Article
Hematology
Jesse I. Mobbs, Katrina A. Black, Michelle Tran, Wessel A. C. Burger, Hariprasad Venugopal, Theodore R. Holman, Michael Holinstat, David M. Thal, Alisa Glukhova
Summary: This study used single-particle cryo-electron microscopy to determine high-resolution structures of human 12-LOX, revealing its multiple oligomeric states and small molecule binding. The findings provide important insights into the assembly, catalytic mechanism, and drug development targeting the 12-LOX enzyme.
Article
Medicine, General & Internal
Saiyang Xie, Mengya Chen, Wenxi Fang, Shiqiang Liu, Qingqing Wu, Chen Liu, Yun Xing, Wenke Shi, Man Xu, Min Zhang, Si Chen, Xiaofeng Zeng, Shasha Wang, Wei Deng, Qizhu Tang
Summary: This study revealed the impact of Alox5 on pathological cardiac remodeling under hypertensive stimuli, demonstrating the protective effect of targeting Alox5 against cardiac remodeling and heart failure. The study also discovered that Alox5 exerts its effects by disturbing the liquid-liquid phase separation (LLPS) of Runx2 and reducing EGFR transcription activation.
Article
Biochemistry & Molecular Biology
Izumi Tsukayama, Yuki Kawakami, Asako Tamenobu, Keisuke Toda, Saya Maruoka, Yuki Nagasaki, Yoshiko Mori, Risa Sawazumi, Kensuke Okamoto, Keita Kanzaki, Hideyuki Ito, Yoshitaka Takahashi, Yoshimi Miki, Kei Yamamoto, Makoto Murakami, Toshiko Suzuki-Yamamoto
Summary: In this study, it was found that malabaricone C, a component of nutmeg, strongly inhibited the activity of 5-lipoxygenase, an enzyme involved in leukotriene biosynthesis. Furthermore, malabaricone C was shown to ameliorate psoriasis-like skin inflammation by decreasing the level of leukotriene B4.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Min-Ju Kim, Jin Lee, Su-Eun Kim, Kyung-Chul Shin, Deok-Kun Oh
Summary: The putative lipoxygenase from Shewanella hanedai was identified as arachidonate 9S-lipoxygenase. Optimization of conditions led to increased production of C20 9S- and C22 11S-hydroxy fatty acids.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Yvonne Benatzy, Megan A. Palmer, Bernhard Bruene
Summary: ALOX15B, a unique lipoxygenase enzyme, is associated with various diseases such as inflammation and cancer. It peroxidizes polyenoic fatty acids and generates corresponding hydroperoxides. ALOX15B plays a role in cellular cholesterol homeostasis and is induced by hypoxia.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Mohammad G. Sabbir, Jeffrey T. Wigle, Carla G. Taylor, Peter Zahradka
Summary: Endothelial cells regulate vascular homeostasis through secretion of bioactive lipids, and this study investigates the enzymes responsible for generating these lipids. The results show that ALOX15B and ALOXE3 play crucial roles in maintaining a healthy endothelium, with their expression levels influenced by proliferative status and shear stress.
Review
Biotechnology & Applied Microbiology
Ruth Chrisnasari, Marie Hennebelle, Jean -Paul Vincken, Willem J. H. van Berkel, Tom A. Ewing
Summary: This review focuses on the recent findings of the biochemical characteristics of bacterial LOXs and their molecular structures, exploring their potential applications in industrial processes and the approaches to improve their performance through mutagenesis.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Cell Biology
Yu-Ting Tsai, Wei-Lun Lo, Pin-Yuan Chen, Chiung-Yuan Ko, Jian-Ying Chuang, Tzu-Jen Kao, Wen-Bing Yang, Kwang-Yu Chang, Chia-Yang Hung, Ushio Kikkawa, Wen-Chang Chang, Tsung- Hsu
Summary: Sp1-regulated PGE2 production activates FAO and TCA cycle in mitochondria, resulting in TMZ resistance in GBM. These findings provide a new strategy to attenuate drug resistance or to re-sensitize recurrent GBM.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
K. E. Boesche, S. S. Donkin
Summary: The study explored the effects of different fatty acids on the activity of the bovine PCP1 promoter, with C18:3n-3 cis showing a greater impact on promoter activity and enhancing the activity when combined with C16:0.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Hematology
Hanny Al-Samkari, Kathryn Addonizio, Bertil Glader, D. Holmes Morton, Satheesh Chonat, Alexis A. Thompson, Kevin H. M. Kuo, Yaddanapudi Ravindranath, Heng Wang, Jennifer A. Rothman, Janet L. Kwiatkowski, Charles Kung, Penelope A. Kosinski, Hasan Al-Sayegh, Wendy B. London, Rachael F. Grace
Summary: Diagnosis of pyruvate kinase deficiency can be challenging, but the study found that the PK:HK ratio had excellent sensitivity for diagnosis. Furthermore, erythrocyte PK-R protein levels may be a useful prognostic biomarker for disease severity.
