Article
Agronomy
Sijing Wan, Jianyun He, Lei Chao, Zuokun Shi, Shasha Wang, Weidong Yu, Zhen Huang, Su Wang, Shigui Wang, Zhijun Zhang
Summary: In this study, the relative survival ability, trehalose and glycogen contents, trehalose activity and trehalose metabolism-related gene-expression profiles were compared in a natural overwintering population and an indoor laboratory population of Harmonia axyridis. Yellow adults dominated the overwintering population, which had higher survival rates and trehalose levels during cold storage compared to the laboratory population. These findings suggest that yellow adults have a better ability to regulate trehalose metabolism under cold storage.
Article
Multidisciplinary Sciences
Changying Zheng, Lijuan Sun
Summary: The newly discovered posttranslational modification Kcr was found in ovarian tissue of Harmonia axyridis, affecting various biological processes and metabolism pathways, especially in carbohydrate and energy metabolism processes. Kcr may be involved in diapause regulation and ovary development in H. axyridis, with potential links to disease progression.
Article
Genetics & Heredity
Dongxiao Ma, Qin Zhang, Jintao Zhou, Yu Lu, Xiaomeng Duan, Chengzhong He, Jinde Yu
Summary: In this study, the expression stability of 10 candidate reference genes was analyzed in Toxicodendron succedaneum using RT-qPCR. The results showed that TsRG03 and ACT were recommended as suitable reference genes for all samples. These findings provide alternative reference genes for gene function studies, correction and normalization of target gene expression, and directed molecular breeding in T. succedaneum.
Article
Plant Sciences
Leandro Francisco de Oliveira, Amanda Rusiska Piovezani, Dimitre A. Ivanov, Leonardo Yoshida, Eny Iochevet Segal Floh, Massuo Jorge Kato
Summary: This study identified stable reference genes for gene expression analysis in four Piper species and provided a list of recommended gene pairs for each species. These findings will facilitate further transcriptomic and functional gene research.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Judit Tajti, Magda Pal, Tibor Janda
Summary: Oat, a widely cultivated cereal with high nutritional value, shows different stability of reference gene expression under diverse abiotic stress conditions. ADPR was identified as the top-rated reference gene for all samples, while TUB and EF1 were most affected by the treatments.
Article
Biochemistry & Molecular Biology
Alice Nevone, Francesca Lattarulo, Monica Russo, Giada Panno, Paolo Milani, Marco Basset, Maria Antonietta Avanzini, Giampaolo Merlini, Giovanni Palladini, Mario Nuvolone
Summary: In the next-generation sequencing era, RT-qPCR remains widely used for quantifying nucleic acids due to its popularity, versatility, and cost-effectiveness. However, accurate measurement of transcriptional levels requires appropriate reference genes for normalization. Here, we present a strategy that utilizes publicly available transcriptomic datasets to select suitable reference genes for specific clinical or experimental settings and includes a pipeline for RT-qPCR assay design and validation. We demonstrated the efficacy of this strategy by identifying and validating reference genes for transcriptional studies of bone-marrow plasma cells in AL amyloidosis patients. This strategy can be applied to other settings with publicly available transcriptomic datasets.
Article
Environmental Sciences
Ju Mu, Yu Wang, Miao Wang, Dayi Zhang, Mingying Liu
Summary: This study constructed a mouse model exposed to poly-propylene microplastics (PP-MPs) to evaluate the bioaccumulation of PP-MPs in the kidney and explore potential reference genes using RT-qPCR. The stability and reliability of 19 candidate reference genes were assessed using four algorithms, and the top five most stable genes were determined (Cox4i1, Rps3, Rps9, Rps18, and Gapdh). Three genes associated with different biochemical processes were chosen to verify the identified reference genes, showing different expression profiles and emphasizing the importance of selecting reliable reference genes.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Agriculture, Dairy & Animal Science
Darija Lemic, Ivana Pajac Zivkovic, Matea Sulicek, Hugo A. Benitez
Summary: This study demonstrates the efficiency of using geometric morphometrics to identify invasive forms of Harmonia axyridis, revealing minimal shape plasticity in wing shape and patterns of sexual shape dimorphism among invasive forms. Significant differences in wing shapes between forms in Croatia were identified, with each form having its own specific morphotype likely influenced by genetic changes in the species. Additional studies on a larger scale are necessary for further clarity.
