Article
Agriculture, Dairy & Animal Science
Ali Daneshmand, Hassan Kermanshahi, Javid Mohammed, Mohammad Hadi Sekhavati, Ali Javadmanesh, Monireh Ahmadian, Marzieh Alizadeh, Jamshid Razmyar, Raveendra R. Kulkarni
Summary: This study evaluated the effects of C. perfringens on the intestines, microbial colonization, and immune responses in broiler chickens. The results showed that C. perfringens can cause intestinal damage, changes in microbial populations, and trigger host immune responses. Furthermore, using coccidia and a wheat-based diet predisposition may facilitate better research on C. perfringens.
Article
Agriculture, Dairy & Animal Science
Shifeng Wang, Charles L. Hofacre, Soo-Young Wanda, Jingyu Zhou, Richard A. Callum, Bob Nordgren, Roy Curtiss
Summary: We have improved a vaccine for C. perfringens by integrating a triple-sugar regulation system and a DsifA mutation, which resulted in increased protection and a safer profile.
Article
Immunology
Jin Liu, Shuangqi Wu, Honghao Zhao, Chun Ye, Shulin Fu, Yu Liu, Ting Liu, Yinsheng Qiu
Summary: Baicalin-aluminum complex, synthesized from baicalin, inhibits the virulence-associated traits and virulence genes expression of Clostridium perfringens. It has potential as a candidate against C. perfringens infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Kosar Gharib-Naseri, Sarbast Kheravii, Chake Keerqin, Robert A. Swick, Mingan Choct, Shu-Biao Wu
Summary: The primary cause of necrotic enteritis in chickens is the NetB-positive Clostridium perfringens bacterium. This study evaluated the impact of two pathogenic C. perfringens strains on gut health and gene expression in broilers, finding that the challenge is detrimental to performance and different strains can modulate the severity of the disease through gene expression.
Article
Agriculture, Dairy & Animal Science
Dorthe Sandvang, Line Skjoet-Rasmussen, Mette Dines Cantor, Greg F. Mathis, Brett S. Lumpkins, Alfred Blanch
Summary: The study evaluated the probiotic effects of 3 different Bacillus strains and their combination on broiler chickens through in vitro and in vivo experiments. The results showed that the multi-strain combination significantly improved the performance and reduced the necrotic enteritis lesion scores of the chickens, as well as decreased the mortality rate caused by necrotic enteritis.
Article
Microbiology
Martina Husta, Svitlana Tretiak, Richard Ducatelle, Filip Van Immerseel, Evy Goossens
Summary: This study evaluated the behavior of C. perfringens in different intestinal compartments of broiler chickens during a NE trial. The results showed that necrotic enteritis-associated lesions were mainly found in the jejunum and were more severe compared to the duodenum and ileum. There was a positive correlation between the total number of vegetative C. perfringens cells in the intestinal compartments and disease severity. C. perfringens was mainly present as spores in the caecum and distal colon.
VETERINARY MICROBIOLOGY
(2023)
Review
Microbiology
Shahna Fathima, Walid Ghazi Al Hakeem, Revathi Shanmugasundaram, Ramesh K. Selvaraj
Summary: Clostridium perfringens type A and C are the primary etiological agents associated with necrotic enteritis (NE) in poultry, with virulence factors including alpha-toxin, beta-toxin, and NetB toxin. The control strategies for NE mainly focus on positive modulation of host immune response, nutritional manipulation, and pathogen reduction.
Article
Microbiology
Carissa Gaghan, Kaitlin Gorrell, Khaled Taha-Abdelaziz, Shayan Sharif, Raveendra R. Kulkarni
Summary: Necrotic enteritis (NE) is a significant disease of chickens, causing economic losses. In this study, chickens were inoculated intracloacally with different strains of C. perfringens to evaluate the severity of NE and immune responses. The results showed that chickens infected with CP18 and CP26 had reduced weight gain and milder NE lesions. Gene expression analysis revealed increased expression of anti-inflammatory/immunoregulatory factors in certain lymphoid organs, suggesting a highly regulated immune response.
