Journal
VIRCHOWS ARCHIV
Volume 475, Issue 2, Pages 175-180Publisher
SPRINGER
DOI: 10.1007/s00428-019-02605-w
Keywords
Latent membrane protein-1 (LMP1); Epstein-Barr virus (EBV); Classic Hodgkin lymphoma (cHL); Follicular dendritic cells (FDCs); Programmed death ligand 1 (PD-L1); Exosomes and microvesicles
Categories
Funding
- Istituto Pasteur Italia Fondazione Cenci Bolognetti
- grant Progetto di Cooperazione Internazionale Didattica e Scientifica con i paesi in via di sviluppo from Sapienza University
Ask authors/readers for more resources
Expression of the latent membrane protein-1 (LMP1) of Epstein-Barr virus (EBV) was investigated in 153 cases of EBV+classic Hodgkin lymphoma (cHL); 120 cases were pediatric patients (< 14 years of age) from Iraq, and 33 cases were adult patients from Italy. We describe for the first time the presence of LMP1 protein in EBV-encoded RNA(EBER)-negative follicular dendritic cells (FDCs) of reactive germinal centers (GC) associated with EBV+cHL. Presence of LMP1+GCs was independent of geographic region and age of patients. Variable numbers of reactive GCs were present in 22.2% of cases (34 of 153), whereas LMP1 staining of FDCs was present in about a third of cases (10 of 34) with reactive GC. Most cases with LMP1+GCweremixed-cellularity (MC) subtype, but some nodular sclerosis (NS) was also present. GC cells with LMP1+FDCs were surrounded by numerous EBV-infected cells which were positive for EBER, LMP1, and CD30. Double immunolocalization analysis revealed that LMP1 was associated with CD63, an exosomal marker, and with CD21. The possibility is discussed that peri-follicular EBV-infected cells release LMP1 protein, perhaps through exosomes, and that the protein is then captured by FDCs and is presented to EBER-negative GC B cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available