Article
Biochemistry & Molecular Biology
Chengkun Liu, Chang Liu, Zhiyuan Liu, Zhuang Shi, Shihai Liu, Xiaojuan Wang, Xiaoqiang Wang, Fang Huang
Summary: A thermogelling chitosan/glycerophosphate formulation was developed by incorporating dihydrocaffeic acid (DHCA), which significantly enhanced gel-forming capacity and tissue adherence. The thermosensitive hydrogels showed improved hemostasis and reduced blood loss in both in vitro and in vivo models. The results demonstrated the potential of enhancing thermogelling and bioadhesion of chitosan-based hydrogels through noncovalent supramolecular mechanisms.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Bo Seul Jang, Kyoung Hwan Park, Eun Yeong Suh, Byoung-Seok Lee, Sun-Woong Kang, Kang Moo Huh
Summary: Chemically crosslinked hydrogels prepared by photocrosslinking of methacrylated HGCs demonstrated enhanced gel stability and good spheroid-forming abilities for long-term 3D cell cultures. M-HGC-coated dishes effectively generated spheroids of larger size and higher cell density depending on the crosslinking density of the M-HGCs, showing potential for widely applied in tissue engineering and drug screening. The hydrogel-coated dish system provides an effective technique to produce cell spheroids with customized sizes and densities essential for various applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Applied
O. Catanzano, G. Gomez d'Ayala, A. D' Agostino, F. Di Lorenzo, C. Schiraldi, M. Malinconico, R. Lanzetta, F. Bonina, P. Laurienzo
Summary: Chitosan-based wound dressings loaded with Opuntia ficus-indica extract were prepared in this study. The crosslinking of chitosan with low molecular weight diepoxy-poly(ethylene glycol) was confirmed, and the films showed promising characteristics for skin injury repair applications. Scratch tests on keratinocytes monolayers demonstrated the significant enhancement of cell migration in the presence of OPU-loaded samples.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Applied
Yuwei Zhang, Wenxin Zhao, Zhenhao Lin, Zhongfeng Tang, Baofeng Lin
Summary: Strawberry waste caused by spoilage and decay is a pressing issue that needs to be addressed. Researchers have developed hydrogel films (HGF) that can effectively delay strawberry spoilage by forming a coating on the fruit's surface. The HGF exhibits excellent moisture permeability and antibacterial properties, keeping the strawberries fresh for extended periods at different temperatures. The regenerative HGF also shows promising performance, maintaining strawberry freshness even after several cycles of dissolution and regeneration.
CARBOHYDRATE POLYMERS
(2023)
Article
Polymer Science
Kyung Min Yoo, Sean Murphy, Aleksander Skardal
Summary: Hydrogels play a significant role in regenerative medicine and tissue engineering, with versatile properties suitable for various clinical applications, including cell encapsulation, drug delivery, and biofabrication. This study describes a well-characterized, defined extracellular matrix-based hydrogel platform with versatile properties.
Article
Energy & Fuels
Hyocheol Lee, Anjali Nagapadi Preman, Thuan Ngoc Vo, Jin-Hyeong Lee, Il Tae Kim, Suk-kyun Ahn
Summary: In order to overcome the rapid capacity decay of silicon as a battery material, researchers have synthesized a series of multifunctional acrylic random copolymer binders and modulated their adhesive and mechanical properties. By adding acrylic acid and glyceryl groups, these binders can undergo in situ crosslinking at lower temperatures, leading to improved electrochemical performance.