BRITISH JOURNAL OF HAEMATOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Seong-Eun Kim, Jin Lee, Jung-Ung An, Tae-Hun Kim, Chae-Won Oh, Yoon-Joo Ko, Manigandan Krishnan, Joonhyeok Choi, Do-Young Yoon, Yangmee Kim, Deok-Kun Oh
Summary: In this study, we identified a LOX from the bacterium Sphingopyxis macrogoltabida that is involved in the biosynthesis of SPMs, which act as signaling molecules in the resolution of inflammation and infection. By studying the enzyme's activity and mutant variants, we discovered the products catalyzed by the LOX and the specific residues involved in substrate selectivity. Furthermore, using chemical analysis techniques, we identified novel LMs with anti-inflammatory activities. This study provides new insights for physiological research.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Review
Oncology
Maryam Gholamalizadeh, Nazanin Majidi, Shirin Tajaddod, Sepideh Abdollahi, Seyed Mohammad Poorhosseini, Mina Ahmadzadeh, Mohammad Naimi Joubani, Samaneh Mirzaei Dahka, Hanieh Shafaei, Mogge Hajiesmaeil, Atiyeh Alizadeh, Saeid Doaei, Anahita Houshiar-Rad
Summary: This study investigated the mechanisms underlying the interaction between CRC, gene polymorphisms, and dietary fat. The results suggest that certain polymorphisms in the ALOX and COX genes may play a significant role in the association between CRC risk and dietary fats.
FRONTIERS IN ONCOLOGY
(2022)
Article
Pathology
Terufumi Kubo, Tomoki Kikuchi, Masahiko Obata, Takayuki Kanaseki, Tomohide Tsukahara, Yoshihiko Hirohashi, Shingo Ichimiya, Naoya Nakamura, Toshihiko Torigoe
Summary: This study examined the expression of ALOX5 in B-cell lymphomas and non-neoplastic lymphoid tissues. The findings showed that multiple types of B-cell lymphomas expressed ALOX5, while diffuse large B-cell lymphoma did not. These findings suggest that ALOX5 may be a novel marker for identifying the cell of origin of B-cell lymphoma.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Nutrition & Dietetics
Song Hee Lee, Bo Young Choi, A. Ra Kho, Dae Ki Hong, Beom Seok Kang, Min Kyu Park, Si Hyun Lee, Hui Chul Choi, Hong Ki Song, Sang Won Suh
Summary: The combined treatment of pyruvate and DCA shows protective effects against seizure-induced hippocampal neuronal cell death by increasing the proportion of pyruvate converted to ATP.
Article
Horticulture
Qing Chen, Yu Long, Min Yang, Hairong Wang, Yujie Sun, Xiaoyang Liu, Liangxin Wang, Guoyan Hou, Yuyan Jiang, Mengyao Li, Yong Zhang, Yuanxiu Lin, Yunting Zhang, Yan Wang, Wen He, Xiaorong Wang, Haoru Tang, Ya Luo
Summary: This study identified 53 pyruvate kinases (PKs) in the genome of octoploid strawberry, among which a mitochondrial pyruvate kinase (FaPKc2.2) was found to play a regulatory role in fruit ripening. FaPKc2.2 was inhibited by ABA and sucrose, and overexpression of this enzyme significantly inhibited strawberry fruit ripening. Metabolomic and transcriptomic data revealed that upregulation of FaPKc2.2 led to a reprogramming of metabolism, diverting the main carbon flux towards nitrogen-containing biomolecules.
