Article
Polymer Science
Marika Faggioli, Arianna Moro, Salman Butt, Martina Todesco, Deborah Sandrin, Giulia Borile, Andrea Bagno, Assunta Fabozzo, Filippo Romanato, Massimo Marchesan, Saima Imran, Gino Gerosa
Summary: The most common aortic valve diseases in adults are stenosis and regurgitation. Pediatric patients have a lower incidence of aortic pathologies. Replacement of native valve with prosthetic valve requires consideration of durability. Both mechanical and biological prosthetic valves need to be replaced in pediatric patients. Acellular porcine aortic valve may be an alternative with lower immunogenic risk and longer lifespan. This study reports an efficient protocol for decellularization and evaluates the structural integrity, cell and nuclear component removal, and biomechanical properties.
Article
Polymer Science
Susanna Tondato, Arianna Moro, Salman Butt, Martina Todesco, Deborah Sandrin, Giulia Borile, Massimo Marchesan, Assunta Fabozzo, Andrea Bagno, Filippo Romanato, Saima Jalil Imran, Gino Gerosa
Summary: Right ventricle outflow tract obstruction (RVOTO), accounting for about 15% of congenital heart diseases, often requires pulmonary valve replacement. This study investigated the use of a new detergent (Tergitol) to decellularize porcine pulmonary valves for scaffold production. The decellularized scaffolds were evaluated for structural integrity, and their compatibility with mesenchymal stem cells was assessed. Tergitol efficiently removed nuclear material while preserving the matrix's structural proteins, making it a potential alternative to Triton X-100.
Article
Cell & Tissue Engineering
Atefeh Yaghoubi, Negar Azarpira, Saied Karbalay-Doust, Sajad Daneshi, Zahra Vojdani, Tahereh Talaei-Khozani
Summary: This study investigated the use of BM-MSC and prednisolone to reduce fibrosis and balance inflammatory cell migration. The results showed that loading prednisolone into the scaffold can effectively restrict inflammation, and the cell/prednisolone-loaded bioscaffold plays an important role in regeneration and angiogenesis.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Health Care Sciences & Services
Suncica Kapor, Milica Vukotic, Tijana Suboticki, Dragoslava Dikic, Olivera Mitrovic Ajtic, Milica Radojkovic, Vladan P. Cokic, Juan F. Santibanez
Summary: The study found that HU inhibits the growth of BMMSC, causing senescence-like changes and affecting cellular differentiation and immunoregulatory functions.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biophysics
Jiazhu Xu, Qi Fang, Yanli Liu, Yan Zhou, Zhaoyang Ye, Wen-Song Tan
Summary: Biomimetic instructive tissue engineering scaffolds are crucial for successful tissue regeneration. In this study, a novel scaffold was developed by coating PCL electrospun fibers with chondrocyte-derived ECM, facilitating chondrogenesis of MSCs. The dECM/PCL scaffolds exhibited distinct surface microstructural features with varying fiber diameters, promoting better adhesion, proliferation, and chondrogenic differentiation of rMSCs compared to uncoated PCL scaffolds.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Cell Biology
Ebtehal Ahmed, Tarek Saleh, Meifeng Xu
Summary: Researchers are exploring the use of mesenchymal stem cells (MSCs) as alternative cells for repopulating decellularized scaffolds. MSCs, derived from various sources, have therapeutic effects on a wide range of inflammatory/degenerative diseases. Overcoming the drawbacks of MSC recellularization is essential for generating bioengineered transplantable organs.
