Article
Biology
Shilpa Bhandi, Ahmed Alkahtani, Mohammed Mashyakhy, Hosam Ali Baeshen, Mohammed Mustafa, Hitesh Chohan, Nezar Boreak, Shankargouda Patil
Summary: This study demonstrated that culturing DPSCs in media with different combinations of serum and glucose affects their proliferation and osteogenic potential. High glucose and high serum conditions inhibit the growth and differentiation of DPSCs, indicating a need to reconsider culture conditions for optimal utilization of their potential.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Cell Biology
Francesca Santilli, Jessica Fabrizi, Stefano Martellucci, Costantino Santacroce, Egidio Iorio, Maria Elena Pisanu, Mattea Chirico, Loreto Lancia, Fanny Pulcini, Valeria Manganelli, Maurizio Sorice, Simona Delle Monache, Vincenzo Mattei
Summary: This study analyzed the lipid composition of human Dental Pulp-Derived Stem Cells (DPSCs) and investigated the role of lipid rafts during multilineage differentiation. The results showed that mono-unsaturated fatty acids were the most representative species in the acyl chains of DPSCs. Moreover, disrupting lipid rafts prevented AKT activation and lineage-specific mRNA expression during multilineage differentiation, indicating the importance of lipid raft integrity in DPSCs differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Giulia Brunello, Federica Zanotti, Martina Trentini, Ilaria Zanolla, Elham Pishavar, Vittorio Favero, Riccardo Favero, Lorenzo Favero, Eriberto Bressan, Massimo Bonora, Stefano Sivolella, Barbara Zavan
Summary: This study found that EXOs derived from dental pulp stem cells of different age donors have varying effects on MSCs. The younger donor group showed a more significant influence on proliferation and commitment ability.
Article
Biochemistry & Molecular Biology
Anastasia Kotova, Arseniy A. Lobov, Julia A. Dombrovskaya, Valentina Y. Sannikova, Nadezhda A. Ryumina, Polina Klausen, Alexey L. Shavarda, Anna B. Malashicheva, Natella Enukashvily
Summary: Dental stem cells, derived from neural crest stem cells, exhibit differences in functional and biological properties, including neural markers, cell morphology, proliferation rate, and osteogenic differentiation capabilities. Dental pulp stem cells and periodontal ligament stem cells show distinctions in neural markers, nuclear protein expression, proliferation rate, etc.
Article
Biochemistry & Molecular Biology
Wushuang Huang, Qi Huang, Hongwen He, Fang Huang
Summary: This study aimed to investigate the effect and potential mechanism of PER2 on the differentiation of human dental pulp stem cells (hDPSCs). The results showed that PER2 promoted the odontoblastic/osteogenic differentiation of hDPSCs, and this process involved mitochondrial metabolic changes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Article
Biotechnology & Applied Microbiology
Nunthawan Nowwarote, Stephane Petit, Francois Come Ferre, Florent Dingli, Victor Laigle, Damarys Loew, Thanaphum Osathanon, Benjamin P. J. Fournier
Summary: The study identified the matrisome of ECM proteins derived from human DPSCs and found that it can regulate mineralization, even in cells which do not respond to mineralization assaults like GF.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Keiji Masuda, Xu Han, Hiroki Kato, Hiroshi Sato, Yu Zhang, Xiao Sun, Yuta Hirofuji, Haruyoshi Yamaza, Aya Yamada, Satoshi Fukumoto
Summary: Dental pulp stem cells, derived from multipotent neural crest cells, have high proliferative capacity and multipotent potential for various applications. Stem cells donated by patients with genetic disorders can serve as disease models. Ethical considerations and potential applications of these stem cells donated and reused are worth exploring.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Kangrui Zeng, Weiping Li, Qiongyi Kang, Yutong Li, Qing Cheng, Wenwei Xia
Summary: This study revealed that miR-342-5p inhibits the odonto/osteogenic differentiation of hDPSCs by interfering with Wnt/beta-catenin signaling via targeting Wnt7b.