SCIENTIA HORTICULTURAE
(2022)
Article
Agriculture, Dairy & Animal Science
Jesper Tessin, Judith Rohde, Arne Jung, Nicole Kemper, Jochen Schulz
Summary: In this study, samples from poultry house environment and culled birds were collected, and E. cecorum was successfully isolated. The principle of isolating the pathogen by streaking on a chromogenic agar may motivate researchers to investigate transmission routes and optimize biosecurity measures.
Article
Agriculture, Dairy & Animal Science
Junqin Zhang, Zimin Xie, Yanlin Pan, Zuoxin Chen, Yunzhen Huang, Linlin Li, Jiawen Dong, Yong Xiang, Qi Zhai, Minhua Sun, Xingying Li, Shujian Huang, Ming Liao
Summary: In recent years, there has been an increase in the occurrence of fowl adenovirus 2 (FAdV-2) in China, posing a significant threat to the poultry industry. This study investigated the epidemiology, phylogenetic relationship, genomic characteristics, and pathogenicity of FAdV-2. The results showed that FAdV-2 was the most commonly detected serotype among various FAdV serotypes. Genetic analysis revealed that these FAdV-2 strains formed a distinct branch within the FAdV-D group and had a high nucleotide similarity with reference strains. Recombination analysis identified recombination events between FAdV-2 and FAdV-11 in some strains. Pathogenicity experiments demonstrated that a recombinant FAdV-2 strain caused a high mortality rate and more severe disease in chickens. These findings contribute to our understanding of FAdV-2 and provide a basis for vaccine development.
Article
Agriculture, Dairy & Animal Science
Ebtsam Iraqi, Ali Abdel Hady, Nadia Elsayed, Hanaa Khalil, Amina El-Saadany, Karim El-Sabrout
Summary: Thermal manipulation during egg incubation, particularly at 12 to 18 days, has positive effects on embryonic development, hatching characteristics, chick quality, as well as posthatch thermotolerance and performance.
Article
Agriculture, Dairy & Animal Science
Yong Liu, Mingxin Song, He Bai, Chunhua Wang, Fei Wang, Qi Yuan
Summary: Dietary curcumin supplementation can significantly improve egg quality of quails in the late laying period, primarily by improving lipid metabolism and selectively regulating the intestinal microbial community.
Article
Agriculture, Dairy & Animal Science
Lei Zhang, Zhixuan Song, Peiyao Li, Xingju Song, Xinming Tang, Dandan Hu
Summary: This study evaluated the vaccine potential of recombinant proteins for avian coccidiosis caused by Eimeria. The results showed that the rEten5-B protein can trigger immune protection against E. tenella and may be a potential and effective subunit vaccine for the control of coccidiosis in poultry.
Article
Agriculture, Dairy & Animal Science
Milan Kumar Sharma, Amit Kumar Singh, Doyun Goo, Venkata Sesha Reddy Choppa, Hanseo Ko, Hanyi Shi, Woo Kyun Kim
Summary: The study investigates the effect of Eimeria infection on gastrointestinal physiology, growth performance, and egg production of Hy-Line W-36 laying hens during peak production. Results show that Eimeria infection negatively affects body weight, feed intake, immune response, and nutrient transporter gene expression. Egg production temporarily ceases in hens infected with high and medium-high dosages of Eimeria.
Article
Agriculture, Dairy & Animal Science
Elwy A. Ashour, Mahmoud Kamal, Hayman A. A. Altaie, Ayman A. Swelum, Gamaleldin M. Suliman, Guillermo Tellez-Isaias, Mohamed E. Abd El-Hack
Summary: This study evaluated the effects of CP and ME on the performance and nutritional value of Japanese quail lay chicken. The results showed that protein and energy levels significantly influenced all productive outcomes except feed conversion ratio. The combination of dietary energy and protein levels had a substantial effect on all egg quality trials evaluated. It is recommended to feed layer Japanese quail between the ages of 8 and 20 weeks during the summer with a dietary energy content of 2,900 kcal ME/kg and 20% CP.
