Journal
TALANTA
Volume 199, Issue -, Pages 324-328Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2019.02.080
Keywords
Electrochemical assay; DNA oxidative damage; 8-hydroxy-2'-deoxyguanosine; Aptamer; DNAzyme
Categories
Funding
- National Natural Science Foundation of China [81672570, 81772593]
- China Postdoctoral Science Foundation [2017M611532]
Ask authors/readers for more resources
In this work, an electrochemical method for sensitive analysis of 8-hydroxy-2'-deoxyguanosine, a key biomarker that is widely used to study oxidative injury-related diseases, is proposed based on exonuclease-mediated functional nucleic acid. In the design, exonuclease can not only distinguish the existence of target, but also suppress the background noise, thus the sensitivity can be enhanced. Moreover, DNAzyme designed in the functional nucleic acid can further improve the sensitivity of the analysis during signal generation process. Therefore, exonuclease-mediated functional nucleic acid may ensure high sensitivity of the assay. Further studies reveal that the detection of 8-hydroxy-2'-deoxyguanosine can be achieved with a linearity from 0.01 nM to 7.0 M and a detection limit of 6.82 pM. The new method has also been successfully applied to the determination of 8-OHdG in urine with good results, indicating its great potential for practical use.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available