Journal
FLORIDA ENTOMOLOGIST
Volume 102, Issue 2, Pages 353-358Publisher
FLORIDA ENTOMOLOGICAL SOC
DOI: 10.1653/024.102.0210
Keywords
white-backed planthopper; host plant choice; gene silencing; olfactory protein
Categories
Funding
- National Natural Science Foundation of China [31572005]
- Research Foundation of Education Bureau of Hunan Province, China [15A090]
- Hunan Provincial Postgraduate Research and Innovation Project of China [CX2017B350]
Ask authors/readers for more resources
Southern rice black-streaked dwarf virus, transmitted by the white-backed planthopper (Sogatella furcifera [Horvath]) (Hemiptera: Delphacidae) was first found in Guangdong Province, China. A previous study has demonstrated that the host plant preferences of S. furcifera are altered by infection with the virus, with virus-free S. furcifera preferring virus-infected rice plants over healthy rice plants, whereas viruliferous S. furcifera prefer uninfected plants. To test the hypothesis that odorant-binding proteins (OBPs) are involved in the preference of S. furcifera for virus-infected rice plants, we first compared the expression levels of SfurOBP2 and SfurOBP11 in virus-free and viruliferous S. furcifera. The results show that mRNA transcript of these 2 genes were significantly reduced in viruliferous S. furcifera. We then used RNAi-mediated gene silencing to confirm the function of these 2 odorant-binding proteins in host selection of S. furcifera. The results showed that silencing of the SfurOBP2 gene caused virus-free S. furcifera to no longer prefer virus-infected rice plants over uninfected rice plants, but the ability to locate host plants was maintained. These results indicate that SfurOBP2 appears to play a crucial role in the preference of S. furcifera for virus-infected rice plants.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available