Article
Biochemistry & Molecular Biology
Jessie W. Brunner, Hanna C. A. Lammertse, Annemiek A. van Berkel, Frank Koopmans, Ka Wan Li, August B. Smit, Ruud F. Toonen, Matthijs Verhage, Sophie van der Sluis
Summary: This study compares different designs and statistical analysis methods used in iPSC research, and finds that commonly used case-control designs are generally underpowered. The study suggests that multiple isogenic pair designs can increase power and require fewer lines. A free web tool is provided for exploring the power of different study designs using any (pilot) data.
MOLECULAR PSYCHIATRY
(2023)
Review
Toxicology
Xi Yang, Alexandre J. S. Ribeiro, Li Pang, David G. Strauss
Summary: Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are increasingly used in regulatory applications to assess drug-induced cardiotoxicity. However, the predictive capacity of hiPSC-CMs for human cardiac safety in clinical trials is still limited.
TOXICOLOGICAL SCIENCES
(2022)
Article
Toxicology
Feng Wei, Lisa Pence, Kellie Woodling, Prathyusha Bagam, Richard Beger, Goncalo Gamboa da Costa, Li Pang
Summary: This study compared the electrophysiological responses of hiPSC-CMs to different compounds in serum and serum-free media and explained the reason behind the differences. The study revealed the impact of hydrophobic compound loss in serum-free preparations on drug bioavailability and risk prediction of arrhythmia.
TOXICOLOGICAL SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Giovanna Menduti, Marina Boido
Summary: In the field of neurodegenerative pathologies, patient-derived induced pluripotent stem cells (iPSCs) provide a valuable tool for disease modelling and drug screening. The combination of high-throughput screening and subcellular resolution microscopy allows for detailed analysis of cell morphology and neurite trafficking in iPSC-derived neuronal cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Wener Li, Xiaojing Luo, Ying Ulbricht, Kaomei Guan
Summary: This study improved the method for single iPSC-CM preparation by applying 2.5 μm blebbistatin throughout the procedure, leading to significant improvements in cell morphology and electrical recording. The automated patch-clamp technique accurately recapitulated the electrophysiological phenotypes of iPSC-CMs derived from Brugada syndrome patients, demonstrating its potential to replace manual patch-clamp in modeling cardiac channelopathies with higher throughput.
STEM CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Ichiro Fukunaga, Yoko Oe, Keiko Danzaki, Sayaka Ohta, Cheng Chen, Kyoko Shirai, Atsushi Kawano, Katsuhisa Ikeda, Kazusaku Kamiya
Summary: This study successfully generated human iPSC-derived functional CX26 gap junction-forming cells, which have characteristics similar to cochlear supporting cells, providing new in vitro models for optimizing therapies and drug screening for hearing loss.
HUMAN MOLECULAR GENETICS
(2021)
Review
Materials Science, Multidisciplinary
Mark Jolly, Laurens Katgerman
Summary: Over the last four decades, significant progress has been made in the modelling of casting processes, enabling a better understanding of physical phenomena and calculation of difficult-to-measure quantities. However, there is still room for improvement in accurately predicting casting performance and defects.
PROGRESS IN MATERIALS SCIENCE
(2022)
Article
Multidisciplinary Sciences
Toqa Darwish, Azhar Al-Khulaifi, Menatalla Ali, Rana Mowafy, Abdelilah Arredouani, Suhail A. Doi, Mohamed M. Emara
Summary: This meta-analysis compared the responsiveness of animal models and induced pluripotent stem cells (iPSCs) to cAMP analogues in cystic fibrosis research. The study found considerable heterogeneity in both types of models and suggested the need for reproducible iPSC models to fully explore their potential.
Article
Geosciences, Multidisciplinary
Junjie Liu, Latha Baskaran, Kevin Bowman, David Schimel, A. Anthony Bloom, Nicholas C. Parazoo, Tomohiro Oda, Dustin Carroll, Dimitris Menemenlis, Joanna Joiner, Roisin Commane, Bruce Daube, Lucianna Gatti, Kathryn McKain, John Miller, Britton B. Stephens, Colm Sweeney, Steven Wofsy
Summary: The dataset CMS-Flux NBE 2020 provides global and regionally resolved terrestrial net biosphere exchange estimates from 2010 to 2018, assimilating CO2 observations from GOSAT and OCO-2. The dataset shows that tropical regions are close to neutral while extra-tropics are a net sink, confirmed by comparisons with aircraft and NOAA observations. The unique perspective provided by the dataset helps understand terrestrial biosphere carbon dynamics and changes in regional contributions to atmospheric CO2 growth rate.
