Article
Biochemistry & Molecular Biology
Yuan Liu, Ao Zhang, Na Guo, Qiang Hao, Fuhua Li
Summary: In this study, a ficolin gene Ptficolin was characterized from the swimming crab Portunus trituberculatus. Ptficolin showed higher expression in stomach and hepatopancreas, and could recognize and eliminate invading pathogens, playing an important role in hemolymph coagulation in crabs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Fisheries
Mengqi Ni, Yi Zhang, Jinbin Zheng, Zhaoxia Cui
Summary: This study investigated the activation function of a type I heat shock protein 40 (PtHSP40-I) from Portunus trituberculatus in the TLR pathway. The results showed that PtHSP40-I can bind to lipopolysaccharide and peptidoglycan, and also exhibit binding activity towards the extracellular leucine-rich repeat domain of Toll-like receptors. Moreover, PtHSP40-I promotes the translocation of the transcription factor Dorsal and participates in regulating the expression of anti-lipopolysaccharide factor and crustin. These findings provide insights into the activation mechanisms of the TLR pathway mediated by HSP40 and have implications for disease control in P. trituberculatus aquaculture.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Environmental Sciences
Ao Zhang, Yuan Liu, Na Guo, Shihao Li, Fuhua Li
Summary: This study identified two LRR-only proteins, PtLRR1 and PtLRR2, in the swimming crab Portunus trituberculatus, which potentially act as immune receptors and regulate antibacterial immunity. PtLRR1 and PtLRR2 show different activities in inducing gene expression related to inflammation and the proPO system, and they can bind to various microbial molecules.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Xiaohan Liu, Jiqing Gao, Xiao Wei, Xinze Zhang, Liping You, Yuanjin Liu, Lizhu Chen, Caili Liu, Chunxiao Sun, Xiuhui Tian, Quanli Zhou, Xiaomin Zhang
Summary: In this study, the potential mechanisms of PAMP binding and opsonic activities of a c-type lectin and a sialic acid-binding lectin from manila clam were investigated. The results showed that both lectins could bind to various pathogen-associated molecular patterns (PAMPs) and enhance the phagocytosis and encapsulation ability of hemocytes. These lectins may function as pattern recognition receptors (PRRs) involved in the innate immune responses of the clam.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Infectious Diseases
Yuan Liu, Yue Su, Ao Zhang, Zhaoxia Cui
Summary: PtCLec2 was identified from Portunus trituberculatus and plays a role in defense and innate immunity through its binding and agglutination activities. It regulates hemocyte phagocytosis, antimicrobial peptide synthesis, and prophenoloxidase activation for pathogen elimination.
Article
Fisheries
Mengqi Ni, Yi Zhang, Jinbin Zheng, Zhaoxia Cui
Summary: In this study, a type IIa crustin named PtCrustin5 was cloned from Portunus trituberculatus, and its immune functions against bacteria were investigated. PtCrustin5 showed binding preference and inhibitory activity against gram-positive bacteria.
Article
Fisheries
Su-Ming Zhou, Jiao-Jiao Zhao, Yan Wang, Shan Jin, Qi-Cun Zhou, Fei Yin
Summary: In this study, a marine crab Pt-IMD was identified and found to be constitutively expressed in normal crab. Over-expression of Pt-IMD in Drosophila cells activated antimicrobial peptide genes. Knocking down of Pt-IMD in crab cells inhibited the expression of several antimicrobial peptides.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Ting Kang, Yanting Xia, Taiwei Dong, Xiaoyuan Zheng, Shun Yang, Shichao Qian, Mengmeng Huang, Hui Fei
Summary: A new C-type lectin, PtCTL-9, was identified from swimming crab and showed broad non-self immunity, acting as an opsonin to promote phagocytosis and hemocyte encapsulation. The QPN motif was found to be important for PtCTL-9's immune function.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Huimin Duan, Shuaiqi Yang, Xiangmin Zhang, Guangdong Ji
Summary: This study demonstrates the crucial role of receptor interacting protein kinase-1 in immune response regulation, including its direct interaction with bacteria, which plays a key role in anti-infectious responses.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2022)
Article
Environmental Sciences
Kejie Zhang, Yu Xin, Ce Shi, Zhijun Xie, Zhiming Ren
Summary: A novel individual re-identification method based on the Pyramidal Feature Fusion Model (PFFM) for the swimming crab Portunus trituberculatus was proposed, utilizing triplet loss to improve identification accuracy. Experimental results showed high performance of the proposed method under occlusion scenarios.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yi Zhang, Peng Zhang, Mengqi Ni, Bin Zhou, Yunhui Bai, Jinbin Zheng, Zhaoxia Cui
Summary: This study revealed the importance of spa spacing diaeresis tzle in activating the TLR signal pathway. Two spa spacing diaeresis tzle were cloned from Portunus trituberculatus, and they were found to be highly expressed in the gills. Their transcriptional levels rapidly increased in response to infection. Additionally, these two spa spacing diaeresis tzle could interact with various pathogen-associated molecules and directly interact with the LRR domain of TLR.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Multidisciplinary
Ummey Jannatun Nahar, Istvan Toth, Mariusz Skwarczynski
Summary: This article discusses the use of adjuvants and vaccine delivery systems to enhance vaccine efficacy. It focuses on the role of mannose receptors in antigen recognition by antigen presenting cells (APCs). The production strategies and effectiveness of mannosylated vaccines are examined, considering factors such as mannose valency, density, and routes of administration. The advantages, disadvantages, and future directions of mannosylation strategy are also discussed.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Immunology
Yi Zhang, Mengqi Ni, Yunhui Bai, Qiao Shi, Jinbin Zheng, Zhaoxia Cui
Summary: Previously, it was believed that invertebrates relied solely on their non-specific innate immune system to combat invading microorganisms. However, recent studies have shown that invertebrates actually have a diverse and specific innate immune response due to their hyper-variable immune molecules. In this study, a full-length transcriptome analysis of immune-related genes in Portunus trituberculatus was conducted, revealing the diversity of immune-related genes and their tissue distributions. Furthermore, the study also identified several candidates for antibiotic agents in the prevention and treatment of bacteriosis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Ying Huang, Ying Jiang, Miao-Miao Wang, Min-Yi Chen, Huan-Gen Chen, Heng-Yuan Chen, Wen-Jie Liu, Xiao-Rui Li, Xiao-Lei Han
Summary: This study cloned and characterized a mannose-binding lectin (MBL) called PcMBL from the crayfish Procambarus clarkii. PcMBL plays important roles in nonself recognition and clearance of invading microorganisms. It was found that PcMBL can influence the transcription of antimicrobial peptide genes and has the ability to bind to various microorganisms and carbohydrates. Furthermore, recombinant PcMBL can facilitate bacterial clearance, inhibit biofilm formation and growth, and reduce mortality in infected crayfish.
AQUACULTURE REPORTS
(2023)
Article
Environmental Sciences
Shisheng Tu, Fuqiang Ge, Yaoyao Han, Mengen Wang, Xi Xie, Dongfa Zhu
Summary: This study identifies the Crz neuropeptide and its receptor in the swimming crab, and demonstrates their potential roles in ecdysteroid synthesis and ovarian development.
FRONTIERS IN MARINE SCIENCE
(2022)