Review
Allergy
F. L. de Boer, H. F. van Der Molen, S. Kezic
Summary: Dysfunction of the skin barrier is crucial in inflammatory skin diseases, and epidermal biomarkers can aid in evaluating its functionality. This review provides an overview of recent studies on epidermal biomarkers associated with the skin barrier's function and integrity and their application in research on atopic dermatitis and contact dermatitis. Potential biomarkers include lipids, natural moisturizing factors, structural proteins, corneocyte surface topography, and elasticity. While these biomarkers show promise, their validation for specific diseases is still needed.
CONTACT DERMATITIS
(2023)
Article
Dermatology
Mads Almose Ropke, Anna Mekulova, Christian Pipper, Maigi Eisen, Kristi Pender, Pieter Spee, Sanja Kezic
Summary: This study evaluated the use of the non-invasive patch technique by FibroTx for skin biomarker analysis in AD patients. The results demonstrated that this method can detect key biomarkers on lesional and non-lesional skin, which can reflect changes based on treatment. These findings support the effectiveness of using patch sampling for non-invasive assessment of inflammatory processes in AD skin.
SKIN RESEARCH AND TECHNOLOGY
(2021)
Article
Medicine, General & Internal
Ayano Maruyama, Risa Tamagawa-Mineoka, Hiromi Nishigaki, Koji Masuda, Norito Katoh
Summary: This is an important study exploring potential local and systemic biomarkers of AD, which will have clinical significance in discovering and assessing new biomarkers for AD.
Article
Dermatology
Masataka Ota, Takashi Sasaki, Tamotsu Ebihara, Emiko Yokosawa, Yumi Murakami, Hiroshi Matsunaka, Yuko Chinuki, Masayuki Amagai, Eishin Morita
Summary: The study found that loss-of-function mutations of the filaggrin (FLG) gene do not affect the severity of atopic dermatitis (AD) or skin barrier function, and have little impact on the amount of FLG protein and total amino acids in the stratum corneum of lesional skin. The results suggest that the production of FLG in skin lesions is influenced by the activation of Th2-dominant inflammatory cells along with FLG abnormalities.
JOURNAL OF DERMATOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Adrienn Jenei, Gergo Kallo, Zsolt Dajnoki, Krisztian Gaspar, Andrea Szegedi, Aniko Kapitany, Eva Csosz
Summary: This study developed a novel approach for examining AMPs in the outer layer of the epidermis, with hexane-free processing producing higher protein yield. Among the investigated peptides, 9 could be detected in healthy or inflamed skin, and significant topographical differences were observed in the amount of certain peptides among different regions of healthy skin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Sagar Shukla, Annette L. Bunge, Hazem E. Hassan, Audra L. Stinchcomb
Summary: The study evaluated the impact of different investigators in studies using a tape stripping protocol to minimize investigator variability. The results showed that the measurements of different investigators are reproducible within the limitations of measurement variability, which can be managed by increasing volunteer numbers.
PHARMACEUTICAL RESEARCH
(2022)
Article
Biology
Trinidad Montero-Vilchez, Carlos Cuenca-Barrales, Juan-Angel Rodriguez-Pozo, Pablo Diaz-Calvillo, Jesus Tercedor-Sanchez, Antonio Martinez-Lopez, Alejandro Molina-Leyva, Salvador Arias-Santiago
Summary: This study investigated the changes in skin barrier function with age in patients with atopic dermatitis (AD). The results showed that as age increased, AD patients had higher transepidermal water loss (TEWL), lower stratum corneum hydration (SCH), and reduced elasticity compared to healthy individuals. There was a negative correlation between age and elasticity, which was stronger in AD patients than in controls. Linear regression analyses revealed that age and disease severity had a significant impact on elasticity in AD patients. These findings suggest that age plays a role in impairing skin barrier function in AD patients, particularly in terms of elasticity.
