Article
Biochemistry & Molecular Biology
Hongyan Su, Jie Li, Sijin Chen, Ping Sun, Hua Xing, Delong Yang, Xiaona Zhang, Mengfei Li, Jianhe Wei
Summary: Through physiological and transcriptomic analysis of Hypericum perforatum grown at different temperatures, it was found that the stomatal density, opening percentage, and Hyp content were significantly higher at lower temperatures compared to higher temperatures. A total of 150 genes were associated with Hyp biosynthesis, plant growth, and stress response processes, providing insight into the regulation mechanisms of low temperature enhancing Hyp biosynthesis in Hypericum perforatum.
Article
Plant Sciences
Hongyan Su, Ling Jin, Mengfei Li, Paul W. Pare
Summary: Plants of St John's wort grown at cooler temperatures exhibit changes in leaf anatomy and gene expression, which support the plant's ability to accumulate enhanced levels of the anti-depression compound hypericin at low temperatures.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agricultural Engineering
Masumeh Karimi, Nima Ahmadi, Morteza Ebrahimi
Summary: The study revealed that increasing the red light fraction in LED light had a positive effect on the growth, flowering, and secondary metabolite accumulation in Hypericum perforatum plants, while higher levels of blue light showed poorer growth and flowering traits. Additionally, antioxidant enzymes activities, total phenolic and flavonoid content were higher in plants exposed to a mixture of red and blue light compared to those grown under white LED light.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Shamshad Ahmad Khan, Priyanka Verma, Varsha A. Parasharami
Summary: Overexpression of the HpPKS2 gene in H. perforatum leads to higher hypericin content in the native system, while its expression in the heterologous system B. monnieri also improves bacopaside I, bacopaside II, and bacopasaponin C production.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Biochemical Research Methods
Borivoj Adnadjevic, Biljana Koturevic, Jelena Jovanovic
Summary: This study aimed to investigate the kinetics of extracting total hypericin from Hypericum perforatum flowers, finding that the Jander three-dimensional diffusion model best described the extraction process. The calculation of kinetic parameters and identification of the controlling step provide important insights for the extraction process.
PHYTOCHEMICAL ANALYSIS
(2021)
Article
Plant Sciences
Qian Zhang, Wen Zhou, Bin Li, Lin Li, Meng Fu, Li Zhou, Xiaoding Yu, Donghao Wang, Zhezhi Wang
Summary: By analyzing the genome of Hypericum perforatum, 101 ERF genes were identified and a systematic bioinformatic analysis was conducted, leading to the classification of HpERFs into 11 subfamilies. Most HpERFs were found to participate in plant biological processes, with cis-elements related to gene transcription regulation, stress response, and plant development. This study provides a theoretical basis for further research on stress-related ERFs in H. perforatum.
Article
Biochemistry & Molecular Biology
Wen Zhou, Shu Yang, Lei Yang, Ruyi Xiao, Shiyi Chen, Donghao Wang, Shiqiang Wang, Zhezhi Wang
Summary: WRKY is a large transcription factor family in plants, playing an important regulatory role in plant responses to various stresses, especially drought stress. However, there is no comprehensive analysis of this family in H. perforatum, which is widely studied and known for its medicinal properties. In this study, 86 HpWRKY genes were identified, classified and analyzed. It was found that HpWRKY85 showed significant responses to drought treatment, and its overexpression in Arabidopsis enhanced root growth and scavenging of reactive oxygen species. These findings provide insights into the molecular mechanism of drought resistance in H. perforatum.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Maryam Saffariha, Ali Jahani, Reza Jahani, Sajid Latif
Summary: The MLP model (R-2=0.87) was found to be more accurate in predicting hypericin content in H. perforatum compared to RBF (R-2=0.8) and SVM (R-2=0.54) models. Sensitivity analysis revealed that phenological stages, hill aspects, total nitrogen, altitude, and organic carbon are the most influential factors affecting hypericin content in the plant.
