Article
Chemistry, Multidisciplinary
Gokul Chandra Biswas, Hiroaki Suzuki
Summary: We have introduced a simple-to-use manual roller pump-driven and valve-free microfluidic system for sequential solution exchange and protein detection. The system is characterized by its simplicity, quick operation, and suitability for emergencies and situations with limited resources.
Review
Biotechnology & Applied Microbiology
Kaimin Wu, Xuliang He, Jinglei Wang, Ting Pan, Ran He, Feizhi Kong, Zhenmin Cao, Feiye Ju, Zhao Huang, Libo Nie
Summary: Microfluidic chip technology is a technology platform that integrates basic operation units such as processing, separation, reaction, and detection into a microchannel chip to achieve low consumption, fast and efficient sample analysis. It has the characteristics of small sample and reagent volume, fast analysis, low cost, automation, portability, high throughput, and good compatibility with other techniques. The future development of microfluidic chips lies in point-of-care testing and high throughput equipment, but there are still challenges in the design and integration of microfluidic chips, as well as the analysis of actual samples.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biophysics
Xinran Xiang, Qinghua Ye, Yuting Shang, Fan Li, Baoqing Zhou, Yanna Shao, Chufang Wang, Jumei Zhang, Liang Xue, Moutong Chen, Yu Ding, Qingping Wu
Summary: A novel microfluidic chip was developed for rapid and sensitive detection of AFB1, showing a wide analytical range and low detection limit, as well as good specificity and reusability. The chip design provides a reliable scheme for on-site detection of AFB1 and analysis of other small molecules.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Applied
Mantong Zhao, Xiaolong Li, Yule Zhang, Yuwen Wang, Bo Wang, Lulu Zheng, Dawei Zhang, Songlin Zhuang
Summary: An automated microfluidics system was developed for the detection of chloramphenicol (CAP) residues in milk, which had high reliability, low cost, a good detection limit, and recovery rate. This system showed significant potential for point-of-care testing (POCT) of CAP in milk.
Article
Chemistry, Analytical
Janosch Hauser, Matilda Dale, Olof Beck, Jochen M. Schwenk, Claudia Fredolini, Goeran Stemme, Niclas Roxhed
Summary: Patient-centric sampling strategies involving self sampling and centralized laboratory readout are becoming widely adopted. A capillary-driven microfluidic device is presented here for performing biomarker capturing during sample collection, enabling biohazard-free shipment and utilization of advanced laboratory analytics. The device demonstrates multiplexing capability and applicability to patient-centric workflows using various blood biomarkers. The quantification of a biomarker panel offers new possibilities for e-doctor and e-health applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ke Ning, Huihui Chai, Yanxia Cui, Shiming Wu, Feng Chen, Yuan Li, Ping Qiu, Wei Sun, Ling Yu
Summary: A centrifugal microfluidic chip was designed to test blood beta(2) microglobulins (beta(2) M) levels, with a handhold centrifuge used for solution mixing. An office scanner recorded the reaction results, reducing reliance on expensive scientific equipment. The chip-based test showed a sensitivity of 77.3%, specificity of 82.1%, and efficiency of 80%.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Wei Wang, Xiaoyu Cai, Qingling Li, Lili Zheng, Xiufeng Yu, Huimin Zhang, Jian Wang
Summary: This study developed an immunoassay method based on a paper chip, which can detect AFP quickly and cost-effectively by immobilizing antibodies on the chip surface. The method showed reliable performance in diagnosing liver diseases by detecting AFP levels in serum samples of normal individuals and patients with liver conditions.
Article
Engineering, Multidisciplinary
Tong Hou, Hui Chang, Haoliang Jiang, Pengcheng Wang, Na Li, Yongxin Song, Dongqing Li
Summary: The smartphone integrated microfluidic lab-on-chip device enables real-time detection, counting, and sizing of living algae in ship's ballast water, achieving high accuracy and convenience in operation.
