Article
Environmental Sciences
Iteb Boughattas, Nesrine Zitouni, Marouane Mkhinini, Omayma Missawi, Sondes Helaoui, Sabrine Hattab, Moncef Mokni, Noureddine Bousserrhine, Mohamed Banni
Summary: This study investigated the effects of biochar on earthworms in soil remediation. The results showed that in the presence of biochar, the bioavailability of Cd and 2,4-D increased in earthworms, along with an increase in micronuclei frequency and a decrease in lysosomal membrane stability. Histopathological examination revealed numerous alterations in animals exposed to contaminants without any amelioration when biochar was added. The biochemical response of earthworms demonstrated that in the presence of biochar, animals tended to alleviate the toxicity of Cd and 2,4-D.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Engineering, Environmental
Maria Rachele Guascito, Maria Giulia Lionetto, Franco Mazzotta, Marianna Conte, Maria Elena Giordano, Roberto Caricato, Anna Rita De Bartolomeo, Adelaide Dinoi, Daniela Cesari, Eva Merico, Laura Mazzotta, Daniele Contini
Summary: In this study, the toxicity of atmospheric particulate matter (PM) was investigated through in vitro experiments. The results showed correlations between extracellular and intracellular toxicity indicators, and significant effects of combustion sources on these toxicity indicators. Additionally, the advection of African dust resulted in lower intrinsic toxicity indicators.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Environmental Sciences
Sabrine Hattab, Iteb Boughattas, Tiziana Cappello, Nesrine Zitouni, Ghada Touil, Ilef Romdhani, Alexandre Livet, Nourreddine Bousserrhine, Mohamed Banni
Summary: Heavy metal pollution has harmful consequences on soil fertility, and earthworms are used as test organisms to evaluate the ecotoxicity of polluted soils. This study exposed Eisenia andrei to polymetallic contaminated soils for 7 and 14 days in southeastern Tunisia. The results showed increased heavy metal accumulation, genotoxicity, and cytotoxicity in earthworms after 14 days of exposure, as well as changes in biochemical and transcriptional responses. These findings support the use of earthworms as bioindicator species for pollution monitoring and provide valuable information about the harmful impact of biota exposure to contaminated soils.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Environmental Sciences
Ingra Tais Malacarne, Daniel Vitor De Souza, Barbara Dos Anjos Rosario, Milena De Barros Viana, Camilo Dias Seabra Pereira, Debora Estadella, Jean Nunes dos Santos, Daniel Araki Ribeiro
Summary: Crack-cocaine can induce genotoxicity and oxidative stress in mammalian cells, and studies have shown evidence supporting its role as a chemical carcinogen. However, the impact of inflammatory response after exposure to crack-cocaine remains inconclusive.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Toxicology
Nancy B. Andrioli, Gabriela Chaufan
Summary: The study evaluated the genotoxicity and oxidative stress in the A549 cell line exposed to sublethal concentrations of Iprodione. The increase in Iprodione concentrations slightly raised the probability of belonging to the genotoxic category, while oxidative stress analysis showed a significant linear increase in SOD and a significant linear decrease in GSH and GST.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biophysics
Masoud Eskandani, Morteza Eskandani, Somayeh Vandghanooni, Bahman Navidshad, Farzad Mirzaei Aghjehgheshlagh, Ali Nobakht
Summary: This study successfully enhanced the bioavailability of L-carnitine (LC) using solid lipid nanoparticles (SLNs) as carriers and demonstrated the protective effects of LC-SLNs against intracellular oxidative stress damages.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Narayanam Phani Satyanarayana Acharyulu, Arya Sohan, Pravallika Banoth, Srinivasu Chintalapati, Sejal Doshi, Venu Reddy, Chella Santhosh, Andrews Nirmala Grace, Luis De Los Santos Valladares, Pratap Kollu
Summary: This study prepared nickel ferrite nanoparticles and nickel/nickel ferrite graphene oxide nanocomposites and evaluated their toxic effects on E. coli cells. The results showed that the nanocomposites induced cell death in E. coli through reactive oxygen species (ROS) generation and oxidative stress.