Article
Biochemistry & Molecular Biology
Martina Paponov, Manya Antonyan, Rune Slimestad, Ivan A. Paponov
Summary: The study investigated the effects of the plant hormone jasmonic acid and far-red light signaling on plant growth and secondary metabolite accumulation, revealing that they mainly act independently without observable interactions. The combined application of jasmonic acid and far-red light enhanced the accumulation of most secondary compounds without compromising plant growth.
Article
Biochemistry & Molecular Biology
Jie Zhang, Chunrong Feng, Pu Ge, Qingqing Wang, Yanling Liu, Hui Xu, Xing Zhou
Summary: The study demonstrated a facile strategy for the separation and purification of hypericin from Hypericum perforatum L. extract using MRCC and PLC techniques. Various parameters of MRCC were investigated to optimize the separation efficiency of hypericin, while PLC parameters were determined to achieve high purity hypericin with a high recovery ratio.
PROCESS BIOCHEMISTRY
(2021)
Article
Engineering, Biomedical
Rosmeire Aparecida Barroso, Ricardo Navarro, Carla Roberta Tim, Lucas de Paula Ramos, Luciane Dias de Oliveira, Angela Toshie Araki, Karina Gonzales Camara Fernandes, Daniela Macedo, Livia Assis
Summary: Acne vulgaris is a common skin condition that can be effectively reduced by antimicrobial photodynamic therapy, particularly when using 15 μg/mL of hypericin combined with 3 and 5 J of low-level laser irradiation. This combination showed a significant reduction in the P. acnes biofilms, demonstrating effective antimicrobial action.
LASERS IN MEDICAL SCIENCE
(2021)
Article
Plant Sciences
Masumeh Karimi, Nima Ahmadi, Morteza Ebrahimi
Summary: The content of secondary metabolites in medicinal plants is influenced by light quality, with red LED promoting growth and blue LED causing growth retardation. Different light spectra have significant regulatory effects on plant growth and yield.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Chemistry, Medicinal
Fakry F. Mohamed, Darisuren Anhlan, Michael Schoefbaenker, Andre Schreiber, Nica Classen, Andreas Hensel, Georg Hempel, Wolfgang Scholz, Joachim Kuehn, Eike R. Hrincius, Stephan Ludwig
Summary: A study found that the extract of Hypericum perforatum possesses potent antiviral activity against SARS-CoV-2, with naphtodianthrones hypericin and pseudohypericin playing a crucial role. This research highlights the potential value of Hypericum perforatum and its active ingredients in combating SARS-CoV-2 infections.
Article
Biochemistry & Molecular Biology
Surbhi Kapoor, Rajat Chandel, Ramandeep Kaur, Satish Kumar, Raj Kumar, Sandeep Janghu, Amarjeet Kaur, Vikas Kumar
Summary: In recent years, globalization has led to the growth of the food processing industry to meet the increasing demand for various food products. This has also renewed interest in medicinal plants, with Hypericum perforatum L. being one of them. The flower of this plant species contains bioactive compounds that have potential uses in the nutraceutical and pharmaceutical sectors. Research has shown that it possesses various health-promoting properties, such as antidepressant, antioxidant, and anti-inflammatory effects, making it a potential ingredient in food and pharmaceutical products.
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
(2023)
Article
Multidisciplinary Sciences
Li Zhou, Xiaoding Yu, Donghao Wang, Lin Li, Wen Zhou, Qian Zhang, Xinrui Wang, Sumin Ye, Zhezhi Wang
Summary: Heat shock transcription factors (HSFs) play critical roles in regulating plant responses to various stresses, including high temperature stress, by controlling the expression of heat shock proteins (HSPs). The recently sequenced H. perforatum genome provided valuable genetic and functional resources for identifying and analyzing 23 putative HpHSF genes. Analysis of HpHSFs' gene structure and conserved motifs indicated specific phylogenetic relationships and suggested their potential regulatory functions in abiotic stress responses, especially in heat stress tolerance.
