Article
Biophysics
Junping Wen, Hongchao Gou, Siyuan Wang, Qijie Lin, Kaifeng Chen, Yuqian Wu, Xuehuan Huang, Haiyan Shen, Xiaoyun Qu, Jianhan Lin, Ming Liao, Jianmin Zhang
Summary: In this study, a novel genotyping method called CACA was proposed, which utilizes smartphone-assisted visualization to enhance the efficiency of specific pathogen identification and detection. The method has low detection limits and high accuracy, and when combined with a portable hand-warmer, it achieves power-free testing.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Guoxia Zheng, Qian Gao, Youwei Jiang, Ling Lu, Jianfeng Li, Xingcai Zhang, Hongyu Zhao, Panpan Fan, Yutong Cui, Furong Gu, Yunhua Wang
Summary: By combining digital microfluidic (DMF) with loop-mediated isothermal amplification (LAMP), an automated instrument-compact DMF-LAMP device was developed for rapid and sensitive detection of Vibrio parahaemolyticus. Proper mixing was found to enhance the amplification reaction rate, shorten the amplification time, and improve detection sensitivity of the device. The device showed high sensitivity with a detection limit of <0.23 x 10(3) CFU/g, making it a promising sample-to-answer system for quick and sensitive analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Environmental
Juxiang Liu, Ping Chen, Xulong Hu, Liping Huang, Zhi Geng, Hao Xu, Wenjun Hu, Lin Wang, Ping Wu, Gang L. Liu
Summary: The development of a nanoplasmonic enhanced isothermal amplification (NanoPEIA) method provides a fast and high-throughput detection platform for SARS-CoV-2. This method combines a nanoplasmonic sensor with isothermal amplification and can complete the entire detection process within a short time. It shows high sensitivity and specificity for detecting the N and orflab genes of SARS-CoV-2, making it suitable for POCT diagnosis in resource-poor and highly populated areas.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Ian T. Hull, Enos C. Kline, Gaurav K. Gulati, Jack Henry Kotnik, Nuttada Panpradist, Kamal G. Shah, Qin Wang, Lisa Frenkel, James Lai, Joanne Stekler, Barry R. Lutz
Summary: The study presents a novel quantitative detection method for HIV DNA or RNA using a combination of recombinase polymerase amplification and lateral flow detection technologies, allowing for rapid and accurate testing in low-resource healthcare settings, with the potential to improve the quality of HIV treatment.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Qiaoqiao Zhou, Jiangfei Pan, Laiming Mo, Zhaofan Luo, Zhifeng Qin, Zong Dai, Changqing Yi
Summary: This study developed a cost-effective, portable, and easy-to-use on-site genetic diagnostics sensing system for pathogen screening. The system consists of a paper-based biochip and a smartphone-based portable device, which together enable simultaneous detection of multiple pathogens and have demonstrated practicality in foodborne pathogen detection.
Article
Chemistry, Analytical
Hongchao Gou, Qijie Lin, Haiyan Shen, Kaiyuan Jia, Yucen Liang, Junhao Peng, Chunhong Zhang, Xiaoyun Qu, Yanbin Li, Jianhan Lin, Jianmin Zhang, Ming Liao
Summary: A novel linear displacement isothermal amplification (LDIA) method was developed for specific gene detection, which has advantages of simple primer design, high sensitivity and specificity, and applicability for point-of-care testing (POCT).
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemical Research Methods
Ruben R. G. Soares, Ahmad S. Akhtar, Ines F. Pinto, Noa Lapins, Donal Barrett, Gustaf Sandh, Xiushan Yin, Vicent Pelechano, Aman Russom
Summary: This study aimed to develop a cost-effective viral load detection system for point-of-care COVID-19 diagnostics. By developing an integrated modular centrifugal microfluidic platform, rapid amplification and detection of viral RNA from nasopharyngeal swab samples with high specificity and sensitivity were achieved.
Article
Biophysics
Ting Zheng, Xianming Li, Yanjun Si, Minjin Wang, Yuzhen Zhou, Yusheng Yang, Na Liang, Binwu Ying, Peng Wu
Summary: In this study, an accurate point-of-care testing (POCT) platform was proposed by specific recognition of target amplicons with peptide nucleic acid (PNA, assisted by T7 Exonuclease), which efficiently eliminated false positives and showed high sensitivity and specificity for detecting SARS-CoV-2.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Physical
Jianping Liang, Jie Zeng, Xiaojuan Huang, Tengteng Zhu, Yonglong Gong, Chen Dong, Xiangrong Wang, Lingzhi Zhao, Lei Xie, Kang Liang, Qiongxiang Tan, Yali Cui, Biao Kong, Wenli Hui
Summary: With the increasing global threat of diseases and infections, the development of a fast, low-cost, and easy-to-use point-of-care testing system is essential. This study proposes a rapid visual detection method that integrates loop-mediated isothermal amplification and lateral flow assay for various analytes. The method does not require DNA extraction and prevents false positive results. The whole detection process can be completed within 25 minutes, and the accuracy is measured using the polymorphisms of methylenetetrahydrofolate reductase.