Article
Chemistry, Analytical
Amir Osman Abdelrazig, Bao Thai Tran, Patsamon Rijiravanich, Werasak Surareungchai
Summary: A microfluidic analytical device based on wax-patterned Fusion 5 paper was developed for rapid nucleic acid detection of the main causal agent of chili anthracnose in Asia. The biosensor used DNA-conjugated gold nanoparticles for detection with a detection limit down to 17.42 femtomoles, offering a specific and on-site detection method within 15 minutes. The device shows potential for protein assays and has a significant impact on on-site diagnostics.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Multidisciplinary
Weiqi Zhao, Yang Zhou, Yao-Ze Feng, Xiaohu Niu, Yongkun Zhao, Junpeng Zhao, Yongzhen Dong, Mingqian Tan, Yunlei Xianyu, Yiping Chen
Summary: In this work, a multidimensional digital immunoassay has been developed using microparticle-based encoding and artificial intelligence-based decoding, enabling multiplexed detection with high sensitivity and convenient operation. The features of microspheres, including size, number, and color, encode information for simultaneous identification and accurate quantification of multiple targets. Computer vision-based artificial intelligence analyzes microscopy images for information decoding and visually outputs identification results. Additionally, optical microscopy imaging can be integrated with microfluidic platforms for encoding-decoding using computer vision-based artificial intelligence. This microfluidic digital immunoassay can simultaneously analyze multiple inflammatory markers and antibiotics within 30 minutes, with a broad detection range from pg/mL to μg/mL, holding great promise as an intelligent bioassay for next-generation multiplexed biosensing.
Article
Biochemical Research Methods
Jiadi Sun, Yijing Ren, Jian Ji, Yu Guo, Xiulan Sun
Summary: A novel concentration gradient microfluidic chip was designed for sensitive and rapid antibiotic susceptibility testing. Experimental results confirmed the linearity of the concentration gradient, explored optimal operating parameters, and studied the impact of acid-based food processing. The method proved to be highly effective for determining bacterial antibiotic resistance.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Shiuan-Haur Su, Yujing Song, Michael W. Newstead, Tao Cai, MengXi Wu, Andrew Stephens, Benjamin H. Singer, Katsuo Kurabayashi
Summary: The digital Phenoplate (dPP) is an integrated platform that allows ultra-sensitive measurement of cellular cytokine secretory profiles, making it suitable for high precision and high throughput characterization of cellular phenotypes.
Article
Chemistry, Analytical
Asma Dhehibi, Abdelmounaaim Allaoui, Amal Raouafi, Mohammed Terrak, Balkiss Bouhaouala-Zahar, Mohamed Hammadi, Noureddine Raouafi, Imed Salhi
Summary: In this study, a nanobody-based magnetofluorescent immunoassay was designed for the sensitive detection of pathogenic E. coli F17-positive strains. The assay recognized E. coli F17 with high specificity and sensitivity, with a detection limit of 1.8 CFU/mL in fecal samples without pretreatment. The immunoassay remained stable for at least one month when stored at 4 degrees C.
Review
Medicine, Research & Experimental
Trey W. Pittman, Daniel Balazs Decsi, Chamindie Punyadeera, Charles S. Henry
Summary: There has been a growing interest in using microfluidic devices for saliva analysis in recent years. This approach has the potential to provide more sensitive and selective analysis by collecting large quantities of saliva samples. The aim of this review is to provide an update on the use of microfluidic devices for salivary biomarker analysis.
Article
Chemistry, Analytical
Xiuxiu Li, Chenglong Xu, Hao Chen, Fei Yi, Jiaqi Liao, Jianmei Han, Chonghao Li, Weihua Han, Ray P. S. Han, Hong Chen
Summary: Point-of-care testing methods currently utilize rapid, portable, inexpensive, and multiplexed on-site detection. Microfluidic chips have become a very promising platform with broad development prospects due to their breakthrough improvement in miniaturization and integration. However, the conventional microfluidic chips still have disadvantages, such as difficulty in fabrication processing, long production time and high cost, which hinder its applications in the fields of POCT and in vitro diagnostics. In this study, a capillary-based microfluidic chip with the characteristics of low cost and easy fabrication was developed for the rapid detection of acute myocardial infarction (AMI).