Review
Biochemistry & Molecular Biology
Monika Sharma, Nikhil Maheshwari, Fahim Halim Khan, Riaz Mahmood
Summary: The extensive use of harmful pesticides in agriculture, including Carbendazim (CBZ), poses serious threats to the environment and human health. CBZ has been found to cause various health problems, including endocrine disruption, embryo toxicity, and neurodegenerative disorders.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Article
Multidisciplinary Sciences
Natalia Janik-Olchawa, Agnieszka Drozdz, Damian Ryszawy, Maciej Pudelek, Karolina Planeta, Zuzanna Setkowicz, Maciej Sniegocki, Magdalena Wytrwal-Sarna, Marta Gajewska, Joanna Chwiej
Summary: This study compared the impact of PEG-coated magnetite nanoparticles with different core sizes on six selected cell lines. It was found that exposure to these nanoparticles led to decreased cell viability, which was dependent on exposure time rather than core diameter. The nanoparticles not only increased the proliferation rate of macrophages but also stimulated tumor cell divisions. Additionally, the smallest nanoparticles mainly induced an increase in ROS levels in cells.
SCIENTIFIC REPORTS
(2021)
Article
Toxicology
Maja Beus, Igor M. Pongrac, Ivona Capjak, Krunoslav Ilic, Ena Vrcek, Marija Curlin, Mirta Milic, Ana Marija Marjanovic Cermak, Ivan Pavicic
Summary: Different surface coatings of silver nanoparticles (AgNPs) have varying toxicity and internalization effects in porcine kidney (PK15) cells. AgNPs with positive surface charge show higher internalization. This study highlights the importance of biosafety evaluation in developing AgNPs-containing materials.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Pharmacology & Pharmacy
Mustafa Gokce, Eray Metin Guler
Summary: This study investigates the toxic effects of A. hierchuntica extracts on melanoma cells, showing significant apoptotic and genotoxic activity in selected B16F10 cancer cell lines. AHE exhibits selectivity towards melanoma cells at specific concentrations, which could potentially be used as an active ingredient in future melanoma treatment protocols.
Article
Oncology
Habib Ghaznavi, Mohammad Reza Hajinezhad, Milad Shirvaliloo, Sheida Shahraki, Kourosh Shahraki, Ramin Saravani, Sakine Shirvalilou, Omolbanin Shahraki, Ziba Nazarlou, Roghayeh Sheervalilou, Saman Sargazi
Summary: The aim of this study was to evaluate the cytotoxicity, genotoxicity, and histopathological effects of Fe2O3@Au-FA NPs using in vitro and in vivo models. The results showed no significant toxicity of the nanoparticles at the safe dose in both in vitro and in vivo experiments. These findings suggest that Fe2O3@Au-FA NPs could be potential candidates in the field of nanobiomedicine at appropriate doses.
Article
Environmental Sciences
Maria Giulia Lionetto, Maria Rachele Guascito, Maria Elena Giordano, Roberto Caricato, Anna Rita De Bartolomeo, Maria Pia Romano, Marianna Conte, Adelaide Dinoi, Daniele Contini
Summary: This study investigated the effects of atmospheric particulate matter (PM) on cellular toxicity and oxidative stress by collecting PM10 samples in southern Italy and conducting biological assays. The results showed a strong correlation between carbon content and cellular oxidative stress and cytotoxicity, indicating the influence of combustion sources on the toxicological potential of PM10. Additionally, genotoxicity results were correlated with oxidative stress and cytotoxicity outcomes, with combustion sources playing a significant role in determining the biological effects of PM10.
