Article
Multidisciplinary Sciences
Chao Ji, Xing Wang, Kai He, Yanhua Xue, Yahui Li, Liwei Xin, Wei Zhao, Jinshou Tian, Liang Sheng
Summary: Compressed Fluorescence Lifetime Imaging (Compressed-FLIM) is a novel method for single-shot widefield FLIM, with high temporal resolution and deep frame sequences. To improve the reconstruction accuracy, researchers developed a more effective combined prior model and conducted extensive numerical simulations and experiments to demonstrate its performance.
Article
Chemistry, Analytical
Mozhgan Samandarsangari, Daria O. Kozina, Victor V. Sokolov, Anastasia D. Komarova, Marina V. Shirmanova, Ilya S. Kritchenkov, Sergey P. Tunik
Summary: In this study, new phosphorescent iridium complexes were synthesized with water-soluble and biocompatible properties. The major photophysical characteristics were determined by two cyclometallating ligands based on 2-pyridine-benzothiophene. The use of various diimine ligands influenced the quantum yield of phosphorescence and the sensitivity to oxygen. The complexes demonstrated low toxicity and good water solubility.
Article
Engineering, Environmental
Adrian Monteleone, Folker Wenzel, Heinz Langhals, Daniel Dietrich
Summary: The FLIM method was tested for identification and characterization of six types of plastics, showing significant differentiation in 94.55% of the comparisons. Results suggest that FLIM has the potential for sub-micrometer plastic characterization and allows for visual 3D-sectioning of samples, which could be important for identifying and characterizing plastics in tissue and environmental samples.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Biochemical Research Methods
P. Wang, F. Hecht, G. Ossato, S. Tille, S. E. Fraser, J. A. Junge
Summary: Fluorescence lifetime imaging microscopy (FLIM) with phasor analysis allows for easy visualization and analysis of fluorophores' lifetimes, but typically suffers from low photon budgets leading to poor signal to noise ratios. Traditional median filters used in phasor analysis degrade high spatial frequency FLIM information, especially edges and puncta. A new filtering strategy using complex wavelet filtering and Anscombe transform preserves fine structures and accurate lifetimes in photon starved FLIM imaging, outperforming median filters and enabling FLIM imaging with lower laser power and faster imaging speed.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Biochemical Research Methods
Rupsa Datta, Amani Gillette, Matthew Stefely, Melissa C. Skala
Summary: Fluorescence lifetime imaging microscopy (FLIM) is a powerful molecular imaging technique that measures the decay rate of fluorophores to provide insights into molecular interactions. Major advances in FLIM instrumentation, analysis, and biological and clinical applications over the last year have contributed to the growing interest in FLIM and continuous technological improvements in biomedical research.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Biochemical Research Methods
Zece Zhu, Ye Sun, Teng Ma, Di Tian, Jintao Zhu
Summary: Luminescence lifetime imaging is crucial in distinguishing luminescence decay rates. A simple and accessible optical system using a smartphone was presented for detecting lifetimes of persistent luminescence. The system simplifies apparatus, reduces cost, and shows potential for time-resolved luminescence imaging in biological detection.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Jihoon Kang, Ha Lim Lee, Won Jae Chung, Jun Yeob Lee
Summary: High triplet energy hosts for blue phosphorescent OLEDs were developed by decorating a meta linked bitriazine core with carbazole and tetraphenylsilyl functional groups. Two hosts, one symmetric and one asymmetric, were prepared. The asymmetric host with a carbazole unit and a tetraphenylsilyl unit exhibited higher external quantum efficiency and better long-term device stability compared to the symmetric host with two carbazole units. A high EQE of 19.7% and a long device lifetime of 2093.6 h at 100 cd m-2 were achieved using the asymmetric host.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Martin Priessner, Peter A. Summers, Benjamin W. Lewis, Magdalena Sastre, Liming Ying, Marina K. Kuimova, Ramon Vilar
Summary: A new optical probe based on BODIPY has been developed, which exhibits fluorescence intensity switch-on upon binding to copper(I) and can be used to visualize copper(I) pools in lysosomes of live cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Biochemical Research Methods
Brian W. Pogue, Rongxiao Zhang, Xu Cao, Jeremy Mengyu Jia, Arthur Petusseau, Petr Bruza, Sergei A. Vinogradov
Summary: X-rays can penetrate deep tissues to induce optical responses of specific molecular reporters, offering a new way to sense and image tissue function in vivo. Advances in this field include the invention of biocompatible probes and innovations in optimizing the use of x-ray sources.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Optics
Lukas Klein, Arne S. Kristoffersen, Jan Tous, Karel Zidek
Summary: This article introduces a versatile microscope based on single-pixel imaging that can measure hyperspectral information and fluorescence lifetime imaging simultaneously. The microscope has potential applications in material characterization studies at both macro- and micro-levels.
