Article
Biotechnology & Applied Microbiology
Zhenyu Xu, Junjie Sun, Yong Mao, Yang Chen, Ting Zhang, Yan Qin, Dong Hua
Summary: The study revealed that Higd-1a is underexpressed in colon adenocarcinoma (COAD) cells and its overexpression impairs the proliferation, migration, and invasiveness of COAD cells.
Article
Oncology
Qunli Xiong, Ying Zhou, Su Zhang, Yaguang Zhang, Yongfeng Xu, Yang Yang, Congya Zhou, Zhu Zeng, Junhong Han, Qing Zhu
Summary: The expression of NSD family genes is correlated with clinical prognosis in multiple types of cancers. NSD3 is prominently expressed in pancreatic cancer and is significantly linked with clinical outcomes. Furthermore, NSD3 is frequently amplified, exhibits low promoter methylation, and is correlated with immune cell infiltration and enhanced proliferation of pancreatic cancer. Our experiments suggest that NSD3 may regulate gene expression and growth factor signaling in pancreatic cancer.
Review
Biochemistry & Molecular Biology
Matthew D. Gibbons, Yu Fang, Austin P. Spicola, Niko Linzer, Stephen M. Jones, Breanna R. Johnson, Lu Li, Mingyi Xie, Jorg Bungert
Summary: Enhancers in higher eukaryotes and upstream activating sequences (UASs) in yeast recruit components of the RNA polymerase II transcription machinery. Pol II recruited to enhancers in higher eukaryotes initiates transcription and generates enhancer RNA (eRNA), while UASs in yeast do not recruit the required transcription factor TFIIH. In both systems, Pol II is transferred from enhancers/UASs to promoters.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Xianghai Meng, Zhenjun Zhang, Lin Chen, Xi Wang, Qingguo Zhang, Shuheng Liu
Summary: The study found that TTN-AS1 expression was increased in OS while miR-16-1-3p expression was decreased. By mediating the miR-16-1-3p/TFAP4 axis, TTN-AS1 promoted proliferation, migration, invasion, and epithelial-mesenchymal transition of OS cells.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Keisuke Omori, Shohei Otani, Yuki Date, Tomoya Ueno, Tomoko Ito, Masahiro Umeda, Kosei Ito
Summary: Osteosarcoma is a disease in humans characterized by TP53 mutations. In mice, loss of p53 triggers OS development, and osteoprogenitor-specific p53-deleted mice are widely used to study the process of osteosarcomagenesis. However, the molecular mechanisms underlying the initiation or progression of OS following or parallel to p53 inactivation remain largely unknown. This study reveals a novel tumor suppressive mechanism mediated by C/ebp alpha in p53-deficient osteosarcomagenesis, highlighting the importance of the Runx-Myc oncogenic axis as a therapeutic target for OS.
Article
Multidisciplinary Sciences
Yunlong Cao, Ayijiang Yisimayi, Fanchong Jian, Weiliang Song, Tianhe Xiao, Lei Wang, Shuo Du, Jing Wang, Qianqian Li, Xiaosu Chen, Yuanling Yu, Peng Wang, Zhiying Zhang, Pulan Liu, Ran An, Xiaohua Hao, Yao Wang, Rui Feng, Haiyan Sun, Lijuan Zhao, Wen Zhang, Dong Zhao, Jiang Zheng, Lingling Yu, Can Li, Na Zhang, Rui Wang, Xiao Niu, Sijie Yang, Xuetao Song, Yangyang Chai, Ye Hu, Yansong Shi, Linlin Zheng, Zhiqiang Li, Qingqing Gu, Fei Shao, Weijin Huang, Ronghua Jin, Zhongyang Shen, Youchun Wang, Xiangxi Wang, Junyu Xiao, Xiaoliang Sunney Xie
Summary: Omicron sublineages BA.2.12.1, BA.4 and BA.5 have higher transmissibility and increased evasion of neutralizing antibodies compared to the BA.2 lineage. They exhibit similar binding affinities to the ACE2 receptor as BA.2. BA.1 infection after vaccination boosts humoral immune memory against wild-type SARS-CoV-2, but these antibodies are largely evaded by BA.2 and BA.4/BA.5 variants.
