Article
Biotechnology & Applied Microbiology
Yuanyuan Shi, Yingxin Wang, Zhaochen Shan, Zhenhua Gao
Summary: Decellularized submandibular gland extracellular matrix (DSMG) was found to have similar structure and composition to dental pulp. DSMG could serve as an alternative scaffold for dental pulp regeneration, supporting adhesion and proliferation of dental pulp stem cells. Implanted cell-seeded DSMG formed a vascularized dental pulp-like tissue expressing markers involved in dentinogenesis and angiogenesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Qian Zhang, Yixin Hu, Xuan Long, Lingling Hu, Yu Wu, Ji Wu, Xiaobing Shi, Runqi Xie, Yu Bi, Fangyuan Yu, Pinxue Li, Yu Yang
Summary: Cartilage regeneration relies on cellular-ECM interactions. Traditional tissue engineering methods have not been able to replicate the physiological structure of natural cartilage, but recent advances in ECM-based scaffolds show promise in improving cartilage regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ali Doryab, Otmar Schmid
Summary: Chronic lung diseases are a leading cause of death worldwide, and lung transplantation is currently the only treatment option but limited by donor availability. Lung tissue engineering offers a promising approach to regenerate damaged lung tissue. Researchers have developed an extracellular matrix-integrated scaffold that closely mimics the microenvironment of lung cells and shows potential for lung tissue engineering applications.
Article
Engineering, Biomedical
Yi Li, Qiong Wu, Li Li, Fei Chen, Ji Bao, Weimin Li
Summary: Utilizing a cocktail of dextrose, SLES, and Triton X-100 proved to be the most effective method for decellularizing porcine whole lung, preserving ECM composition while minimizing residual cellular components and xenoantigens. Evaluation through staining and immunofluorescence confirmed the noticeable preservation of ECM ultrastructure in the SLES-treated groups. The developed protocol holds promise for generating clinical-scale bioengineered lung scaffolds.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Engineering, Biomedical
Gilles S. van Tienderen, Oskar Rosmark, Ruby Lieshout, Jorke Willemse, Floor de Weijer, Linda Elowsson Rendin, Gunilla Westergren-Thorsson, Michail Doukas, Bas Groot Koerkamp, Martin E. van Royen, Luc J. W. van der Laan, Monique M. A. Verstegen
Summary: Patient-derived tumor organoids were combined with native tumor and liver scaffolds through decellularization to study the interaction between tumor cells and the extracellular matrix (ECM). The use of decellularized scaffolds resulted in a more accurate representation of the in vivo tumor environment and increased chemoresistance. This method also revealed environment-dependent proliferation dynamics and stimulated the production of collagen types.
ACTA BIOMATERIALIA
(2023)
Article
Nanoscience & Nanotechnology
My Thi Ngoc Nguyen, Ha Le Bao Tran
Summary: The study aimed to prepare injectable acellular adipose matrix (AAM) through delipidating and decellularizing, showing that mechanical disruption improved fat removal efficiency and the decellularization strategy was effective. AAM powder was non-toxic to human adipose derived stem cells and supported cell migration and proliferation.
JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES
(2021)
Article
Chemistry, Multidisciplinary
Lisa Sassi, Omolola Ajayi, Sara Campinoti, Dipa Natarajan, Claire McQuitty, Riccardo Rayan Siena, Sara Mantero, Paolo De Coppi, Alessandro F. Pellegata, Shilpa Chokshi, Luca Urbani
Summary: Decellularised organ scaffolds in the field of in vitro liver disease models maintain original properties, but the scarcity of appropriate bioreactors hampers tissue engineering approaches. A novel specific bioreactor was designed for long-term 3D culture of whole liver constructs, supporting cell survival and function. The bioreactor allows non-invasive monitoring and analysis of bioengineered constructs, showing higher viability, distribution, and functionality under dynamic conditions compared to static culture.
Review
Medicine, General & Internal
Yujia Shang, Guanhuier Wang, Yonghuan Zhen, Na Liu, Fangfei Nie, Zhenmin Zhao, Hua Li, Yang An
Summary: This review provides an overview of the application of the decellularization-recellularization technique in the field of plastic and reconstructive surgery, highlighting its potential in promoting the regeneration of diseased or damaged tissues and organs by using decellularized extracellular matrix as a scaffold.