Review
Chemistry, Multidisciplinary
Xiaoyong Jia, Liangliang Zhu
Summary: Photodriven self-assembly is a crucial strategy to regulate the molecular orderliness and structure. While traditional photochemical self-assembly has disadvantages, physical processes based on photoexcitation can fully utilize photons to avoid drawbacks. These processes drive molecular movement and aggregation, promoting the assembly of materials. The exploration of photoexcitation-induced assembly can lead to the development of unprecedented optoelectronic materials.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Evgeny A. Shirshin, Marina V. Shirmanova, Alexey V. Gayer, Maria M. Lukina, Elena E. Nikonova, Boris P. Yakimov, Gleb S. Budylin, Varvara V. Dudenkova, Nadezhda I. Ignatova, Dmitry V. Komarov, Vladislav V. Yakovlev, Wolfgang Becker, Elena V. Zagaynova, Vladislav I. Shcheslavskiy, Marlan O. Scully
Summary: The heterogeneity of cells determines their response to external factors, and the metabolic heterogeneity of tumor cells is of clinical relevance. Fluorescence lifetime imaging (FLIM) is a non-invasive technique that can sense and quantify cellular metabolism by measuring the fluorescence decay parameters of endogenous fluorophores. This study demonstrates the unique capability of FLIM in accurately discriminating metabolic heterogeneity using a non-parametric analysis approach, which has been validated in cultured cancer cells treated with chemotherapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Engineering, Environmental
Lisong Deng, Zetong Ma, Jiadong Zhou, Liangjian Chen, Junjie Wang, Xianfeng Qiao, Dehua Hu, Dongge Ma, Junbiao Peng, Yuguang Ma
Summary: This paper presents a novel phosphor system based on BTPO that exhibits ultralong RTP and high quantum efficiency in a polymer matrix. The efficient and multichannel intersystem crossing process, as well as the small radiative rate, contribute to the extended lifetime of the phosphor.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Materials Science, Multidisciplinary
Andreas Russegger, Angela C. Debruyne, Daniel Carvajal Berrio, Stefanie Fuchs, Julia Marzi, Katja Schenke-Layland, Ruslan I. Dmitriev, Sergey M. Borisov
Summary: Luminescence thermometry is a preferred technique for temperature measurements and imaging of temperature distribution in small objects. However, current luminescent probes have limitations in terms of spectral characteristics, brightness, photostability, and sensitivity. A new generation of molecular thermometers using stable zirconium(IV) pyridinedipyrrolide complexes can overcome these limitations. These dyes emit pure thermally activated delayed fluorescence, have visible light excitation and emission, high luminescence brightness, excellent photostability, and suitability for two-photon excitation. They also exhibit mono-exponential decay with temperaturesensitive lifetimes. Immobilization in gas-blocking polymers allows the manufacturing of self-referenced sensing materials in the forms of planar optodes and water-dispersible nanoparticles. Positively charged nanoparticles can be used for nanothermometry in live cells and multicellular spheroids, while negatively charged nanoparticles are useful for imaging temperature gradients in small volume samples.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Biochemical Research Methods
Miriam C. Bassler, Tim Rammler, Frank Wackenhut, Sven Zur Oven-Krockhaus, Ivona Secic, Rainer Ritz, Alfred J. Meixner, Marc Brecht
Summary: This study investigates the distribution and penetration behavior of hypericin in glioma cell spheroids using fluorescence microscopy and FLIM. The results show that hypericin exhibits a gradient distribution towards the spheroid core for short incubation periods or small concentrations, while a homogeneous distribution is observed for long incubation times or high concentrations.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Joao L. Lagarto, Caterina Credi, Federica Villa, Simone Tisa, Franco Zappa, Vladislav Shcheslavskiy, Francesco Saverio Pavone, Riccardo Cicchi
Article
Biochemical Research Methods
Boris P. Yakimov, Maria A. Gogoleva, Alexey N. Semenov, Sergey A. Rodionov, Marina V. Novoselova, Alexey V. Gayer, Alexey V. Kovalev, Alexey I. Bernakevich, Victor V. Fadeev, Artashes G. Armaganov, Vladimir P. Drachev, Dmitry A. Gorin, Maxim E. Darvin, Vladislav I. Shcheslavskiy, Gleb S. Budylin, Alexander V. Priezzhev, Evgeny A. Shirshin