Article
Plant Sciences
Fanli Jing, Yongping Miao, Peipei Zhang, Tao Chen, Yuan Liu, Jingfu Ma, Mengfei Li, Delong Yang
Summary: Sucrose-phosphatase (SPP) plays a crucial role in sucrose biosynthesis in wheat crops. Genetic variations in TaSPP-5A gene can affect sucrose content and yield in wheat. The TaSPP-5Aa haplotype is associated with higher sucrose content and grain weight, potentially beneficial for enhancing grain yield.
Article
Biochemistry & Molecular Biology
Jie Wang, Hongyan Su, Hongping Han, Wenshu Wang, Mingcong Li, Yubi Zhou, Yi Li, Mengfei Li
Summary: This study investigated the growth characteristics and polysaccharides biosynthesis of Cynomorium songaricum in different hosts. It found that C. songaricum hosted in Nitraria roborowskii exhibited higher fresh weight, soluble sugar content, and antioxidant capacity compared to those hosted in Nitraria sibirica. Analysis of differentially expressed genes revealed some genes associated with polysaccharides and starch metabolism.
Article
Biochemistry & Molecular Biology
Tiantian Zhu, Minghui Zhang, Hongyan Su, Meiling Li, Yuanyuan Wang, Ling Jin, Mengfei Li
Summary: In this study, metabolomic and transcriptomic analyses were conducted to compare the differences of flavonoids and transcripts in two cultivars of Angelica sinensis. The results showed higher levels of flavonoids and anthocyanins in the cultivar with purple stem (M1) compared to the cultivar with green stem (M2). Several candidate genes involved in flavonoid biosynthesis were identified. These findings provide references for flavonoid production and cultivar selection of A. sinensis.
Article
Biochemistry & Molecular Biology
Han Dong, Meiling Li, Ling Jin, Xiaorong Xie, Mengfei Li, Jianhe Wei
Summary: The study revealed that cooler temperature enhances the growth of Angelica sinensis, as well as the accumulation of ferulic acid and flavonoids, while inhibiting polysaccharide biosynthesis. These findings confirm the determining role of cool temperature in geo-authentic formation and provide a foundation for regulating metabolites production in Angelica sinensis.
Article
Plant Sciences
Meiling Li, Xiuwen Cui, Ling Jin, Mengfei Li, Jianhe Wei
Summary: This study investigated the root biomass, accumulations of ferulic acid, flavonoids, and lignin, antioxidant capacity, and related gene expression during four different bolting stages in Angelica sinensis. The results showed a decrease in root biomass, ferulic acid, and flavonoids, while an increase in lignin content during the bolting stages. Antioxidant capacity also decreased with growth and development. The differential expression of genes involved in the biosynthesis of ferulic acid, flavonoids, and lignin correlated with changes in metabolite accumulation. These findings provide useful references for improving the production of bioactive metabolites in A. sinensis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Green & Sustainable Science & Technology
Qiong Jia, Mengfei Li, Xuecheng Dou
Summary: Climate factors have a significant impact on crop production potential in the arid and semi-arid regions of northwest China. Precipitation plays a more important role than other climate factors in improving crop production potential. Although there are positive correlations, actual crop yields do not have a significant relationship with crop production potential.
Article
Plant Sciences
Sijin Chen, Hongyan Su, Hua Xing, Juan Mao, Ping Sun, Mengfei Li
Summary: This study investigated the differential expression of proteins in the roots of cold-resistant and cold-sensitive grapevine varieties under different low temperatures using proteomic analysis. The results showed that the root activity of the cold-resistant variety was higher and decreased with decreasing temperature. A total of 25 differentially co-expressed proteins were involved in stress response, bio-signaling, metabolism, energy, and translation.
Article
Plant Sciences
Mimi Luo, Xiaoxia Liu, Hongyan Su, Meiling Li, Mengfei Li, Jianhe Wei
Summary: A transcriptomic analysis was conducted on Angelica sinensis using RNA-seq, revealing key genes involved in flowering regulation during vernalization and providing new insights into the regulatory networks of flowering in Angelica sinensis.
