Review
Cell & Tissue Engineering
Li Zhou, Huimin Zhu, Xue Bai, Jiagui Huang, Yue Chen, Jun Wen, Xuemei Li, Bowen Wu, Yongjun Tan, Mingfen Tian, Jiangxia Ren, Mengxia Li, Qin Yang
Summary: Ischemic stroke is a major cause of death and disability. Stem cell transplantation has emerged as a potential treatment that offers hope for ischemic stroke patients.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Quan-Wen Liu, Qi-Ming Huang, Han-You Wu, Guo-Si-Lang Zuo, Hao-Cheng Gu, Ke-Yu Deng, Hong-Bo Xin
Summary: Stem cell therapy has the potential to repair tissues and improve functional recovery, but faces challenges such as ethical barriers and immune rejection. Human amnion-derived stem cells (hADSCs) show advantages over other stem cells, including high pluripotency and immunomodulatory effects, making them a promising source for cell therapy and regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Rui Alvites, Mariana Branquinho, Ana C. Sousa, Bruna Lopes, Patricia Sousa, Ana Colette Mauricio
Summary: Mesenchymal Stem/Stromal Cells (MSCs) have high clinical value and have been extensively studied. Their immunomodulatory influence occurs through both direct and paracrine routes, but the mechanisms are not fully understood. This review summarizes the immunoregulatory capacity of MSCs and their secretion products, with a focus on migration, homing, direct cell action, and paracrine activity, and explores related techniques.
Review
Oncology
Yaolu Wei, Yan Li, Yenan Chen, Pei Liu, Sheng Huang, Yuping Zhang, Yanling Sun, Zhe Wu, Meichun Hu, Qian Wu, Hongnian Wu, Fuxing Liu, Tonghui She, Zhifeng Ning
Summary: This review discusses the relationship and pathway of ALDH1 with various solid tumors. It proposes that ALDH1 may serve as a diagnosis and therapeutic target for CSC, providing new insights and new strategies for reliable tumor treatment.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell & Tissue Engineering
Tianli Yang, Feng Zhao, Liuhua Zhou, Jingyu Liu, Luwei Xu, Quanliang Dou, Zheng Xu, Ruipeng Jia
Summary: MSCs are a therapeutic tool for tissue engineering, with recent studies showing that their therapeutic effects are mediated by paracrine signaling and secretory factors, particularly exosomes. A novel cell-free therapy strategy utilizing exosomes, such as the AMEs mentioned in the study, has shown promising results in promoting tissue regeneration and functional recovery in a rat bladder replacement model.
JOURNAL OF TISSUE ENGINEERING
(2021)
Review
Cell & Tissue Engineering
Aline Queiroz, Emmanuel Albuquerque-Souza, Leticia Miquelitto Gasparoni, Bruno Nunes de Franca, Cibele Pelissari, Marilia Trierveiler, Marinella Holzhausen
Summary: PDLSCs are considered a promising cell source for regenerating periodontium and have the potential to be used as an effective treatment for periodontitis.
WORLD JOURNAL OF STEM CELLS
(2021)
Review
Cell Biology
Raheleh Farahzadi, Behnaz Valipour, Soheila Montazersaheb, Ezzatollah Fathi
Summary: Adult stem cells (ASCs) throughout the body can support tissue growth and repair. The function of stem cells is influenced by signals derived from their specific microenvironment or niche. Studies have shown that aging of the niche can lead to a decline in stem cell function and is associated with aging and age-related disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Genetics & Heredity
Lina Moallemi Rad, Alexey Yumashev, Bashdar Mahmud Hussen, Hazha Hadayat Jamad, Soudeh Ghafouri-Fard, Mohammad Taheri, Samaneh Rostami, Vahid Niazi, Mohammadreza Hajiesmaeili
Summary: Mesenchymal stem cells (MSCs) and MSC-derived exosomes (MSC-DEs) have therapeutic potential in ocular diseases by promoting eye injury repair and immune modulation.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Dong-Sik Chae, Sang Joon An, Seongho Han, Sung-Whan Kim
Summary: This study investigates the synergistic benefits of using dual 3D MSC culture systems to promote vascular regeneration and enhance therapeutic potential. The results demonstrate that dual 3D cultures of MSCs show higher expression of vascular growth factors and have better migration and tube formation abilities. In a mouse model, transplantation of dual 3D MSCs prevents limb loss and increases blood perfusion. The transplanted cells also show higher capillary density, angiogenic cytokine levels, and transdifferentiation into endothelial-like cells in the muscle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Jeremy Boulestreau, Marie Maumus, Christian Jorgensen, Daniele Noel
Summary: Osteoarthritis is associated with a high number of senescent cells in joint tissues, which release senescence-associated secretory phenotype (SASP) factors contributing to the disease progression. Targeting senescent cells has shown improvement in OA features in mouse models. MSCs and MSC-EVs are promising treatments for OA, but their functions are affected by aging.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Review
Polymer Science
Harish K. Handral, Tom Adam Wyrobnik, Alan Tin-Lun Lam
Summary: Microcarriers (MCs) are versatile therapeutic tools used in regenerative medicine and drug delivery. They can expand therapeutic cells, serve as tissue engineering scaffolds, and carry drugs and therapeutic compounds. Biodegradable MCs offer solutions to production challenges and can deliver cells at target sites without compromising quality or yield.