INTERNATIONAL JOURNAL OF ENERGY RESEARCH
(2022)
Review
Engineering, Biomedical
Quanjing Mei, Jingdong Rao, Ho Pan Bei, Yaxiong Liu, Xin Zhao
Summary: Three-dimensional (3D) bioprinting is a promising strategy for bone manufacturing, with control over geometry and microarchitectures of the scaffolds. Photo-crosslinkable hydrogel is a promising material in tissue engineering, with biocompatibility, easy fabrication, and controllable properties for bone and cartilage scaffolds. This review discusses the applications of 3D bioprinted photo-crosslinkable hydrogel scaffolds for bone and cartilage engineering and future perspectives in the field.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2021)
Article
Engineering, Environmental
Hogyun Cheong, Young-Joon Jun, Eun Young Jeon, Jong In Lee, Hyun Jun Jo, Hae Yeon Park, Eunjin Kim, Jong Won Rhie, Kye Il Joo, Hyung Joon Cha
Summary: A method of seamless nerve anastomosis using a bioadhesive hydrogel was proposed in this study, which effectively promoted nerve regeneration by inducing macrophage polarization, showing significant advantages over traditional suture techniques.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Physical
Aida Sadeghian, Mahshid Kharaziha, Maryam Khoroushi
Summary: A novel nanocomposite hydrogel was designed with osteoconductivity and high strength, reinforced with bioactive glass to enhance mechanical properties and bioactivity, significantly promoting cell attachment, proliferation, and alkaline phosphatase activity.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Nanoscience & Nanotechnology
Ruosen Xie, Yu-Chung Chen, Yi Zhao, Nisakorn Yodsanit, Yuyuan Wang, Naoaki Yamamoto, Dai Yamanouchi, Shaoqin Gong
Summary: The injectable hydrogel reported in this study undergoes in situ covalent crosslinking to form a dual-crosslinking network (DCN) hydrogel, which provides a safe, consistent, and controlled embolization. It is mechanically stable, visible under X-ray imaging, and capable of achieving immediate embolization with long-term occlusion in vivo tests. The DCN hydrogel offers a viable, biocompatible, and cost-effective alternative to current embolic materials for endovascular embolization.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Min A. Gwak, Su Jin Lee, Dongjin Lee, Su A. Park, Won Ho Park
Summary: A novel self-crosslinkable bioink derived from chitosan and gallic acid is developed, which possesses excellent printability, gelation, mechanical properties, and cytocompatibility. The CS-GA hydrogel can rapidly form at physiological pH without chemical crosslinking agents. The CS-GA hydrogel exhibits various physical and chemical interactions, fast gelation rates, and excellent mechanical properties (>337 kPa), and significantly improves cell viability and proliferation. This self-crosslinkable bioink has the potential to overcome the limitations of conventional bioinks.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Pharmacology & Pharmacy
Hillary Mndlovu, Pradeep Kumar, Lisa C. du Toit, Yahya E. Choonara
Summary: Cytocompatibility, biocompatibility, and biodegradability are desirable qualities of wound dressings. A three-stepped approach was used to fabricate a particulate, partially crosslinked, and transferulic acid-loaded chitosan-alginate interpolymer complex with enhanced wound healing capabilities.
Article
Polymer Science
Tomoki Maeda, Keishi Tanimoto, Atsushi Hotta
Summary: Thermoresponsive gelation of nanocomposites containing PLGA-b-PEG-b-PLGA triblock copolymers and LAPONITE was studied by decreasing PLGA molecular weights, revealing that lower PLGA molecular weights led to more stable gelation temperatures and less dependence on copolymer concentration. An almost linear decrease in gelation temperature with increasing LAPONITE concentration was observed, making the nanocomposite suitable for biomedical applications.
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Filippos F. Karageorgos, Costas Kiparissides
Summary: The present study focuses on the mathematical modeling of the crosslinking kinetics of polymer-phenol conjugates mediated by the HRP-H2O2 initiation system. A dynamic MC kinetic model is developed to quantify the effects of crosslinking conditions on gelation onset time, molecular weight distribution, and molecular weights of polymer chains. The model is successfully validated through experimental measurements, allowing for the calculation of hydrogel properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Felipe Lopez-Saucedo, Leticia Buendia-Gonzalez, Hector Magana, Guadalupe Gabriel Flores-Rojas, Emilio Bucio
Summary: This study proposes the development of an affordable polymer film for healthcare purposes. The film consists of chitosan, itaconic acid, and Randia capitata fruit extract. The film is formed through crosslinking of chitosan with itaconic acid and in situ addition of R. capitata fruit extract. The resulting ionically crosslinked composite film shows promising potential as a wound dressing due to the combined properties of chitosan and R. capitata fruit extract in supporting epithelial regeneration.
