Article
Biochemistry & Molecular Biology
Caterina Visconte, Chiara Fenoglio, Maria Serpente, Paola Muti, Andrea Sacconi, Marta Rigoni, Andrea Arighi, Vittoria Borracci, Marina Arcaro, Beatrice Arosio, Evelyn Ferri, Maria Teresa Golia, Elio Scarpini, Daniela Galimberti
Summary: This study isolated total EVs from plasma and characterized the miRNA contents in AD patients. The results showed widespread deregulation of specific miRNAs in plasma EVs, some of which were already known to be associated with neurological pathologies. Further validation analysis confirmed the upregulation of certain miRNAs in prodromal AD patients, suggesting their involvement in the early progression of AD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Chemical
Lara Ahmad, Chiara Demartini, Michele Corrado, Gloria Vaghi, Elisa Maria Piella, Marta Allena, Anna Maria Zanaboni, Rosaria Greco, Cristina Tassorelli, Roberto De Icco
Summary: Preliminary findings suggest that miRNAs play a role in chronic pain and migraine, with altered expression levels during migraine attacks. These miRNAs may serve as potential biomarkers for migraine diagnosis and treatment, but further research is needed to confirm their specificity and accuracy.
Article
Multidisciplinary Sciences
Swati Srivastava, Iti Garg, Yamini Singh, Ramesh Meena, Nilanjana Ghosh, Babita Kumari, Vinay Kumar, Malleswara Rao Eslavath, Sayar Singh, Vikas Dogra, Mona Bargotya, Sonali Bhattar, Utkarsh Gupta, Shruti Jain, Javid Hussain, Rajeev Varshney, Lilly Ganju
Summary: The outbreak of COVID-19 pandemic in December 2019 had a global impact on millions of people. This study aimed to identify specific biomarkers for predicting the severity of COVID-19 and formulating treatment strategies. Through bioinformatic analysis of a small cohort, 31 microRNAs associated with COVID-19 were identified.
Article
Biochemistry & Molecular Biology
Amna Al-Nesf, Nada Mohamed-Ali, Vanessa Acquaah, Maneera Al-Jaber, Maryam Al-Nesf, Mohamed A. Yassin, Nelson N. Orie, Sven Christian Voss, Costas Georgakopoulos, Rikesh Bhatt, Alka Beotra, Vidya Mohamed-Ali, Mohammed Al-Maadheed
Summary: Untargeted metabolomics was used to identify a panel of serum and urine metabolites as potential markers for autologous blood transfusion (ABT). This method has the potential to be universally adopted for detecting ABT.
Article
Sport Sciences
Andreas Breenfeldt Andersen, Jacob Bejder, Thomas C. Bonne, Henrik Sorensen, Helle Sorensen, Grace Jung, Tomas Ganz, Elizabeta Nemeth, Niels H. Secher, Par I. Johansson, Nikolai Baastrup Nordsborg
Summary: This study examined the use of hepcidin and erythroferrone (ERFE) as biomarkers for indirectly detecting autologous blood transfusion. The results showed that hepcidin and ERFE could complement the athlete biological passport (ABP) in detecting blood transfusion. Besides, the endurance performance of the participants improved after the transfusion.
MEDICINE & SCIENCE IN SPORTS & EXERCISE
(2022)
Article
Physiology
Ceren Eyileten, Zofia Wicik, Alex Fitas, Mikolaj Marszalek, Jenny E. Simon, Salvatore De Rosa, Szczepan Wiecha, Jeffrey Palatini, Marek Postula, Lukasz A. Malek
Summary: The study evaluated the expression of miRNAs in ultramarathon runners before and after the race, showing significant increases in miR-125a-5p, miR-126, and miR-223 post-race. MiR-1-3p showed a negative correlation with hs-CRP levels post-race and was associated with faster race completion times.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Immunology
Emilio Nunez-Borque, Sergio Fernandez-Bravo, Pablo Rodriguez Del Rio, Lucia Palacio-Garcia, Angela Di Giannatale, Virginia Di Paolo, Angela Galardi, Marta Colletti, Luisa Pascucci, Jaime Tome-Amat, Javier Cuesta-Herranz, Maria Dolores Ibanez-Sandin, Jose Julio Laguna, Alberto Benito-Martin, Vanesa Esteban
Summary: This study investigates the involvement of miRNAs in anaphylactic reactions and their potential as biomarkers. The researchers found differential expression profiles of circulating miRNAs in patients with anaphylaxis, particularly miR-375-3p. Functional and molecular experiments demonstrated that miR-375-3p plays a negative role in the stability of the endothelial barrier. These findings provide insights into the molecular mechanisms underlying anaphylactic reactions and suggest the potential of miR-375-3p as a biomarker.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Endocrinology & Metabolism
Nina Morup, Rytis Stakaitis, Ailsa Maria Main, Ieva Golubickaite, Casper P. Hagen, Anders Juul, Kristian Almstrup
Summary: During the transition to puberty in boys, both the levels and bioactivity of miR-30b-5p increase, suggesting its role in the activation of the HPG axis. The levels of miR-155-5p also increase, while miR-200b-3p does not consistently change.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Infectious Diseases
Mahmut Ulger, Mehmet Sami Serin, Seda Tezcan Ulger, Gonul Aslan, Ahmet Ilvan, Eyup Naci Tiftik, Gurol Emekdas
Summary: This study identified a correlation between altered expression levels of miRNAs in plasma and cytokine levels in tuberculosis patients, suggesting that miRNAs may serve as potential non-invasive biomarkers for early diagnosis of pulmonary tuberculosis.