Article
Biochemistry & Molecular Biology
Victor Pavlovich Chelomin, Valentina Vladimirovna Slobodskova, Sergey Petrovich Kukla, Andrey Alexandrovich Mazur, Nadezda Vladimirovna Dovzhenko, Avianna Fayazovna Zhukovskaya, Alexander Alexandrovich Karpenko, Maxim Alexandrovich Karpenko, Vyacheslav Sergeevich Odintsov
Summary: Marine and ocean water pollution from plastic waste poses a serious threat to future generations, but data on the toxicity of plastic particles are currently incomplete due to their chemical diversity. Organisms have different risks from plastic particles depending on their habitat and feeding habits. We conducted experiments on feeding gastropod scraping mollusk Littorina brevicula with polymethylmethacrylate (PMMA) and polytetrafluoroethylene (PTFE) particles. Results showed that PMMA exposure caused oxidative stress and DNA damage in digestive gland cells, while no similar changes were observed in mollusks fed with PTFE-containing food.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Una Stamenovic, Sladana Davidovic, Sandra Petrovic, Andreja Leskovac, Milovan Stoiljkovic, Vesna Vodnik
Summary: Two Ag-PANI/PVP nanocomposites with similar silver content but different morphologies were prepared by integrating silver nanoparticles during oxidative polymerization. These nanocomposites showed strong antimicrobial potential against indicator microbial strains, making them suitable for non-medical applications like wastewater treatment.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Ivana Cacciatore, Michele Ciulla, Rossella Grande, Valentina Puca, Iole Robuffo, Leila Chekir-Ghedira, Lisa Marinelli, Rayhane Ben Khalifa, Marilisa Pia Dimmito, Vincenzo De Laurenz, Antonio Di Stefano
Summary: This study aimed to improve the antibiotic activity of vancomycin hydrochloride (VAN) by formulating multiple lipid nanoparticles (MLNs) containing carvacrol (CAR) and VAN co-loaded (CAR-VAN-MLNs). The results showed that the drug-loaded MLNs had appropriate particle size, negative surface charges, and high encapsulation efficiencies for both CAR and VAN. The stability and sustained drug release of the formulations were confirmed, and the morphological analysis indicated that the MLNs had a spherical shape. The co-encapsulation strategy improved the antibiotic activity of VAN against Staphylococcus aureus.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Marina Mingoia, Carmela Conte, Annalisa Di Rienzo, Marilisa Pia Dimmito, Lorella Marinucci, Gloria Magi, Hasan Turkez, Maria Concetta Cufaro, Piero Del Boccio, Antonio Di Stefano, Ivana Cacciatore
Summary: This study reports the synthesis of novel cinnamic acid-based antimicrobials (DM1-11) and their potential as antibacterial drugs for the treatment of ESKAPE-related skin infections. DM2 shows significant antibacterial activity and wound healing properties, making it a potentially safe topical agent for wound treatment.
Article
Biochemistry & Molecular Biology
Rosalba Florio, Barbara De Filippis, Serena Veschi, Viviana di Giacomo, Paola Lanuti, Giulia Catitti, Davide Brocco, Annalisa di Rienzo, Amelia Cataldi, Ivana Cacciatore, Rosa Amoroso, Alessandro Cama, Laura De Lellis
Summary: Pancreatic cancer has limited response to current therapeutic options, so there is a need for more effective and low-toxic agents. In this study, the antiproliferative activities of newly synthetized resveratrol analogues in pancreatic cancer cell lines were explored. The most active compound exhibited marked antiproliferative effects through mechanisms involving DNA damage, apoptosis induction, and interference in cell cycle progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Emira D'Amico, Tania Vanessa Pierfelice, Rosa Amoroso, Ivana Cacciatore, Camillo D'Arcangelo, Stefania Lepore, Simonetta D'Ercole, Natalia Di Pietro, Annalisa Di Rienzo, Morena Petrini, Adriano Piattelli, Alessia Ricci, Susi Zara, Antonio Di Stefano, Giovanna Iezzi, Barbara De Filippis
Summary: Recently, research has focused on finding new approaches to manage oral wound healing. The use of resveratrol (RSV) as a drug is limited due to its poor bioavailability. This study aimed to investigate a series of RSV derivatives (1a-j) with better pharmacokinetic profiles. Among them, derivatives 1d and 1h showed increased cell viability compared to RSV. Further studies demonstrated that both 1d and 1h exhibited positive effects on cell viability, proliferation, and gene expression in various cells involved in oral wound healing. Additionally, 1d and 1h showed promising physicochemical properties and stability, indicating their potential as RSV-based agents for oral tissue repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Biochemistry & Molecular Biology
Antonio Di Stefano
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Sara Pagotto, Pasquale Simeone, Davide Brocco, Giulia Catitti, Domenico De Bellis, Simone Vespa, Natalia Di Pietro, Lisa Marinelli, Antonio Di Stefano, Serena Veschi, Laura De Lellis, Fabio Verginelli, Francesco Kaitsas, Manuela Iezzi, Assunta Pandolfi, Rosa Visone, Nicola Tinari, Ignazio Caruana, Mauro Di Ianni, Alessandro Cama, Paola Lanuti, Rosalba Florio
Summary: This review highlights the potential of CAR-T-derived extracellular vesicles (EVs) as therapeutic agents in tumors. CAR-T lymphocytes have shown potential effectiveness in immunotherapy against refractory neoplastic diseases. However, physical barriers, hostile microenvironment, and tumor heterogeneity limit their efficacy against solid tumors. The use of CAR-T-derived EVs as therapeutic agents may enhance CAR-T homing and overcome some adverse effects.