Review
Chemistry, Multidisciplinary
Marcelino Varona, Jared L. Anderson
Summary: Detection of mutations and single-nucleotide polymorphisms is crucial for diagnostic applications, and Loop-mediated isothermal amplification (LAMP) is a powerful tool for this purpose. However, traditional LAMP lacks the ability to distinguish single-nucleotide differences within target sequences. Various approaches have been developed to address this limitation, including primer design modifications and probe-based detection methods.
Article
Food Science & Technology
Luis A. Tortajada-Genaro, Maria Isabel Lucio, Angel Maquieira
Summary: This article introduces an instrument-free method for food detection, which is based on detecting genes related to food allergies and fraudulent practices. The method utilizes tailed recombinase polymerase amplification and gold nanoparticles for amplification and optical detection of target regions. The method has advantages such as short amplification time, simplicity, and low sample and reagent volumes, making it suitable for implementation in the food industry.
Article
Chemistry, Analytical
Mengyun Zhou, Chuan Fan, Lirong Wang, Tailin Xu, Xueji Zhang
Summary: This study reports an integrated microdroplet array platform composed of an ultrasonic unit and minipillar array for enhanced RPA detection of SARS-CoV-2. The independent microvolume reactions and microstreaming driven by ultrasound greatly accelerate the amplification and increase the sensitivity, while reducing reagent consumption.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Wenjun Jiang, Rashid Aman, Zahir Ali, Gundra S. Rao, Magdy Mahfouz
Summary: This study presents a new peptide nucleic acid (PNA)-based nucleic acid detection toolkit, PNA-Pdx, which provides accurate and rapid detection of specific nucleic acid sequences, including pathogens and genomic sequences, such as SARS-CoV-2, in clinical samples.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wenjun Jiang, Rashid Aman, Zahir Ali, Gundra S. Rao, Magdy Mahfouz
Summary: PNA-Pdx is a novel peptide nucleic acid (PNA)-based nucleic acid detection toolkit that can accurately and rapidly detect specific nucleic acid sequences. It can also invade double-stranded DNA to produce single-stranded DNA for precise detection of genomic SNPs.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Chao Zhang, Tarun Belwal, Zisheng Luo, Bin Su, Xingyu Lin
Summary: This article comprehensively discusses the application of different nanomaterials in isothermal nucleic acid amplification, including the roles of 0D, 1D, 2D, and 3D nanomaterials, as well as recent progress in the field. Nanomaterials are commonly used for reaction enhancement, signal generation/amplification, or surface loading. Additionally, 3D nanomaterials can serve as isolated chambers for digital nucleic acid amplification and DNA sequencing tools, while challenges and recommendations are proposed for future applications.
Article
Chemistry, Analytical
Ana Lazaro, Eric Seiti Yamanaka, Angel Maquieira, Luis A. Tortajada-Genaro
SENSORS AND ACTUATORS B-CHEMICAL
(2019)
Review
Chemistry, Analytical
Sergi Morais, Luis A. Tortajada-Genaro, Angel Maquieira, Miguel-Angel Gonzalez Martinez
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2020)
Article
Environmental Sciences
Esther Borras, Luis Antonio Tortajada-Genaro, Francisco Sanz, Amalia Munoz
Summary: The study investigated the chemical characteristics of organic aerosols in the western Mediterranean, finding that the organic PM fraction mainly originates from biogenic pollutants emitted directly by vegetation, as well as degradation products of anthropogenic and biogenic volatile organic pollutants. Additionally, carboxylic acids from anthropogenic sources-mainly traffic and cooking-were identified. The region is of interest for aerosol studies due to various processes such as local coastal breezes, industrial emissions, and desert intrusions that affect fine PM composition.
