Article
Biotechnology & Applied Microbiology
Satoshi Mizoguchi, Tomoshi Tsuchiya, Ryoichiro Doi, Tomohiro Obata, Mayumi Iwatake, Shintaro Hashimoto, Hirotaka Matsumoto, Hiroshi Yukawa, Hiroko Hayashi, Tao-Sheng Li, Kazuko Yamamoto, Keitaro Matsumoto, Takuro Miyazaki, Koichi Tomoshige, Takeshi Nagayasu
Summary: Two-dimensional cell cultures have played a significant role in lung cancer research, but there is increasing interest in 3D cultures as a more efficient and effective research model. This study successfully created an ex vivo lung cancer model based on bioengineered lungs, which closely replicated the 3D structure and tumor microenvironment of actual lungs. The results demonstrated the superiority of this model and its potential use in lung cancer research and pathophysiological studies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Arvind Manikantan Padma, Ahmed Baker Alsheikh, Min Jong Song, Randa Akouri, Levent M. Akyurek, Mihai Oltean, Mats Brannstrom, Mats Hellstrom
Summary: The study investigated the immune response after engraftment of three decellularized scaffold types in rats. One scaffold triggered an early immune response which stabilized after 30 days, another showed a delayed immune response negative for scaffold survival, while the third remained least immunogenic and similar to autologous tissue implants. A mild detergent-based decellularization protocol was found advantageous and promising for future uterus bioengineering applications.
BIOMEDICAL MATERIALS
(2021)
Article
Cell & Tissue Engineering
Arvind Manikantan Padma, Ahmed Baker Alshaikh, Min Jong Song, Randa Akouri, Mihai Oltean, Mats Brannstrom, Mats Hellstrom
Summary: This study investigated the immune response after transplantation of decellularized tissue and explored the impact of different decellularization protocols on the immune response. The findings suggest that scaffolds from certain protocols may induce transient or early pro-inflammatory responses, and Protocol 2 was identified as the least immunogenic for future uterus bioengineering applications.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Review
Medicine, Research & Experimental
Maryam Asadi, Mostafa Khalili, Hajie Lotfi, Sevil Vaghefi Moghaddam, Nosratollah Zarghami, Helder Andre, Effat Alizadeh
Summary: Development of novel technologies in tissue engineering has led to the exploration of scaffold-based and scaffold-free tissues for liver regeneration. Innovations such as cell sheet technology and analogs of liver acellular extracellular matrix have shown promise in aiding liver repair and regeneration.
Article
Biotechnology & Applied Microbiology
Aylin Acun, Ruben Oganesyan, Maria Jaramillo, Martin L. Yarmush, Basak E. Uygun
Summary: End-stage liver diseases result in high mortality rates, and patients rely on liver transplantation for treatment, which is limited by donor scarcity and long waiting times. This study proposes the decellularization of non-transplantable livers as a potential solution to organ scarcity. By introducing induced pluripotent stem cells (iPSCs) into decellularized rat liver scaffolds, the exposure of iPSCs to the native liver microenvironment is maximized, resulting in enhanced differentiation into hepatocyte-like cells with higher maturity and functionality. This study emphasizes the crucial role of the native 3D liver microenvironment in the development of mature human-origin hepatocyte-like cells.
BIOENGINEERING-BASEL
(2022)
Article
Cell & Tissue Engineering
Wonwoo Jeong, Min Kyeong Kim, Hyun-Wook Kang
Summary: The type of detergent used for decellularization has a significant impact on the performance of decellularized extracellular matrix-based bio-inks, with TXA-based bio-ink showing the highest ECM content and best overall performance, including improved gelation kinetics, intermolecular bonding, mechanical properties, and 2D/3D printability. Cytocompatibility tests using primary mouse hepatocytes also demonstrated that the TXA-based dECM bio-ink led to increased albumin secretion and cytochrome P450 activity compared to other bio-inks.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Engineering, Biomedical
Colton Kostelnik, Julia Hohn, Carlos E. Escoto-Diaz, Jesse B. Kooistra, Matthew Stern, Derrick E. Swinton, William Richardson, Wayne Carver, John Eberth
Summary: Engineered replacement materials have great potential in vascular applications with a focus on optimizing decellularization processes for xenogeneic vascular tissue sources. Different anionic detergent concentrations were investigated for their ability to effectively remove cell proteins while leaving DNA content mostly intact. Longer treatment times improved decellularization by significantly reducing DNA content, but also led to reduced laminin content and altered mechanical properties of the vessels.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Ali Doryab, Otmar Schmid
Summary: Chronic lung diseases are a leading cause of death worldwide, and lung transplantation is currently the only treatment option but limited by donor availability. Lung tissue engineering offers a promising approach to regenerate damaged lung tissue. Researchers have developed an extracellular matrix-integrated scaffold that closely mimics the microenvironment of lung cells and shows potential for lung tissue engineering applications.