Review
Pharmacology & Pharmacy
Shuqin Zhang, Denghui Xie, Qun Zhang
Summary: This review summarizes the current status and prospects of bone regeneration therapy, introducing the roles of scaffolds and stem cells in tissue engineering, common treatment strategies for bone metabolism-related diseases, and the direction for further development.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Engineering, Biomedical
Victor I. I. Sevastianov, Yulia B. B. Basok, Alexey M. M. Grigoriev, Evgeny A. A. Nemets, Alexandra D. D. Kirillova, Liudmila A. A. Kirsanova, Aleksey E. E. Lazhko, Anastasia Subbot, Marina V. V. Kravchik, Yusef D. D. Khesuani, Elizaveta V. V. Koudan, Sergey V. V. Gautier
Summary: This study investigated the properties of decellularized porcine cartilage microparticles (DCMps) obtained through different physical treatments. The results showed that DCMps prepared with ultrasound treatment exhibited the best performance in terms of cell viability and chondroinduction, making them a promising source for cartilage reconstruction.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Multidisciplinary Sciences
Simon Decker, Marcela Arango-Ospina, Felix Rehder, Arash Moghaddam, Rolf Simon, Christian Merle, Tobias Renkawitz, Aldo R. Boccaccini, Fabian Westhauser
Summary: This study investigates the influence of boron-doped bioactive glasses (BGs) on angiogenesis and osteogenesis. The results show that boron doping has a slight stimulatory effect on in vitro cellular osteogenic differentiation and angiogenesis, while significantly enhancing vascularization in vivo. The implementation of new experimental models, such as extending the use of the chorioallantoic membrane (CAM) assay, is important for studying the 'angiogenic-osteogenic coupling' mechanisms.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Alina Ioana Ardelean, Madalina Florina Dragomir, Marioara Moldovan, Codruta Sarosi, Gertrud Alexandra Paltinean, Emoke Pall, Lucian Barbu Tudoran, Ioan Petean, Liviu Oana
Summary: Uniform filler distribution in composites is achieved by utilizing PCL microcapsules to release filler gradually during matrix polymerization. Two composites, C1 and C2, containing BisGMA, UDMA, HEMA, and PEG400 as well as previously described mineral filler, were prepared with different filler percentages. SEM observations revealed that C1 completely disintegrated the microcapsules during polymerization while C2 retained some embedded microcapsule parts alongside BaO filler particles. Palatal-origin mesenchymal stem cells cultured on both composites exhibited robust proliferation and demonstrated pluripotency stage followed by differentiation. The cytotoxicity tests and EDX spectra confirmed the biocompatibility and osteoinductive behavior of both C1 and C2 composites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Hideaki Kagami, Minoru Inoue, Hideki Agata, Izumi Asahina, Tokiko Nagamura-Inoue, Masataka Taguri, Arinobu Tojo
Summary: By optimizing the cell-processing protocol, stable bone regeneration can be achieved with comparable quality and less fluctuation.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Guillaume Rougier, Louis Maistriaux, Lies Fieve, Daela Xhema, Robin Evrard, Julie Manon, Raphael Olszewski, Fabien Szmytka, Nicolas Thurieau, Jean Boisson, Natacha Kadlub, Pierre Gianello, Catherine Behets, Benoit Lengele
Summary: This paper investigates the possibility of creating a living bone allograft using the perfusion/decellularization/recellularization (PDR) technique. The results show that the decellularized vascularized bone grafts successfully preserve the 3D morphology and extracellular matrix (ECM) microarchitecture, promote cell growth and differentiation, and offer new perspectives for repairing large bone defects and bone tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Engineering, Biomedical
Heng Zhou, Yan He, Wei Xiong, Shuili Jing, Xingxiang Duan, Zhiyun Huang, Gurek S. Nahal, Youjian Peng, Mingchang Li, Yaoqi Zhu, Qingsong Ye
Summary: Mesenchymal stem cells (MSCs) have promising potential for neural regeneration therapy due to their plasticity and accessibility. However, the therapeutic efficacy of MSCs alone is often inadequate, leading to the use of genetic engineering to enhance their abilities. Different methods of gene transfer have specific advantages and disadvantages. MSCs can also be modified to improve their neural repair abilities. This review focuses on gene transport technologies for engineering MSCs and the selection of optimal delivery genes, as well as the prospects and challenges of their application in animal models of neurological diseases.
BIOACTIVE MATERIALS
(2023)
Article
Polymer Science
Laura Martinez-Vidal, Valentina Magno, Petra B. Welzel, Jens Friedrichs, Martin Bornhaeuser, Carsten Werner
Summary: This study explores the use of modular macroporous cryogels seeded with mesenchymal stromal cells and decellularized to generate tissue-specific extracellular matrix (ECM)-decorated scaffolds to mimic the bone marrow niche. The addition of macromolecular crowders to the cell culture medium helps recreate the physiological concentration of biomolecules. The ECM-coated cryogels generated under crowding conditions exhibit physicochemical properties similar to those of the endosteal niche of the bone marrow, and could be used in the future for HSC expansion and disease model development.
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2023)
Article
Engineering, Multidisciplinary
Foteini K. Kozaniti, Despina D. Deligianni, Margarita D. Georgiou, Diana Portan
Summary: The hypothesis of this research is that modifying the substrate's topography and/or stiffness to mimic biological conditions can influence cell behavior. Substrates with similar surface chemistry but varying stiffness and topographies were created, and their effects on Wharton's Jelly Umbilical Cord Mesenchymal Stem Cells were investigated. The results showed that while solvent casting scaffolds had better mechanical properties, the biomimetic fibrous texture of the electrospun substrates improved cell viability and proliferation. Cell adhesion and differentiation were more pronounced on solvent casting substrates. Soft substrates enhanced cell multiplication and migration, while stiff substrates induced differentiation into bone cells.