Article
Biochemistry & Molecular Biology
Jie Wang, Hongyan Su, Zhibo Wu, Wenshu Wang, Yubi Zhou, Mengfei Li
Summary: This study investigates the physiological characteristics and transcriptomics of Cynomorium songaricum during different growth stages, showing that stem biomass, soluble sugar and flavonoid content, and antioxidant capacity peak at the early flowering stage. Key genes involved in polysaccharide and flavonoid biosynthesis, as well as growth and development, were found to be crucial in the accumulation and regulation of these bioactive compounds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Qiaozhu Zhao, Miaoyin Dong, Mengfei Li, Ling Jin, Paul W. Pare
Summary: In this study, the relationship between light intensity and flavonoid accumulation in Sinopodophyllum hexandrum at high altitudes was investigated. It was found that higher light intensity was associated with increased levels of flavonoids, flavonols, and anthocyanins. Proteomic and transcriptomic analyses revealed the up-regulation of specific proteins and genes involved in flavonoid biosynthesis and light stress response at higher altitudes. These findings suggest that light intensity plays a regulatory role in enhancing flavonoid accumulation in S. hexandrum.
Article
Agricultural Engineering
Tianmiao Huang, Di Liu, Xiuwen Cui, Meiling Li, Ling Jin, Paul W. Pare, Mengfei Li, Jianhe Wei
Summary: This study successfully cultivated seedlings of the traditional Chinese medicine Angelica sinensis by inducing embryogenic callus, suspension cell culture, bioactive metabolite determination, and plantlet acclimatization. The highest growth rate and bioactive metabolite contents were observed under the optimal treatment conditions, leading to the successful establishment of robust plants.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Xin Li, Jinjuan Li, Hongyan Su, Ping Sun, Zhen Zhang, Mengfei Li, Hua Xing
Summary: This study investigated the physiological and transcriptional changes during seed germination of Apocynum venetum under different NaCl treatments. The results showed that low NaCl concentrations promoted seed germination rate, while high concentrations inhibited it. Antioxidant enzyme activity increased significantly in the range of 0-150 mmol/L NaCl and decreased in the range of 150-300 mmol/L. Osmolyte content increased significantly with increased concentrations, while protein content peaked at 100 mmol/L NaCl and then significantly decreased.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Meiling Li, Min Li, Li Wang, Mengfei Li, Jianhe Wei
Summary: This paper summarizes the application of Apiaceae plants in traditional Chinese medicine, including the removal of dampness, relief of superficies, and dispelling cold. It also summarizes the traditional use, modern pharmacological use, phytochemistry, effect of bolting and flowering (BF), and approaches for controlling BF of these medicinal plants. Currently, 228 Apiaceae medicinal plants have been recorded as Traditional Chinese Medicines (TCMs), with 6 medicinal parts, 79 traditional uses, 62 modern pharmacological uses, and 5 main metabolites. The review provides useful references for the reasonable exploration and high-quality production of these plants.
Review
Biochemistry & Molecular Biology
Miaoyin Dong, Jinjuan Li, Delong Yang, Mengfei Li, Jianhe Wei
Summary: Astragalus membranaceus, a well-known traditional herbal medicine, has been used for more than 2000 years and its bioactive compounds have shown various pharmacological effects, such as liver protection, immunomodulation, and anti-cancer activities. This review comprehensively discusses the biosynthesis pathway of flavonoids and triterpenoid saponins in Astragalus membranaceus, as well as the structural features of polysaccharides. The review also summarizes the pharmacological effects of these compounds, providing a foundation for the development of clinical candidate agents. Additionally, potential strategies for heterologous biosynthesis are proposed to improve the industrial production and supply of natural products from Astragalus membranaceus.