Article
Biochemistry & Molecular Biology
Tosha Mae Manalastas, Nathaniel Dugos, Gliceria Ramos, John Martin Mondragon
Summary: Combining perfusion decellularization with sonication significantly reduces the time needed to produce acellular scaffolds, but sonicator power has a significant effect on the microarchitecture integrity of the scaffold.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Evan T. Hoffman, Franziska E. Uhl, Loredana Asarian, Bin Deng, Chloe Becker, Juan J. Uriarte, Isaac Downs, Brad Young, Daniel J. Weiss
Summary: Chronic lung diseases, such as COPD and IPF, exhibit region-specific changes in extracellular matrix (ECM) composition, particularly in collagen and basement-membrane associated proteins. This study compares the ECM composition of whole decellularized lungs and specific anatomical lung regions in non-diseased, COPD, and IPF human lungs. The findings demonstrate distinct ECM signatures in different regions and reveal alterations in ECM composition specific to COPD and IPF, including enrichment of type-III collagen and fibulin in IPF aECM. This study provides methodology for future research and a dataset for identifying novel ECM targets for therapeutics.
Review
Engineering, Biomedical
Lillian Yuxian Lim, Shirley Suet Lee Ding, Padmalosini Muthukumaran, Swee Hin Teoh, Yexin Koh, Adrian Kee Keong Teo
Summary: Pancreas or islet transplantation is the reliable treatment for Type I diabetes patients, but the shortage of viable pancreata limits its use. Organ decellularization and recellularization offer a potential alternative source of donor organs. Studies on decellularization and recellularization of rodent, porcine, and human pancreata show promise for generating usable decellularized pancreas scaffolds. This review discusses the latest advances in pancreas decellularization, recellularization, and revascularization, as well as clinical considerations and future work needed for clinical translation of bioengineered pancreata.
ACTA BIOMATERIALIA
(2023)
Review
Physiology
Heather Wanczyk, Todd Jensen, Daniel J. Weiss, Christine Finck
Summary: Lung transplantation remains the main option for individuals with end-stage lung failure, but facing limitations such as a shortage of suitable donor lungs and immune rejection post-transplantation. To address these issues, research is being conducted on using decellularized biocompatible lung scaffolds reseeded with autologous lung cells for tissue regeneration. However, gaps still exist in understanding the reseeding methods of important cell populations and the spatiotemporal interactions between different cell populations necessary for proper lung growth and function.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Cell & Tissue Engineering
Gustavo Henrique DonaRodrigues Almeida, Rebeca Piatniczka Iglesia, Michelle Silva Araujo, Ana Claudia Oliveira Carreira, Erika Xavier dos Santos, Celso Vitor Alves Queiroz Calomeno, Maria Angelica Miglino
Summary: Tissue engineering is an innovative approach that has shown significant progress in rebuilding the uterus and its tissues in recent years to address reproductive issues. Research focuses on repair strategies, scaffold production, biomaterials, and animal models, contributing to advancements in regenerative medicine.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Luong Huu Dang, Yuan Tseng, How Tseng, Shih-Han Hung
Summary: A new procedure for the rapid partial decellularization of harvested trachea was developed in this study, resulting in low-immunogenic cartilage scaffolds with viable core cartilage cells that can achieve long-term survival after in vivo transplantation. The method showed potential in avoiding immune rejection and maintaining mechanical stability under stress-pressure testing.
Article
Cell & Tissue Engineering
Gustavo Henrique Dona Rodrigues Almeida, Rebeca Piatniczka Iglesia, Jaqueline de Carvalho Rinaldi, Mikaelly Kiemy Murai, Celso Vitor Alves Queiroz Calomeno, Leandro Norberto da Silva Junior, Bianca de Oliveira Horvath-Pereira, Leticia Beatriz Mazo Pinho, Maria Angelica Miglino, Ana Claudia Oliveira Carreira
Summary: Ovarian tissue engineering offers an innovative alternative to current methods for preserving and restoring ovarian fertility. Mimicking the ovarian extracellular matrix (ECM) is crucial for maintaining oocyte viability, and biomaterials that mimic ECM can provide conditions for cell anchorage, proliferation, and differentiation. This review highlights the main advances and challenges in this field, emphasizing the need for innovative methodologies in reproductive medicine.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Article
Surgery
Toshiaki Shichinohe, Eiji Kobayashi
Summary: The framework for cadaver surgical training (CST) in Japan was established in 2012 and is currently practiced in 33 medical schools and universities. The CST Promotion Committee of the JSS reviews and provides guidance for CST reports submitted by schools. In the future, an operating organization funded by stakeholders will be needed to sustain and oversee CST implementation.
