Article
Multidisciplinary Sciences
Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Anita Franczak
Summary: This study revealed that dietary restriction during the peri-conceptional period causes changes in gene expression in the pig myometrium, particularly those involved in endocrine activity, embryo-maternal interactions, and the regulation of cell cycle and proliferation.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Malavika K. Adur, Jacob T. Seibert, Matthew R. Romoser, Katie L. Bidne, Lance H. Baumgard, Aileen F. Keating, Jason W. Ross
Summary: This study evaluated the impact of heat stress on the abundance of heat shock proteins (HSP) in the porcine endometrium, and investigated the regulatory effects of treatment methods on HSP. The results demonstrated significant changes in the abundance of HSP in the porcine endometrium in response to heat stress and progestin supplementation.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Reproductive Biology
Mehmet Kose, Mustafa Hitit, Mehmet Salih Kaya, Mesut Kirbas, Sukru Dursun, Ilyas Alak, Mehmet Osman Atli
Summary: This study aimed to elucidate the expression pattern of miRNAs in the endometrium during peri-implantation in ewes. A total of 116 ovine-specific miRNAs were detected, with 19 differentially expressed in early pregnancy. The differentially expressed miRNAs targeted a total of 315 genes, with functional enrichment in the innate immune system and signaling pathways.
Article
Biochemistry & Molecular Biology
Yuta Matsuno, Kazuya Kusama, Kazuhiko Imakawa
Summary: This study characterized the expression profile of lncRNAs during peri-implantation periods in sheep conceptuses. The analysis identified a total of 8808 lncRNAs, including 3423 novel lncRNAs. Gene ontology analysis revealed enrichment of lncRNA target genes in the respiratory electron transport chain (RETC). The findings suggest that lncRNAs may play a role in regulating RETC during conceptus development in sheep.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Reproductive Biology
Katherine M. Halloran, Claire Stenhouse, Robyn M. Moses, Heewon Seo, Gregory A. Johnson, Guoyao Wu, Fuller W. Bazer
Summary: Progesterone and interferon tau play important roles in the establishment and maintenance of pregnancy in ruminants. Agmatine and polyamines are crucial for the survival, growth, and development of mammalian conceptuses. This study shows that P4 and IFNT work together to regulate the expression of genes involved in the metabolism and transport of polyamines during the peri-implantation period of pregnancy in sheep.
BIOLOGY OF REPRODUCTION
(2022)
Article
Agriculture, Dairy & Animal Science
Sarah R. McCoski, Rebecca R. Cockrum, Alan D. Ealy
Summary: Exposure to maternal obesity during pregnancy can have significant effects on offspring development, which may not be apparent until adulthood. This study investigated how obesity influences gene expression in the endometrium during early pregnancy using a sheep model. The results showed that obesity affects gene expression related to the immune response, adhesion, and angiogenesis in the endometrium. These findings suggest that the peri-implantation endometrium is responsive to maternal obesity and changes in gene expression during early embryogenesis may contribute to developmental programming.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Immunology
Wen-Heng Ji, Dan-Dan Li, Dan-Ping Wei, Ai-Qin Gu, Ying Yang, Jing-Pian Peng
Summary: The study shows that CYP26A1 plays a significant role in regulating the polarization of uterine macrophages to M1, and deficiency of CYP26A1 can lead to insufficient M1 polarization during the peri-implantation period, affecting blastocyst implantation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Yanshe Xie, Guangbin Liu, Xupeng Zang, Qun Hu, Chen Zhou, Yaokun Li, Dewu Liu, Linjun Hong
Summary: The study revealed that uterine fluids' EVs contain various small RNAs that may be vital in the formation of a receptive endometrium and embryo implantation.
Article
Biotechnology & Applied Microbiology
Chen Zhou, Xinyan Cheng, Fanming Meng, Yongzhong Wang, Wanyun Luo, Enqin Zheng, Gengyuan Cai, Zhenfang Wu, Zicong Li, Linjun Hong
Summary: This study analyzed the circRNA expression profile in the pig endometrium during the 12th day of gestation and found that circRNAs are abundantly expressed and play important regulatory roles in related genes. These findings provide insights into the molecular mechanisms contributing to pregnancy establishment and embryo loss in pigs, as well as the molecular pathway differences between breeds during the critical implantation period.
Article
Reproductive Biology
Mohammed Salem Moqbel, Saeed Yaseen Al-Ramadan, Abdulrhman Khalid Al-haider, Thnaian Ali Althnaian, Robert C. Burghardt
Summary: Pregnancy is primarily established and maintained in the left uterine horn in camels, with pregnancies in the right horn resulting in embryonic death. The study focused on the expression of osteopontin (OPN) during the peri-implantation period, which plays a role in embryo adhesion, implantation, and placentation. The research found that OPN expression significantly increased in both uterine horns, but was higher in the left horn. OPN mRNA and protein were detected and upregulated during peri-implantation, with a greater intensity in the left uterine horn.