Article
Biochemistry & Molecular Biology
Martyna Smeda, Kerstin M. Galler, Melanie Woelflick, Andreas Rosendahl, Christoph Moehle, Beate Lenhardt, Wolfgang Buchalla, Matthias Widbiller
Summary: Both dental pulp and apical papilla are potential sources of stem cells for regenerative endodontic treatments. This study compared the molecular characteristics of dental pulp stem cells (DPSC) and stem cells from the apical papilla (SCAP). The results showed that both cell types expressed similar mesenchymal stem cell markers and had the ability to differentiate into various lineages. However, differences were observed in the profiles of secreted molecules and gene regulation. Overall, both DPSC and SCAP are suitable for dental pulp tissue engineering from a molecular biological perspective.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
J. F. Huo, M. L. Zhang, X. X. Wang, D. H. Zou
Summary: The study found that chrysin can promote osteogenic differentiation of DPSCs and enhance DPSC-based bone formation in nude mice and rat models. Proteomics analysis revealed the crucial role of Smad3 in this process.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Charlton Bassett, Hunter Triplett, Keegan Lott, Katherine M. Howard, Karl Kingsley
Summary: This study evaluated the expression of specific microRNAs involved in neuronal differentiation in dental pulp stem cells (DPSCs) and found differential expression of miR-27 and miR-145. The study also demonstrated that treatment with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) can modulate the expression of miR-27 and miR-145 in responsive DPSCs, suggesting the need for further investigation into the downstream targets of these microRNAs.
Article
Biochemistry & Molecular Biology
Chatvadee Kornsuthisopon, Sunisa Rochanavibhata, Nunthawan Nowwarote, Kevin A. Tompkins, Waleerat Sukarawan, Thanaphum Osathanon
Summary: The small molecule 6-bromoindirubin-3'-oxime (BIO) effectively modulates the osteogenic differentiation of hDPSCs by activating the Wnt signaling pathway. BIO promotes alkaline phosphatase activity and mineralization, while inhibiting colony formation, cell migration, and adipogenic differentiation. These findings suggest that BIO has potential as a bioactive molecule to enhance dentin repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Francesco Paduano, Elisabetta Aiello, Paul Roy Cooper, Benedetta Marrelli, Irina Makeeva, Mohammad Islam, Gianrico Spagnuolo, Davide Maged, Danila De Vito, Marco Tatullo
Summary: Dental stem cells have the potential to differentiate into various cell types and can revert back to a stem-like state after osteogenic differentiation, showing a greater potential for further differentiation. The ability to control dedifferentiation of DSCs under physiological conditions could enhance their therapeutic potential in bone tissue engineering.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Environmental
Wenting Yu, Xiaoxia Su, Moxiao Li, Wanting Wan, Ang Li, Hong Zhou, Feng Xu
Summary: This study investigated the effects of matrix strain on the characteristics and bioactivity of MSC-derived exosomes by culturing PDLSCs in three-dimensional magnetically stretched collagen hydrogels. The results showed that altering the miRNA content in exosomes significantly enhanced their bioactivity, leading to improved repair of bone defects in vivo. This research suggests a novel strategy for promoting tissue regeneration through engineered mechanical microenvironments.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Dentistry, Oral Surgery & Medicine
Hiroki Murano, Tomoatsu Kaneko, Su Yee Myo Zaw, Phyo Pyai Sone, Zar Chi Thein Zaw, Yamato Okada, Mitsuhiro Sunakawa, Ken-ichi Katsube, Takashi Okiji
Summary: The application of mustard oil to induce pulpal inflammation resulted in transient down-regulation of the Kcna1 gene, transient reduction in the density of Kv1.1-expressing axons, and increased astrocytes in the thalamus within 1 hour, suggesting central sensitization represented by neuronal hyperexcitability and astrocyte activation.
Article
Dentistry, Oral Surgery & Medicine
Htoo Shwe Sin Thein, Kentaro Hashimoto, Nobuyuki Kawashima, Sonoko Noda, Takashi Okiji
Summary: S-PRG sealer extracts have suppressive effects on proinflammatory cytokine expression, possibly involving Zn2+ ions.
DENTAL MATERIALS JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
Sherif Adel, Kentaro Hashimoto, Nobuyuki Kawashima, Takahiro Wada, Motohiro Uo, Takashi Okiji
Summary: The study demonstrates that S(3)A exhibits biocompatibility and pro-mineralization effects comparable to MTA. It promotes mineralized tissue repair and shows higher mineralized nodule formation and gene expression related to bone formation compared to MTA.
JOURNAL OF DENTAL SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Zar Chi Thein Zaw, Nobuyuki Kawashima, Tomoatsu Kaneko, Takashi Okiji
Summary: This study demonstrates neovascularization during coronal pulp regeneration in rat molars and suggests that rDPC-endothelial cell interactions can enhance angiogenic capacity in vitro.
JOURNAL OF DENTAL SCIENCES
(2022)
Article
Chemistry, Physical
Kunlanun Dumrongvute, Sherif Adel, Takahiro Wada, Nobuyuki Kawashima, Chinalai Piyachon, Hiroshi Watanabe, Tohru Kurabayashi, Takashi Okiji, Motohiro Uo
Summary: This study synthesized distrontium cerate (2SrO·CeO2: S2Ce) and evaluated its properties as an alternative component of endodontic cement. The S2Ce cement demonstrated a rapid setting time and high compressive strength. It released strontium ions and exhibited high radiopacity. These findings suggest that S2Ce could be a suitable cement material for endodontic applications.
