Article
Physics, Applied
Meng Shao, Rui Liu, Changxu Li, Zhe Chai, Zhensheng Zhong, Fengya Lu, Xunbin Wei, Jinhua Zhou, Min-Cheng Zhong
Summary: Optical tweezers are useful tools for non-contact cell manipulation, but currently limited to light-permeable organs. This study demonstrates the use of optical tweezers combined with oblique back-illumination microscopy to trap and evaluate erythrocyte deformability in opaque tissues.
APPLIED PHYSICS LETTERS
(2023)
Article
Cell Biology
Adam Varga, Adam Attila Matrai, Barbara Barath, Adam Deak, Laszlo Horvath, Norbert Nemeth
Summary: Plasma and blood osmolality values vary among different species and are closely regulated. These factors also affect microrheological parameters, such as RBC deformability and aggregation. This study explored the interspecies differences in RBC deformability at various blood osmolality levels and found significant variation among species. The findings highlight the importance of osmotic gradient deformability in understanding microrheological diversity and alterations caused by osmolality changes.
Article
Biochemistry & Molecular Biology
Alkmini T. Anastasiadi, Efthymios C. Paronis, Vasiliki-Zoi Arvaniti, Athanasios D. Velentzas, Anastasia C. Apostolidou, Evangelos G. Balafas, Monika Dzieciatkowska, Nikolaos G. Kostomitsopoulos, Konstantinos Stamoulis, Issidora S. Papassideri, Angelo D'Alessandro, Anastasios G. Kriebardis, Marianna H. Antonelou, Vassilis L. Tzounakas
Summary: Blood donors with beta-thalassemia trait (beta Thal(+)) have shown to have good storage capabilities for red blood cells, which are resistant to lysis and oxidative/proteotoxic stress. These RBCs perform better post-storage and demonstrate higher recovery rates in animal models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Travis Nemkov, Sarah C. Skinner, Elie Nader, Davide Stefanoni, Melanie Robert, Francesca Cendali, Emeric Stauffer, Agnes Cibiel, Camille Boisson, Philippe Connes, Angelo D'Alessandro
Summary: A controlled, high-intensity cycling test resulted in decreased RBC deformability, increased microparticle generation, oxidative stress, and activation of membrane lipid remodeling mechanisms. Metabolites involved in CoA synthesis and carnitine conversion were significantly correlated with RBC properties during exercise.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Physiology
Tamas Alexy, Jon Detterich, Philippe Connes, Kalman Toth, Elie Nader, Peter Kenyeres, Jose Arriola-Montenegro, Pinar Ulker, Michael J. Simmonds
Summary: This article introduces the knowledge of hemorheology, including the physical properties influencing blood flow and the role of hemorheology in health and disease. It also discusses the application of hemorheology in diseases and mechanical circulatory support devices, providing readers with a comprehensive overview.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Physiology
Felix Reichel, Martin Kraeter, Kevin Peikert, Hannes Glass, Philipp Rosendahl, Maik Herbig, Alejandro Rivera Prieto, Alexander Kihm, Giel Bosman, Lars Kaestner, Andreas Hermann, Jochen Guck
Summary: This study demonstrates for the first time an impairment of leukocyte properties in Chorea-Acanthocytosis (ChAc) patients. During treatment with dasatinib or lithium, alterations in red blood cell deformability and an increase in leukocyte stiffness were observed. These findings highlight the need for a systematic assessment of the contribution of impaired blood cell mechanics to the clinical manifestation of ChAc.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Materials Science, Biomaterials
Yan Wang, Shuhui Gao, Kongying Zhu, Lixia Ren, Xiaoyan Yuan
Summary: A feasible protocol for glycerol-free cryopreservation of human red blood cells using synthesized trehalose lipids and dissociative trehalose has been developed, achieving high cryosurvival rate and cell stability.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemical Research Methods
Yesaswini Komaragiri, Muzaffar H. Panhwar, Bob Fregin, Gayatri Jagirdar, Carmen Wolke, Stefanie Spiegler, Oliver Otto
Summary: This study introduces a new approach to determine the mechanical properties of individual mitochondria in a high-throughput manner and investigates the mechanical alterations of mitochondria under oxidative stress conditions.
Review
Mechanics
A. A. Boryaev
Summary: This paper discusses the need for precise position control in SSV tasks with microengines equipped with micronozzles, and introduces a method for calculating the boundary layer in micronozzles. By exploring methods for aerodynamic calculations and conducting experiments to verify the results, a mathematical model for solving the flow rate coefficient issue in micronozzles was established.