Article
Agriculture, Dairy & Animal Science
Yueshuai Liu, Hongxiang Ding, Yuze Yang, Yan Liu, Xin Cao, Tao Feng
Summary: This study investigated the effects of progesterone treatment on apoptosis and steroidogenesis in porcine placental trophoblasts and explored the underlying molecular mechanisms. The results showed that high doses of progesterone inhibited trophoblast proliferation and significantly affected the expression of apoptosis-related and steroidogenesis-related genes and proteins. Low doses of progesterone had a milder effect. The study contributes to the understanding of the effects of progesterone on porcine placental trophoblast functions, which are crucial for pregnancy and maternal-fetal health.
Article
Endocrinology & Metabolism
Rulan Bai, Kazuya Kusama, Yuta Matsuno, Hanako Bai, Toshihiro Sakurai, Koji Kimura, Kazuhiko Imakawa
Summary: In this study, it was found that prostaglandin E2 (PGE2) activates the cyclic AMP (cAMP) signaling pathway in bovine endometrial stromal cells, leading to the upregulation of early pregnancy-related genes. The activation of cAMP resulted in the upregulation of transcripts related to transcription regulation, nucleus, and mitotic spindle organization. The transcription factors NFIL3, CEBPA, and HIF1A were found to be regulated by cAMP and involved in the expression of PTGS1/2 and IGFBP1/3. Furthermore, NFIL3 and CEBPA were localized in the endometrial stroma on day 17 of pregnancy, but not on day 17 of the estrous cycle.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Pawel Jozef Wydorski, Anita Franczak
Summary: Electromagnetic fields can alter the expression levels of CYP19A3 and HSD17B4 in pig myometrium during the peri-implantation period, while progesterone may mitigate the effects of EMF, regulating the expression levels of aromatase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Ruofan Qi, Tao Zhang, Yingying Zhang, Jacqueline Pui Wah Chung, Wen-Jui Yang, Chi Chiu Wang
Summary: The aim of this study was to investigate the differences in local angiotensin II (AngII) and its receptors (AT1R, AT2R) in the endometrium and their correlation with microvessel density in women with reproductive failure. The results showed that compared to fertile women, the expression of AT2R was significantly lower and the AT1R/AT2R expression ratio was significantly higher in the stroma of women with recurrent implantation failure. The endometrium arteriole microvessel density was significantly lower and negatively correlated with the AT1R/AT2R expression ratio in the stroma of women with recurrent implantation failure. The study concludes that the local renin-angiotensin system dysregulation is associated with abnormal angiogenesis in women with recurrent implantation failure.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Runan Hu, Yanjing Huang, Yufan Song, Xiao Wu, Kunkun Song, Guangying Huang, Mingmin Zhang, Haoxu Dong
Summary: This study aimed to investigate the effect of acupuncture on improving the pregnancy rate of COH rats by regulating the opening time of the implantation window and endometrial receptivity. Acupuncture was performed on COH rats at specific acupoints for 7 times. The results showed that acupuncture significantly increased the pregnancy rate, restored abnormal hormone levels, and reversed the advancement of implantation window in COH rats. Additionally, acupuncture restored the expression levels of ER, PR, LIF, integrin beta 3, VEGF, and FGF-2 in the endometrium to varying degrees. Therefore, acupuncture may enhance endometrial receptivity and improve the pregnancy rate of COH rats.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Reproductive Biology
Nurul Akmal Jamaludin, Lisa Marie Thurston, Krzysztof Jan Witek, Albany Meikle, Shaghayegh Basatvat, Sarah Elliott, Stuart Hunt, Aneta Andronowska, Alireza Fazeli
Article
Agriculture, Dairy & Animal Science
Krzysztof Jan Witek, Adam J. Ziecik, Izabela Malysz-Cymborska, Aneta Andronowska
REPRODUCTION IN DOMESTIC ANIMALS
(2020)
Article
Agriculture, Dairy & Animal Science
A. Zlotkowska, A. Andronowska
DOMESTIC ANIMAL ENDOCRINOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Mohammad Mehedi Hasan, Janeli Viil, Freddy Lattekivi, James Ord, Qurat Ul Ain Reshi, Kersti Jaager, Agne Velthut-Meikas, Aneta Andronowska, Ulle Jaakma, Andres Salumets, Alireza Fazeli
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Reproductive Biology