Article
Medicine, Research & Experimental
Yi Luan, Mingli Li, Yi Zhao, Qianqian Li, Jia Wen, Siqi Gao, Yang Yang
Summary: ***
The centrosome is a special organelle in human cells, with abnormalities potentially promoting cancer progression by affecting processes like mitosis and cell migration. Further research into the impact of centrosome abnormalities on tumors may help identify new therapeutic strategies.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Cell Biology
Yuxuan Zhong, Xiang Li, Fanglin Wang, Shoushuai Wang, Xiaohong Wang, Xiaohong Tian, Shuling Bai, Di Miao, Jun Fan
Summary: Recent studies have shown that various exosomes can influence the adipogenic differentiation of stem cells. Exosomes play an important role in promoting tissue regeneration and provide new ideas for adipose tissue engineering research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Vincent Kuek, Anastasia M. Hughes, Rishi S. Kotecha, Laurence C. Cheung
Summary: Uniform prospective clinical trials have improved remission rates and survival for patients with acute myeloid leukaemia and acute lymphoblastic leukaemia in recent decades. However, high-risk patients continue to have inferior outcomes due to mechanisms where leukaemic cells hijack the bone marrow microenvironment. Targeting the cellular interactions within the leukaemia-bone marrow niche is an exciting novel strategy with significant potential to enhance treatment efficacy and improve patient outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Yibing Chen, Huan Jin, Yucen Song, Ting Huang, Jun Cao, Qing Tang, Zhengzhi Zou
Summary: Tumor-associated macrophages play a significant role in tumor progression and treatment resistance in solid tumors, leading to the development of promising immunotherapeutic strategies targeting macrophages. Targeting TAMs in combination with conventional therapies has shown to be effective in treating solid tumors. Challenges, obstacles in clinical trials, and future perspectives for TAMs-targeting therapies in various cancers are also discussed in this review.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Oncology
Buddhadev Layek, Mihir Shetty, Susheel Kumar Nethi, Drishti Sehgal, Timothy K. Starr, Swayam Prabha
Review
Pharmacology & Pharmacy
Buddhadev Layek, Bina Gidwani, Sakshi Tiwari, Veenu Joshi, Vishal Jain, Amber Vyas
CURRENT PHARMACEUTICAL DESIGN
(2020)
Article
Medicine, Research & Experimental
Sanjay Arora, Buddhadev Layek, Jagdish Singh
Summary: This study demonstrated efficient brain-targeted delivery of ApoE2 gene therapy using surface-modified liposomes, with significantly higher ApoE2 expression in neurons and potential for effective AD treatment.
MOLECULAR PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Shen Cheng, Susheel Kumar Nethi, Mahmoud Al-Kofahi, Swayam Prabha
Summary: Nano-engineered mesenchymal stem cells (nano-MSCs) are potentially effective targeted drug delivery platforms for treating solid tumors, but their pharmacokinetic-pharmacodynamic relationship requires quantitative description to optimize therapeutic efficacy. A mechanism-based preclinical PK-PD model was developed to characterize the PK-PD relationship of nano-MSCs in orthotopic A549 human lung tumors in SCID Beige mice, showing potential for improving anti-tumor efficacy with dosage adjustments and modulation of exocytosis rates.