EARTH SYSTEM SCIENCE DATA
(2021)
Review
Cell Biology
Eamonn A. A. Gaffney, Kenta Ishimoto, Benjamin J. J. Walker
Summary: The study of sperm motility dynamics began in the 1950s, highlighting the need for motors distributed along the flagellum rather than solely relying on molecular motors in the cell membrane. Recent advancements in digitization of sperm videomicroscopy, modeling, and methodology have brought forth a new era of scientific discoveries in this field.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Genetics & Heredity
Erin C. Hedges, Graham Cocks, Christopher E. Shaw, Agnes L. Nishimura
Summary: (English Summary)
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease that causes loss of control over voluntary movement. This study created an ALS iPSC biobank from patients carrying specific gene mutations, providing a disease-relevant platform for ALS research. Using patient-derived iPSCs, the study demonstrated the ability to replicate early ALS-related phenotypes, which can aid in the development of novel treatment strategies.
Article
Biochemistry & Molecular Biology
Paul E. Sladen, Katarina Jovanovic, Rosellina Guarascio, Daniele Ottaviani, Grace Salsbury, Tatiana Novoselova, J. Paul Chapple, Patrick Yu-Wai-Man, Michael E. Cheetham
Summary: Autosomal dominant optic atrophy (DOA) is the most common inherited optic neuropathy, characterized by the preferential loss of retinal ganglion cells (RGCs), resulting in optic nerve degeneration and progressive bilateral central vision loss. There are currently no licensed disease-modifying therapies for DOA and the disease mechanisms driving RGC degeneration are poorly understood.
HUMAN MOLECULAR GENETICS
(2022)
Article
Chemistry, Multidisciplinary
Adam R. Hill, Pablo Cubillas, James T. Gebbie-Rayet, Mollie Trueman, Nathan de Bruyn, Zulaikha al Harthi, Rachel J. S. Pooley, Martin P. Attfield, Vladislav A. Blatov, Davide M. Proserpio, Julian D. Gale, Duncan Akporiaye, Bjornar Arstad, Michael W. Anderson
Summary: CrystalGrower is a simulation tool that can model both crystal habits and nanoscale surface topography simultaneously, suitable for crystal growth simulation under variable supersaturation conditions. The tool is based on nanoscale coarse graining, faithfully reproducing the characteristics of crystals under low supersaturation or undersaturation conditions, and can simulate screw dislocations and internal point defects as well.
Article
Instruments & Instrumentation
Luka Lambrecht
Summary: Data quality monitoring and data certification are crucial for advanced detectors like CMS. The current manual approach requires a large amount of person power, so we investigate computer-assisted methods and use a case study in the pixel tracker to demonstrate the effectiveness of autoencoder techniques in spotting anomalous detector behavior with a previously inaccessible time granularity.
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT
(2023)
Article
Cell & Tissue Engineering
Alice Vickers, Mukul Tewary, Anna Laddach, Martina Poletti, Vasiliki Salameti, Franca Fraternali, Davide Danovi, Fiona M. Watt
Summary: The study investigated the impact of rare, deleterious non-synonymous single nucleotide variants (nsSNVs) in cell adhesion and germ layer development genes on the differentiation phenotypes of induced pluripotent stem cells (iPSCs). Although all iPSC lines differentiated into the three germ layers, there was donor-specific variation in germ layer patterning, with one line showing an outlier phenotype of expanded endodermal differentiation associated with a nsSNV in ITGB1. This study established a platform for further research on the influence of nsSNVs on cellular differentiation.
Article
Cardiac & Cardiovascular Systems
Paloma Nieto-Marin, David Tinaquero, Raquel G. Utrilla, Jorge Cebrian, Andres Gonzalez-Guerra, Teresa Crespo-Garcia, Anabel Camara-Checa, Marcos Rubio-Alarcon, Maria Dago, Silvia Alfayate, David Filgueiras, Rafael Peinado, Jose Luis Lopez-Sendon, Jose Jalife, Juan Tamargo, Juan Antonio Bernal, Ricardo Caballero, Eva Delpon
Summary: TBX5 variants identified in this study are associated with and modulate the electrical phenotype in patients with LQTS and BrS, highlighting their impact on cardiac conduction and action potentials.
CARDIOVASCULAR RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Alvaro Macias, J. Jaime Diaz-Larrosa, Yaazan Blanco, Victor Fanjul, Cristina Gonzalez-Gomez, Pilar Gonzalo, Maria Jesus Andres-Manzano, Andre Monteiro da Rocha, Daniela Ponce-Balbuena, Andrew Allan, David Filgueiras-Rama, Jose Jalife, Vicente Andres
Summary: This study found that heart tissue from Hutchinson-Gilford progeria syndrome (HGPS) patients has structural and electrophysiological defects, including disruption of the t-tubular system, shortened sarcomeres, bradycardia, and atrio-ventricular conduction abnormalities. Chronic treatment with low-dose paclitaxel partially corrected these abnormalities.
CARDIOVASCULAR RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Fumiya Tamura, Shintaro Sugimoto, Mana Sugimoto, Kazuho Sakamoto, Masahiko Yamaguchi, Takeshi Suzuki, Keiichi Fukuda, Masaki Ieda, Junko Kurokawa
Summary: The synthetic estrogen ethinylestradiol (EE2) did not significantly affect hERG currents at supratherapeutic concentrations but reduced the degree of hERG current suppression by E-4031 at therapeutic concentrations. The findings suggest a potential interaction between EE2 and E-4031 altering hERG function at the drug-binding site.