Article
Multidisciplinary Sciences
Shion Orikasa, Nobuyuki Kawashima, Kento Tazawa, Kentaro Hashimoto, Keisuke Sunada-Nara, Sonoko Noda, Mayuko Fujii, Tetsu Akiyama, Takashi Okiji
Summary: This study revealed that BCL9 plays a crucial role in the osteo/odontoblastic differentiation of dental pulp stem cells (DPSCs) under hypoxic conditions. This differentiation process is partially dependent on the Wnt/β-catenin signaling pathway, with BCL9 acting as a key mediator between HIF1 alpha and Wnt/β-catenin signaling.
SCIENTIFIC REPORTS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Moe S. Kyaw, Arata Ebihara, Keiichiro Maki, Shunsuke Kimura, Taro Nakatsukasa, Pyae H. Htun, Myint Thu, Satoshi Omori, Takashi Okiji
Summary: Under laboratory conditions, the optimal glide path motion generated less clockwise torque and greater upward force compared to other rotation motions. The optimal glide path motion also resulted in fewer surface defects compared to continuous rotation. The three modes of reciprocal rotation better maintained the apical curvature of root canals than continuous rotation with the ProGlider instrument.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
Aseel Alchawoosh, Kentaro Hashimoto, Nobuyuki Kawashima, Sonoko Noda, Kosuke Nozaki, Takashi Okiji
Summary: This study evaluated the effects of HCSB sealers BioC, WST, and BC on osteoblastic differentiation/mineralization and proinflammatory cytokine synthesis. The results showed that these three sealers promoted osteoblastic differentiation/mineralization and downregulated the expression of proinflammatory cytokines.
JOURNAL OF DENTAL SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Myint Thu, Arata Ebihara, Keiichiro Maki, Miki Nishijo, Shunsuke Kimura, Taro Nakatsukasa, Moe Sandar Kyaw, Takashi Okiji
Summary: This study evaluated the effect of different metallurgy and faster upward speed pecking motions on the dynamic cyclic fatigue resistance of nickel-titanium rotary instruments. The results showed that the fastest upward speed group exhibited higher cyclic fatigue resistance, and ProTaper Gold instruments performed better in terms of fatigue resistance in all groups.
Article
Biochemistry & Molecular Biology
Peifeng Han, Keisuke Sunada-Nara, Nobuyuki Kawashima, Mayuko Fujii, Shihan Wang, Thoai Quoc Kieu, Ziniu Yu, Takashi Okiji
Summary: This study found that miR-146b-5p can suppress inflammation in human dental pulp cells stimulated by LPS, and its synthesis is controlled via the NF-κB/IL6/STAT3 signaling cascade. The study also found that miR-146b-5p down-regulates the expression of pro-inflammatory mediators by targeting TRAF6, IRAK1, and RELA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Myint Thu, Arata Ebihara, Keiichiro Maki, Shunsuke Kimura, Moe-Sandar Kyaw, Yuka Kasuga, Miki Nishijo, Takashi Okiji
Summary: In single-curved canals, TN yielded significantly higher torque values compared to RE, whereas in double-curved canals it was higher than PG. In single-curved canals, EDM exhibited significantly higher screw-in force compared to TN and RE, while in double-curved canals there was no significant difference.
JOURNAL OF DENTAL SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Naoto Ohkura, Kunihiko Yoshiba, Nagako Yoshiba, Yohei Oda, Naoki Edanami, Hayato Ohshima, Shoji Takenaka, Takashi Okiji, Yuichiro Noiri
Summary: This study investigated the localization of components of the PGE2-transporting pathway in human dental pulp and their relevance to angiogenesis and dentinogenesis. The results showed that EP2/EP4 agonists promoted the generation of endothelial cell filopodia and upregulated the expression of angiogenesis and odontoblast differentiation-related genes.
JOURNAL OF ENDODONTICS
(2023)
Article
Cell & Tissue Engineering
Yoshio Yahata, Keisuke Handa, Naoto Ohkura, Motoki Okamoto, Jun Ohshima, Shusaku Itoh, Nobuyuki Kawashima, Toshinori Tanaka, Nobuya Sato, Yuichiro Noiri, Mikako Hayashi, Takashi Okiji, Masahiro Saito
Summary: This randomized multicenter trial evaluated the effects of concentrated growth factor (CGF) on bone healing in combination with root-end microsurgery. The results showed that CGF in conjunction with root-end microsurgery accelerated lesion reduction, indicating it as a promising treatment option.
REGENERATIVE THERAPY
(2023)