
Anand Baby Alias, Shubhanvit Mishra, Gaurav Pendharkar, Chi-Shuo Chen, Cheng-Hsien Liu, Yi-Ju Liu, Da-Jeng Yao
Summary: Microalgae are a significant source of renewable, sustainable and economical biofuels, with vast potential applications in energy, pharmaceutical and nutraceutical industries. The utilization of microfluidic technology plays a vital role in microalgal processes and applications in biofuels and biosensing.
Article
Chemistry, Analytical
Anand Baby Alias, Hong-Yuan Huang, Yi-Wen Wang, Kai-Ti Lin, Pei-Jhen Lu, Tzu-Hui Wu, Pei-Shin Jiang, Chien-An Chen, Da-Jeng Yao
Summary: This study utilizes Electro-Wetting-On-Dielectric (EWOD) based digital operations to extract subcritical concentration of cell-free DNA (cf-DNA), and amplifies it to the nanogram range for further analysis. The natural growth of the embryo is not disrupted during the extraction process, paving a new path for the lab-on-a-chip (LOC) concept.
Article
Chemistry, Multidisciplinary
Bo-Wei Chen, Shadie Hatamie, Hsin-Cheng Chiu, Zung-Hang Wei, Shang-Hsiu Hu, Da-Jeng Yao
Summary: This study develops core-shell cubes that enhance the coercivity and magnetization of magnetic nanoparticles (MNPs) through carefully tuning the interface relaxation. These cubes maximize the hyperthermia efficacy under an alternating magnetic field (AMF) and can target cancer cells and penetrate into tumors, potentially being used for clinical applications.
ADVANCED MATERIALS INTERFACES
(2022)
Article
Biochemical Research Methods
Ting-Yao Yen, Da-Jeng Yao
Summary: An electronic-nose system based on surface acoustic wave (SAW) was used to measure the freshness of kiwifruit by analyzing the frequency shift caused by different polymers. The research proved that discrimination is feasible from an unripe stage to a ripe stage and from a ripe stage to an over-ripe stage.
IEEE TRANSACTIONS ON NANOBIOSCIENCE
(2022)
Article
Chemistry, Analytical
Koki Yamamoto, Kyojiro Morikawa, Chihchen Chen, Takehiko Kitamori
Summary: The lifetime of enzymes plays a critical role in preventing system failure and optimizing maintenance schedules in biological and analytical chemistry. Enzyme activity can be used to evaluate the lifetime metrics, which are measured in terms of catalytic cycles per enzyme at different storage times. This study focuses on investigating the catalytic cycles of trypsin in a nanochannel and evaluating the optimal immobilization process. Results showed that the immobilized enzyme densities and activities remained consistent after 72 hours of enzyme storage, indicating successful suppression of self-digestion. The calculated catalytic cycles were 1740, and the reaction efficiency was evaluated based on these cycles for the first time while preventing self-digestion by trypsin.
ANALYTICAL SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Pooyan Heravi, Li-An Chu, Da-Jeng Yao
Summary: Lateral flow is crucial in microfluidic applications, but there is a lack of design rules or mathematical models to predict its characteristics. This study provides an empirical dimensionless model for lateral flow induced by fluid inhomogeneity and validates it through experiments. The model accurately predicts the rotation caused by lateral flow and is applicable to various types of fluid inhomogeneity. The results can be used to improve the design of microfluidic devices dealing with lateral flow, density disparity, mixing, and chemical reaction.
MICROFLUIDICS AND NANOFLUIDICS
(2023)
Article
Nanoscience & Nanotechnology
Pooyan Heravi, Li-An Chu, Da-Jeng Yao
Summary: Horizontally stratified flows are commonly observed in micro-scale engineering problems. Recent studies have shown that diffusion at the liquid interface can lead to a lateral flow, which has the potential to disrupt or be utilized in device designs. This investigation examines the effects of different inlet junctions on the flow field in microfluidic devices using numerical and experimental methods. The results reveal that the counter-flow T junction produces the least lateral flow, while the straight channel produces the highest. Understanding these differences can improve the design of microfluidic devices for various applications.
Article
Thermodynamics
Pooyan Heravi, Li-An Chu, Da-Jeng Yao
Summary: Horizontally stratified flows in microfluidics offer unique opportunities but also introduce complexity into these systems. This study shows that the quasi-interface in these flows can rotate due to diffusion, density changes, and gravitational forces, even with homogeneous density and non-dominant transversal diffusion. Ignoring any link in this phenomenon chain will break the chain of events and fail to predict the combined effect. Understanding and mitigating this rotation phenomenon is crucial for designing efficient microfluidic devices with miscible liquid interfaces.
EXPERIMENTAL THERMAL AND FLUID SCIENCE
(2023)
Article
Engineering, Electrical & Electronic
Kyojiro Morikawa, Po-yin Chen, Hai Linh Tran, Yutaka Kazoe, Chihchen Chen, Takehiko Kitamori
Summary: Channel fabrication technology is essential for microfluidic and nanofluidic devices. Glass channels are attractive due to their stability, transparency, and ease of modification. This study developed a microchannel fabrication method with high etching selectivity and smooth surface.
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2023)
Article
Chemistry, Analytical
Yi-Wei Lin, Chang-Yi Chen, Ying-Fang Chang, Yii-Nuoh Chang, Da-Jeng Yao
Summary: UV-curable resin was formed into different patterns through the programmable control of dielectric force. The control factors affecting the size of the UV resin driving area were investigated, and it was found that current played an important role. By changing the current and frequency, a more accurate shape and fine control of the pattern line width could be achieved.
Article
Chemistry, Physical
Duane S. Juang, Yi-Ren Huo, Hsin-Kai Tseng, Chih-Chen Chen, Chia-Hsien Hsu, Chun-Cheng Lin
Summary: Enzymes are important catalysts in biochemical reactions, but face drawbacks such as product inhibition and difficulty recycling. This study presents a microfluidic reactor that removes products and adds fresh substrate in a countercurrent design, improving enzyme reaction conditions. The countercurrent flow was shown to accelerate enzymatic reactions by 36% for horseradish peroxidase and 21% for β-glucosidase, producing highly pure products with minimal substrate contamination.
Article
Chemistry, Analytical
Lin-Yen Yu, Chia-Hsien Hsu, Chia-Yang Li, Shiao-Ya Hong, Chaang-Ray Chen, Chi-Shuo Chen
Summary: The study developed a high-throughput 3D tumor spheroid platform to evaluate the efficacy of boron neutron capture therapy (BNCT) for pancreatic cancer treatment. The results showed that hypoxia and fibrosis can reduce the effectiveness of BNCT in treating pancreatic cancer.