Article
Cardiac & Cardiovascular Systems
Giulio La Rosa, Jorge G. Quintanilla, Ricardo Salgado, Juan Jose Gonzalez-Ferrer, Victoria Canadas-Godoy, Julian Perez-Villacastin, Nicasio Perez-Castellano, Jose Jalife, David Filgueiras-Rama
Summary: Catheter ablation is the most effective and relatively minimally invasive therapy for rhythm control in patients with AF, providing significant improvement in patients' quality of life. However, it is not exempt from potential risk of complications and pro-arrhythmia.
EUROPEAN CARDIOLOGY REVIEW
(2021)
Article
Physiology
Colin H. Peters, Christian Rickert, Stefano Morotti, Eleonora Grandi, Kurt A. Aronow, Kurt G. Beam, Catherine Proenza
Summary: The funny current (I-f) plays a critical role in cardiac pacemaking and is also essential for the fight-or-flight response, contributing to the increase in heart rate.
JOURNAL OF GENERAL PHYSIOLOGY
(2022)
Article
Neurosciences
Xianwei Zhang, Charlotte E. R. Smith, Stefano Morotti, Andrew G. Edwards, Daisuke Sato, William E. Louch, Haibo Ni, Eleonora Grandi
Summary: This study investigates the independent and interactive effects of TATS and Ca2+-handling protein remodelling on Ca2+-handling abnormality driven membrane instabilities. The results show that the impact of protein remodelling on Ca2+-driven proarrhythmic behaviour varies depending on TATS density.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Cardiac & Cardiovascular Systems
Jordi Heijman, Xiaobo Zhou, Stefano Morotti, Cristina E. Molina, Issam H. Abu-Taha, Marcel Tekook, Thomas Jespersen, Yiqiao Zhang, Shokoufeh Dobrev, Hendrik Milting, Jan Gummert, Matthias Karck, Markus Kamler, Ali El-Armouche, Arnela Saljic, Eleonora Grandi, Stanley Nattel, Dobromir Dobrev
Summary: This study found that patients with atrial fibrillation (AF) have higher SK-channel current (I-SK) in their heart cells, and the antiarrhythmic drug apamin can significantly prolong the action potential of these cells. In addition, the study also found that the abundance of SK2 channel on the cell membrane is higher in AF patients. It was discovered that inhibiting the phosphatase-2A-dependent calmodulin-Thr80 dephosphorylation and promoting the trafficking and targeting of SK-channel subunits can enhance the function of SK channels.
CIRCULATION RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Nathaniel T. Herrera, Xianwei Zhang, Haibo Ni, Mary M. Maleckar, Jordi Heijman, Dobromir Dobrev, Eleonora Grandi, Stefano Morotti
Summary: Using a multiscale modeling framework, this study investigates the role of small-conductance Ca2+-activated K+ channels in regulating cell function and the development of Ca2+-driven arrhythmias in human atrial cardiomyocytes. The results suggest that while inhibiting these channels can counteract reentry-promoting effects, it may also increase vulnerability to delayed afterdepolarizations, potentially leading to triggered activity.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Meeting Abstract
Biophysics
Shan Lu, Milca A. Girmai, Donald M. Bers, Stefano Morotti
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Haibo Ni, Di Lang, Stefano Morotti, Alexey V. Glukhov, Eleonora Grandi
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Colin H. Peters, Christian Rickert, Stefano Morotti, Pin Liu, Stephanie Gantz, Bruce P. Bean, Eleonora Grandi, Cathy Proenza
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Noah Wiesner, Bardia Ghayoumi, Haibo Ni, Bence Hegyi, Stefano Morotti, Donald M. Bers, Ye Chen-Izu, Eleonora Grandi, Daisuke Sato
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Xianwei Zhang, Charlotte Smith, Stefano Morotti, Eleonora Grandi, Haibo Ni
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Xianwei Zhang, Haibo Ni, Stefano Morotti, Charlotte Smith, Daisuke Sato, William E. Louch, Andrew G. Edwards, Eleonora Grandi
BIOPHYSICAL JOURNAL
(2022)
Article
Cardiac & Cardiovascular Systems
Alba Alvarez-Franco, Raquel Rouco, Rafael J. Ramirez, Guadalupe Guerrero-Serna, Maria Tiana, Sara Cogliati, Kuljeet Kaur, Mohammed Saeed, Ricardo Magni, Jose Antonio Enriquez, Fatima Sanchez-Cabo, Jose Jalife, Miguel Manzanares
Summary: Transcriptomic and proteomic analysis of AF progression in a large animal model shows significant changes occurring at early stages, including previously undescribed increase in mitochondria, changes to the chromatin of atrial CMs, and genes related to neural function and cell proliferation.
CARDIOVASCULAR RESEARCH
(2021)