INTERNATIONAL JOURNAL OF NON-LINEAR MECHANICS
(2021)
Article
Chemistry, Multidisciplinary
Tamir Tsohar, Shaul Beyth, Alexander Gural, Dan Arbell, Saul Yedgar, Gregory Barshtein
Summary: This study investigated the impact of non-physiological mechanical stress during surgery on red blood cell deformability. The findings showed that mechanical stress reduces cell deformability, increases the percentage of undeformable cells, and leads to hemolysis of rigid cells. The final outcome depends on the initial concentration of undeformable cells in the sample.
APPLIED SCIENCES-BASEL
(2022)
Article
Computer Science, Information Systems
Muhammad Shahzad, Arif Iqbal Umar, Syed Hamad Shirazi, Israr Ahmed Shaikh
Summary: The study proposed a multi-level convolutional neural network model that preserves pixel-level semantic information for accurate quantification and morphological analysis of blood images, providing insights for the diagnosis and analysis of anemia.
Article
Medicine, General & Internal
Julie Vanderelst, Alexandre Rousseau, Nicolas Selvais, Patrick Biston, Karim Zouaoui Boudjeltia, Michael Piagnerelli
Summary: The study found that during sepsis, there are changes in the shape and deformability of red blood cells, as well as a decrease in the expression of the CD35 membrane protein. These changes may contribute to the phagocytosis of red blood cells and the development of anemia in sepsis.
FRONTIERS IN MEDICINE
(2022)
Article
Physiology
Michael Piagnerelli, Julie Vanderelst, Alexandre Rousseau, Daniel Monteyne, David Perez-Morga, Patrick Biston, Karim Zouaoui Boudjeltia
Summary: In patients with severe hypoxemic COVID-19, red blood cells (RBCs) showed normal deformability on admission, which remained unchanged over the first week despite changes in shape. This maintenance of normal RBC deformability may contribute to the adequate microcirculatory response to severe hypoxia observed in these patients.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Pablo Oyarzun, Eduardo Gallardo-Toledo, Javier Morales, Francisco Arriagada
Summary: This review compares transfersomes with other nanosystems, discusses their potential in topical treatment, and emphasizes the need for further research to demonstrate their significant clinical benefits compared with conventional therapies.
Article
Optics
Asa Asadollahbaik, Aashutosh Kumar, Michael Heymann, Harald Giessen, Jochen Fick
Summary: Dual-fiber optical tweezers based on nano-imprinted Fresnel lenses were used to study the vitality property of Dunaliella salina algae. Different forms of cyclic motion of living algae inside the optical trap were observed and analyzed. The technique has the potential to be applied in other areas, such as sperm analysis for fertility tests.
Article
Engineering, Biomedical
Vera Faustino, Diana Pinho, Susana O. Catarino, Graca Minas, Rui A. Lima
Summary: Efficient separation of blood components using microfluidic systems is crucial for disease detection and diagnosis. This study presents a novel multi-step microfluidic device based on passive crossflow filters, which allows simultaneous blood cell separation and cell deformability measurements. By evaluating and comparing different designs, the most effective configuration for the desired application was identified, showing the potential for highly efficient cell separation.
BIOMEDICAL MICRODEVICES
(2022)
Article
Mechanics
A. Rubio, E. J. Vega, A. M. Ganan-Calvo, J. M. Montanero
Summary: We experimentally studied the stability of micrometer weakly viscoelastic jets produced with transonic flow focusing. We found that highly stable jets are formed when adding low molecular weight polymer to water at low concentration and reducing the injected flow rate. The capillary instability is delayed and jet breakup occurs at distant locations from the ejected. The intense converging extensional flow in the ejection point results in long-lasting viscoelastic stress that is not relaxed in the jet even for times longer than the polymer relaxation time. The drag force exerted by the outer gas stream and partial polymer entanglement at the jet emission point may contribute to this effect.
Article
Polymer Science
Alejandro Rubio, Marta Lopez, Emilio J. Vega, Maria G. Cabezas
Summary: Fire-shaped nozzles are used to study deformability of microcapsules, particles, or cells in flow. The strain rate peak and time to neck can be easily estimated from flow rate and three geometrical parameters. The convergent region of the nozzles allows observation of deformation evolution.