Review
Biochemistry & Molecular Biology
Divya Sharma, Sanjay Arora, Jagdish Singh, Buddhadev Layek
Summary: Gene therapy aims to transfer genetic materials into targeted cells for disease treatment or prevention. Nonviral vectors have gained attention for their safety, payload capacity, and stealth abilities, but face challenges in overcoming intracellular and extracellular barriers. This review highlights current advancements in nonviral gene delivery systems and their future potential.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Richard N. L. Lamptey, Bivek Chaulagain, Riddhi Trivedi, Avinash Gothwal, Buddhadev Layek, Jagdish Singh
Summary: Neurodegenerative disorders are characterized by neuron loss. Most current treatments only alleviate associated symptoms due to the limited effectiveness of drugs crossing the blood-brain barrier. Nanoparticles offer a promising approach for targeted drug delivery to the brain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Shen Cheng, Darcy R. Flora, Allan E. Rettie, Richard C. Brundage, Timothy S. Tracy
Summary: This study characterizes the impact of CYP2C9 genotype on drug-drug interactions between warfarin and fluconazole or rifampin. The results suggest that different dose adjustments may be required for patients with different CYP2C9 genotypes when warfarin is administered with either CYP inhibitors or inducers.
DRUG METABOLISM AND DISPOSITION
(2022)
Article
Pharmacology & Pharmacy
Shen Cheng, Darcy R. Flora, Allan E. Rettie, Richard C. Brundage, Timothy S. Tracy
Summary: The objective of this study was to analyze the population pharmacokinetics of 10 warfarin metabolites under different conditions. The analysis showed that CYP2C9 mediated elimination pathways were more inhibitable by fluconazole and less inducible by rifampin compared to non-CYP2C9 mediated elimination pathways. The study also found that for subjects with CYP2C9 *2 and *3 variants, non-CYP2C9 mediated elimination was more important than CYP2C9 mediated elimination.
DRUG METABOLISM AND DISPOSITION
(2022)
Article
Pharmacology & Pharmacy
Siddhee A. Sahasrabudhe, Shen Cheng, Mahmoud Al-Kofahi, Jeanine R. Jarnes, Neal J. Weinreb, Reena Kartha
Summary: This study aimed to develop and validate a physiologically-based pharmacokinetic model for eliglustat with and without drug interactions, and to simulate untested interaction scenarios. The study found that patients with CYP2D6 deficiency may benefit from lower doses of eliglustat.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2022)
Review
Biochemistry & Molecular Biology
Bivek Chaulagain, Avinash Gothwal, Richard Nii Lante Lamptey, Riddhi Trivedi, Arun Kumar Mahanta, Buddhadev Layek, Jagdish Singh
Summary: Central nervous system (CNS) disorders are a major global health burden, yet the approval rate for new therapies against these disorders is low compared to other diseases. The lack of reliable and efficient in vitro blood-brain barrier (BBB) models hinders the development of successful therapy for CNS disorders. This review discusses the major in vitro BBB models, including 2D transwell models, 3D organoid models, and microfluidic models, for exploring the barrier properties of the cerebral vasculature. Although microfluidic models better recapitulate the in vivo properties of BBB, significant gaps still exist for their use in predicting the performance of neurotherapeutics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Richard Nii Lante Lamptey, Chengwen Sun, Buddhadev Layek, Jagdish Singh
Summary: Hypertension is a global health concern, and neurogenic hypertension (NH) accounts for almost half of all hypertension cases. The increased sympathetic nervous system activity mediated by angiotensin II in the brain is known to be the main cause of NH. However, traditional antihypertensive drugs lack the ability to effectively penetrate the blood-brain barrier, making it challenging to manage NH. Therefore, developing strategies for targeted delivery of antihypertensives to the brain is crucial.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Paras Mani Giri, Anurag Banerjee, Buddhadev Layek
Summary: The advancement of nanotechnology has offered new possibilities for cancer management. Despite challenges in scaling up from the laboratory to clinical settings, nanomedicine shows great promise in tumor-specific delivery and minimizing side effects. This review discusses various types of nanoparticles, their role in cancer treatment, and highlights approved and ongoing clinical trials of nanoformulations for cancer therapy.
Article
Biochemistry & Molecular Biology
Shen Cheng, Zhiyuan Bo, Paul Hollenberg, Yoichi Osawa, Haoming Zhang
Summary: The study reports the use of amphipols to form a fully functional, soluble, homogeneous preparation of full-length CYP:POR complexes for biochemical and structural study. The stability and structure of the complex were validated using negative stain electron microscopy, revealing a tetrameric complex embedded in one amphipol nanoparticle. This represents the first report of the full-length mammalian CYP:POR complex by transmission EM and highlights the potential use of amphipols in biochemical and structural studies of functional CYP complexes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)