Article
Engineering, Biomedical
Seungho Lim, Hong Yeol Yoon, Soon-Jung Park, Sukyung Song, Man Kyu Shim, Suah Yang, Sun-Woong Kang, Dong-Kwon Lim, Byung-Soo Kim, Sung-Hwan Moon, Kwangmeyung Kim
Summary: A bioorthogonal labeling strategy was developed to non-invasively track human embryonic stem cell-derived endothelial progenitor cells (hEPCs) after transplantation, showing strong NIRF signals in vivo and potential therapeutic applications for hind limb ischemia.
Article
Agriculture, Multidisciplinary
Mohammad Zahirul Islam, Buem-Jun Park, Su-Yeon Jeong, Sun-Woong Kang, Bo-Kyung Shin, Young-Tack Lee
Summary: Drought stress increased the levels of biochemical compounds in barley grass and wheatgrass extracts, with wheatgrass showing higher phenolics, flavonoids, and vitamin C contents. Additionally, wheatgrass extracts exhibited significantly higher antioxidant enzyme activities compared to barley grass extracts under severe drought stress.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Oncology
Soon-Jung Park, Hyeok Kim, Sunghun Lee, Jongsoo Kim, Taek-Hee Jung, Seong Woo Choi, Bong-Woo Park, Sun-Woong Kang, David A. Elliott, Edouard G. Stanley, Andrew G. Elefanty, Kiwon Ban, Hun-Jun Park, Sung-Hwan Moon
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Cell & Tissue Engineering
Woo Beom Ahn, Yu Bin Lee, Yi-Hwa Ji, Kyoung-Sik Moon, Hyon-Seok Jang, Sun-Woong Kang
Summary: This study developed a decellularized human adipose tissue matrix (DM) as an alternative graft material for bone defects. The DM showed the ability to form new bone tissue when implanted and had similar bone tissue regeneration efficiency compared to a commercial product. Therefore, this study provides an innovative solution to address the problem of insufficient graft materials.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Multidisciplinary Sciences
Hyosuk Kim, Byeong-Wook Song, Soon-Jung Park, Seong Woo Choi, Hanbyeol Moon, Ki-Chul Hwang, Sun-Woong Kang, Sung-Hwan Moon, Yoosoo Yang, Ick Chan Kwon, Sun Hwa Kim
Summary: This study proposes an extracellular vesicle (EV)-guided, nonviral, direct lineage conversion strategy to enhance the transdifferentiation of fibroblasts to induced cardiomyocyte-like cells (iCMs). The resulting iCMs show typical cardiac features and have gene expression profiles similar to cardiomyocytes. This method achieves a high conversion efficiency (>60%) and establishes a more accessible platform for generating specialized somatic cells through direct lineage conversion.
Article
Chemistry, Multidisciplinary
Da-Eun Kim, Yu Bin Lee, Hye-Eun Shim, Jin Jung Song, Ji-Seok Han, Kyoung-Sik Moon, Kang Moo Huh, Sun-Woong Kang
Summary: This study developed a non-cell adhesive polymer HGC to overcome the limitations of cell adhesion in three-dimensional culture, ensuring uniform cell distribution and maintaining the shape and size of spheroids.
Article
Chemistry, Applied
Ji-Eun Jeong, Sang-Soo Han, Hye-Eun Shim, Woojin Kim, Byoung-Seok Lee, Yong- Jin Kim, Sun-Woong Kang
Summary: This study proposes a new technique for generating spheroids using scaffold-based methods, which successfully produced uniform-sized spheroids with high viability and increased expression levels of proteins and genes required for cell-specific functions. These physiologically relevant models have potential applications in regenerative medicine and drug screening.