Review
Chemistry, Multidisciplinary
Reinaldo R. Souza, Vera Faustino, Ines M. Goncalves, Ana S. Moita, Manuel Banobre-Lopez, Rui Lima
Summary: This review presents and analyzes the main traditional and new techniques used to measure the thermal conductivity of nanofluids. The fundamental parameters that affect the measurements are discussed, and a comparison is made between experimental and theoretical methods. The review aims to provide guidance for researchers interested in increasing the reliability of equipment used to measure the thermal conductivity of nanofluids.
Article
Physics, Fluids & Plasmas
A. Rubio, F. J. Galindo-Rosales, E. J. Vega, J. M. Montanero, M. G. Cabezas
Summary: This paper investigates the viscoelastic transition in transonic flow focusing when polymers of low molecular weights are dissolved in water at the appropriate concentration. The phenomenon is explained through the coil-stretch transition of polymers crossing the tapering meniscus, where high strain rates are produced by the airstream. The resulting elastic stress stabilizes the flow, leading to thinner and longer jets compared to Newtonian fluids, which has important implications at the technological level.
PHYSICAL REVIEW FLUIDS
(2022)
Article
Materials Science, Textiles
Alejandro Rubio, Francisco Javier Martinez-Vazquez, Maria Guadalupe Cabezas, Jose Maria Montanero, Emilio Jose Vega
Summary: In this study, we investigated the production of fibers using Solution Blow Spinning (SBS) technique under low polymer molecular weight and air-coflow pressure conditions. We found that the fiber diameter decreases with decreasing coflow temperature. The coflowing airstream causes whipping oscillations of the viscoelastic jet, with larger amplitude observed in the non-protruding spinneret position. However, the spinneret position does not significantly affect the diameter of the jet and fiber, thus recommending the use of the protruding configuration.
FIBERS AND POLYMERS
(2022)
Article
Thermodynamics
A. Rubio, B. N. Munoz-Sanchez, M. G. Cabezas, J. M. Montanero
Summary: This study measures the minimum liquid flow rate Q(min) required for steady jetting in gaseous flow focusing through orifices as small as 30 µm in diameter. The results obtained for different diameters and liquids are compared, and the influence of gas viscosity on the stability limit is determined using dimensional analysis. For low and moderately low-viscosity liquids, there is an optimum gas viscosity value at which Q(min) reaches its minimum, resulting in the minimum jet diameter in front of the discharge orifice.
EXPERIMENTAL THERMAL AND FLUID SCIENCE
(2023)
Article
Engineering, Chemical
Ines M. Goncalves, Ines Castro, Filipe Barbosa, Vera Faustino, Susana O. Catarino, Ana Moita, Joao M. Miranda, Graca Minas, Patricia C. Sousa, Rui Lima
Summary: The separation capacity of a multi-step crossflow microfluidic device was evaluated by using a blood analogue fluid and human red blood cells. The results showed that the device effectively separated the cells and the blood analogue fluid exhibited similar flow behavior to real blood, providing important data support for related research.
Article
Chemistry, Physical
A. Rubio, M. Lopez, T. Rodrigues, L. Campo-Deano, E. J. Vega
Summary: There has been significant interest in recent years in producing fluids that have rheological properties similar to real blood. These fluids can be used in both microscale biomicrofluidics and macroscale forensic science. The inclusion of flexible microparticles in blood analogue fluids is essential to replicate the behavior of blood flow in these fields. This study describes a protocol for producing a whole human blood analogue composed of a proposed plasma analogue and flexible spherical microparticles that mimic the key structural attributes of red blood cells (RBCs). The study also explores the flow of the proposed fluid through a microfluidic channel and its impact on a solid surface, demonstrating the in vitro reproduction of multiphase flow effects and its application in bloodstain pattern analysis in forensic science.
Article
Engineering, Electrical & Electronic
Diana Pinho, Vera Faustino, Susana O. Catarino, Ana I. Pereira, Graca Minas, Fernando T. Pinho
Summary: This paper presents a multi-step microfluidic device that is able to separate blood cells and measure their deformability. The device found that white blood cells have lower deformability than red blood cells, and pathological red blood cells have lower deformability than healthy ones. In addition, blood from patients with type II diabetes has higher viscosity. This device shows potential as a supplementary tool in clinical diagnosis and biological research.
MICRO AND NANO ENGINEERING
(2022)