Article
Biochemical Research Methods
Ana Lazaro, Luis A. Tortajada-Genaro, Angel Maquieira
Summary: An accurate genetic diagnostic is crucial for patient management and healthcare systems. The development of methods to discriminate patients with genetic variations present in tumor cells at low concentrations poses a scientific challenge. EAB-qPCR is a promising method that can accurately detect genetic mutations with high sensitivity and reproducibility, making it suitable for routine clinical use.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Luis Antonio Tortajada-Genaro, Angel Maquieira
Summary: The development of a method combining RPA and hybridization analysis allows for rapid and accurate detection of transgenic ingredients in food, supporting compliance with product labeling and traceability regulations. The optical sensing of RPA products using compact disc technology showed excellent selectivity, reproducibility, and sensitivity. This isothermal method provides an effective high-throughput tool for simple and low-cost food safety controls, ideal for laboratories with limited resources.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Chemistry, Multidisciplinary
Ana Lazaro, Angel Maquieira, Luis A. Tortajada-Genaro
Summary: This study presents a multiplexed and highly sensitive approach based on allele-specific hybridization chain reaction (AS-HCR) for detecting single-nucleotide variants. The developed method is rapid, simple, accurate, and cost-effective, making it suitable for detecting clinically relevant genetic variants.
Article
Food Science & Technology
Luis A. Tortajada-Genaro, Maria Isabel Lucio, Angel Maquieira
Summary: This article introduces an instrument-free method for food detection, which is based on detecting genes related to food allergies and fraudulent practices. The method utilizes tailed recombinase polymerase amplification and gold nanoparticles for amplification and optical detection of target regions. The method has advantages such as short amplification time, simplicity, and low sample and reagent volumes, making it suitable for implementation in the food industry.
Article
Chemistry, Analytical
Luis A. Tortajada-Genaro, Natalia Casan-Raga, Salva Mas, Ethel Ibanez-Echevarria, Sergi Morais, Angel Maquieira
Summary: This study presents an in vitro biosensing method for specific IgE levels based on a reversed-phase allergen array. It combines optical biosensing, direct multiplex immunoassays, and on-disc technology to identify 12 sIgE associated with food allergies in a single analysis with a low serum sample volume. The method has excellent clinical specificity (100%) and high sensitivity (91.1%), making it potentially useful for diagnosing multiple food allergies.
Article
Chemistry, Analytical
Luis A. Tortajada-Genaro, Ana Lazaro, Sara Martorell, Angel Maquieira
Summary: In the context of personalized and cost-effective treatment, knowing the mutation status of specific genes can help predict which patients will respond to therapies. The presented genotyping tool uses a colorimetric DNA array to detect multiple polymorphic sequences with a single nucleotide variation, providing an alternative to one-by-one detection or massive sequencing. The developed technology, which includes sequence-tailored probes and PCR with SuperSelective primers, offers a fast, cheap, and reproducible method for discriminating oncological patients.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Sara Martorell, Angel Maquieira, Luis A. Tortajada-Genaro
Summary: A biosensing system was developed for accurately detecting single nucleotide changes in the target organism genome. This system integrates selective isothermal amplification and a sensitive dendron-mediated DNA hybridization assay. The use of dendrons as crosslinkers and the preparation of allele-specific probes using click-chemistry allowed for the creation of forest-array chips compatible with current microarray fabrication technologies. The developed approach exhibited sensitive recognition of DNA variants and was successfully applied to detect a specific mutation in the PIK3CA gene from clinical samples.
Article
Education & Educational Research
Borja Vidal, M. Loreto Fenollosa Ribera, Francisco Javier Ribal, Pablo Sanchis, Jaime Garcia-Ruperez, M. Amparo Bes-Pia, E. Blasco-Tamarit, Patricia Noguera, Maria Jose Munoz-Portero, Luis A. Tortajada
Summary: This study explores students' preferences for learning methods in seven engineering disciplines at a Spanish technical university, finding that project-based learning and problem-based learning are perceived as more effective. Results also suggest that students can be classified into two groups based on their level of activeness in learning.
JOURNAL OF APPLIED RESEARCH IN HIGHER EDUCATION
(2022)
Article
Meteorology & Atmospheric Sciences
Esther Borras, Luis A. Tortajada-Genaro, Milagro Rodenas, Teresa Vera, Thomas Speak, Paul Seakins, Marvin D. Shaw, Alastair C. Lewis, Amalia Munoz
Summary: The study developed a technique for near-real-time monitoring of multi-oxygenated compounds in the atmosphere, offering advantages in terms of handling and labor time for quantifying trace levels of these compounds accurately. The method was successfully applied in air samples and atmospheric experiments, demonstrating its capability and accuracy for atmospheric monitoring.
ATMOSPHERIC MEASUREMENT TECHNIQUES
(2021)
Article
Chemistry, Analytical
Luis A. Tortajada-Genaro, Regina Ninoles, Salvador Mena, Angel Maquieira
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).