Article
Genetics & Heredity
Zhipeng Zhu, Jianxiang Yu, Xinhui Tang, Aisheng Xiong, Miao Sun
Summary: In this study, 18 potential reference genes from okra were selected and their expression levels under different tissues and abiotic stress conditions were determined using Real-Time quantitative PCR. The most stable genes under cold, salt and drought stress were identified, providing a molecular basis for functional gene analysis and regulatory mechanism research of okra.
Article
Plant Sciences
Xin Liu, Yingbo Gao, Xinyi Zhao, Xiaoxiang Zhang, Linli Ben, Zongliang Li, Guichun Dong, Juan Zhou, Jianye Huang, Youli Yao
Summary: This study identified suitable reference genes for different rice tissues under drought and salt stress by using RNA-seq datasets. The results showed that OsMED7, OsACT1, and OsOS-9 were the robust reference genes for leaf samples, OsACT1, OsZOS3-23, and OsGDCP were recommended for shoots, and OsMED7, OsOS-9, and OsGDCP were the most reliable reference genes for roots. These findings are valuable for improving the accuracy of gene expression in rice under similar conditions through the selection of appropriate reference genes.
Article
Biochemistry & Molecular Biology
Janina Gospodarek, Agnieszka Krajewska, Iwona B. Pasmionka
Summary: Peppermint essential oil shows promising insecticidal efficacy against aphids and young larvae of the Colorado potato beetle, but it is toxic to young larvae of the non-target organism ladybug. Lower concentrations of the oil should be used to protect ladybug safety.
Article
Agronomy
Guoyong Li, Xiaoli Ni, Meikun Liu, Bing Tang, Can Li, Yangyang Liu
Summary: This study investigated the physiological adaptation and regulation ability of Harmonia axyridis, the dominant natural enemy of aphids, to chemical pesticide stress. The findings revealed that different pesticides had varying effects on the growth and development, food consumption, trehalase activity, and trehalose content of ladybirds.
Article
Environmental Sciences
Michael Meissle, Stefanie Kloos, Joerg Romeis
Summary: Insects such as lady beetles can ingest Cry proteins from genetically engineered crops without significant accumulation in their bodies, and the proteins are quickly degraded and excreted. The impact of Bt maize on lady beetle larvae and adults is minimal in terms of Cry protein exposure.
ENVIRONMENTAL POLLUTION
(2021)
Article
Plant Sciences
Maria Noelia Ulrich, Esteban Muniz-Padilla, Alejandra Corach, Esteban Hopp, Daniela Tosto
Summary: This study evaluated the stability of different reference genes of glyphosate-resistant S. halepense, finding that PP2A and ARI8 were the most stable genes. The results provide valuable information for gene expression analysis under glyphosate stress and will facilitate resistance mechanism studies in this weed species.
Article
Plant Sciences
Qizhang Wang, Chunqian Guo, Shipeng Yang, Qiwen Zhong, Jie Tian
Summary: The aim of this study was to identify suitable reference genes for gene expression analysis during different growth conditions in garlic. Nine candidate reference genes were selected using garlic transcriptome sequence data and their expression levels were evaluated in specific tissues under drought and cold stress. Several statistical methods were used to assess the stability of the reference gene expression levels. The most stable reference genes were identified, and their reliability was confirmed by evaluating the expression of a stress-responsive gene. This study provides a theoretical reference for gene expression analysis in garlic under stress conditions.