CARBOHYDRATE POLYMERS
(2022)
Article
Immunology
Rona Harari-Steinfeld, V. S. S. Abhinav Ayyadevara, Lizette Cuevas, Francesco Marincola, Kyung-Ho Roh
Summary: The horizon of immunotherapy using CAR-T cells is extending to treat solid tumors. Precise in-vitro evaluations of CAR-T cells for their phenotypes, quantity and quality of activation are critical. A novel biomaterial platform, aaTPs, has been developed to precisely control antigen densities and enable clean and standardized in-vitro assays. The aaTP platform has great potential for the development and evaluation of CAR-T products.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Thuy Trang Truong, Yu Bin Lee, Kyoung Hwan Park, Hye-Eun Shim, Jin Jung Song, Hyung-Sun Kim, Jeong Ho Hwang, Sun-Woong Kang, Kang Moo Huh
Summary: Three-dimensional (3D) spheroid culture is widely used, but time-consuming and unsuitable for urgent situations. Cryopreservation methods for spheroids are required for on-demand and ready-to-use applications. Short incubation periods were found to be suitable for the cryopreservation process, ensuring viability and migration of the spheroids. This study provides a chondrocyte-based ready-to-use spheroid system that can be applied to regenerative medicine and other cell types.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2023)
Article
Engineering, Environmental
Kyoung Hwan Park, Bokyeong Ryu, Jin Jung Song, Hye-Eun Shim, Sun-Sook Song, Ji-Seok Han, C-Yoon Kim, Kang Moo Huh, Sun-Woong Kang
Summary: This study presents a new method for spheroid formation by incorporating hyaluronic acid (HA) microparticles in the culture. Spheroids containing HA microparticles exhibited enhanced cell migration activity, higher survival rate, and increased albumin secretion ability after transplantation in the liver of mice, compared to plain spheroids. This 3D cell culture system with HA microparticles shows promise in improving engraftment and efficiency of cell therapy for regenerative medicine.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
So-Jung Gwak, Yu Bin Lee, Eun Joo Lee, Kyoung Hwan Park, Sun-Woong Kang, Kang Moo Huh
Summary: The market for injectable dermal fillers is booming due to growing interest in appearance management. However, the durability and retention time of hyaluronic acid (HA) hydrogels, commonly used as fillers, are limited. This study introduces acetylated hyaluronic acid-divinyl sulfone (AcHA-DVS) hydrogels as a promising solution to improve the physical properties and gel retention time of HA fillers.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2023)
Article
Polymer Science
Eun Young Koo, Kyoung Hwan Park, Sun-Woong Kang, Kang Moo Huh
Summary: In this study, effective 3D cell culture dishes were designed by optimizing the structure and coating stability of hexanoyl glycol chitosan (HGC) materials. Different HGCs with varying substitution ratios were synthesized and compared for their coating stability and 3D cell culture ability. Mesh-pattern culture dishes were also produced for mass-production of uniform-sized cell spheroids. HGC polymer with a substitution ratio of 36% or more showed high stability and spheroid formation ability, and the mesh-pattern culture dishes were effective for mass-production of uniform and controlled sizes.
Article
Agriculture, Multidisciplinary
Mohammad Zahirul Islam, Sun-Woong Kang, Na-Gyeong Koo, Ye-Jin Kim, Jae Kwang Kim, Young-Tack Lee
Summary: This study investigated the changes in bioactive compounds of Cassia tora seeds during germination. The results showed that the crude ash, crude fat, and crude protein content of the seeds slightly increased during the germination process. The study also found that the total carotenoid content, anthocyanin content, vitamin C content, phenolic content, and flavonoid content increased after germination, which subsequently increased the antioxidant activities.
COGENT FOOD & AGRICULTURE
(2023)
Article
Biochemistry & Molecular Biology
Jin-Ju Kim, Jae-Hyun Park, Hyeok Kim, Woo-Sup Sim, Seokbeom Hong, Yeon-Jik Choi, Hyo-Jin Kim, Soon Min Lee, Dongha Kim, Sun-woong Kang, Kiwon Ban, Hun-Jun Park
Summary: Researchers have developed a strategy using SDF1 alpha-engineered mesenchymal stem cells (SDF1 alpha-eMSCs) to simultaneously regenerate blood vessels and skeletal muscles in limbs experiencing ischemia. These cells can provide long-term paracrine effects, promoting vascular regeneration and skeletal muscle repair, and have potential as an effective therapeutic strategy for limb salvage in patients with critical limb ischemia.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Meeting Abstract
Cell & Tissue Engineering
Kyeong Eun Lee, Da Hyeon Choi, Sun-Woong Kang, Kang Moo Huh, Yoon Shin Park
TISSUE ENGINEERING PART A
(2022)