Article
Chemistry, Medicinal
Ivana Cacciatore, Sonia Spalletta, Annalisa Di Rienzo, Vincenzo Flati, Erika Fornasari, Laura Pierdomenico, Piero Del Boccio, Silvia Valentinuzzi, Erica Costantini, Elena Toniato, Stefano Martinotti, Carmela Conte, Antonio Di Stefano, Iole Robuffo
Summary: This study synthesized a series of carvacrol derivatives and found that one derivative, CD3, exhibited anti-obesity and anti-inflammatory effects.
Article
Health Care Sciences & Services
Michele Ciulla, Lisa Marinelli, Giuseppe Di Biase, Ivana Cacciatore, Fiorenzo Santoleri, Alberto Costantini, Marilisa Pia Dimmito, Antonio Di Stefano
Summary: This systematic study analyzes the differences between the European and American healthcare systems in terms of pharmaceutical market approach. It emphasizes the use of managed entry agreements (MEAs) in European countries, as opposed to the unregulated pharmaceutical prices in the American market. Data were collected from various sources, and a literature review was conducted to gather information from the past ten years. The comparison reveals the importance of market access regulation in reducing therapy costs and improving the efficiency, quality, and accessibility for patients.
Article
Biochemistry & Molecular Biology
Abdelmoujoud Faris, Ivana Cacciatore, Ibrahim M. Ibrahim, Mohammed H. AL Mughram, Hanine Hadni, Kamal Tabti, Menana Elhallaoui
Summary: The inhibition of JAK3 is crucial in developing treatments for autoimmune diseases. A newly discovered JAK3 protein, along with the prediction model, has shown promising results in identifying potential JAK3 inhibitors.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Otorhinolaryngology
Necati Ilhan, Ahmet Kara, Elvan Sahin, Mahmut Sinan Yilmaz, Mehmet Guven, Miyase Erdogan, Deniz Demir
Summary: This study evaluated the anti-adhesive effect of poly (dl-lactide epsilon-caprolactone) as a material in middle-ear surgeries. The results showed that poly (dl-lactide epsilon-caprolactone) had no toxic effects in the rat's middle ear cavity and prevented adhesion of the tympanic membrane.
JOURNAL OF INTERNATIONAL ADVANCED OTOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Niki Gouleni, Annalisa Di Rienzo, Ahmet Yilmaz, Harun Selvitopi, Mehmet Enes Arslan, Adil Mardinoglu, Hasan Turkez, Antonio Di Stefano, Stamatia Vassiliou, Ivana Cacciatore
Summary: In this study, a series of novel styryl-thiazole hybrids were designed, synthesized, and evaluated as potential candidates for the treatment of Alzheimer's disease. Among the synthesized hybrids, compounds 6e and 6i showed promising results in increasing cell viabilities and inhibiting AChE. Docking studies and in vitro assays further confirmed these findings. Compound 6i exhibited better physicochemical properties and demonstrated potential as a multi-targeted active molecular profile for treating AD.
RSC MEDICINAL CHEMISTRY
(2023)
Article
Oncology
Gamze Guney Eskiler, Nur Kazan, Ayten Haciefendi, Asuman Deveci Ozkan, Kayhan Ozdemir, Mirac Ozen, Havva Belma Kocer, Fahri Yilmaz, Suleyman Kaleli, Elvan Sahin, Cemil Bilir
Summary: The study aimed to compare the expression levels of receptor tyrosine kinases (RTKs) and AKT1 and mTOR in the exosomes of breast cancer (BC) patients before and after neoadjuvant chemotherapy (NACT) with healthy females. The results showed that FGFR2, PDGFRB, AKT1, and mTOR were significantly upregulated in BC patients before NACT compared to the healthy group, while PDGFRB and AKT1 were significantly down-regulated after NACT. PDGFRB expression level correlated with therapy response and tumor size.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2023)