Article
Chemistry, Multidisciplinary
Qin Xu, Yongchun Pan, Xinli Liu, Yanfeng Gao, Xiaowei Luan, Fei Zeng, Dongtao Zhou, Wenxiu Long, Yuzhen Wang, Yujun Song
Summary: This study reports a novel nanosensor that can provide early cancer monitoring through urinary detection under tumor-specific hypoxia conditions. The nanosensor can be quantified using a companion volumetric bar-chart chip and can also monitor the efficacy of treatment.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Chemistry, Analytical
Yanping Wang, Yanfeng Gao, Yi Yin, Yongchun Pan, Yuzhen Wang, Yujun Song
Summary: The simultaneous detection of different biomarkers in a single test using microfluidic devices assisted by nanomaterials has significant implications for accurate disease diagnosis and therapy monitoring. These integrated platforms offer advantages such as minimal sample consumption, high sensitivity, low detection limit, enhanced signals, and reduced detection time, making them highly promising for point-of-care testing and clinical diagnostics.
Article
Biophysics
Yongchun Pan, Xiaowei Luan, Fei Zeng, Qin Xu, Zekun Li, Yanfeng Gao, Xinli Liu, Xueqing Li, Xin Han, Jianliang Shen, Yujun Song
Summary: This study develops a CRISPR/aptamer-based sensor, sheltered by a hollow COF, for ATP imaging in living animals. This strategy not only expands the applications of the CRISPR-Cas system, but also provides potential for the use of other proteins in clinical diagnostics, medical research, and biomimetic nanodevices.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Pharmacology & Pharmacy
Zekun Li, Yongchun Pan, Shiyu Du, Yayao Li, Chao Chen, Hongxiu Song, Yueyao Wu, Xiaowei Luan, Qin Xu, Xiaoxiang Guan, Yujun Song, Xin Han
Summary: NIR-light-triggered nanomedicine, including PDT and PTT, is an attractive approach for cancer therapy due to its controllability and minimal invasion. A tumor-microenvironment responsive CRISPR nanoplatform based on oxygen-deficient titania has been developed for mild NIR-phototherapy. In the tumor microenvironment, the overexpressed HAase and GSH can destroy HA and disulfide bond, releasing Cas9/sgRNA to target stress alleviating regulators and reduce stress tolerance of tumor cells. Under NIR light, the TiO2 exhibits a higher anticancer effect.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Chemistry, Multidisciplinary
Dongtao Zhou, Yi Yin, Zhenxing Zhu, Yanfeng Gao, Jingjing Yang, Yongchun Pan, Yujun Song
Summary: Inflammatory bowel disease (IBD) is a chronic autoimmune disease with increasing incidence globally. This study presents an orally administered nanosensor that releases ultrasmall platinum nanoclusters (PtNCs) in IBD-related inflammatory environments, enabling non-invasive monitoring and diagnosis.
Article
Chemistry, Multidisciplinary
Xiaowei Luan, Yongchun Pan, Yuyu Zhou, Dongtao Zhou, Wenjian Zhao, Fei Zeng, Zhenxing Zhu, Qiangbing Lu, Qianglan Lu, Yanfeng Gao, Guanzhong He, Minghui Lu, Yujun Song
Summary: This study developed a pH-responsive reversible self-assembled Cu2-xSe-BSA nanodrug that can be renal cleared and selectively targeted aggregated in cancer lysosomes, enhancing tumor accumulation and retention. Furthermore, the nanodrug also demonstrated enhanced photoacoustic imaging signal, photothermal therapy, and chemodynamic therapy capability.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Chemistry, Medicinal
Yanping Wang, Yanfeng Gao, Yujun Song
Summary: Urine biopsy, as an emerging liquid biopsy strategy, offers advantages such as non-invasiveness, large sample volume, and simple sampling operation. Microfluidics, with its miniaturization, low cost, high integration, high throughput, and low sample consumption, has shown outstanding performance in urine biopsy for cancer detection.
Article
Chemistry, Multidisciplinary
Shiyu Du, Chao Chen, Suchen Qu, Hongxiu Song, Jingjing Yang, Yayao Li, Kunguo Liu, Qianglan Lu, Wen Luo, Runtian Wang, Xiaoxiang Guan, Yujun Song, Xin Han
Summary: Immunotherapy is a promising therapeutic strategy against cancer, but it faces challenges. This study develops a strategy of multiple activation of immune pathways to treat tumors through the combination of gene therapy and immunotherapy. The study finds that a pH/glutathione responsive drug-loading nanosystem can effectively enhance drug delivery and generate immune activation effects to destroy tumor cells.