Article
Agriculture, Dairy & Animal Science
Xieying Ding, Xin Zhong, Yunqiao Yang, Geyin Zhang, Hongbin Si
Summary: This study confirmed the combined inhibitory effect of citric acid (CA) and magnolol (MA) on C. perfringens. The optimal ratio of CA to MA was found to be 50:3, with a dosage of 265 μg/mL inhibiting the growth of C. perfringens and causing damage to its cell structure. The combination of CA and MA exhibited therapeutic effects on C. perfringens challenge in chickens by inhibiting inflammation and enhancing antioxidant capability. Rating: 8/10.
Article
Biotechnology & Applied Microbiology
Ilhem Meniai, Alexandre Thibodeau, Sylvain Quessy, Valeria R. Parreira, Philippe Fravalo, Guy Beauchamp, Marie-Lou Gaucher
Summary: The study identified potential immunogenic proteins of C. perfringens strains through genomic analysis, resulting in the selection of 12 candidate proteins, with 6 showing higher antigenic potential and possible association with the virulence of C. perfringens.
Article
Agriculture, Dairy & Animal Science
Larissa Justino, Ana A. S. Baptista, Marielen de Souza, Maisa F. Menck-Costa, Barbara G. Pires, Claudineia E. Cicero, Ana P. F. R. L. Bracarense, Vanessa M. Kaneko, Alexandre Oba, Adriano S. Okamoto, Raphael L. Andreatti Filho
Summary: The ban of antibiotic growth promoters in animal feed has increased the number of cases of necrotic enteritis (NE) in broilers, which greatly impacts the poultry industry. However, inducing NE in experimental settings is challenging due to its multifactorial nature. This study evaluated the effects of immunosuppression, infection, or both, by Eimeria spp., on the predisposition to NE in broilers fed a wheat-based diet and challenged with different strains of Clostridium perfringens. The results suggest a synergistic effect between the strain of C. perfringens and the predisposing factors, but further evaluation is needed.
Article
Veterinary Sciences
Chake Keerqin, Katherine McGlashan, Thi Thu Hao Van, Helene N. Chinivasagam, Robert J. Moore, Mingan Choct, Shu-Biao Wu
Summary: This study isolated three highly active bacteriophages from Australian commercial poultry environments, which were capable of lysing several disease-causing bacteria. Animal trials showed that oral administration of the bacteriophages cocktail significantly alleviated intestinal necrotic lesions. However, no effect on growth performance was observed.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
A. H. Moritz, B. Lumpkins, G. F. Mathis, W. C. Bridges, S. Wilson, M. E. Blair, R. E. Buresh, J. R. Strickland, M. Arguelles-Ramos
Summary: A 28-day battery cage study conducted on male broiler chickens examined the effects of tannin-free grain sorghum varieties on gut health and growth efficiency when challenged with Eimeria maxima and Clostridium perfringens. The study found that birds fed white/tan sorghum showed higher body weight gain compared to those fed red/bronze sorghum. Additionally, birds fed red/bronze and white/tan sorghum had reduced intestinal lesions compared to those fed U.S. No. 2 sorghum and corn. This study demonstrates the potential benefits of select grain sorghum varieties in improving gut health and growth efficiency in broilers under challenge conditions.
Article
Biotechnology & Applied Microbiology
Mohammad Ali Shamshirgaran, Mehdi Golchin, Elham Mohammadi
Summary: In this study, a recombinant Lactobacillus casei strain expressing the NetB protein of Clostridium perfringens was used as a probiotic-based vector vaccine to immunize broiler chickens against necrotic enteritis. The vaccinated birds showed significant protection against the disease and developed strong antibody responses. This study suggests that a probiotic-based vector vaccine could be a promising candidate for controlling necrotic enteritis in broiler chickens.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
D. Goo, U. D. Gadde, W. K. Kim, C. G. Gay, E. W. Porta, S. W. Jones, S. Walker, H. S. Lillehoj
Summary: This study presents a passive immunization technology using hyperimmune egg yolk immunoglobulin Y (IgY) to protect against Necrotic enteritis (NE) caused by Clostridium perfringens in poultry. The protective effects of the IgYs were evaluated in commercial broilers, and it was found that the dietary mixture containing antibodies provided protection against NE through passive immunization.