CHINESE MEDICAL JOURNAL
(2023)
Article
Engineering, Biomedical
Charlot Philips, Lisanne Terrie, Lieven Thorrez
Summary: A review is presented on the progress and challenges in skeletal muscle decellularization, discussing different decellularization methods and applications of the scaffolds. Decellularized skeletal muscle has been primarily studied for regenerative purposes, focusing on tissue-specific morphological features and biochemical cues for muscle regeneration. However, this review expands the potential applications of decellularized skeletal muscle to demonstrate its versatility as a biomaterial.
Article
Engineering, Biomedical
Katsuhiro Tomofuji, Ken Fukumitsu, Jumpei Kondo, Hiroshi Horie, Kenta Makino, Satoshi Wakama, Takashi Ito, Yu Oshima, Satoshi Ogiso, Takamichi Ishii, Masahiro Inoue, Etsuro Hatano
Summary: In this study, researchers reconstructed intrahepatic bile ducts in a rat decellularized liver graft using organoid technology. Through an ex vivo perfusion culture system, they successfully demonstrated the biliary characteristics of repopulated liver organoids and reconstructed biliary tree-like networks with luminal structures. They also established a method to mimic the appropriate cell distribution in the native liver and demonstrated reconstructed bile ducts in human organoids.
Article
Engineering, Biomedical
Dafydd O. Visscher, Hyeongjin Lee, Paul P. M. van Zuijlen, Marco N. Helder, Anthony Atala, James J. Yoo, Sang Jin Lee
Summary: The study developed a printable bioink cdECMMA derived from porcine auricular cartilage, which exhibited good rheological properties and mechanical integrity. Chondrocytes in the printed cdECMMA hydrogel maintained viability, proliferation capacity, and produced cartilage ECM components. The novel cell-based bioprinting using this cartilage-specific dECMMA bioink offers an alternative option for auricular cartilage reconstruction.
ACTA BIOMATERIALIA
(2021)
Article
Biotechnology & Applied Microbiology
Sara Lopez-Martinez, Hannes Campo, Lucia de Miguel-Gomez, Amparo Faus, Alfredo T. Navarro, Ana Diaz, Antonio Pellicer, Hortensia Ferrero, Irene Cervello
Summary: Decellularization techniques were used to create biocompatible extracellular matrix hydrogels from porcine endometrium, providing a supportive environment for in vitro cell culture and tissue regeneration. The EndoECM exhibited tissue-specific features and supported the culture of human endometrial cells, showing potential for endometrial pathologies treatment. This study presents new strategies in reproductive techniques and endometrial repair using biomimetic endometrial milieus.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ifeolu Akinnola, Daniel R. Rossi, Carolyn Meyer, Ashley Lindsey, Douglas R. Haase, Samuel Fogas, Michael J. Ehrhardt, Rachel E. Blue, Andrew P. Price, Max Johnson, Diego F. Alvarez, Doris A. Taylor, Angela Panoskaltsis-Mortari
Summary: This study evaluated the recellularization of blood vessel conduits of acellular lung scaffolds with different types of endothelial cells, showing that RMEPCs have the capability to repopulate all vascular segments, while PAECs and MVECs exhibit selective tropism for larger vessels or microvasculature, respectively. The RMEPC phenotype described in the study could be valuable for identifying suitable endothelial progenitors for efficient vascular organ and tissue engineering, regeneration, and repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Evan T. Hoffman, Franziska E. Uhl, Loredana Asarian, Bin Deng, Chloe Becker, Juan J. Uriarte, Isaac Downs, Brad Young, Daniel J. Weiss
Summary: Chronic lung diseases, such as COPD and IPF, exhibit region-specific changes in extracellular matrix (ECM) composition, particularly in collagen and basement-membrane associated proteins. This study compares the ECM composition of whole decellularized lungs and specific anatomical lung regions in non-diseased, COPD, and IPF human lungs. The findings demonstrate distinct ECM signatures in different regions and reveal alterations in ECM composition specific to COPD and IPF, including enrichment of type-III collagen and fibulin in IPF aECM. This study provides methodology for future research and a dataset for identifying novel ECM targets for therapeutics.
Article
Cell Biology
Silvia Barbon, Andrea Biccari, Elena Stocco, Giovanni Capovilla, Edoardo D'Angelo, Martina Todesco, Deborah Sandrin, Andrea Bagno, Filippo Romanato, Veronica Macchi, Raffaele De Caro, Marco Agostini, Stefano Merigliano, Michele Valmasoni, Andrea Porzionato
Summary: Esophageal reconstruction using bio-engineered allografts that resemble the tissue extracellular matrix (ECM) properties is a promising strategy. This study successfully decellularized human esophagus using three detergent-enzymatic protocols and demonstrated preservation of ECM components, retention of collagen and elastin, and reduced immunogenicity. It also showed changes in tissue stiffness but maintained sample integrity. The study highlights the potential of using decellularized esophageal matrices for future tissue repopulation and orthotopic implantation.