Article
Genetics & Heredity
Leandro M. Colli, Lea Jessop, Timothy A. Myers, Sabrina Y. Camp, Mitchell J. Machiela, Jiyeon Choi, Renato Cunha, Olusegun Onabajo, Grace C. Mills, Virginia Schmid, Seth A. Brodie, Olivier Delattre, David R. Mole, Mark P. Purdue, Kai Yu, Kevin M. Brown, Stephen J. Chanock
Summary: The DPF3 variant at the 14q24 locus plays a critical role in RCC susceptibility, with its overexpression increasing cell growth rates and affecting gene expression and chromatin accessibility, resulting in decreased apoptosis and activation of oncogenic pathways.
AMERICAN JOURNAL OF HUMAN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Xiao Li, Xiaona Tang, Xinyang Bing, Christopher Catalano, Taibo Li, Gabriel Dolsten, Carl Wu, Michael Levine
Summary: The impact of genome organization on gene expression control is a major challenge in regulatory biology. Previous research has focused on CTCF-enriched boundary elements and TADs, which enable long-range DNA associations. However, there is increasing evidence for long-range chromatin loops between promoters and enhancers formed through specific DNA sequences, including tethering elements that bind the GAGA-associated factor (GAF). This study investigates the role of GAF as a looping factor in Drosophila development and finds that the N-terminal POZ/BTB oligomerization domain is crucial for the associations of distant tethering elements responsible for coordinating the activities of distant paralogous genes.
Article
Chemistry, Multidisciplinary
Benjamin C. Buchmuller, Jessica Droeden, Himanshu Singh, Shubhendu Palei, Malte Drescher, Rasmus Linser, Daniel Summerer
Summary: This study evolved a mutant protein capable of specific binding to methylated and hydroxymethylated cytosines, allowing for the identification and enrichment of individual CpG dyads.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Daisylyn Senna Tan, Shun Lai Cheung, Ya Gao, Maike Weinbuch, Haoqing Hu, Liyang Shi, Shih-Chieh Ti, Andrew P. Hutchins, Vlad Cojocaru, Ralf Jauch
Summary: Oct4 is found to bind to a compact palindromic DNA element with a methylated CpG core (CpGpal) in alternative states of pluripotency and during cellular reprogramming towards induced pluripotent stem cells (iPSCs). This binding is Oct4-specific and driven by its POU-homeodomain. The POU-specific domain is detached from DNA during the binding process.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Wenli Bai, Melissa L. Wells, Wi S. Lai, Stephanie N. Hicks, Adam B. Burkholder, Lalith Perera, Alan R. Kimmel, Perry J. Blackshear
Summary: Post-transcriptional processes mediated by mRNA-binding proteins are critical control points in gene expression. Dictyostelium discoideum may regulate expression of functionally related proteins through a single RNA-binding protein, TtpA, forming an "RNA regulon".
NUCLEIC ACIDS RESEARCH
(2021)
Review
Pharmacology & Pharmacy
Sawako Shindo, Satoru Kakizaki, Toshiyuki Sakaki, Yuki Kawasaki, Tsutomu Sakuma, Masahiko Negishi, Ryota Shizu
Summary: Nuclear receptors collectively regulate biological functions and have diverse roles beyond transcription activation. While most are activated by ligand binding, some are phosphorylated. The physiological relevance of phosphorylation in nuclear receptor activity has been confirmed, especially within conserved domains. This review focuses on estrogen and androgen receptors and highlights phosphorylation as a potential drug target.
PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Monika J. Stankiewicz, Jian Du, Dominick Martinico, Steven J. Ackerman
Summary: C/EBP epsilon(27) acts as a potent repressor of MBP1 transcription in eosinophil differentiation by interacting with GATA-1 protein and binding to a C/EBP site in the MBP1 promoter.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Lijing Ma, Zeraye Mehari Haile, Silvia Sabbadini, Bruno Mezzetti, Francesca Negrini, Elena Baraldi
Summary: FaMBL1 plays a role in the defense of strawberry plants against fungal pathogens. Overexpression of FaMBL1 in octoploid strawberries can reduce their susceptibility to anthracnose and grey mould. This study provides valuable insights into the defense mechanisms of strawberry plants and contributes to the understanding of their disease resistance.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Chemistry, Medicinal
Christoph Oehlknecht, Sonja Katz, Christina Kroess, Bernhard Sprenger, Petra Engele, Rainer Schneider, Chris Oostenbrink
Summary: The article introduces an efficient method for calculating protein binding free energies by combining the one-step perturbation and the third-power fitting approach. Experimental in silico saturation mutagenesis confirms the method's high accuracy in predicting relative free energies of amino acid mutations.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