Article
Chemistry, Multidisciplinary
Yayao Li, Yongchun Pan, Chao Chen, Zekun Li, Shiyu Du, Xiaowei Luan, Yanfeng Gao, Xin Han, Yujun Song
Summary: In this study, a pH/glutathione (GSH) dual stimuli-responsive CRISPR/Cas9 gene-editing nanoplatform combined with calcium-enhanced CO gas therapy was established for precise anticancer therapy. The platform demonstrated the ability to regulate the tumor microenvironment, perform gene ablation, and interfere with the ROS signaling pathway, showing great potential for future clinical applications in precise nanomedicine.
Article
Chemistry, Analytical
Yanfeng Gao, Yanping Wang, Xinli Liu, Zhenxing Zhu, Zhun Li, Zhibin Zhang, Yi Yin, William Chi Shing Cho, Yujun Song, Yuzhen Wang
Summary: We developed a signal amplification method based on streamlined click reactions to immobilize large amounts of hemin on graphene oxide nanosheets, allowing for highly sensitive detection of various biological targets. The designed platform shows great promise for graphene-based nanozymes and sensitive biosensing in a wider range of applications.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Wenxiu Long, Jingjing Yang, Qiao Zhao, Yongchun Pan, Xiaowei Luan, Bangshun He, Xin Han, Yuzhen Wang, Yujun Song
Summary: This study reports the integration of a metal-organic framework (MOF)-based DNA bio-barcode with the CRISPR/Cas12a system for ultrasensitive detection of protein biomarkers. The MOF-DNA bio-barcode strategy amplified the CRISPR/Cas12a system to achieve simple and highly sensitive detection of tumor protein biomarkers. This platform offers a powerful tool for early disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Biomedical
Qianglan Lu, Ruiyue Chen, Shiyu Du, Chao Chen, Yongchun Pan, Xiaowei Luan, Jingjing Yang, Fei Zeng, Bangshun He, Xin Han, Yujun Song
Summary: This study reports a nanoplatform that utilizes CRISPR-Cas9 gene editing and cGAS-STING pathway activation to trigger long-term immunotherapy by silencing PD-L1 immune checkpoint. In the tumor microenvironment, the nanoplatform can persistently activate the cGAS-STING pathway and release large amounts of interferon and pro-inflammatory factors. Additionally, the platform can use CRISPR-Cas9 technology to silence the PD-L1 gene, reactivating immune cells to fight against tumors.
Article
Chemistry, Multidisciplinary
Qiao Zhao, Bei Pan, Wenxiu Long, Yongchun Pan, Dongtao Zhou, Xiaowei Luan, Bangshun He, Yuzhen Wang, Yujun Song
Summary: By integrating metal-organic frameworks (MOFs) based biobarcodes with CRISPR/Cas12a assay, the study achieved sensitive detection of microRNAs, presenting a universal strategy for miRNA detection with high sensitivity.
Review
Chemistry, Analytical
Yanfeng Gao, Yanping Wang, Yuzhen Wang, Pascale Magaud, Yuta Liu, Fei Zeng, Jingjing Yang, Lucien Baldas, Yujun Song
Summary: Accurate, sensitive, and highly selective detection of biological targets is crucial in various fields. The combination of nanocatalysis and microfluidics has shown great success in bioanalysis and offers numerous applications for different biological targets. This review summarizes the recent developments in nanocatalysis-assisted bioanalytical microfluidic devices, including various catalytically active nanomaterials, microfluidic approaches, detection techniques, and their applications. The integration of nanocatalysts for signal conversion and amplification is emphasized to enhance detection sensitivity. Furthermore, the challenges and future trends in nanocatalysis-assisted microfluidics are discussed, aiming to guide the development of practical point-of-care testing devices.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yuanyue Han, Xinli Liu, Qiao Zhao, Yanfeng Gao, Dongtao Zhou, Wenxiu Long, Yuzhen Wang, Yujun Song
Summary: A simple aptazyme-induced cascade signal amplification (ACSA) was integrated with a triple-channel volumetric bar-chart chip (TV-Chip) for the visual quantification of aflatoxin B1 (AFB1) and adenosine triphosphate (ATP). Bifunctional aptazymes modified on magnetic silica nanoparticles (MSNPs) were used as sensing probes. The detection limits of AFB1 and ATP were easily achieved without additional instruments. The results were in good agreement with a commercial AFB1 ELISA kit, showing the accuracy and reliability of this method. The ACSA-based TV-Chip holds great promise for on-site and real-time quantitation of trace targets.
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