Article
Agriculture, Dairy & Animal Science
Johanna O. Zeitz, Sarah Mohrmann, Stella C. Kaeding, Murat Devlikamov, Ines Niewalda, Rose Whelan, Ariane Helmbrecht, Klaus Eder
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2019)
Article
Agriculture, Dairy & Animal Science
Rose A. Whelan, Kiran Doranalli, Teemu Rinttila, Kirsi Vienola, German Jurgens, Juha Apajalahti
Article
Agriculture, Dairy & Animal Science
M. S. Abdul Rasheed, M. L. Oelschlager, B. N. Smith, L. L. Bauer, R. A. Whelan, R. N. Dilger
Article
Agriculture, Dairy & Animal Science
Zhouzheng Ren, Daniel E. Butz, Rose Whelan, Victor Naranjo, Maria K. Arendt, Mitchell D. Ramuta, Xiaojun Yang, Thomas D. Crenshaw, Mark E. Cook
Article
Agriculture, Dairy & Animal Science
Guanchen Liu, Andrew D. Magnuson, Tao Sun, Samar A. Tolba, Charles Starkey, Rose Whelan, Xin Gen Lei
JOURNAL OF ANIMAL SCIENCE
(2019)
Article
Agriculture, Dairy & Animal Science
M. S. Abdul Rasheed, M. L. Oelschlager, B. N. Smith, L. L. Bauer, R. A. Whelan, R. N. Dilger
Article
Agriculture, Dairy & Animal Science
Andrew D. Magnuson, Guanchen Liu, Tao Sun, Samar A. Tolba, Lin Xi, Rose Whelan, Xin Gen Lei
JOURNAL OF ANIMAL SCIENCE
(2020)
Article
Nutrition & Dietetics
Stella Romanet, Joerg R. Aschenbach, Robert Pieper, Juergen Zentek, John K. Htoo, Rose A. Whelan, Lucia Mastrototaro
JOURNAL OF NUTRITION
(2020)
Article
Veterinary Sciences
Mohammad Kamely, Wanwei He, Jeremy Wakaruk, Rose Whelan, Victor Naranjo, Daniel R. Barreda
FRONTIERS IN VETERINARY SCIENCE
(2020)
Article
Agriculture, Dairy & Animal Science
Johanna O. Zeitz, Anne Fleischmann, Tamara Ehbrecht, Erika Most, Silvia Friedrichs, Rose Whelan, Denise K. Gessner, Klaus Failing, Dieter Luetjohann, Klaus Eder
Article
Agriculture, Dairy & Animal Science
Yijie He, Cynthia Jinno, Kwangwook Kim, Zhaohai Wu, Bie Tan, Xunde Li, Rose Whelan, Yanhong Liu
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2020)
Article
Biology
Gunter Raddatz, Ryan J. Arsenault, Bridget Aylward, Rose Whelan, Florian Boehl, Frank Lyko
Summary: The study analyzed the DNA methylation machinery and landscape in chickens, identifying hypomethylated sperm DNA and low-methylated regions as conserved gene regulatory elements with tissue-specific methylation patterns. A DNA methylation clock was developed using whole-genome bisulfite sequencing to predict broiler chicken age, and it showed epigenetic age acceleration in animals with experimentally induced inflammation. This research provides insights into the chicken methylome and suggests a novel application of the DNA methylation clock as a marker for livestock health.
COMMUNICATIONS BIOLOGY
(2021)
Article
Genetics & Heredity
Stella Romanet, Joerg R. Aschenbach, Robert Pieper, Juergen Zentek, John K. Htoo, Rose A. Whelan, Lucia Mastrototaro
Summary: This study investigated the effect of different dietary methionine sources on the expression profile of various methionine transporters along the gastrointestinal tract of pigs. The results showed that pigs fed with DL-Met had potential to increase apical methionine transport in the small intestine, whereas pigs fed with DL-HMTBA had potential to increase basolateral methionine transport in the small intestine and other gastrointestinal tissues.