Article
Pharmacology & Pharmacy
Mihaela Gorcea, Majella E. Lane, David J. Moore
Summary: The purpose of this in vivo exploratory study was to investigate the conformational order and chain packing of human stratum corneum lipids in healthy face skin as a function of depth. The study found that the lipid order of the stratum corneum increases with depth in face skin, but shows significant differences compared to arm skin.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Konstantin Simon, Gila Oberender, Alexander Roloff
Summary: Tape stripping is a common method used in studies on drug penetration through the skin, but the amount of stratum corneum removed by each tape strip is still under debate. Some studies suggest that the amount of stratum corneum decreases with increasing depth, while others find a constant removal rate.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Allergy
Susumu Murata, Sakae Kaneko, Eishin Morita
Summary: The improvement of dermatitis in atopic dermatitis (AD) patients is correlated with a decrease in stratum corneum interleukin-8 (scIL-8) levels after topical corticosteroid treatment. The reduction in scIL-8 levels is associated with the improvement in local skin severity in AD, indicating that scIL-8 may be a suitable biomarker for monitoring therapeutic effects in AD patients.
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
(2021)
Review
Immunology
Risa Tamagawa-Mineoka
Summary: The skin acts as a physical barrier and toll-like receptors (TLRs) play a role in both infectious and non-infectious inflammatory diseases. In atopic dermatitis (AD), skin barrier dysfunction allows stimuli to penetrate the skin and activate the innate immune system, leading to inflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Allergy
Evgeny Berdyshev, Jihyun Kim, Byung Eui Kim, Elena Goleva, Taras Lyubchenko, Irina Bronova, Anna Sofia Bronoff, Olivia Xiao, Jiwon Kim, Sukyung Kim, Mijeong Kwon, Sungjoo Lee, Yu Jeong Seo, Kyunga Kim, Suk-Joo Choi, Soo-Young Oh, Seung Hwan Kim, So Yeon Yu, Seung Yong Hwang, Kangmo Ahn, Donald Y. M. Leung
Summary: A study found that the risk of developing atopic dermatitis in the future can be predicted by analyzing the skin surface of infants at the age of two months, and identified potential biomarkers.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Dermatology
Julie Solberg, Stine B. Jacobsen, Jeppe D. Andersen, Thomas Litman, Nina H. Ulrich, Malin G. Ahlstroem, Marie-Louise Kampmann, Niels Morling, Jacob P. Thyssen, Jeanne D. Johansen
Summary: The study demonstrates that the stratum corneum transcriptome in AD skin can be assessed by tape stripping, potentially playing a central role in future skin biomarker research.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Tu Hu, Tanja Todberg, Daniel Andersen, Niels Banhos Danneskiold-Samsoe, Sofie Boesgaard Neestrup Hansen, Karsten Kristiansen, David Adrian Ewald, Susanne Brix, Joel Correa da Rosa, Ilka Hoof, Lone Skov, Thomas Litman
Summary: This study used BRB-seq technique to analyze the transcriptome of tape-stripped skin samples from atopic dermatitis patients and healthy controls. An AD signature consisting of genes related to skin barrier and inflammatory response was identified. However, the low and highly variable transcript counts in tape-stripped samples were a limiting factor for epidermal transcriptome profiling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Eleonora Maretti, Eliana Leo, Cecilia Rustichelli, Eleonora Truzzi, Cristina Siligardi, Valentina Iannuccelli
Summary: This study investigated the use of Nanostructured Lipid Carriers (NLC) to enhance skin permeation of the highly lipophilic beta-carotene (BC) for improved antioxidant effects. The smallest sized NLC sample showed deeper penetration into the skin barrier, potentially providing better protection against oxidative damages.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Allergy
Nathan Dyjack, Elena Goleva, Cydney Rios, Byung Eui Kim, Lianghua Bin, Patricia Taylor, Caroline Bronchick, Clifton F. Hall, Brittany N. Richers, Max A. Seibold, Donald Y. M. Leung
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2018)
Article
Materials Science, Multidisciplinary
Yong Yan, Shuo Qian, Ben Garrison, Tyler Smith, Peter Kim
JOURNAL OF NUCLEAR MATERIALS
(2018)
Article
Allergy
Elizabeth Huiwen Tham, Nathan Dyjack, Byung Eui Kim, Cydney Rios, Max A. Seibold, Donald Y. M. Leung, Elena Goleva
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2019)
Article
Allergy
Jihyun Kim, Byung Eui Kim, Donald Y. M. Leung
ALLERGY AND ASTHMA PROCEEDINGS
(2019)
Article
Allergy
Minyoung Jung, Insung Kim, Ji Young Lee, Hyun Mi Kim, Mijeong Kwon, Minjeong Kim, Kyung-Min Lim, Peter S. Kim, Kangmo Ahn, Jihyun Kim
ALLERGOLOGY INTERNATIONAL
(2020)
Review
Immunology
Kangmo Ahn, Byung Eui Kim, Jihyun Kim, Donald Y. M. Leung
CURRENT OPINION IN IMMUNOLOGY
(2020)
Article
Medicine, Research & Experimental
Byung Eui Kim, Jihyun Kim, Elena Goleva, Evgeny Berdyshev, Jinyoung Lee, Kathryn A. Vang, Un Ha Lee, SongYi Han, Susan Leung, Clifton F. Hall, Na-Rae Kim, Irina Bronova, Eu Jin Lee, Hye-Ran Yang, Donald Y. M. Leung, Kangmo Ahn
Summary: The study revealed that PM2.5 can inhibit FLG and other proteins in the stratum corneum, compromising skin barrier function and increasing skin permeability, potentially contributing to the development and exacerbation of allergic diseases.