Review
Surgery
Eiji Kobayashi, Toshiaki Shichinohe
Summary: This article reports on the contents and conflicts of interest of Cadaver Surgical Training (CST) programs in Japan based on professional autonomy. The Japan Surgical Society CST Promotion Committee evaluated the validity of the reports from participating universities and provided advice on proper implementation of CST. Over the past decade, 38 universities have been using cadavers for clinical education and research, such as CST. In case of identified inappropriate entries or unclear areas, the universities could be asked to resubmit their reports. This review clarifies the evaluation points of the CST reporting system, presents the status and problems of its reporting, and provides suggestions for future dissemination of CST.
Editorial Material
Orthopedics
Takane Suzuki, Toshiaki Shichinohe, Eiji Kobayashi
JOURNAL OF ORTHOPAEDIC SCIENCE
(2023)
Article
Multidisciplinary Sciences
Momoko Hirano, Kazuhisa Sugai, Masahiko Fujisawa, Eiji Kobayashi, Yoshinori Katsumata, Yoji Hakamata, Motoaki Sano
Summary: The intraperitoneal administration of hydrogen-rich saline can suppress neuronal cell death caused by ischemic stroke, even though hydrogen hardly reaches the brain.
Article
Biochemistry & Molecular Biology
Naoto Matsumoto, Shuichiro Yamanaka, Keita Morimoto, Kenji Matsui, Sandy Nishimura, Yoshitaka Kinoshita, Yuka Inage, Koki Fujimori, Takao Kuroda, Yatsumu Saito, Tsuyoshi Takamura, Toshinari Fujimoto, Susumu Tajiri, Kei Matsumoto, Makoto Inoue, Eiji Kobayashi, Takashi Yokoo
Summary: The number of patients with end-stage renal failure is increasing annually worldwide, but there is a shortage of renal transplantation donors. Transplanting renal progenitor cells into heterologous fetuses has been shown to induce the development of nephrons. In this study, researchers successfully generated human-mouse chimeric kidneys by combining this technology with drug-induced removal of specific renal progenitor cells in transgenic mice. They also developed a model using re-aggregation of mouse renal progenitor cells to evaluate the chimerogenic potential of human nephron progenitor cells derived from pluripotent stem cells. This study provides promising potential for the evaluation and generation of chimeric kidneys for regenerative medicine.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Immunology
Syuhei Yoshimoto, Akihiko Soyama, Masayuki Fukumoto, Takanobu Hara, Masaaki Hidaka, Shinji Torai, Hiroo Kasamatsu, Jun Ishikawa, Masayuki Ohara, Eiji Kobayashi, Susumu Eguchi
Summary: This study explored the feasibility of using livers from uncontrolled donors after circulatory death (u-DCD) through transplantation. A pig model simulating u-DCD was used, and liver function was evaluated after 24 hours of perfusion. The results showed that u-DCD livers can be successfully transplanted using ex vivo machine perfusion.
TRANSPLANTATION PROCEEDINGS
(2023)
Article
Immunology
Shinji Torai, Kanta Kurauchi, Eiji Kobayashi
Summary: This study presented a newly developed organ protector called OrganPocket and demonstrated its effectiveness in mitigating SWI injury in clinical kidney transplantation. The results showed that using OrganPocket significantly reduced organ temperature and alleviated SWI injury.
TRANSPLANTATION PROCEEDINGS
(2023)
Article
Immunology
Kentaro Ide, Hiroshi Sakai, Ryosuke Nakano, Yuki Imaoka, Naoki Tanimine, Ryuta Ide, Naofumi Tsukiyama, Kosuke Ono, Tetsuya Mochizuki, Ryosuke Arata, Keishi Hakoda, Koki Imaoka, Sotaro Fukuhara, Tomoaki Bekki, Hiroyuki Tahara, Masahiro Ohira, Eiji Kobayashi, Hideki Ohdan
Summary: This study examined the efficacy of a thermal barrier bag in kidney transplantation. The results showed that the bag effectively reduced the temperature of the transplanted kidney, leading to better preservation of kidney function and improved transplant outcomes.