GENES AND NUTRITION
(2021)
Article
Veterinary Sciences
A. Menconi, A. O. Sokale, S. M. Mendoza, R. Whelan, K. Doranalli
Article
Agriculture, Dairy & Animal Science
Johanna O. Zeitz, Tamara Ehbrecht, Anne Fleischmann, Erika Most, Denise K. Gessner, Silvia Friedrichs, Marion Sparenberg, Klaus Failing, Rose Whelan, Dieter Luetjohann, Klaus Eder
Article
Agriculture, Dairy & Animal Science
Jesper Tessin, Judith Rohde, Arne Jung, Nicole Kemper, Jochen Schulz
Summary: In this study, samples from poultry house environment and culled birds were collected, and E. cecorum was successfully isolated. The principle of isolating the pathogen by streaking on a chromogenic agar may motivate researchers to investigate transmission routes and optimize biosecurity measures.
Article
Agriculture, Dairy & Animal Science
Junqin Zhang, Zimin Xie, Yanlin Pan, Zuoxin Chen, Yunzhen Huang, Linlin Li, Jiawen Dong, Yong Xiang, Qi Zhai, Minhua Sun, Xingying Li, Shujian Huang, Ming Liao
Summary: In recent years, there has been an increase in the occurrence of fowl adenovirus 2 (FAdV-2) in China, posing a significant threat to the poultry industry. This study investigated the epidemiology, phylogenetic relationship, genomic characteristics, and pathogenicity of FAdV-2. The results showed that FAdV-2 was the most commonly detected serotype among various FAdV serotypes. Genetic analysis revealed that these FAdV-2 strains formed a distinct branch within the FAdV-D group and had a high nucleotide similarity with reference strains. Recombination analysis identified recombination events between FAdV-2 and FAdV-11 in some strains. Pathogenicity experiments demonstrated that a recombinant FAdV-2 strain caused a high mortality rate and more severe disease in chickens. These findings contribute to our understanding of FAdV-2 and provide a basis for vaccine development.
Article
Agriculture, Dairy & Animal Science
Ebtsam Iraqi, Ali Abdel Hady, Nadia Elsayed, Hanaa Khalil, Amina El-Saadany, Karim El-Sabrout
Summary: Thermal manipulation during egg incubation, particularly at 12 to 18 days, has positive effects on embryonic development, hatching characteristics, chick quality, as well as posthatch thermotolerance and performance.
Article
Agriculture, Dairy & Animal Science
Yong Liu, Mingxin Song, He Bai, Chunhua Wang, Fei Wang, Qi Yuan
Summary: Dietary curcumin supplementation can significantly improve egg quality of quails in the late laying period, primarily by improving lipid metabolism and selectively regulating the intestinal microbial community.
Article
Agriculture, Dairy & Animal Science
Lei Zhang, Zhixuan Song, Peiyao Li, Xingju Song, Xinming Tang, Dandan Hu
Summary: This study evaluated the vaccine potential of recombinant proteins for avian coccidiosis caused by Eimeria. The results showed that the rEten5-B protein can trigger immune protection against E. tenella and may be a potential and effective subunit vaccine for the control of coccidiosis in poultry.
Article
Agriculture, Dairy & Animal Science
Milan Kumar Sharma, Amit Kumar Singh, Doyun Goo, Venkata Sesha Reddy Choppa, Hanseo Ko, Hanyi Shi, Woo Kyun Kim
Summary: The study investigates the effect of Eimeria infection on gastrointestinal physiology, growth performance, and egg production of Hy-Line W-36 laying hens during peak production. Results show that Eimeria infection negatively affects body weight, feed intake, immune response, and nutrient transporter gene expression. Egg production temporarily ceases in hens infected with high and medium-high dosages of Eimeria.
Article
Agriculture, Dairy & Animal Science
Elwy A. Ashour, Mahmoud Kamal, Hayman A. A. Altaie, Ayman A. Swelum, Gamaleldin M. Suliman, Guillermo Tellez-Isaias, Mohamed E. Abd El-Hack
Summary: This study evaluated the effects of CP and ME on the performance and nutritional value of Japanese quail lay chicken. The results showed that protein and energy levels significantly influenced all productive outcomes except feed conversion ratio. The combination of dietary energy and protein levels had a substantial effect on all egg quality trials evaluated. It is recommended to feed layer Japanese quail between the ages of 8 and 20 weeks during the summer with a dietary energy content of 2,900 kcal ME/kg and 20% CP.