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2022)
Article
Veterinary Sciences
Loubna Abou el Qassim, Jaime Alonso, Ke Zhao, Sandrine Le Guillou, Jorge Diez, Fernando Vicente, Manuel Fernandez-Sanjurjo, Eduardo Iglesias-Gutierrez, Leluo Guan, Luis J. Royo
Summary: This article explores the extensive milk production system based on molecular markers in milk and investigates its potential benefits for consumer health. Differences in miRNA levels were observed, suggesting that the production system type may influence the miRNA levels and functional potential of animal-origin agri-food products.
VETERINARY SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Jessica T. Hochberg, Aalam Sohal, Priya Handa, Bryan D. Maliken, Take-Kyun Kim, Kai Wang, Eric Gochanour, Yu Li, J. Bart Rose, James E. Nelson, Keith D. Lindor, Nicholas F. LaRusso, Kris V. Kowdley
Summary: This study investigated the changes in serum miRNA and cytokine profiles in patients with PSC treated with high-dose ursodeoxycholic acid (hd-UDCA) or placebo. The results showed significant alterations in serum miRNA profiles after hd-UDCA treatment, suggesting a potential link to liver toxicity.
Article
Biochemistry & Molecular Biology
Lui Ng, Ryan Wai-Yan Sin, David Him Cheung, Wai-Keung Leung, Abraham Tak-Ka Man, Oswens Siu-Hung Lo, Wai-Lun Law, Dominic Chi-Chung Foo
Summary: Non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease that is increasing worldwide, and it is associated with colorectal polyps. This study aimed to investigate the potential of serum microRNAs (miRNAs) in identifying NAFLD in colorectal polyp patients. The results showed that a serum miRNA panel composed of four miRNA pairs significantly identified NAFLD in colorectal polyp patients, and its performance improved when patients with other concurrent metabolic disorders were removed from the analysis. The serum miRNA panel could be a potential diagnostic biomarker for screening NAFLD in colorectal polyp patients for early diagnosis and prevention of disease progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Veterinary Sciences
Matias Aguilera-Rojas, Soroush Sharbati, Torsten Stein, Mario Candela Andrade, Barbara Kohn, Ralf Einspanier
Summary: This study investigated the effects of haemolysis on circulating miRNAs in dogs, and found that some miRNAs were significantly sensitive to haemolysis. The study also revealed differences in canine miRNA abundance compared to human miRNA. These findings have important implications for the discovery of circulating biomarkers in dogs, and emphasize the need to carefully consider the impact of haemolysis in serum or plasma-based tests.
BMC VETERINARY RESEARCH
(2022)
Article
Endocrinology & Metabolism
Alessia Dattilo, Giovanni Ceccarini, Gaia Scabia, Silvia Magno, Lara Quintino, Caterina Pelosini, Guido Salvetti, Roberto Cusano, Matteo Massidda, Lucia Montanelli, Donatella Gilio, Gianluca Gatti, Alessandro Giacomina, Mario Costa, Ferruccio Santini, Margherita Maffei
Summary: Lipodystrophy (LD) is a rare group of disorders characterized by the loss of white adipose tissue (WAT) and metabolic disturbances. This study found that cmiRs-320 may serve as novel biomarkers for LD, with cmiR-320a-3p abundance inversely associated with indicators of WAT function, and downregulation of cmiR-320d predicting altered liver function and increased inflammation.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Endocrinology & Metabolism
Xiyao Yang, Na Wu
Summary: This review summarizes the roles and potential of exosomal miRNAs and circulating miRNAs in GDM. Changes in the expression and secretion of exosomal miRNAs can be used as diagnostic and prognostic markers for GDM. Additionally, exosomal miRNAs can target metabolic pathways, leading to insulin resistance.
DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY
(2022)
Article
Cell Biology
Matteo Zurlo, Francesco Nicoli, Davide Proietto, Beatrice Dallan, Cristina Zuccato, Lucia Carmela Cosenza, Jessica Gasparello, Chiara Papi, Elisabetta d'Aversa, Monica Borgatti, Chiara Scapoli, Alessia Finotti, Roberto Gambari
Summary: Inhibitors of mTOR, such as Sirolimus, can enhance vaccine responses in the elderly. Testing Sirolimus and other geroprotective drugs might be a key strategy to improve the overall health resilience of aged populations. This study analyzed the activity of CD4(+) and CD8(+) T cells in beta-Thalassemia patients treated with Sirolimus and found that it positively impacted the biological activity and number of memory T cells.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Valentino Bezzerri, Valentina Gentili, Martina Api, Alessia Finotti, Chiara Papi, Anna Tamanini, Christian Boni, Elena Baldisseri, Debora Olioso, Martina Duca, Erika Tedesco, Sara Leo, Monica Borgatti, Sonia Volpi, Paolo Pinton, Giulio Cabrini, Roberto Gambari, Francesco Blasi, Giuseppe Lippi, Alessandro Rimessi, Roberta Rizzo, Marco Cipolli
Summary: As an inherited disorder characterized by severe pulmonary disease, cystic fibrosis could be considered a comorbidity for coronavirus disease 2019. However, current clinical evidence suggests that cystic fibrosis may actually protect against SARS-CoV-2 infection. The expression of SARS-CoV-2 receptors in primary airway epithelial cells is regulated by the CFTR channel, and dysfunctional CFTR channels in cystic fibrosis cells result in reduced viral entry and replication.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Rita Canella, Federica Brugnoli, Mariana Gallo, Jeffrey W. Keillor, Anna Terrazzan, Elena Ferrari, Silvia Grassilli, Eric W. J. Gates, Stefano Volinia, Valeria Bertagnolo, Nicoletta Bianchi, Carlo M. Bergamini
Summary: This study investigates the role of transglutaminase 2 (TG2) within the cell and its interaction with the voltage-dependent Kv10.1 K+ channel, which is an important target in breast cancer therapies. The use of TG2 inhibitors selectively affects the membrane current of triple-negative cells. The findings highlight the interference of TG2 inhibitors with the Kv10.1 channel as a potential therapeutic tool depending on the specific features of cancer cells.
Article
Biochemistry & Molecular Biology
Giovanni Piva, Anna Crepaldi, Nicola Lamberti, Lorenzo Caruso, Natascia Rinaldo, Roberto Manfredini, Pablo Jesus Lopez-Soto, Vincenzo Gasbarro, Fabio Manfredini, Alda Storari
Summary: This observational study aimed to monitor the 5-year trends of kidney function in patients with peripheral artery disease (PAD) and concomitant chronic kidney disease (CKD) enrolled or not enrolled into a rehabilitative exercise program. The results showed that patients in the exercise program had improved kidney function and better clinical outcomes compared to those receiving usual care.
Article
Sport Sciences
Natascia Rinaldo, Giovanni Piva, Suzanne Ryder, Anna Crepaldi, Alba Pasini, Lorenzo Caruso, Roberto Manfredini, Sofia Straudi, Fabio Manfredini, Nicola Lamberti
Summary: Despite progress, gender bias still exists in scientific publication authorship. This study examines trends in authorship by gender in the field of exercise sciences and rehabilitation in the last 5 years. The results show that while women are almost equally represented as first authors, bias against women remains in the last authorship position, regardless of geographical area and journal ranking.
JOURNAL OF FUNCTIONAL MORPHOLOGY AND KINESIOLOGY
(2023)
Article
Health Care Sciences & Services
Nicola Lamberti, Luca Traina, Caterina Savrie, Elpiniki Tsolaki, Natascia Rinaldo, Sofia Straudi, Franco Guerzoni, Nicola Napoli, Roberto Manfredini, Vincenzo Gasbarro, Fabio Manfredini
Summary: This study evaluated the sex-specific difference in response to an exercise program among patients with peripheral artery disease and claudication. The results showed that women had a significantly higher 7-year survival rate and lower hospitalization and amputation rates in the exercise group compared to the control group. Therefore, active participation in a home-based pain-free exercise program can reduce the risk of adverse clinical outcomes and improve long-term outcomes, especially for women in PAD patients.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Biology
Fabio Casciano, Nicoletta Bianchi, Mirta Borin, Vittorio Vellani, Paola Secchiero, Carlo M. Bergamini, Simona Capsoni, Angela Pignatelli
Summary: We demonstrated for the first time that fluorescence intensity correlates very well with the expression of genes typical of different stages of maturation in dopaminergic neurons. We propose that the FACS method used to isolate these neurons may be used to engineer new neurons for therapeutic purposes in widespread pathologies, such as Parkinson's disease.