Article
Immunology
Yatsumu Saito, Naoto Matsumoto, Shuichiro Yamanaka, Takashi Yokoo, Eiji Kobayashi
Summary: We generated interspecies chimeric renal organoids by adding heterologous rat renal progenitor cells to single cells from mouse fetal kidneys and observed their differentiation into renal tissues of three lineages. Chimeric renal organoids with a higher proportion of recipient cells showed reduced rejection compared to complete xenograft organoids.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Engineering, Multidisciplinary
Tsuyoshi Takamura, Kenji Matsui, Naoto Matsumoto, Yatsumu Saito, Toshinari Fujimoto, Susumu Tajiri, Shuichiro Yamanaka, Kei Matsumoto, Akimitsu Kobayashi, Izumi Yamamoto, Hiroshi Sasaki, Haruyuki Hirayama, Hitomi Matsunari, Kazuaki Nakano, Hiroshi Nagashima, Akihiko Kiyoshi, Takao Kuroda, Makoto Inoue, Takeshi Miyawaki, Takashi Yokoo, Eiji Kobayashi
Summary: This study compared the transplant rejection between neonatal and fetal pig kidneys in renal heterotransplantation. The results showed that fetal pig kidneys were spared from rejection despite the administration of the same immunosuppressive protocol to the monkeys and the recipient blood vessels flowing into the fetal kidneys.
Article
Oncology
Luis Filipe Azenha, Pietro Bertoglio, Peter Kestenholz, Michel Gonzalez, Matyas Pal, Thorsten Krueger, Bassam Redwan, Volkan Koesek, Eyad Al Masri, Takuro Miyazaki, Farahnaz Sadegh Beigee, Benedetta Bedetti, Philipp Schnorr, Joachim Schmidt, Patrick Zardo, Laura Boschetti, Sven Oliver Schumann, Fabrizio Minervini
Summary: Lung cancer is the leading cause of cancer-related death worldwide and accounts for about 40-50% of brain metastases. In early-stage lung cancer, the incidence of brain metastases is around 3%. The current international guidelines have different recommendations regarding brain imaging for early-stage cancer patients. Some studies suggest that surgical or radiosurgical treatment may improve survival, especially in asymptomatic patients. Advances in genome analysis have identified molecular targets for pharmaceutical agents. This multicentric study analyzed data from 577 patients with early-stage lung cancer who underwent brain imaging.
Review
Surgery
Eiji Kobayashi, Toshiaki Shichinohe
Summary: This article reports on the contents and conflicts of interest of Cadaver Surgical Training (CST) programs in Japan based on professional autonomy. The Japan Surgical Society CST Promotion Committee evaluated the validity of the reports from participating universities and provided advice on proper implementation of CST. Over the past decade, 38 universities have been using cadavers for clinical education and research, such as CST. In case of identified inappropriate entries or unclear areas, the universities could be asked to resubmit their reports. This review clarifies the evaluation points of the CST reporting system, presents the status and problems of its reporting, and provides suggestions for future dissemination of CST.