Article
Agriculture, Dairy & Animal Science
J. E. Doornweerd, R. F. Veerkamp, B. de Klerk, M. van der Sluis, A. C. Bouwman, E. D. Ellen, G. Kootstra
Summary: Tracking group-housed individual broilers using video can provide valuable information on their health, welfare, and performance. This study conducted a comprehensive analysis on broiler tracking on video and potential tracking errors, highlighting the potential and challenges of phenotyping broiler locomotion. The results emphasized the importance of addressing tracking errors, potential algorithm improvements, and the need for an external animal identification system for efficient locomotion phenotyping.
Article
Agriculture, Dairy & Animal Science
Jiayu Wu, Yanan Wang, Yu An, Changyu Tian, Lingfeng Wang, Zuhong Liu, Desheng Qi
Summary: This study compared the gene expression differences in liver tissues of laying hens at different ages using RNA sequencing, and found that the transport and metabolism of amino acids changed during the growing period, which influenced the growth and development of the hens.
Article
Agriculture, Dairy & Animal Science
J. Raabe, G. Raveendran, W. Otten, K. Homeyer, T. Bartels
Summary: This study investigated the effects of different flicker frequencies on the behavior, performance, and stress response in male turkeys. The results showed that flicker frequencies of 165 Hz or higher had no detrimental effect on growth performance, injurious pecking, or endocrine stress response in the turkeys.
Article
Agriculture, Dairy & Animal Science
Adamu Mani Isa, Yanyan Sun, Yuanmei Wang, Yunlei Li, Jingwei Yuan, Aixin Ni, Hui Ma, Lei Shi, Hailai Hagos Tesfay, Yunhe Zong, Panlin Wang, Pingzhuang Ge, Jilan Chen
Summary: This study sequenced mRNAs and lncRNAs in the ovary stroma of specific chicken breeds and their reciprocal crossbreds to understand the molecular mechanism of heterosis for egg production. Results showed that genes with nonadditive expression were related to follicle growth and atresia as well as oxidative stress in the crossbred hens.
Article
Agriculture, Dairy & Animal Science
Zhepeng Wang, Qiu Chen, Yiwei Wang, Yulu Wang, Ruifang Liu
Summary: The study investigates the functional variants associated with chicken eggshell color in the SLCO1B3 gene by measuring the eggshell color of Lueyang black-boned chickens and analyzing the genetic data. It identifies 15 variants in SLCO1B3 that are significantly associated with eggshell color. However, the functional analysis shows that one specific variant, 1B3_SNP108, does not have a significant effect on promoter activity. This study highlights the importance of SLCO1B3 and its variants in breeding eggshell color.
Article
Agriculture, Dairy & Animal Science
P. Yin, Q. Tong, B. M. Li, W. C. Zheng, Y. Wang, H. Q. Peng, X. L. Xue, S. Q. Wei
Summary: This study investigated the welfare and health of laying hens in a multitier system. The results showed that the spatial distribution of hens changed with increasing age, and lower-ranking hens preferred to stay in lower tiers to avoid competition but still had access to resources. This improved the welfare and health of the hens near the end of the laying cycle.
Article
Agriculture, Dairy & Animal Science
Wenwen Liang, Siqiong Jie, Yiqin Wang, Xiaowei Wen, Yanling Xiong, Xiaoxi Lin, Zhengrong Lv
Summary: This study examined the effect of risk perceptions on preventive behavior among vendors in the live poultry wholesale market and the live poultry retail market in Guangdong Province using the health belief model and structural equation modeling. The results showed that perceived severity and perceived benefits positively influenced vendors' self-efficacy in the wholesale market, while perceived barriers negatively affected self-efficacy in both the wholesale and retail markets. Moreover, cues to action mediated the relationship between perceived severity, perceived benefits, and self-efficacy, and also mediated the effect of perceived susceptibility among wholesale market vendors.
Article
Agriculture, Dairy & Animal Science
Shreeya Sharma, Raveendra R. Kulkarni, Shayan Sharif, Hosni Hassan, Mohammadali Alizadeh, Scott Pratt, Khaled Abdelaziz
Summary: The study investigates the impact of exposing chick embryos to antigens via in ovo delivery of poultry-specific lactobacilli on the expression of genes associated with early bursal development and maturation. Results indicate that the supplementation of poultry-specific lactobacilli to chick embryos can accelerate the development and immunological maturation of the bursa of Fabricius.