Article
Chemistry, Analytical
Antonino Casile, Giulia Fregna, Vittorio Boarini, Chiara Paoluzzi, Fabio Manfredini, Nicola Lamberti, Andrea Baroni, Sofia Straudi
Summary: This study investigated the use of the cost-effective Oculus Quest head-mounted display as a tool for assessing markerless hand motion capabilities in stroke patients, and found that it provided accurate and sensitive measurements comparable to a commercial marker-based system.
Article
Health Care Sciences & Services
Anna Crepaldi, Lorenzo Caruso, Giovanni Piva, Luca Traina, Vincenzo Gasbarro, Roberto Manfredini, Nicola Lamberti, Natascia Rinaldo, Fabio Manfredini, Pablo Jesus Lopez-Soto
Summary: This study aimed to detect foot perfusion changes in PAD patients after a home-based exercise program using infrared thermography. The results showed that after 20 weeks of exercise, foot temperature in both limbs significantly increased, with no significant difference between genders. Additionally, the ankle brachial index and performance also significantly improved over time.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Review
Biology
Angelo Aquino, Nicoletta Bianchi, Anna Terrazzan, Ornella Franzese
Summary: The dysregulations of PKC in tumors have made them natural targets for anticancer therapy, but the development of PKC modulators has not resulted in the expected therapeutic benefits. This may be due to the complex biological activities and mutations associated with PKC isoenzymes. This review focuses on PKC alterations associated with complete loss of function, the limited success in targeting PKC in certain cancers, and the negative clinical outcomes. Importantly, it emphasizes the need for more selective molecules and a better understanding of immune mechanisms controlled by PKC isoforms.
Review
Sport Sciences
Natascia Rinaldo, Alba Pasini, Sofia Straudi, Giovanni Piva, Anna Crepaldi, Andrea Baroni, Lorenzo Caruso, Fabio Manfredini, Nicola Lamberti
Summary: People with multiple sclerosis (pwMS) experience decreased bone mineral density and worsening body composition, negatively impacting their quality of life. This systematic review and meta-analysis examined the effects of nonpharmacological interventions, such as physical activity, nutrition, and rehabilitation, on body composition (BC) and bone mineral density (BMD) in pwMS. The results showed inconsistent positive effects on BC, but high-intensity training and ketogenic diets appeared to be promising interventions. Limited studies considered BMD, and the findings were inconclusive. Further research is needed to confirm these results.
JOURNAL OF FUNCTIONAL MORPHOLOGY AND KINESIOLOGY
(2023)
Article
Respiratory System
Nicola Lamberti, Giovanni Piva, Yuri Battaglia, Michele Franchi, Matteo Pizzolato, Antonio Argentoni, Giorgio Gandolfi, Giulia Gozzi, Margherita Lembo, Pietro Lavisci, Alda Storari, Natascia Rinaldo, Fabio Manfredini, Annalisa Cogo
Summary: This study aimed to investigate the feasibility and effectiveness of low-intensity respiratory muscle training in end-stage kidney disease patients. The results showed that home-based RMT significantly improved respiratory muscle strength in patients.
ADVANCES IN RESPIRATORY MEDICINE
(2023)
Article
Oncology
Jessica Gasparello, Chiara Papi, Matteo Zurlo, Laura Gambari, Alex Manicardi, Andrea Rozzi, Matteo Ferrarini, Roberto Corradini, Roberto Gambari, Alessia Finotti
Summary: Starting from a list of nine dysregulated miRNAs in early-stage CRC patients, the study verified the therapeutic effects of anti-miRNA PNAs targeting the most relevant up-regulated miRNAs. PNAs down-regulated these target miRNAs and induced apoptosis in CRC cellular models. Combining anti-miRNA PNAs with pro-apoptotic compounds like sulforaphane resulted in high levels of apoptosis induction.
Article
Biochemistry & Molecular Biology
Cristina Zuccato, Lucia Carmela Cosenza, Matteo Zurlo, Giulia Breveglieri, Nicoletta Bianchi, Ilaria Lampronti, Jessica Gasparello, Chiara Scapoli, Monica Borgatti, Alessia Finotti, Roberto Gambari
Summary: The human homologue of mouse Ly-1 antibody reactive clone protein (LYAR) is a potential regulator of gamma-globin gene transcription. The LYAR rs368698783 (G > A) polymorphism is present in beta-thalassemia patients and reduces the LYAR binding efficiency to the A gamma-globin gene. This study aimed to stratify beta-thalassemia patients based on the rs368698783 (G > A) polymorphism and investigate their erythroid precursor cells' response to selected HbF inducers in vitro.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)