Journal
REVISTA ARGENTINA DE MICROBIOLOGIA
Volume 52, Issue 1, Pages 4-12Publisher
ASOCIACION ARGENTINA MICROBIOLOGIA
DOI: 10.1016/j.ram.2019.02.005
Keywords
Cellulolytic enzymes; Penicillium funiculosum; Fusarium verticillioides; Cladosporium cladosporioides; Moringa oleifera
Categories
Funding
- Secretaria de Investigacion y Posgrado del Instituto Politecnico Nacional (SIP-IPN) [20131491, 20144369, 20150270, 20160483]
- CONACYT, Mexico [2011-16-175519, 270245, 291143]
Ask authors/readers for more resources
Currently, the valorization of agroindustrial waste is of great interest. Moringa oleifera is a multipurpose tree whose softwood residues could be used as raw material for low-cost cellulase production. The aim of this study was to isolate, identify, and characterize microorganisms with cellulolytic activity in different carbon sources. We isolated and purified 42 microorganisms from M. oleifera biomass. Fungi presenting the largest hydrolytic halos in carboxymethylcellulose as a substrate were molecularly identified as Penicillium funiculosum (FG1), Fusarium verticillioides (FG3) and Cladosporium cladosporioides (FC2). The ability of these fungal strains to break down cellulose was assessed in a submerged fermentation using either amorphous CMC or crystalline form (Avicel). P. funiculosum and C. cladosporioides displayed similar endoglucanase (606 U/l) and exoglucanase (205 U/l) activities in the Avicel-containing medium, whereas F. verticillioides showed the highest level of beta-glucosidase activity (664 U/l) in the carboxymethylcellulose medium. In addition, the effect of three culture media (A, B, and C) on cellulase production was evaluated in P. funiculosum using moringa straw as a carbon source. The results showed a volumetric productivity improvement of cellulases that was 2.77-, 8.26-, and 2.30-fold higher for endoglucanase, exoglucanase and beta-glucosidase, respectively when medium C containing moringa straw was used as a carbon source. The enzymatic extracts produced by these fungi have biotechnological potential especially for second-generation bioethanol production (2G) from moringa straw. This is the first report on the use of M. oleifera biomass to induce the production of various cellulases in P. funiculosum. (C) 2019 Asociacion Argentina de Microbiologia. Published by Elsevier Espana, S.L.U.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available