Article
Agriculture, Dairy & Animal Science
J. E. Doornweerd, R. F. Veerkamp, B. de Klerk, M. van der Sluis, A. C. Bouwman, E. D. Ellen, G. Kootstra
Summary: Tracking group-housed individual broilers using video can provide valuable information on their health, welfare, and performance. This study conducted a comprehensive analysis on broiler tracking on video and potential tracking errors, highlighting the potential and challenges of phenotyping broiler locomotion. The results emphasized the importance of addressing tracking errors, potential algorithm improvements, and the need for an external animal identification system for efficient locomotion phenotyping.
Article
Agriculture, Dairy & Animal Science
Jiayu Wu, Yanan Wang, Yu An, Changyu Tian, Lingfeng Wang, Zuhong Liu, Desheng Qi
Summary: This study compared the gene expression differences in liver tissues of laying hens at different ages using RNA sequencing, and found that the transport and metabolism of amino acids changed during the growing period, which influenced the growth and development of the hens.
Article
Agriculture, Dairy & Animal Science
J. Raabe, G. Raveendran, W. Otten, K. Homeyer, T. Bartels
Summary: This study investigated the effects of different flicker frequencies on the behavior, performance, and stress response in male turkeys. The results showed that flicker frequencies of 165 Hz or higher had no detrimental effect on growth performance, injurious pecking, or endocrine stress response in the turkeys.
Article
Agriculture, Dairy & Animal Science
Adamu Mani Isa, Yanyan Sun, Yuanmei Wang, Yunlei Li, Jingwei Yuan, Aixin Ni, Hui Ma, Lei Shi, Hailai Hagos Tesfay, Yunhe Zong, Panlin Wang, Pingzhuang Ge, Jilan Chen
Summary: This study sequenced mRNAs and lncRNAs in the ovary stroma of specific chicken breeds and their reciprocal crossbreds to understand the molecular mechanism of heterosis for egg production. Results showed that genes with nonadditive expression were related to follicle growth and atresia as well as oxidative stress in the crossbred hens.
Article
Agriculture, Dairy & Animal Science
Zhepeng Wang, Qiu Chen, Yiwei Wang, Yulu Wang, Ruifang Liu
Summary: The study investigates the functional variants associated with chicken eggshell color in the SLCO1B3 gene by measuring the eggshell color of Lueyang black-boned chickens and analyzing the genetic data. It identifies 15 variants in SLCO1B3 that are significantly associated with eggshell color. However, the functional analysis shows that one specific variant, 1B3_SNP108, does not have a significant effect on promoter activity. This study highlights the importance of SLCO1B3 and its variants in breeding eggshell color.
Article
Agriculture, Dairy & Animal Science
P. Yin, Q. Tong, B. M. Li, W. C. Zheng, Y. Wang, H. Q. Peng, X. L. Xue, S. Q. Wei
Summary: This study investigated the welfare and health of laying hens in a multitier system. The results showed that the spatial distribution of hens changed with increasing age, and lower-ranking hens preferred to stay in lower tiers to avoid competition but still had access to resources. This improved the welfare and health of the hens near the end of the laying cycle.
Article
Agriculture, Dairy & Animal Science
Wenwen Liang, Siqiong Jie, Yiqin Wang, Xiaowei Wen, Yanling Xiong, Xiaoxi Lin, Zhengrong Lv
Summary: This study examined the effect of risk perceptions on preventive behavior among vendors in the live poultry wholesale market and the live poultry retail market in Guangdong Province using the health belief model and structural equation modeling. The results showed that perceived severity and perceived benefits positively influenced vendors' self-efficacy in the wholesale market, while perceived barriers negatively affected self-efficacy in both the wholesale and retail markets. Moreover, cues to action mediated the relationship between perceived severity, perceived benefits, and self-efficacy, and also mediated the effect of perceived susceptibility among wholesale market vendors.
Article
Agriculture, Dairy & Animal Science
Shreeya Sharma, Raveendra R. Kulkarni, Shayan Sharif, Hosni Hassan, Mohammadali Alizadeh, Scott Pratt, Khaled Abdelaziz
Summary: The study investigates the impact of exposing chick embryos to antigens via in ovo delivery of poultry-specific lactobacilli on the expression of genes associated with early bursal development and maturation. Results indicate that the supplementation of poultry-specific lactobacilli to chick embryos can accelerate the development and immunological maturation of the bursa of Fabricius.