BIOMEDICAL OPTICS EXPRESS
(2019)
Article
Biochemical Research Methods
Joao L. Lagarto, Vladislav Shcheslavskiy, Francesco S. Pavone, Riccardo Cicchi
JOURNAL OF BIOPHOTONICS
(2020)
Article
Optics
Joao L. Lagarto, Vladislav Shcheslavskiy, Francesco Saverio Pavone, Riccardo Cicchi
Article
Biochemical Research Methods
Svetlana Rodimova, Daria Kuznetsova, Nikolai Bobrov, Vadim Elagin, Vladislav Shcheslavskiy, Vladimir Zagainov, Elena Zagaynova
BIOMEDICAL OPTICS EXPRESS
(2020)
Correction
Optics
Pavel Morozov, Maria Lukina, Marina Shirmanova, Alexander Divochiy, Varvara Dudenkova, Gregory N. Gol'tsman, Wolfgang Becker, Vladislav I. Shcheslavskiy
Article
Engineering, Electrical & Electronic
Svetlana A. Rodimova, Daria S. Kuznetsova, Nikolai Bobrov, Alexander A. Gulin, Dmitry G. Reunov, Maria M. Karabut, Vladislav Shcheslavskiy, Natalia V. Vdovina, Vladimir E. Zagainov, Elena Zagaynova
Summary: In this study, liver tissue during the regeneration process was investigated using multiphoton microscopy, FLIM, second-harmonic generation microscopy, and mass spectrometry analysis. Results showed an increase in bound forms of NAD(P)H during liver regeneration, indicating metabolic shift towards oxidative phosphorylation and increased biosynthesis processes in hepatocytes. The approach used in this work is effective for analyzing the dynamics of changes in the structural and functional state of liver tissue during regeneration.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2021)
Article
Engineering, Electrical & Electronic
Maria M. Lukina, Marina A. Sirotkina, Anna G. Orlova, Varvara V. Dudenkova, Anastasia D. Komarova, Anton A. Plekhanov, Ludmila B. Snopova, Elena V. Zagaynova, Dmitri B. Papkovsky, Vladislav I. Shcheslavskiy, Marina V. Shirmanova
Summary: The study examined the effects of irinotecan on tumor angiogenesis and oxygen content in a mouse model of colorectal cancer, revealing that irinotecan reduces tumor oxygenation and blood perfusion through an antiangiogenic mechanism of action. Immunohistochemistry confirmed these findings.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2021)
Article
Multidisciplinary Sciences
Liubov Shimolina, Maria Lukina, Vladislav Shcheslavskiy, Vadim Elagin, Varvara Dudenkova, Nadezhda Ignatova, Marina K. Kuimova, Marina Shirmanova
Summary: Viscosity is an essential physical property of biological membranes, playing a key role in regulating the morphological and physiological state of living cells. Tumor cells exhibit significant alterations in their plasma membrane composition and structure, aiding in their resistance to drug therapies and adaptation to hostile microenvironments. Techniques such as fluorescence lifetime imaging microscopy (FLIM) can be used to study the links between membrane viscosity and metabolic profile of cancer cells, offering insights into the effects of chemotherapy.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Biophysics
Liubov Shimolina, Ekaterina Potekhina, Irina Druzhkova, Maria Lukina, Varvara Dudenkova, Vsevolod Belousov, Vladislav Shcheslavskiy, Elena Zagaynova, Marina Shirmanova
Summary: Changes in intracellular pH reflect the metabolic states of cancer cells during tumor growth and dissemination. Monitoring pH is essential for understanding the metabolic mechanisms supporting cancer progression. Genetically encoded fluorescent pH sensors provide real-time tracking of pH in specific subcellular compartments, but their ratiometric readout is not suitable for thick samples ex vivo or tissues in vivo, including solid tumors. Fluorescence lifetime imaging (FLIM) offers a promising alternative to conventional fluorescence microscopy. This study presents a quantitative approach to map pH in cancer cells and tumors in vivo using the fluorescence lifetime of a genetically encoded pH sensor called SypHerRed. The study demonstrates the utility of SypHerRed in visualizing pH in cancer cell culture and mouse tumor xenografts using FLIM and macroscopy. For the first time, the absolute pH value is obtained for tumors in vivo using an optical technique. Additionally, the study shows the simultaneous detection of pH and the endogenous fluorescence of metabolic cofactor NADH, providing complementary insights into the metabolic aspects of cancer. The fluorescence lifetime-based readout and red-shifted spectra of SypHerRed make it a promising instrument for multiparameter in vivo imaging applications.