Article
Allergy
Byung Eui Kim, Jessica Hui-Beckman, Taras Lyubchenko, Clifton F. Hall, Sahand Fallahi, Amelia Brull, Elena Goleva, Donald Y. M. Leung
Summary: Exposure to low temperature can cause skin barrier dysfunction mediated by TRPV1, leading to an increased risk of atopic dermatitis and food allergy.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Review
Allergy
Jessica Hui-Beckman, Byung Eui Kim, Donald Y. M. Leung
Summary: As the incidence of allergies continues to rise, understanding the origin of allergy has become important. The perinatal environment plays a crucial role in this understanding, as maternal exposure significantly affects fetal health. Dysfunctional epithelial barriers also play a key role in the development of allergies.
ALLERGY ASTHMA & IMMUNOLOGY RESEARCH
(2022)
Article
Allergy
Jihyun Kim, Byung Eui Kim, Evgeny Berdyshev, Irina Bronova, Lianghua Bin, Jaewoong Bae, Seokjin Kim, Hye-Young Kim, Un Ha Lee, Myoung Shin Kim, Hyunmi Kim, Jinyoung Lee, Clifton F. Hall, Jessica Hui-Beckman, Yunhee Chang, Anna Sofia Bronoff, Dasom Hwang, Hae-Young Lee, Elena Goleva, Kangmo Ahn, Donald Y. M. Leung
Summary: This study found that Staphylococcus aureus infection can cause skin barrier disruption in atopic dermatitis patients. Moreover, the study also found that S. aureus induces aberrant epidermal lipid composition and skin barrier dysfunction by inhibiting fatty acid elongase enzymes in epidermal cells.
Article
Allergy
Jihyun Kim, Byung Eui Kim, Elena Goleva, Evgeny Berdyshev, Jaewoong Bae, Seokjin Kim, Hye-young Kim, Una Ha Lee, Myoung Shin Kim, Minyoung Jung, Hyunmi Kim, Jinyoung Lee, Donald Y. M. Leung, Kangmo Ahn
Summary: This study aimed to investigate the epidermal lipid profiles and their association with skin microbiome compositions in children with atopic dermatitis (AD). Skin tape stripping was used to obtain specimens from AD children and healthy subjects. The results showed that AD lesional skin had increased levels of certain ceramides, sphingomyelin, and lysophosphatidylcholine compared to nonlesional skin and control subjects. The lipid ratio and the proportions of certain bacteria were found to be associated with skin barrier dysfunction. These findings suggest that aberrant lipid profiles in pediatric AD skin are linked to microbial dysbiosis and cutaneous barrier dysfunction.
ALLERGY ASTHMA & IMMUNOLOGY RESEARCH
(2023)
Article
Immunology
Bo Ra Lee, Hye-In Jung, Su Kyung Kim, Mijeong Kwon, Hyunmi Kim, Minyoung Jung, Yechan Kyung, Byung Eui Kim, Suk-Joo Choi, Soo-Young Oh, Sun-Young Baek, Seonwoo Kim, Jaewoong Bae, Kangmo Ahn, Jihyun Kim
Summary: Dietary diversity is associated with a reduced risk of developing egg allergy in high-risk infants and can increase gut microbial diversity. Furthermore, low dietary diversity is associated with increased gene expression of cytokines.
Review
Allergy
Jihyun Kim, Byung Eui Kim, Kangmo Ahn, Donald Y. M. Leung
ALLERGY ASTHMA & IMMUNOLOGY RESEARCH
(2019)