TRANSPLANTATION PROCEEDINGS
(2023)
Article
Biochemistry & Molecular Biology
Yuka Nozaki, Masaki Kobayashi, Hiroki Wakasawa, Shunsuke Hoshino, Fumika Suwa, Yuko Ose, Ryoma Tagawa, Yoshikazu Higami
Summary: Obesity is a metabolic disorder associated with various diseases. WWP1, a HECT-type E3 ubiquitin ligase, is involved in multiple diseases. In a mouse model of obesity, the level of WWP1 was found to be increased in white adipose tissue, and obese Wwp1 knockout mice showed improved glucose metabolism. These findings suggest that WWP1 plays a role in obesity-related metabolic dysfunction and hepatic steatosis via suppressed insulin signaling.
Article
Medicine, General & Internal
Kenji Matsui, Yoshitaka Kinoshita, Yuka Inage, Naoto Matsumoto, Keita Morimoto, Yatsumu Saito, Tsuyoshi Takamura, Hitomi Matsunari, Shuichiro Yamanaka, Hiroshi Nagashima, Eiji Kobayashi, Takashi Yokoo
Summary: Fetal porcine kidneys injected with human nephron progenitor cells could be a viable option for kidney transplantation; using a model of freezing and transplantation, we demonstrated that the differentiation potency of vitrified fetal porcine kidneys could be evaluated, providing a practical protocol to assess the quality of individual lots.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Transplantation
Kentaro Ide, Ryosuke Nakano, Yuki Imaoka, Hiroshi Sakai, Kosuke Ono, Naoki Tanimine, Hiroyuki Tahara, Masahiro Ohira, Keiko Ueda, Taizo Hirata, Eiji Kobayashi, Hideki Ohdan
Summary: The thermal barrier bag (TBB) can maintain a low temperature for transplanted kidneys during vascular anastomosis, leading to improved outcomes for kidney transplant recipients.
TRANSPLANTATION DIRECT
(2023)
Article
Engineering, Biomedical
Hidenori Tani, Eiji Kobayashi, Shinomi Yagi, Keisuke Tanaka, Kotaro Kameda-Haga, Shinsuke Shibata, Nobuko Moritoki, Kaworu Takatsuna, Taijun Moriwaki, Otoya Sekine, Tomohiko C. Umei, Yuika Morita, Yusuke Soma, Yoshikazu Kishino, Hideaki Kanazawa, Jun Fujita, Shunji Hattori, Shugo Tohyama
Summary: This study found that heart-derived collagen is more effective in heart tissue engineering and can lead to more mature engineered heart tissue.
Review
Pathology
Yuina Otani, Yuka Nozaki, Yuhei Mizunoe, Masaki Kobayashi, Yoshikazu Higami
Summary: Long-term caloric restriction is a well-established intervention to improve metabolism, suppress age-related pathophysiology, and extend lifespan. It can be regulated by both growth hormone/insulin-like growth factor 1-dependent and -independent mechanisms. This review focuses on the molecular mechanisms of caloric restriction regulated in a growth hormone/insulin-like growth factor 1-independent manner, especially in white adipose tissue.
PATHOLOGY INTERNATIONAL
(2023)
Editorial Material
Biochemistry & Molecular Biology
Asahi Oda, Yoji Hakamata, Eiji Kobayashi
Summary: Blackberries contain important substances that have antioxidative and other effects, and efforts are being made to enhance their absorption in the body. Through studies using animal models, it has been found that pre-administration of blackberry extracts can have positive effects on the animals before an ischemic event.
Article
Multidisciplinary Sciences
Yuhei Higashi, Jun Homma, Hidekazu Sekine, Hiroki Yago, Eiji Kobayashi, Tatsuya Shimizu
Summary: This study proposes a method of improving the circulatory distribution of organs during perfusion by applying intermittent air pressure from outside. Experimental results demonstrate that this method can improve the circulatory distribution of organs, reduce tissue damage, and maintain the ability to filter perfusate.
SCIENTIFIC REPORTS
(2022)
Review
Medicine, Research & Experimental
Shinya Sugimoto, Eiji Kobayashi, Takanori Kanai, Toshiro Sato
Summary: The establishment of an organoid culture system has advanced our understanding of intestinal epithelium biology. Xenotransplantation of human intestinal organoids onto mouse colons provides insights into human disease biology and organoid-based regenerative medicine.
KEIO JOURNAL OF MEDICINE
(2022)