BIOPHYSICAL JOURNAL
(2022)
Article
Multidisciplinary Sciences
Evgeny A. Shirshin, Marina V. Shirmanova, Alexey V. Gayer, Maria M. Lukina, Elena E. Nikonova, Boris P. Yakimov, Gleb S. Budylin, Varvara V. Dudenkova, Nadezhda I. Ignatova, Dmitry V. Komarov, Vladislav V. Yakovlev, Wolfgang Becker, Elena V. Zagaynova, Vladislav I. Shcheslavskiy, Marlan O. Scully
Summary: The heterogeneity of cells determines their response to external factors, and the metabolic heterogeneity of tumor cells is of clinical relevance. Fluorescence lifetime imaging (FLIM) is a non-invasive technique that can sense and quantify cellular metabolism by measuring the fluorescence decay parameters of endogenous fluorophores. This study demonstrates the unique capability of FLIM in accurately discriminating metabolic heterogeneity using a non-parametric analysis approach, which has been validated in cultured cancer cells treated with chemotherapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Marina Shirmanova, Alena Gavrina, Tatiana F. Kovaleva, Varvara V. Dudenkova, Ekaterina E. Zelenova, Vladislav Shcheslavskiy, Artem M. Mozherov, Ludmila B. Snopova, Konstantin A. Lukyanov, Elena Zagaynova
Summary: Cellular redox status and ROS level are closely related to the apoptotic potential of cancer cells. This study investigates the correlation between ROS production and redox cofactors using fluorescence imaging. The results suggest new therapeutic strategies based on cellular redox balance regulation to improve anti-tumor activity and overcome apoptotic resistance.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Yulia P. Parshina, Anastasia D. Komarova, Leonid N. Bochkarev, Tatyana A. Kovylina, Anton A. Plekhanov, Larisa G. Klapshina, Aleksey N. Konev, Artem M. Mozherov, Ilya D. Shchechkin, Marina A. Sirotkina, Vladislav Shcheslavskiy, Marina Shirmanova
Summary: Tumor cells are able to grow in conditions of variable oxygen supply and hypoxia by switching between different metabolic pathways. This study develops a methodology for analyzing the metabolic status and oxygen level of tumor cells simultaneously. The results reveal a high degree of heterogeneity in both metabolic and oxygen states of mouse tumors, with a lower dependence of metabolism on oxygen compared to in vitro conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Optics
Vladislav I. Shcheslavskiy, Diana V. Yuzhakova, Daria A. Sachkova, Marina Shirmanova, Wolfgang Becker
Summary: This study presents a laser scanning system for macroscopic samples that can record fully resolved decay curves and images at multiple laser wavelengths. The system utilizes confocal detection to eliminate lateral scattering and out-of-focus haze. The system's performance is demonstrated on model experiments and a tumor model in living mice.
Article
Chemistry, Multidisciplinary
Ilya S. Kritchenkov, Anastasiia A. Elistratova, Viktor V. Sokolov, Pavel S. Chelushkin, Marina Shirmanova, Maria M. Lukina, Varvara V. Dudenkova, Vladislav Shcheslayskiy, Sviatlana Kalinina, Kirsten Reess, Angelika Rueck, Sergey P. Tunik
NEW JOURNAL OF CHEMISTRY
(2020)