Keerthie Dissanayake, Monika Nomm, Freddy Lattekivi, Yosra Ressaissi, Kasun Godakumara, Arina Lavrits, Getnet Midekessa, Janeli Viil, Rikke Baek, Malene Moller Jorgensen, Sourav Bhattacharjee, Aneta Andronowska, Andres Salumets, Ulle Jaakma, Alireza Fazeli
Article
Chemistry, Multidisciplinary
Getnet Midekessa, Kasun Godakumara, James Ord, Janeli Viil, Freddy Lattekivi, Keerthie Dissanayake, Sergei Kopanchuk, Ago Rinken, Aneta Andronowska, Sourav Bhattacharjee, Toonika Rinken, Alireza Fazeli
Article
Biochemistry & Molecular Biology
Ilmatar Rooda, Mohammad Mehedi Hasan, Kristine Roos, Janeli Viil, Aneta Andronowska, Olli-Pekka Smolander, Ulle Jaakma, Andres Salumets, Alireza Fazeli, Agne Velthut-Meikas
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Reproductive Biology
Coline Mahe, Aleksandra Maria Zlotkowska, Karine Reynaud, Guillaume Tsikis, Pascal Mermillod, Xavier Druart, Jennifer Schoen, Marie Saint-Dizier
Summary: After mating or insemination, the region-specific and hormonally regulated morphology and secretions of the utero-tubal junction and oviduct lead to the selection of a limited sub-population of top quality spermatozoa at the site of fertilization.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biology
Mohammad Mehedi Hasan, Qurat Ul Ain Re, Freddy Lattekivi, Janeli Viil, Kasun Godakumara, Keerthie Dissanayake, Aneta Andronowska, Ulle Jaakma, Alireza Fazeli
Summary: Before the union of an egg and spermatozoon, multiple vital processes occur in the female reproductive system. The maturation of spermatozoa in the female reproductive tract is crucial for fertilization. Follicular fluid (FF) and its extracellular vesicles (EVs) play a significant role in supporting sperm functions, with specific effects based on species and source of FF EVs. This study demonstrates that bovine FF EVs can support sperm function and contribute to a favorable periconceptional microenvironment.
Article
Biochemistry & Molecular Biology
Freddy Lattekivi, Irina Guljavina, Getnet Midekessa, Janeli Viil, Paul R. Heath, Rikke Baek, Malene Moller Jorgensen, Aneta Andronowska, Kulli Kingo, Alireza Fazeli
Summary: Psoriasis vulgaris (PsV) and psoriatic arthritis (PsA) are inflammatory diseases with unresolved pathophysiological aspects. In this study, the miRNA contents and surface proteome of blood serum extracellular vesicles (EVs) were compared between PsV and PsA patients and healthy controls. It was found that there were differentially enriched EV-bound miRNAs in both PsV and PsA groups, suggesting that EVs may play a role in the pathophysiological processes of PsV and PsA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Coline Mahe, Regis Lavigne, Emmanuelle Com, Charles Pineau, Yann Locatelli, Aleksandra Maria Zlotkowska, Carmen Alminana, Guillaume Tsikis, Pascal Mermillod, Jennifer Schoen, Marie Saint-Dizier
Summary: Understanding the composition of oviduct fluid is crucial for understanding the microenvironment of sperm capacitation, fertilization, and early embryo development. This study analyzed the proteome of oviduct fluid samples from different regions, stages, and sides of the oviduct, and found that protein abundance was mainly influenced by the oviduct region, followed by the proximity of the ovulating ovary and the peri-ovulatory stage.
SCIENTIFIC REPORTS
(2022)
Article
Veterinary Sciences
Johanna Piibor, Keerthie Dissanayake, Getnet Midekessa, Aneta Andronowska, Ants Kavak, Andres Waldmann, Alireza Fazeli
Summary: A comparison was made between the follicular and luteal phases of the oestrous cycle in bovine uterine fluid (UF), revealing differences in the concentration and protein profile of extracellular vesicles (EV). These findings suggest that EV modulate the uterine microenvironment throughout the oestrous cycle.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Qurat Ul Ain Reshi, Kasun Godakumara, James Ord, Keerthie Dissanayake, Mohammad Mehedi Hasan, Aneta Andronowska, Paul Heath, Alireza Fazeli
Summary: Spermatozoa can alter the gene expression in female reproductive tract epithelial cells both remotely and through contact, and can also change the cargo of extracellular vesicles derived from these cells, which may play an important role in pre and post-fertilization events.
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2023)
Meeting Abstract
Agriculture, Dairy & Animal Science
A. Zlotkowska, A. Andronowska
REPRODUCTION IN DOMESTIC ANIMALS
(2020)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.