Pichia pastoris protease‐deficient and auxotrophic strains generated by a novel, user‐friendly vector toolbox for gene deletion

Extraction of genomic DNA from yeasts for PCR-based applications

(2018) Marko Lõoke et al.   BIOTECHNIQUES

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

(2017) Astrid Weninger et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Creation of Stable Heterothallic Strains of Komagataella phaffii Enables Dissection of Mating Gene Regulation

(2017) Lina Heistinger et al.   MOLECULAR AND CELLULAR BIOLOGY

A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants in Pichia pastoris

(2017) Ding Li et al.   Frontiers in Microbiology

Refined Pichia pastoris reference genome sequence

(2016) Lukas Sturmberger et al.   JOURNAL OF BIOTECHNOLOGY

Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing

(2016) Jan-Philipp Schwarzhans et al.   Scientific Reports

Improved Gene Targeting through Cell Cycle Synchronization

(2015) Vasiliki Tsakraklides et al.   PLoS One

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Disruption of YPS1 and PEP4 genes reduces proteolytic degradation of secreted HSA/PTH in Pichia pastoris GS115

(2013) Min Wu et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Enhancement of the Gene Targeting Efficiency of Non-Conventional Yeasts by Increasing Genetic Redundancy

(2013) Zao Chen et al.   PLoS One

Identification and deletion of the major secreted protein of Pichia pastoris

(2012) Silvia Heiss et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Absence of Yps7p, a putative glycosylphosphatidylinositol-linked aspartyl protease inPichia pastoris, results in aberrant cell wall composition and increased osmotic stress resistance

(2012) Bo Guan et al.   FEMS YEAST RESEARCH

Homologous recombination and its regulation

(2012) L. Krejci et al.   NUCLEIC ACIDS RESEARCH

Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

(2012) Laura Näätsaari et al.   PLoS One

Sequential deletion of Pichia pastoris genes by a self-excisable cassette

(2011) Rongqing Pan et al.   FEMS YEAST RESEARCH

High-quality genome sequence of Pichia pastoris CBS7435

(2011) Andreas Küberl et al.   JOURNAL OF BIOTECHNOLOGY

SILAC Compatible Strain ofPichia pastorisfor Expression of Isotopically Labeled Protein Standards and Quantitative Proteomics

(2011) Ryan J. Austin et al.   JOURNAL OF PROTEOME RESEARCH

Secretion of an Endogenous Subtilisin by Pichia pastoris Strains GS115 and KM71

(2010) K. Salamin et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

mazFas a counter-selectable marker for unmarked genetic modification ofPichia pastoris

(2009) Junjie Yang et al.   FEMS YEAST RESEARCH

Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris

(2009) Diethard Mattanovich et al.   Microbial Cell Factories

Genome sequence of the recombinant protein production host Pichia pastoris

(2009) Kristof De Schutter et al.   NATURE BIOTECHNOLOGY

Direct selection ofPichia pastoris expression strains using new G418 resistance vectors

(2008) Joan Lin-Cereghino et al.   YEAST