Journal
OPTICS EXPRESS
Volume 27, Issue 14, Pages 19950-19972Publisher
Optica Publishing Group
DOI: 10.1364/OE.27.019950
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Funding
- National Institutes of Health (NIBIB) [P41-EB002025]
- National Institute of General Medical Science [GM-R35GM122596]
- National Science Foundation [DGE-1650116, DMS-1361375]
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We developed VIEW-MOD (Versatile Illumination Engine with a Modular Optical Design): a compact, multi-modality microscope, which accommodates multiple illumination schemes including variable angle total internal reflection, point scanning and vertical/horizontal light sheet. This system allows combining and flexibly switching between different illuminations and imaging modes by employing three electrically tunable lenses and two fast-steering mirrors. This versatile optics design provides control of 6 degrees of freedom of the illumination source (3 translation, 2 tilt, and beam shape) plus the axial position of the imaging plane. We also developed standalone software with an easy-to-use GUI to calibrate and control the microscope. We demonstrate the applications of this system and software in biosensor imaging, optogenetics and fast 3D volume imaging. This system is ready to fit into complex imaging circumstances requiring precise control of illumination and detection paths, and has a broad scope of usability for a myriad of biological applications. (c) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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