Editorial Material
Respiratory System
Takuro Miyazaki, Takeshi Nagayasu
JOURNAL OF THORACIC DISEASE
(2022)
Editorial Material
Orthopedics
Takane Suzuki, Toshiaki Shichinohe, Eiji Kobayashi
JOURNAL OF ORTHOPAEDIC SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Naoto Matsumoto, Shuichiro Yamanaka, Keita Morimoto, Kenji Matsui, Sandy Nishimura, Yoshitaka Kinoshita, Yuka Inage, Koki Fujimori, Takao Kuroda, Yatsumu Saito, Tsuyoshi Takamura, Toshinari Fujimoto, Susumu Tajiri, Kei Matsumoto, Makoto Inoue, Eiji Kobayashi, Takashi Yokoo
Summary: The number of patients with end-stage renal failure is increasing annually worldwide, but there is a shortage of renal transplantation donors. Transplanting renal progenitor cells into heterologous fetuses has been shown to induce the development of nephrons. In this study, researchers successfully generated human-mouse chimeric kidneys by combining this technology with drug-induced removal of specific renal progenitor cells in transgenic mice. They also developed a model using re-aggregation of mouse renal progenitor cells to evaluate the chimerogenic potential of human nephron progenitor cells derived from pluripotent stem cells. This study provides promising potential for the evaluation and generation of chimeric kidneys for regenerative medicine.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Immunology
Syuhei Yoshimoto, Akihiko Soyama, Masayuki Fukumoto, Takanobu Hara, Masaaki Hidaka, Shinji Torai, Hiroo Kasamatsu, Jun Ishikawa, Masayuki Ohara, Eiji Kobayashi, Susumu Eguchi
Summary: This study explored the feasibility of using livers from uncontrolled donors after circulatory death (u-DCD) through transplantation. A pig model simulating u-DCD was used, and liver function was evaluated after 24 hours of perfusion. The results showed that u-DCD livers can be successfully transplanted using ex vivo machine perfusion.
TRANSPLANTATION PROCEEDINGS
(2023)
Article
Immunology
Shinji Torai, Kanta Kurauchi, Eiji Kobayashi
Summary: This study presented a newly developed organ protector called OrganPocket and demonstrated its effectiveness in mitigating SWI injury in clinical kidney transplantation. The results showed that using OrganPocket significantly reduced organ temperature and alleviated SWI injury.
TRANSPLANTATION PROCEEDINGS
(2023)
Article
Immunology
Kentaro Ide, Hiroshi Sakai, Ryosuke Nakano, Yuki Imaoka, Naoki Tanimine, Ryuta Ide, Naofumi Tsukiyama, Kosuke Ono, Tetsuya Mochizuki, Ryosuke Arata, Keishi Hakoda, Koki Imaoka, Sotaro Fukuhara, Tomoaki Bekki, Hiroyuki Tahara, Masahiro Ohira, Eiji Kobayashi, Hideki Ohdan
Summary: This study examined the efficacy of a thermal barrier bag in kidney transplantation. The results showed that the bag effectively reduced the temperature of the transplanted kidney, leading to better preservation of kidney function and improved transplant outcomes.
TRANSPLANTATION PROCEEDINGS
(2023)
Article
Medicine, General & Internal
Kenji Matsui, Yoshitaka Kinoshita, Yuka Inage, Naoto Matsumoto, Keita Morimoto, Yatsumu Saito, Tsuyoshi Takamura, Hitomi Matsunari, Shuichiro Yamanaka, Hiroshi Nagashima, Eiji Kobayashi, Takashi Yokoo
Summary: Fetal porcine kidneys injected with human nephron progenitor cells could be a viable option for kidney transplantation; using a model of freezing and transplantation, we demonstrated that the differentiation potency of vitrified fetal porcine kidneys could be evaluated, providing a practical protocol to assess the quality of individual lots.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Transplantation
Kentaro Ide, Ryosuke Nakano, Yuki Imaoka, Hiroshi Sakai, Kosuke Ono, Naoki Tanimine, Hiroyuki Tahara, Masahiro Ohira, Keiko Ueda, Taizo Hirata, Eiji Kobayashi, Hideki Ohdan
Summary: The thermal barrier bag (TBB) can maintain a low temperature for transplanted kidneys during vascular anastomosis, leading to improved outcomes for kidney transplant recipients.
TRANSPLANTATION DIRECT
(2023)
Article
Engineering, Biomedical
Hidenori Tani, Eiji Kobayashi, Shinomi Yagi, Keisuke Tanaka, Kotaro Kameda-Haga, Shinsuke Shibata, Nobuko Moritoki, Kaworu Takatsuna, Taijun Moriwaki, Otoya Sekine, Tomohiko C. Umei, Yuika Morita, Yusuke Soma, Yoshikazu Kishino, Hideaki Kanazawa, Jun Fujita, Shunji Hattori, Shugo Tohyama
Summary: This study found that heart-derived collagen is more effective in heart tissue engineering and can lead to more mature engineered heart tissue.
Editorial Material
Biochemistry & Molecular Biology
Asahi Oda, Yoji Hakamata, Eiji Kobayashi
Summary: Blackberries contain important substances that have antioxidative and other effects, and efforts are being made to enhance their absorption in the body. Through studies using animal models, it has been found that pre-administration of blackberry extracts can have positive effects on the animals before an ischemic event.