Article
Biochemistry & Molecular Biology
Xiaoxia Liu, Mimi Luo, Mengfei Li, Jianhe Wei
Summary: In this study, long noncoding RNAs (lncRNAs) associated with flowering in Angelica sinensis were identified through full-length transcriptomic analysis. The results showed that these lncRNAs positively or negatively regulate the flowering of A. sinensis, providing new insights into the regulatory mechanism of flowering in this plant.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Plant Sciences
Wanli You, Jinglin Zhang, Xueyin Ru, Feng Xu, Zhengguo Wu, Peng Jin, Yonghua Zheng, Shifeng Cao
Summary: This study investigated the effect of calcium chloride (CaCl2) treatment on GABA accumulation in fresh-cut cantaloupe and the underlying mechanisms. The results showed that CaCl2 treatment increased GABA content and the activities of GAD and SSADH enzymes, while reducing glutamate content and GABA-T activity. Additionally, CaCl2 treatment upregulated the expressions of CmCML11 and CmCAMTA5, as well as several GABA shunt genes, through the transcriptional activation by CmCAMTA5. Furthermore, the interaction between CmCML11 and CmCAMTA5 enhanced the transcriptional activation of GABA shunt genes. Overall, this study reveals that CaCl2 treatment promotes GABA accumulation in fresh-cut cantaloupe through the combined effect of CmCML11 and CmCAMTA5 in regulating the expressions of GABA shunt genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Min Wang, Yupeng Wang, Xiaohui Wang, Guangwei Wei, Huiyi Yang, Xi Yang, Tinghai Shen, Huijie Qu, Sheng Fang, Ziming Wu
Summary: This study identified the high nitrogen use efficiency (NUE) black sesame variety 17-156 and analyzed its underlying physiological and molecular mechanisms. The results showed that 17-156 possesses a sophisticated nitrogen metabolizing machinery to uptake and assimilate higher quantities of inorganic nitrogen, simultaneously improving carbon metabolism and growth. Many important genes were up-regulated in 17-156 under high nitrogen condition. Additionally, 38 potential candidate genes were identified for future studies to improve sesame's NUE. These findings provide valuable resources for understanding the regulatory network of nitrogen metabolism and developing sesame cultivars with improved NUE.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
He Dong, Chongmei Xu, Chengtao Zhang, Li Zhang, Yaqin Yao, Suiqi Zhang
Summary: The study found that short cells in maize leaves not only improve leaf mechanical support and photosynthetic performance, enhance drought resistance, but also participate in stomatal regulation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Na Liu, Wenyan Shang, Mengxin Guan, Jibin Xiao, Guangxiang Tian, Baozhan Ma, Wenjing Shang, Xu Li, Shijia Zhao, Chuang Li, Kun Cheng, Wenming Zheng
Summary: This study cloned the full-length cDNA sequence of TaSPX3 gene in wheat and found that TaSPX3 responds to low phosphorus stress in multiple wheat genotypes. Overexpressing TaSPX3 can alleviate phosphorus deficiency symptoms and promote plant growth in Arabidopsis. The study also revealed the interaction of TaSPX3 with other genes related to the phosphorus starvation signaling pathway.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Kongyuan Wu, Lizhen Wang, Zihan Wu, Ziqing Liu, Zengfei Li, Jun Shen, Shengjie Shi, Hong Liu, Christopher Rensing, Renwei Feng
Summary: Selenium (Se) can reduce uptake and translocation of cadmium (Cd) in plants by regulating root morphology. This study investigated the effects of Se(IV) on root exudates, root morphology, root endogenous hormones, and Cd uptake efficiency in rice under Cd stress. The results showed that Se(IV) significantly reduced Cd concentrations in shoots and roots, and decreased Cd uptake efficiency via root hairs. Se(IV) also affected root morphology, root exudates, and the synthesis of hormones like IAA and JA. However, transcriptome analysis revealed no upregulated differentially expressed genes (DEGs) in IAA synthesis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Di Wang, Huaifang Zhang, Xuefei Hu, Haizhen Zhang, Shuang Feng, Aimin Zhou
Summary: This study identified a cell number regulator gene called SlCNR8 in willow, which enhances resistance to trace metals in transgenic poplar seedlings. SlCNR8 reduces Cd uptake and accumulation, and can be used as a candidate gene for genetic improvement of phytostabilisation of trace metals.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
